ERX2714479 E-MTAB-7037:HS_control_21930_10_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611471 SAMEA4791542 HS_control_21930_10_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_10 ERX2714480 E-MTAB-7037:HS_control_21930_100_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611472 SAMEA4791543 HS_control_21930_100_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_100 ERX2714481 E-MTAB-7037:HS_control_21930_101_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611473 SAMEA4791544 HS_control_21930_101_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_101 ERX2714482 E-MTAB-7037:HS_control_21930_102_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611474 SAMEA4791545 HS_control_21930_102_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_102 ERX2714483 E-MTAB-7037:HS_control_21930_103_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611475 SAMEA4791546 HS_control_21930_103_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_103 ERX2714484 E-MTAB-7037:HS_control_21930_104_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611476 SAMEA4791547 HS_control_21930_104_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_104 ERX2714485 E-MTAB-7037:HS_control_21930_105_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611477 SAMEA4791548 HS_control_21930_105_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_105 ERX2714486 E-MTAB-7037:HS_control_21930_106_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611478 SAMEA4791549 HS_control_21930_106_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_106 ERX2714487 E-MTAB-7037:HS_control_21930_107_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611479 SAMEA4791550 HS_control_21930_107_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_107 ERX2714488 E-MTAB-7037:HS_control_21930_108_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611480 SAMEA4791551 HS_control_21930_108_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_108 ERX2714489 E-MTAB-7037:HS_control_21930_109_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611481 SAMEA4791552 HS_control_21930_109_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_109 ERX2714490 E-MTAB-7037:HS_control_21930_11_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611482 SAMEA4791553 HS_control_21930_11_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_11 ERX2714491 E-MTAB-7037:HS_control_21930_110_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611483 SAMEA4791554 HS_control_21930_110_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_110 ERX2714492 E-MTAB-7037:HS_control_21930_111_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611484 SAMEA4791555 HS_control_21930_111_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_111 ERX2714493 E-MTAB-7037:HS_control_21930_112_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611485 SAMEA4791556 HS_control_21930_112_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_112 ERX2714494 E-MTAB-7037:HS_control_21930_113_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611486 SAMEA4791557 HS_control_21930_113_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_113 ERX2714495 E-MTAB-7037:HS_control_21930_114_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611487 SAMEA4791558 HS_control_21930_114_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_114 ERX2714496 E-MTAB-7037:HS_control_21930_115_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611488 SAMEA4791559 HS_control_21930_115_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_115 ERX2714497 E-MTAB-7037:HS_control_21930_116_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611489 SAMEA4791560 HS_control_21930_116_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_116 ERX2714498 E-MTAB-7037:HS_control_21930_117_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611490 SAMEA4791561 HS_control_21930_117_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_117 ERX2714499 E-MTAB-7037:HS_control_21930_118_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611491 SAMEA4791562 HS_control_21930_118_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_118 ERX2714500 E-MTAB-7037:HS_control_21930_119_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611492 SAMEA4791563 HS_control_21930_119_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_119 ERX2714501 E-MTAB-7037:HS_control_21930_12_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611493 SAMEA4791564 HS_control_21930_12_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_12 ERX2714502 E-MTAB-7037:HS_control_21930_122_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611494 SAMEA4791565 HS_control_21930_122_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_122 ERX2714503 E-MTAB-7037:HS_control_21930_124_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611495 SAMEA4791566 HS_control_21930_124_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_124 ERX2714504 E-MTAB-7037:HS_control_21930_125_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611496 SAMEA4791567 HS_control_21930_125_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_125 ERX2714505 E-MTAB-7037:HS_control_21930_126_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611497 SAMEA4791568 HS_control_21930_126_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_126 ERX2714506 E-MTAB-7037:HS_control_21930_127_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611498 SAMEA4791569 HS_control_21930_127_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_127 ERX2714507 E-MTAB-7037:HS_control_21930_128_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611499 SAMEA4791570 HS_control_21930_128_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_128 ERX2714508 E-MTAB-7037:HS_control_21930_129_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611500 SAMEA4791571 HS_control_21930_129_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_129 ERX2714509 E-MTAB-7037:HS_control_21930_13_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611501 SAMEA4791572 HS_control_21930_13_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_13 ERX2714510 E-MTAB-7037:HS_control_21930_130_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611502 SAMEA4791573 HS_control_21930_130_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_130 ERX2714511 E-MTAB-7037:HS_control_21930_131_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611503 SAMEA4791574 HS_control_21930_131_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_131 ERX2714512 E-MTAB-7037:HS_control_21930_132_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611504 SAMEA4791575 HS_control_21930_132_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_132 ERX2714513 E-MTAB-7037:HS_control_21930_134_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611505 SAMEA4791576 HS_control_21930_134_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_134 ERX2714514 E-MTAB-7037:HS_control_21930_135_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611506 SAMEA4791577 HS_control_21930_135_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_135 ERX2714515 E-MTAB-7037:HS_control_21930_136_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611507 SAMEA4791578 HS_control_21930_136_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_136 ERX2714516 E-MTAB-7037:HS_control_21930_137_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611508 SAMEA4791579 HS_control_21930_137_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_137 ERX2714517 E-MTAB-7037:HS_control_21930_138_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611509 SAMEA4791580 HS_control_21930_138_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_138 ERX2714518 E-MTAB-7037:HS_control_21930_139_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611510 SAMEA4791581 HS_control_21930_139_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_139 ERX2714519 E-MTAB-7037:HS_control_21930_14_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611511 SAMEA4791582 HS_control_21930_14_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_14 ERX2714520 E-MTAB-7037:HS_control_21930_140_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611512 SAMEA4791583 HS_control_21930_140_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_140 ERX2714521 E-MTAB-7037:HS_control_21930_141_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611513 SAMEA4791584 HS_control_21930_141_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_141 ERX2714522 E-MTAB-7037:HS_control_21930_142_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611514 SAMEA4791585 HS_control_21930_142_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_142 ERX2714523 E-MTAB-7037:HS_control_21930_144_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611515 SAMEA4791586 HS_control_21930_144_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_144 ERX2714524 E-MTAB-7037:HS_control_21930_146_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611516 SAMEA4791587 HS_control_21930_146_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_146 ERX2714525 E-MTAB-7037:HS_control_21930_147_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611517 SAMEA4791588 HS_control_21930_147_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_147 ERX2714526 E-MTAB-7037:HS_control_21930_148_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611518 SAMEA4791589 HS_control_21930_148_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_148 ERX2714527 E-MTAB-7037:HS_control_21930_149_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611519 SAMEA4791590 HS_control_21930_149_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_149 ERX2714528 E-MTAB-7037:HS_control_21930_15_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611520 SAMEA4791591 HS_control_21930_15_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_15 ERX2714529 