ERX2698473 E-MTAB-6978:DMBC11_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597989 SAMEA4778042 DMBC11_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 11 ERX2698474 E-MTAB-6978:DMBC12_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597990 SAMEA4778043 DMBC12_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 12 ERX2698475 E-MTAB-6978:DMBC17_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597991 SAMEA4778044 DMBC17_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 17 ERX2698476 E-MTAB-6978:DMBC21_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597992 SAMEA4778045 DMBC21_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 21 ERX2698477 E-MTAB-6978:DMBC22_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597993 SAMEA4778046 DMBC22_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 22 ERX2698478 E-MTAB-6978:DMBC28_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597994 SAMEA4778047 DMBC28_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 28 ERX2698479 E-MTAB-6978:DMBC29_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597995 SAMEA4778048 DMBC29_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 29 ERX2698480 E-MTAB-6978:DMBC33_s ERP109743 PRJEB27635 Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines grown in vitro. ERS2597996 SAMEA4778049 DMBC33_s WXS GENOMIC RANDOM Melanoma specimens were obtained during surgical intervetions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were subjected to Nextera tagmentation followed by index PCR, first hybridization, capture, washes, elution, second hybridization, capture, washes, elution and enrichment of DNA fragments. The samples were subjected to two rounds of exome enrichment, followed by real-time PCR quantification (KAPA SYBR FAST qPCR Kit) prior to sequencing. NextSeq 500 Experimental Factor: # of patient from who tumor was obtained 33