ERX2694221 E-MTAB-6910:Sample 13_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594530 SAMEA4774578 Sample 13_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type microglial cell ERX2694222 E-MTAB-6910:Sample 14_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594531 SAMEA4774579 Sample 14_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type microglial cell ERX2694223 E-MTAB-6910:Sample 10_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594532 SAMEA4774580 Sample 10_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type microglial cell ERX2694224 E-MTAB-6910:Sample 9_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594533 SAMEA4774581 Sample 9_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type microglial cell ERX2694225 E-MTAB-6910:Sample 15_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594534 SAMEA4774582 Sample 15_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa; tet-VEGF Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type microglial cell ERX2694226 E-MTAB-6910:Sample 16_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594535 SAMEA4774583 Sample 16_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa; tet-VEGF Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type microglial cell ERX2694227 E-MTAB-6910:Sample 11_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594536 SAMEA4774584 Sample 11_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa; tet-VEGF Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type microglial cell ERX2694228 E-MTAB-6910:Sample 12_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594537 SAMEA4774585 Sample 12_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Cx3cr1-GFP; Camk2a-tTa; tet-VEGF Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type microglial cell ERX2694229 E-MTAB-6910:Sample 5_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594538 SAMEA4774586 Sample 5_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2a-tTa Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type endothelial cell ERX2694230 E-MTAB-6910:Sample 6_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594539 SAMEA4774587 Sample 6_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2a-tTa Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type endothelial cell ERX2694231 E-MTAB-6910:Sample 1_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594540 SAMEA4774588 Sample 1_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2a-tTa Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type endothelial cell ERX2694232 E-MTAB-6910:Sample 2_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594541 SAMEA4774589 Sample 2_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2a-tTa Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type endothelial cell ERX2694233 E-MTAB-6910:Sample 7_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594542 SAMEA4774590 Sample 7_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2-tTa tet-VEGF Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type endothelial cell ERX2694234 E-MTAB-6910:Sample 8_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594543 SAMEA4774591 Sample 8_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2-tTa tet-VEGF Experimental Factor: organism part CA1 field of hippocampus Experimental Factor: cell type endothelial cell ERX2694235 E-MTAB-6910:Sample 3_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594544 SAMEA4774592 Sample 3_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2-tTa tet-VEGF Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type endothelial cell ERX2694236 E-MTAB-6910:Sample 4_s ERP109687 PRJEB27587 RNA-seq experiment of isolated endothelial and microglial cells from hippocampal DG and CA1 regions under control and VEGF overexpression conditions ERS2594545 SAMEA4774593 Sample 4_s RNA-Seq TRANSCRIPTOMIC PolyA hippocampi were taken from Cx3cr1-GFP mice (3-4 mice pooled together per group). DG and CA1 were surgically removed under a stereoscope and collected into RNAse-free cold PBS. Dissociation was done using a neural tissue dissociation kit (Miltenyi Biotec, 130-092-628) and the gentleMACSTM Dissociator according to the manufacturer's instructions. Myelin was removed using myelin removal beads II (Miltenyi Biotec, 130-096-733). The same procedure was done for endothelial cell sorting using Tie2-GFP mice. Sorting was done using BD Aria FACS and only GFP positive cells were collected. For endothelial cell sorting VEGF was switched-on (by tetracycline removal) for 1 months. For microglia sorting, VEGF was switched-on for 1 week. RNA was obtained using RNeasy plus Micro Kit (QIAGEN) according to manufacturer's guidelines The libraries were constructed using TrueSeq RNA V2 kit of Illumina http://www.illumina.com/products/truseq_rna_sample_prep_kit_v2.html. Illumina MiSeq Experimental Factor: genotype Tie2-GFP; Camk2-tTa tet-VEGF Experimental Factor: organism part dentate gyrus of hippocampal formation Experimental Factor: cell type endothelial cell