E-MTAB-7037:HS_control_21930_150_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611521 SAMEA4791592 HS_control_21930_150_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_150 ERX2714530 E-MTAB-7037:HS_control_21930_151_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611522 SAMEA4791593 HS_control_21930_151_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_151 ERX2714531 E-MTAB-7037:HS_control_21930_152_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611523 SAMEA4791594 HS_control_21930_152_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_152 ERX2714532 E-MTAB-7037:HS_control_21930_153_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611524 SAMEA4791595 HS_control_21930_153_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_153 ERX2714533 E-MTAB-7037:HS_control_21930_154_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611525 SAMEA4791596 HS_control_21930_154_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_154 ERX2714534 E-MTAB-7037:HS_control_21930_155_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611526 SAMEA4791597 HS_control_21930_155_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_155 ERX2714535 E-MTAB-7037:HS_control_21930_156_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611527 SAMEA4791598 HS_control_21930_156_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_156 ERX2714536 E-MTAB-7037:HS_control_21930_157_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611528 SAMEA4791599 HS_control_21930_157_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_157 ERX2714537 E-MTAB-7037:HS_control_21930_158_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611529 SAMEA4791600 HS_control_21930_158_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_158 ERX2714538 E-MTAB-7037:HS_control_21930_16_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611530 SAMEA4791601 HS_control_21930_16_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_16 ERX2714539 E-MTAB-7037:HS_control_21930_160_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611531 SAMEA4791602 HS_control_21930_160_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_160 ERX2714540 E-MTAB-7037:HS_control_21930_161_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611532 SAMEA4791603 HS_control_21930_161_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_161 ERX2714541 E-MTAB-7037:HS_control_21930_162_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611533 SAMEA4791604 HS_control_21930_162_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_162 ERX2714542 E-MTAB-7037:HS_control_21930_163_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611534 SAMEA4791605 HS_control_21930_163_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_163 ERX2714543 E-MTAB-7037:HS_control_21930_164_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611535 SAMEA4791606 HS_control_21930_164_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_164 ERX2714544 E-MTAB-7037:HS_control_21930_165_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611536 SAMEA4791607 HS_control_21930_165_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_165 ERX2714545 E-MTAB-7037:HS_control_21930_166_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611537 SAMEA4791608 HS_control_21930_166_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_166 ERX2714546 E-MTAB-7037:HS_control_21930_167_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611538 SAMEA4791609 HS_control_21930_167_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_167 ERX2714547 E-MTAB-7037:HS_control_21930_168_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611539 SAMEA4791610 HS_control_21930_168_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_168 ERX2714548 E-MTAB-7037:HS_control_21930_17_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611540 SAMEA4791611 HS_control_21930_17_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_17 ERX2714549 E-MTAB-7037:HS_control_21930_170_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611541 SAMEA4791612 HS_control_21930_170_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_170 ERX2714550 E-MTAB-7037:HS_control_21930_171_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611542 SAMEA4791613 HS_control_21930_171_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_171 ERX2714551 E-MTAB-7037:HS_control_21930_172_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611543 SAMEA4791614 HS_control_21930_172_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_172 ERX2714552 E-MTAB-7037:HS_control_21930_173_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611544 SAMEA4791615 HS_control_21930_173_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_173 ERX2714553 E-MTAB-7037:HS_control_21930_174_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611545 SAMEA4791616 HS_control_21930_174_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_174 ERX2714554 E-MTAB-7037:HS_control_21930_175_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611546 SAMEA4791617 HS_control_21930_175_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_175 ERX2714555 E-MTAB-7037:HS_control_21930_176_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611547 SAMEA4791618 HS_control_21930_176_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_176 ERX2714556 E-MTAB-7037:HS_control_21930_177_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611548 SAMEA4791619 HS_control_21930_177_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_177 ERX2714557 E-MTAB-7037:HS_control_21930_178_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611549 SAMEA4791620 HS_control_21930_178_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_178 ERX2714558 E-MTAB-7037:HS_control_21930_179_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611550 SAMEA4791621 HS_control_21930_179_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_179 ERX2714559 E-MTAB-7037:HS_control_21930_18_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611551 SAMEA4791622 HS_control_21930_18_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_18 ERX2714560 E-MTAB-7037:HS_control_21930_180_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611552 SAMEA4791623 HS_control_21930_180_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_180 ERX2714561 E-MTAB-7037:HS_control_21930_181_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611553 SAMEA4791624 HS_control_21930_181_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_181 ERX2714562 E-MTAB-7037:HS_control_21930_182_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611554 SAMEA4791625 HS_control_21930_182_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_182 ERX2714563 E-MTAB-7037:HS_control_21930_183_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611555 SAMEA4791626 HS_control_21930_183_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_183 ERX2714564 E-MTAB-7037:HS_control_21930_184_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611556 SAMEA4791627 HS_control_21930_184_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_184 ERX2714565 E-MTAB-7037:HS_control_21930_185_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611557 SAMEA4791628 HS_control_21930_185_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_185 ERX2714566 E-MTAB-7037:HS_control_21930_186_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611558 SAMEA4791629 HS_control_21930_186_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_186 ERX2714567 E-MTAB-7037:HS_control_21930_187_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611559 SAMEA4791630 HS_control_21930_187_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_187 ERX2714568 E-MTAB-7037:HS_control_21930_188_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611560 SAMEA4791631 HS_control_21930_188_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_188 ERX2714569 E-MTAB-7037:HS_control_21930_189_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611561 SAMEA4791632 HS_control_21930_189_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_189 ERX2714570 E-MTAB-7037:HS_control_21930_190_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611562 SAMEA4791633 HS_control_21930_190_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_190 ERX2714571 E-MTAB-7037:HS_control_21930_191_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611563 SAMEA4791634 HS_control_21930_191_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_191 ERX2714572 E-MTAB-7037:HS_control_21930_192_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611564 SAMEA4791635 HS_control_21930_192_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_192 ERX2714573 E-MTAB-7037:HS_control_21930_194_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611565 SAMEA4791636 HS_control_21930_194_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_194 ERX2714574 E-MTAB-7037:HS_control_21930_196_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611566 SAMEA4791637 HS_control_21930_196_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_196 ERX2714575 E-MTAB-7037:HS_control_21930_197_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611567 SAMEA4791638 HS_control_21930_197_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_197 ERX2714576 E-MTAB-7037:HS_control_21930_198_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611568 SAMEA4791639 HS_control_21930_198_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_198 ERX2714577 E-MTAB-7037:HS_control_21930_199_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611569 SAMEA4791640 HS_control_21930_199_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_199 ERX2714578 E-MTAB-7037:HS_control_21930_200_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611570 SAMEA4791641 HS_control_21930_200_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_200 ERX2714579 E-MTAB-7037:HS_control_21930_201_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611571 SAMEA4791642 HS_control_21930_201_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_201 ERX2714580 E-MTAB-7037:HS_control_21930_202_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611572 SAMEA4791643 HS_control_21930_202_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_202 ERX2714581 E-MTAB-7037:HS_control_21930_203_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611573 SAMEA4791644 HS_control_21930_203_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_203 ERX2714582 E-MTAB-7037:HS_control_21930_204_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611574 SAMEA4791645 HS_control_21930_204_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_204 ERX2714583 E-MTAB-7037:HS_control_21930_205_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611575 SAMEA4791646 HS_control_21930_205_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_205 ERX2714584 E-MTAB-7037:HS_control_21930_206_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611576 SAMEA4791647 HS_control_21930_206_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_206 ERX2714585 E-MTAB-7037:HS_control_21930_207_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611577 SAMEA4791648 HS_control_21930_207_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_207 ERX2714586 E-MTAB-7037:HS_control_21930_208_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611578 SAMEA4791649 HS_control_21930_208_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_208 ERX2714587 E-MTAB-7037:HS_control_21930_209_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611579 SAMEA4791650 HS_control_21930_209_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_209 ERX2714588 E-MTAB-7037:HS_control_21930_210_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611580 SAMEA4791651 HS_control_21930_210_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_210 ERX2714589 E-MTAB-7037:HS_control_21930_212_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611581 SAMEA4791652 HS_control_21930_212_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_212 ERX2714590 E-MTAB-7037:HS_control_21930_213_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611582 SAMEA4791653 HS_control_21930_213_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_213 ERX2714591 E-MTAB-7037:HS_control_21930_214_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611583 SAMEA4791654 HS_control_21930_214_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_214 ERX2714592 E-MTAB-7037:HS_control_21930_215_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611584 SAMEA4791655 HS_control_21930_215_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_215 ERX2714593 E-MTAB-7037:HS_control_21930_216_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611585 SAMEA4791656 HS_control_21930_216_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_216 ERX2714594 E-MTAB-7037:HS_control_21930_219_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611586 SAMEA4791657 HS_control_21930_219_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_219 ERX2714595 E-MTAB-7037:HS_control_21930_220_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611587 SAMEA4791658 HS_control_21930_220_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_220 ERX2714596 E-MTAB-7037:HS_control_21930_221_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611588 SAMEA4791659 HS_control_21930_221_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_221 ERX2714597 E-MTAB-7037:HS_control_21930_222_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611589 SAMEA4791660 HS_control_21930_222_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_222 ERX2714598 E-MTAB-7037:HS_control_21930_223_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611590 SAMEA4791661 HS_control_21930_223_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_223 ERX2714599 E-MTAB-7037:HS_control_21930_224_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611591 SAMEA4791662 HS_control_21930_224_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_224 ERX2714600 E-MTAB-7037:HS_control_21930_225_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611592 SAMEA4791663 HS_control_21930_225_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_225 ERX2714601 E-MTAB-7037:HS_control_21930_226_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611593 SAMEA4791664 HS_control_21930_226_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_226 ERX2714602 E-MTAB-7037:HS_control_21930_227_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611594 SAMEA4791665 HS_control_21930_227_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_227 ERX2714603 E-MTAB-7037:HS_control_21930_228_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611595 SAMEA4791666 HS_control_21930_228_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_228 ERX2714604 E-MTAB-7037:HS_control_21930_229_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611596 SAMEA4791667 HS_control_21930_229_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_229 ERX2714605 E-MTAB-7037:HS_control_21930_230_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611597 SAMEA4791668 HS_control_21930_230_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_230 ERX2714606 E-MTAB-7037:HS_control_21930_231_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611598 SAMEA4791669 HS_control_21930_231_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_231 ERX2714607 E-MTAB-7037:HS_control_21930_232_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611599 SAMEA4791670 HS_control_21930_232_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_232 ERX2714608 E-MTAB-7037:HS_control_21930_233_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611600 SAMEA4791671 HS_control_21930_233_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_233 ERX2714609 E-MTAB-7037:HS_control_21930_234_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611601 SAMEA4791672 HS_control_21930_234_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_234 ERX2714610 E-MTAB-7037:HS_control_21930_235_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611602 SAMEA4791673 HS_control_21930_235_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_235 ERX2714611 E-MTAB-7037:HS_control_21930_236_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611603 SAMEA4791674 HS_control_21930_236_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_236 ERX2714612 E-MTAB-7037:HS_control_21930_237_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611604 SAMEA4791675 HS_control_21930_237_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_237 ERX2714613 E-MTAB-7037:HS_control_21930_238_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611605 SAMEA4791676 HS_control_21930_238_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_238 ERX2714614 E-MTAB-7037:HS_control_21930_239_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611606 SAMEA4791677 HS_control_21930_239_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_239 ERX2714615 E-MTAB-7037:HS_control_21930_240_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611607 SAMEA4791678 HS_control_21930_240_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_240 ERX2714616 E-MTAB-7037:HS_control_21930_242_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611608 SAMEA4791679 HS_control_21930_242_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_242 ERX2714617 E-MTAB-7037:HS_control_21930_246_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611609 SAMEA4791680 HS_control_21930_246_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_246 ERX2714618 E-MTAB-7037:HS_control_21930_247_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611610 SAMEA4791681 HS_control_21930_247_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_247 ERX2714619 E-MTAB-7037:HS_control_21930_248_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611611 SAMEA4791682 HS_control_21930_248_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_248 ERX2714620 E-MTAB-7037:HS_control_21930_249_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611612 SAMEA4791683 HS_control_21930_249_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_249 ERX2714621 E-MTAB-7037:HS_control_21930_250_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611613 SAMEA4791684 HS_control_21930_250_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_250 ERX2714622 E-MTAB-7037:HS_control_21930_251_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611614 SAMEA4791685 HS_control_21930_251_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_251 ERX2714623 E-MTAB-7037:HS_control_21930_252_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611615 SAMEA4791686 HS_control_21930_252_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_252 ERX2714624 E-MTAB-7037:HS_control_21930_253_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611616 SAMEA4791687 HS_control_21930_253_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_253 ERX2714625 E-MTAB-7037:HS_control_21930_254_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611617 SAMEA4791688 HS_control_21930_254_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_254 ERX2714626 E-MTAB-7037:HS_control_21930_255_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611618 SAMEA4791689 HS_control_21930_255_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_255 ERX2714627 E-MTAB-7037:HS_control_21930_256_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611619 SAMEA4791690 HS_control_21930_256_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_256 ERX2714628 E-MTAB-7037:HS_control_21930_257_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611620 SAMEA4791691 HS_control_21930_257_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_257 ERX2714629 E-MTAB-7037:HS_control_21930_258_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611621 SAMEA4791692 HS_control_21930_258_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_258 ERX2714630 E-MTAB-7037:HS_control_21930_259_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611622 SAMEA4791693 HS_control_21930_259_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_259 ERX2714631 E-MTAB-7037:HS_control_21930_260_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611623 SAMEA4791694 HS_control_21930_260_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_260 ERX2714632 E-MTAB-7037:HS_control_21930_261_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611624 SAMEA4791695 HS_control_21930_261_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_261 ERX2714633 E-MTAB-7037:HS_control_21930_262_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611625 SAMEA4791696 HS_control_21930_262_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_262 ERX2714634 E-MTAB-7037:HS_control_21930_263_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611626 SAMEA4791697 HS_control_21930_263_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_263 ERX2714635 E-MTAB-7037:HS_control_21930_264_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611627 SAMEA4791698 HS_control_21930_264_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_264 ERX2714636 E-MTAB-7037:HS_control_21930_266_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611628 SAMEA4791699 HS_control_21930_266_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_266 ERX2714637 E-MTAB-7037:HS_control_21930_267_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611629 SAMEA4791700 HS_control_21930_267_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_267 ERX2714638 E-MTAB-7037:HS_control_21930_268_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611630 SAMEA4791701 HS_control_21930_268_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_268 ERX2714639 E-MTAB-7037:HS_control_21930_269_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611631 SAMEA4791702 HS_control_21930_269_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_269 ERX2714640 E-MTAB-7037:HS_control_21930_270_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611632 SAMEA4791703 HS_control_21930_270_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_270 ERX2714641 E-MTAB-7037:HS_control_21930_271_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611633 SAMEA4791704 HS_control_21930_271_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_271 ERX2714642 E-MTAB-7037:HS_control_21930_272_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611634 SAMEA4791705 HS_control_21930_272_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_272 ERX2714643 E-MTAB-7037:HS_control_21930_273_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611635 SAMEA4791706 HS_control_21930_273_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_273 ERX2714644 E-MTAB-7037:HS_control_21930_274_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611636 SAMEA4791707 HS_control_21930_274_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_274 ERX2714645 E-MTAB-7037:HS_control_21930_275_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611637 SAMEA4791708 HS_control_21930_275_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_275 ERX2714646 E-MTAB-7037:HS_control_21930_276_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611638 SAMEA4791709 HS_control_21930_276_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_276 ERX2714647 E-MTAB-7037:HS_control_21930_277_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611639 SAMEA4791710 HS_control_21930_277_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_277 ERX2714648 E-MTAB-7037:HS_control_21930_278_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611640 SAMEA4791711 HS_control_21930_278_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_278 ERX2714649 E-MTAB-7037:HS_control_21930_279_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611641 SAMEA4791712 HS_control_21930_279_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_279 ERX2714650 E-MTAB-7037:HS_control_21930_280_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611642 SAMEA4791713 HS_control_21930_280_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_280 ERX2714651 E-MTAB-7037:HS_control_21930_281_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611643 SAMEA4791714 HS_control_21930_281_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_281 ERX2714652 E-MTAB-7037:HS_control_21930_282_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611644 SAMEA4791715 HS_control_21930_282_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_282 ERX2714653 E-MTAB-7037:HS_control_21930_283_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611645 SAMEA4791716 HS_control_21930_283_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_283 ERX2714654 E-MTAB-7037:HS_control_21930_284_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611646 SAMEA4791717 HS_control_21930_284_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_284 ERX2714655 E-MTAB-7037:HS_control_21930_285_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611647 SAMEA4791718 HS_control_21930_285_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_285 ERX2714656 E-MTAB-7037:HS_control_21930_286_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611648 SAMEA4791719 HS_control_21930_286_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_286 ERX2714657 E-MTAB-7037:HS_control_21930_287_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611649 SAMEA4791720 HS_control_21930_287_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_287 ERX2714658 E-MTAB-7037:HS_control_21930_288_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611650 SAMEA4791721 HS_control_21930_288_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_288 ERX2714659 E-MTAB-7037:HS_control_21930_289_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611651 SAMEA4791722 HS_control_21930_289_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_289 ERX2714660 E-MTAB-7037:HS_control_21930_29_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611652 SAMEA4791723 HS_control_21930_29_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_29 ERX2714661 E-MTAB-7037:HS_control_21930_290_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611653 SAMEA4791724 HS_control_21930_290_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_290 ERX2714662 E-MTAB-7037:HS_control_21930_293_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611654 SAMEA4791725 HS_control_21930_293_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_293 ERX2714663 E-MTAB-7037:HS_control_21930_294_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611655 SAMEA4791726 HS_control_21930_294_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_294 ERX2714664 E-MTAB-7037:HS_control_21930_295_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611656 SAMEA4791727 HS_control_21930_295_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_295 ERX2714665 E-MTAB-7037:HS_control_21930_296_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611657 SAMEA4791728 HS_control_21930_296_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_296 ERX2714666 E-MTAB-7037:HS_control_21930_297_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611658 SAMEA4791729 HS_control_21930_297_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_297 ERX2714667 E-MTAB-7037:HS_control_21930_298_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611659 SAMEA4791730 HS_control_21930_298_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_298 ERX2714668 E-MTAB-7037:HS_control_21930_299_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611660 SAMEA4791731 HS_control_21930_299_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_299 ERX2714669 E-MTAB-7037:HS_control_21930_300_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611661 SAMEA4791732 HS_control_21930_300_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_300 ERX2714670 E-MTAB-7037:HS_control_21930_301_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611662 SAMEA4791733 HS_control_21930_301_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_301 ERX2714671 E-MTAB-7037:HS_control_21930_302_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611663 SAMEA4791734 HS_control_21930_302_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_302 ERX2714672 E-MTAB-7037:HS_control_21930_303_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611664 SAMEA4791735 HS_control_21930_303_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_303 ERX2714673 E-MTAB-7037:HS_control_21930_304_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611665 SAMEA4791736 HS_control_21930_304_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_304 ERX2714674 E-MTAB-7037:HS_control_21930_305_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611666 SAMEA4791737 HS_control_21930_305_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_305 ERX2714675 E-MTAB-7037:HS_control_21930_306_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611667 SAMEA4791738 HS_control_21930_306_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_306 ERX2714676 E-MTAB-7037:HS_control_21930_307_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611668 SAMEA4791739 HS_control_21930_307_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_307 ERX2714677 E-MTAB-7037:HS_control_21930_308_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611669 SAMEA4791740 HS_control_21930_308_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_308 ERX2714678 E-MTAB-7037:HS_control_21930_309_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611670 SAMEA4791741 HS_control_21930_309_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_309 ERX2714679 E-MTAB-7037:HS_control_21930_310_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611671 SAMEA4791742 HS_control_21930_310_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_310 ERX2714680 E-MTAB-7037:HS_control_21930_311_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611672 SAMEA4791743 HS_control_21930_311_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_311 ERX2714681 E-MTAB-7037:HS_control_21930_312_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611673 SAMEA4791744 HS_control_21930_312_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_312 ERX2714682 E-MTAB-7037:HS_control_21930_314_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611674 SAMEA4791745 HS_control_21930_314_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_314 ERX2714683 E-MTAB-7037:HS_control_21930_315_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611675 SAMEA4791746 HS_control_21930_315_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_315 ERX2714684 E-MTAB-7037:HS_control_21930_316_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611676 SAMEA4791747 HS_control_21930_316_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_316 ERX2714685 E-MTAB-7037:HS_control_21930_317_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611677 SAMEA4791748 HS_control_21930_317_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_317 ERX2714686 E-MTAB-7037:HS_control_21930_318_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611678 SAMEA4791749 HS_control_21930_318_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_318 ERX2714687 E-MTAB-7037:HS_control_21930_319_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611679 SAMEA4791750 HS_control_21930_319_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_319 ERX2714688 E-MTAB-7037:HS_control_21930_320_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611680 SAMEA4791751 HS_control_21930_320_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_320 ERX2714689 E-MTAB-7037:HS_control_21930_321_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611681 SAMEA4791752 HS_control_21930_321_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_321 ERX2714690 E-MTAB-7037:HS_control_21930_322_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611682 SAMEA4791753 HS_control_21930_322_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_322 ERX2714691 E-MTAB-7037:HS_control_21930_323_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611683 SAMEA4791754 HS_control_21930_323_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_323 ERX2714692 E-MTAB-7037:HS_control_21930_324_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611684 SAMEA4791755 HS_control_21930_324_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_324 ERX2714693 E-MTAB-7037:HS_control_21930_325_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611685 SAMEA4791756 HS_control_21930_325_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_325 ERX2714694 E-MTAB-7037:HS_control_21930_326_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611686 SAMEA4791757 HS_control_21930_326_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_326 ERX2714695 E-MTAB-7037:HS_control_21930_327_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611687 SAMEA4791758 HS_control_21930_327_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_327 ERX2714696 E-MTAB-7037:HS_control_21930_328_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611688 SAMEA4791759 HS_control_21930_328_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_328 ERX2714697 E-MTAB-7037:HS_control_21930_329_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611689 SAMEA4791760 HS_control_21930_329_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_329 ERX2714698 E-MTAB-7037:HS_control_21930_33_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611690 SAMEA4791761 HS_control_21930_33_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_33 ERX2714699 E-MTAB-7037:HS_control_21930_330_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611691 SAMEA4791762 HS_control_21930_330_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_330 ERX2714700 E-MTAB-7037:HS_control_21930_331_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611692 SAMEA4791763 HS_control_21930_331_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_331 ERX2714701 E-MTAB-7037:HS_control_21930_332_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611693 SAMEA4791764 HS_control_21930_332_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_332 ERX2714702 E-MTAB-7037:HS_control_21930_333_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611694 SAMEA4791765 HS_control_21930_333_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_333 ERX2714703 E-MTAB-7037:HS_control_21930_334_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611695 SAMEA4791766 HS_control_21930_334_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_334 ERX2714704 E-MTAB-7037:HS_control_21930_335_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611696 SAMEA4791767 HS_control_21930_335_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_335 ERX2714705 E-MTAB-7037:HS_control_21930_336_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611697 SAMEA4791768 HS_control_21930_336_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_336 ERX2714706 E-MTAB-7037:HS_control_21930_34_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611698 SAMEA4791769 HS_control_21930_34_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_34 ERX2714707 E-MTAB-7037:HS_control_21930_340_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611699 SAMEA4791770 HS_control_21930_340_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_340 ERX2714708 E-MTAB-7037:HS_control_21930_341_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611700 SAMEA4791771 HS_control_21930_341_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_341 ERX2714709 E-MTAB-7037:HS_control_21930_342_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611701 SAMEA4791772 HS_control_21930_342_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_342 ERX2714710 E-MTAB-7037:HS_control_21930_343_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611702 SAMEA4791773 HS_control_21930_343_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_343 ERX2714711 E-MTAB-7037:HS_control_21930_344_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611703 SAMEA4791774 HS_control_21930_344_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_344 ERX2714712 E-MTAB-7037:HS_control_21930_345_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611704 SAMEA4791775 HS_control_21930_345_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_345 ERX2714713 E-MTAB-7037:HS_control_21930_346_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611705 SAMEA4791776 HS_control_21930_346_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_346 ERX2714714 E-MTAB-7037:HS_control_21930_347_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611706 SAMEA4791777 HS_control_21930_347_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_347 ERX2714715 E-MTAB-7037:HS_control_21930_348_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611707 SAMEA4791778 HS_control_21930_348_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_348 ERX2714716 E-MTAB-7037:HS_control_21930_349_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611708 SAMEA4791779 HS_control_21930_349_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_349 ERX2714717 E-MTAB-7037:HS_control_21930_35_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611709 SAMEA4791780 HS_control_21930_35_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_35 ERX2714718 E-MTAB-7037:HS_control_21930_350_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611710 SAMEA4791781 HS_control_21930_350_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_350 ERX2714719 E-MTAB-7037:HS_control_21930_351_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611711 SAMEA4791782 HS_control_21930_351_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_351 ERX2714720 E-MTAB-7037:HS_control_21930_352_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611712 SAMEA4791783 HS_control_21930_352_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_352 ERX2714721 E-MTAB-7037:HS_control_21930_36_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611713 SAMEA4791784 HS_control_21930_36_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_36 ERX2714722 E-MTAB-7037:HS_control_21930_363_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611714 SAMEA4791785 HS_control_21930_363_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_363 ERX2714723 E-MTAB-7037:HS_control_21930_365_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611715 SAMEA4791786 HS_control_21930_365_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_365 ERX2714724 E-MTAB-7037:HS_control_21930_366_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611716 SAMEA4791787 HS_control_21930_366_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_366 ERX2714725 E-MTAB-7037:HS_control_21930_367_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611717 SAMEA4791788 HS_control_21930_367_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_367 ERX2714726 E-MTAB-7037:HS_control_21930_368_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611718 SAMEA4791789 HS_control_21930_368_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_368 ERX2714727 E-MTAB-7037:HS_control_21930_369_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611719 SAMEA4791790 HS_control_21930_369_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_369 ERX2714728 E-MTAB-7037:HS_control_21930_37_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611720 SAMEA4791791 HS_control_21930_37_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_37 ERX2714729 E-MTAB-7037:HS_control_21930_370_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611721 SAMEA4791792 HS_control_21930_370_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_370 ERX2714730 E-MTAB-7037:HS_control_21930_371_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611722 SAMEA4791793 HS_control_21930_371_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_371 ERX2714731 E-MTAB-7037:HS_control_21930_372_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611723 SAMEA4791794 HS_control_21930_372_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_372 ERX2714732 E-MTAB-7037:HS_control_21930_373_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611724 SAMEA4791795 HS_control_21930_373_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_373 ERX2714733 E-MTAB-7037:HS_control_21930_374_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611725 SAMEA4791796 HS_control_21930_374_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_374 ERX2714734 E-MTAB-7037:HS_control_21930_375_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611726 SAMEA4791797 HS_control_21930_375_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_375 ERX2714735 E-MTAB-7037:HS_control_21930_38_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611727 SAMEA4791798 HS_control_21930_38_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_38 ERX2714736 E-MTAB-7037:HS_control_21930_39_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611728 SAMEA4791799 HS_control_21930_39_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_39 ERX2714737 E-MTAB-7037:HS_control_21930_40_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611729 SAMEA4791800 HS_control_21930_40_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_40 ERX2714738 E-MTAB-7037:HS_control_21930_42_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611730 SAMEA4791801 HS_control_21930_42_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_42 ERX2714739 E-MTAB-7037:HS_control_21930_43_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611731 SAMEA4791802 HS_control_21930_43_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_43 ERX2714740 E-MTAB-7037:HS_control_21930_44_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611732 SAMEA4791803 HS_control_21930_44_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_44 ERX2714741 E-MTAB-7037:HS_control_21930_46_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611733 SAMEA4791804 HS_control_21930_46_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_46 ERX2714742 E-MTAB-7037:HS_control_21930_51_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611734 SAMEA4791805 HS_control_21930_51_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_51 ERX2714743 E-MTAB-7037:HS_control_21930_53_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611735 SAMEA4791806 HS_control_21930_53_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_53 ERX2714744 E-MTAB-7037:HS_control_21930_55_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611736 SAMEA4791807 HS_control_21930_55_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_55 ERX2714745 E-MTAB-7037:HS_control_21930_56_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611737 SAMEA4791808 HS_control_21930_56_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_56 ERX2714746 E-MTAB-7037:HS_control_21930_57_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611738 SAMEA4791809 HS_control_21930_57_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_57 ERX2714747 E-MTAB-7037:HS_control_21930_58_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611739 SAMEA4791810 HS_control_21930_58_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_58 ERX2714748 E-MTAB-7037:HS_control_21930_59_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611740 SAMEA4791811 HS_control_21930_59_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_59 ERX2714749 E-MTAB-7037:HS_control_21930_60_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611741 SAMEA4791812 HS_control_21930_60_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_60 ERX2714750 E-MTAB-7037:HS_control_21930_61_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611742 SAMEA4791813 HS_control_21930_61_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_61 ERX2714751 E-MTAB-7037:HS_control_21930_62_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611743 SAMEA4791814 HS_control_21930_62_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_62 ERX2714752 E-MTAB-7037:HS_control_21930_63_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611744 SAMEA4791815 HS_control_21930_63_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_63 ERX2714753 E-MTAB-7037:HS_control_21930_64_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611745 SAMEA4791816 HS_control_21930_64_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_64 ERX2714754 E-MTAB-7037:HS_control_21930_65_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611746 SAMEA4791817 HS_control_21930_65_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_65 ERX2714755 E-MTAB-7037:HS_control_21930_66_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611747 SAMEA4791818 HS_control_21930_66_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_66 ERX2714756 E-MTAB-7037:HS_control_21930_68_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611748 SAMEA4791819 HS_control_21930_68_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_68 ERX2714757 E-MTAB-7037:HS_control_21930_69_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611749 SAMEA4791820 HS_control_21930_69_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_69 ERX2714758 E-MTAB-7037:HS_control_21930_7_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611750 SAMEA4791821 HS_control_21930_7_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_7 ERX2714759 E-MTAB-7037:HS_control_21930_70_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611751 SAMEA4791822 HS_control_21930_70_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_70 ERX2714760 E-MTAB-7037:HS_control_21930_71_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611752 SAMEA4791823 HS_control_21930_71_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_71 ERX2714761 E-MTAB-7037:HS_control_21930_72_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611753 SAMEA4791824 HS_control_21930_72_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_72 ERX2714762 E-MTAB-7037:HS_control_21930_76_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611754 SAMEA4791825 HS_control_21930_76_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_76 ERX2714763 E-MTAB-7037:HS_control_21930_78_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611755 SAMEA4791826 HS_control_21930_78_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_78 ERX2714764 E-MTAB-7037:HS_control_21930_79_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611756 SAMEA4791827 HS_control_21930_79_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_79 ERX2714765 E-MTAB-7037:HS_control_21930_8_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611757 SAMEA4791828 HS_control_21930_8_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_8 ERX2714766 E-MTAB-7037:HS_control_21930_80_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611758 SAMEA4791829 HS_control_21930_80_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_80 ERX2714767 E-MTAB-7037:HS_control_21930_81_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611759 SAMEA4791830 HS_control_21930_81_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_81 ERX2714768 E-MTAB-7037:HS_control_21930_82_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611760 SAMEA4791831 HS_control_21930_82_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_82 ERX2714769 E-MTAB-7037:HS_control_21930_83_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611761 SAMEA4791832 HS_control_21930_83_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_83 ERX2714770 E-MTAB-7037:HS_control_21930_84_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611762 SAMEA4791833 HS_control_21930_84_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_84 ERX2714771 E-MTAB-7037:HS_control_21930_85_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611763 SAMEA4791834 HS_control_21930_85_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_85 ERX2714772 E-MTAB-7037:HS_control_21930_86_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611764 SAMEA4791835 HS_control_21930_86_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_86 ERX2714773 E-MTAB-7037:HS_control_21930_87_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611765 SAMEA4791836 HS_control_21930_87_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_87 ERX2714774 E-MTAB-7037:HS_control_21930_88_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611766 SAMEA4791837 HS_control_21930_88_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_88 ERX2714775 E-MTAB-7037:HS_control_21930_89_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611767 SAMEA4791838 HS_control_21930_89_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_89 ERX2714776 E-MTAB-7037:HS_control_21930_9_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611768 SAMEA4791839 HS_control_21930_9_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_9 ERX2714777 E-MTAB-7037:HS_control_21930_90_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611769 SAMEA4791840 HS_control_21930_90_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_90 ERX2714778 E-MTAB-7037:HS_control_21930_91_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611770 SAMEA4791841 HS_control_21930_91_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_91 ERX2714779 E-MTAB-7037:HS_control_21930_92_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611771 SAMEA4791842 HS_control_21930_92_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_92 ERX2714780 E-MTAB-7037:HS_control_21930_93_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611772 SAMEA4791843 HS_control_21930_93_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_93 ERX2714781 E-MTAB-7037:HS_control_21930_94_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611773 SAMEA4791844 HS_control_21930_94_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_94 ERX2714782 E-MTAB-7037:HS_control_21930_95_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611774 SAMEA4791845 HS_control_21930_95_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_95 ERX2714783 E-MTAB-7037:HS_control_21930_96_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611775 SAMEA4791846 HS_control_21930_96_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_96 ERX2714784 E-MTAB-7037:HS_control_21930_99_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611776 SAMEA4791847 HS_control_21930_99_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus none Experimental Factor: time 0 Experimental Factor: single cell identifier HS_control_21930_99 ERX2714785 E-MTAB-7037:HS_pIC_2h_21920_122_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611777 SAMEA4791848 HS_pIC_2h_21920_122_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_122 ERX2714786 E-MTAB-7037:HS_pIC_2h_21920_123_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611778 SAMEA4791849 HS_pIC_2h_21920_123_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_123 ERX2714787 E-MTAB-7037:HS_pIC_2h_21920_124_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611779 SAMEA4791850 HS_pIC_2h_21920_124_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_124 ERX2714788 E-MTAB-7037:HS_pIC_2h_21920_126_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611780 SAMEA4791851 HS_pIC_2h_21920_126_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_126 ERX2714789 E-MTAB-7037:HS_pIC_2h_21920_127_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611781 SAMEA4791852 HS_pIC_2h_21920_127_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_127 ERX2714790 E-MTAB-7037:HS_pIC_2h_21920_128_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611782 SAMEA4791853 HS_pIC_2h_21920_128_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_128 ERX2714791 E-MTAB-7037:HS_pIC_2h_21920_129_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611783 SAMEA4791854 HS_pIC_2h_21920_129_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_129 ERX2714792 E-MTAB-7037:HS_pIC_2h_21920_130_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611784 SAMEA4791855 HS_pIC_2h_21920_130_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_130 ERX2714793 E-MTAB-7037:HS_pIC_2h_21920_131_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611785 SAMEA4791856 HS_pIC_2h_21920_131_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_131 ERX2714794 E-MTAB-7037:HS_pIC_2h_21920_132_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611786 SAMEA4791857 HS_pIC_2h_21920_132_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_132 ERX2714795 E-MTAB-7037:HS_pIC_2h_21920_133_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611787 SAMEA4791858 HS_pIC_2h_21920_133_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_133 ERX2714796 E-MTAB-7037:HS_pIC_2h_21920_134_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611788 SAMEA4791859 HS_pIC_2h_21920_134_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_134 ERX2714797 E-MTAB-7037:HS_pIC_2h_21920_135_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611789 SAMEA4791860 HS_pIC_2h_21920_135_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_135 ERX2714798 E-MTAB-7037:HS_pIC_2h_21920_136_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611790 SAMEA4791861 HS_pIC_2h_21920_136_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_136 ERX2714799 E-MTAB-7037:HS_pIC_2h_21920_137_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611791 SAMEA4791862 HS_pIC_2h_21920_137_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_137 ERX2714800 E-MTAB-7037:HS_pIC_2h_21920_140_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611792 SAMEA4791863 HS_pIC_2h_21920_140_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_140 ERX2714801 E-MTAB-7037:HS_pIC_2h_21920_141_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611793 SAMEA4791864 HS_pIC_2h_21920_141_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_141 ERX2714802 E-MTAB-7037:HS_pIC_2h_21920_144_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611794 SAMEA4791865 HS_pIC_2h_21920_144_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_144 ERX2714803 E-MTAB-7037:HS_pIC_2h_21920_169_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611795 SAMEA4791866 HS_pIC_2h_21920_169_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_169 ERX2714804 E-MTAB-7037:HS_pIC_2h_21920_170_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611796 SAMEA4791867 HS_pIC_2h_21920_170_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_170 ERX2714805 E-MTAB-7037:HS_pIC_2h_21920_171_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611797 SAMEA4791868 HS_pIC_2h_21920_171_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_171 ERX2714806 E-MTAB-7037:HS_pIC_2h_21920_173_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611798 SAMEA4791869 HS_pIC_2h_21920_173_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_173 ERX2714807 E-MTAB-7037:HS_pIC_2h_21920_174_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611799 SAMEA4791870 HS_pIC_2h_21920_174_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_174 ERX2714808 E-MTAB-7037:HS_pIC_2h_21920_175_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611800 SAMEA4791871 HS_pIC_2h_21920_175_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_175 ERX2714809 E-MTAB-7037:HS_pIC_2h_21920_177_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611801 SAMEA4791872 HS_pIC_2h_21920_177_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_177 ERX2714810 E-MTAB-7037:HS_pIC_2h_21920_178_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611802 SAMEA4791873 HS_pIC_2h_21920_178_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_178 ERX2714811 E-MTAB-7037:HS_pIC_2h_21920_179_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611803 SAMEA4791874 HS_pIC_2h_21920_179_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_179 ERX2714812 E-MTAB-7037:HS_pIC_2h_21920_180_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611804 SAMEA4791875 HS_pIC_2h_21920_180_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_180 ERX2714813 E-MTAB-7037:HS_pIC_2h_21920_182_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611805 SAMEA4791876 HS_pIC_2h_21920_182_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_182 ERX2714814 E-MTAB-7037:HS_pIC_2h_21920_183_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611806 SAMEA4791877 HS_pIC_2h_21920_183_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_183 ERX2714815 E-MTAB-7037:HS_pIC_2h_21920_184_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611807 SAMEA4791878 HS_pIC_2h_21920_184_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_184 ERX2714816 E-MTAB-7037:HS_pIC_2h_21920_185_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611808 SAMEA4791879 HS_pIC_2h_21920_185_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_185 ERX2714817 E-MTAB-7037:HS_pIC_2h_21920_186_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611809 SAMEA4791880 HS_pIC_2h_21920_186_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_186 ERX2714818 E-MTAB-7037:HS_pIC_2h_21920_187_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611810 SAMEA4791881 HS_pIC_2h_21920_187_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_187 ERX2714819 E-MTAB-7037:HS_pIC_2h_21920_188_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611811 SAMEA4791882 HS_pIC_2h_21920_188_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_188 ERX2714820 E-MTAB-7037:HS_pIC_2h_21920_189_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611812 SAMEA4791883 HS_pIC_2h_21920_189_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_189 ERX2714821 E-MTAB-7037:HS_pIC_2h_21920_192_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611813 SAMEA4791884 HS_pIC_2h_21920_192_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_192 ERX2714822 E-MTAB-7037:HS_pIC_2h_21920_217_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611814 SAMEA4791885 HS_pIC_2h_21920_217_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_217 ERX2714823 E-MTAB-7037:HS_pIC_2h_21920_218_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611815 SAMEA4791886 HS_pIC_2h_21920_218_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_218 ERX2714824 E-MTAB-7037:HS_pIC_2h_21920_219_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611816 SAMEA4791887 HS_pIC_2h_21920_219_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_219 ERX2714825 E-MTAB-7037:HS_pIC_2h_21920_220_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611817 SAMEA4791888 HS_pIC_2h_21920_220_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_220 ERX2714826 E-MTAB-7037:HS_pIC_2h_21920_221_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611818 SAMEA4791889 HS_pIC_2h_21920_221_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_221 ERX2714827 E-MTAB-7037:HS_pIC_2h_21920_224_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611819 SAMEA4791890 HS_pIC_2h_21920_224_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_224 ERX2714828 E-MTAB-7037:HS_pIC_2h_21920_225_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611820 SAMEA4791891 HS_pIC_2h_21920_225_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_225 ERX2714829 E-MTAB-7037:HS_pIC_2h_21920_226_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611821 SAMEA4791892 HS_pIC_2h_21920_226_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_226 ERX2714830 E-MTAB-7037:HS_pIC_2h_21920_227_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611822 SAMEA4791893 HS_pIC_2h_21920_227_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_227 ERX2714831 E-MTAB-7037:HS_pIC_2h_21920_228_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611823 SAMEA4791894 HS_pIC_2h_21920_228_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_228 ERX2714832 E-MTAB-7037:HS_pIC_2h_21920_230_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611824 SAMEA4791895 HS_pIC_2h_21920_230_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_230 ERX2714833 E-MTAB-7037:HS_pIC_2h_21920_232_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611825 SAMEA4791896 HS_pIC_2h_21920_232_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_232 ERX2714834 E-MTAB-7037:HS_pIC_2h_21920_234_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611826 SAMEA4791897 HS_pIC_2h_21920_234_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_234 ERX2714835 E-MTAB-7037:HS_pIC_2h_21920_235_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611827 SAMEA4791898 HS_pIC_2h_21920_235_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_235 ERX2714836 E-MTAB-7037:HS_pIC_2h_21920_236_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611828 SAMEA4791899 HS_pIC_2h_21920_236_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_236 ERX2714837 E-MTAB-7037:HS_pIC_2h_21920_237_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611829 SAMEA4791900 HS_pIC_2h_21920_237_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_237 ERX2714838 E-MTAB-7037:HS_pIC_2h_21920_239_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611830 SAMEA4791901 HS_pIC_2h_21920_239_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_239 ERX2714839 E-MTAB-7037:HS_pIC_2h_21920_266_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611831 SAMEA4791902 HS_pIC_2h_21920_266_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_266 ERX2714840 E-MTAB-7037:HS_pIC_2h_21920_267_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611832 SAMEA4791903 HS_pIC_2h_21920_267_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_267 ERX2714841 E-MTAB-7037:HS_pIC_2h_21920_268_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611833 SAMEA4791904 HS_pIC_2h_21920_268_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_268 ERX2714842 E-MTAB-7037:HS_pIC_2h_21920_269_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611834 SAMEA4791905 HS_pIC_2h_21920_269_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_269 ERX2714843 E-MTAB-7037:HS_pIC_2h_21920_270_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611835 SAMEA4791906 HS_pIC_2h_21920_270_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_270 ERX2714844 E-MTAB-7037:HS_pIC_2h_21920_271_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611836 SAMEA4791907 HS_pIC_2h_21920_271_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_271 ERX2714845 E-MTAB-7037:HS_pIC_2h_21920_272_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611837 SAMEA4791908 HS_pIC_2h_21920_272_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_272 ERX2714846 E-MTAB-7037:HS_pIC_2h_21920_273_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611838 SAMEA4791909 HS_pIC_2h_21920_273_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_273 ERX2714847 E-MTAB-7037:HS_pIC_2h_21920_274_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611839 SAMEA4791910 HS_pIC_2h_21920_274_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_274 ERX2714848 E-MTAB-7037:HS_pIC_2h_21920_276_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611840 SAMEA4791911 HS_pIC_2h_21920_276_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_276 ERX2714849 E-MTAB-7037:HS_pIC_2h_21920_277_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611841 SAMEA4791912 HS_pIC_2h_21920_277_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_277 ERX2714850 E-MTAB-7037:HS_pIC_2h_21920_280_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611842 SAMEA4791913 HS_pIC_2h_21920_280_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_280 ERX2714851 E-MTAB-7037:HS_pIC_2h_21920_284_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611843 SAMEA4791914 HS_pIC_2h_21920_284_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_284 ERX2714852 E-MTAB-7037:HS_pIC_2h_21920_285_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611844 SAMEA4791915 HS_pIC_2h_21920_285_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_285 ERX2714853 E-MTAB-7037:HS_pIC_2h_21920_287_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611845 SAMEA4791916 HS_pIC_2h_21920_287_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_287 ERX2714854 E-MTAB-7037:HS_pIC_2h_21920_288_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611846 SAMEA4791917 HS_pIC_2h_21920_288_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_288 ERX2714855 E-MTAB-7037:HS_pIC_2h_21920_31_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611847 SAMEA4791918 HS_pIC_2h_21920_31_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_31 ERX2714856 E-MTAB-7037:HS_pIC_2h_21920_314_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611848 SAMEA4791919 HS_pIC_2h_21920_314_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_314 ERX2714857 E-MTAB-7037:HS_pIC_2h_21920_315_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611849 SAMEA4791920 HS_pIC_2h_21920_315_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_315 ERX2714858 E-MTAB-7037:HS_pIC_2h_21920_316_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611850 SAMEA4791921 HS_pIC_2h_21920_316_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_316 ERX2714859 E-MTAB-7037:HS_pIC_2h_21920_317_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611851 SAMEA4791922 HS_pIC_2h_21920_317_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_317 ERX2714860 E-MTAB-7037:HS_pIC_2h_21920_318_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611852 SAMEA4791923 HS_pIC_2h_21920_318_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_318 ERX2714861 E-MTAB-7037:HS_pIC_2h_21920_319_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611853 SAMEA4791924 HS_pIC_2h_21920_319_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_319 ERX2714862 E-MTAB-7037:HS_pIC_2h_21920_320_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611854 SAMEA4791925 HS_pIC_2h_21920_320_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_320 ERX2714863 E-MTAB-7037:HS_pIC_2h_21920_321_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611855 SAMEA4791926 HS_pIC_2h_21920_321_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_321 ERX2714864 E-MTAB-7037:HS_pIC_2h_21920_322_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611856 SAMEA4791927 HS_pIC_2h_21920_322_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_322 ERX2714865 E-MTAB-7037:HS_pIC_2h_21920_324_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611857 SAMEA4791928 HS_pIC_2h_21920_324_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_324 ERX2714866 E-MTAB-7037:HS_pIC_2h_21920_325_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611858 SAMEA4791929 HS_pIC_2h_21920_325_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_325 ERX2714867 E-MTAB-7037:HS_pIC_2h_21920_326_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611859 SAMEA4791930 HS_pIC_2h_21920_326_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_326 ERX2714868 E-MTAB-7037:HS_pIC_2h_21920_33_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611860 SAMEA4791931 HS_pIC_2h_21920_33_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_33 ERX2714869 E-MTAB-7037:HS_pIC_2h_21920_330_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611861 SAMEA4791932 HS_pIC_2h_21920_330_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_330 ERX2714870 E-MTAB-7037:HS_pIC_2h_21920_331_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611862 SAMEA4791933 HS_pIC_2h_21920_331_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_331 ERX2714871 E-MTAB-7037:HS_pIC_2h_21920_332_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611863 SAMEA4791934 HS_pIC_2h_21920_332_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_332 ERX2714872 E-MTAB-7037:HS_pIC_2h_21920_333_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611864 SAMEA4791935 HS_pIC_2h_21920_333_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_333 ERX2714873 E-MTAB-7037:HS_pIC_2h_21920_334_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611865 SAMEA4791936 HS_pIC_2h_21920_334_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_334 ERX2714874 E-MTAB-7037:HS_pIC_2h_21920_335_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611866 SAMEA4791937 HS_pIC_2h_21920_335_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_335 ERX2714875 E-MTAB-7037:HS_pIC_2h_21920_336_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611867 SAMEA4791938 HS_pIC_2h_21920_336_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_336 ERX2714876 E-MTAB-7037:HS_pIC_2h_21920_34_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611868 SAMEA4791939 HS_pIC_2h_21920_34_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_34 ERX2714877 E-MTAB-7037:HS_pIC_2h_21920_35_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611869 SAMEA4791940 HS_pIC_2h_21920_35_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_35 ERX2714878 E-MTAB-7037:HS_pIC_2h_21920_36_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611870 SAMEA4791941 HS_pIC_2h_21920_36_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_36 ERX2714879 E-MTAB-7037:HS_pIC_2h_21920_366_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611871 SAMEA4791942 HS_pIC_2h_21920_366_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_366 ERX2714880 E-MTAB-7037:HS_pIC_2h_21920_367_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611872 SAMEA4791943 HS_pIC_2h_21920_367_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_367 ERX2714881 E-MTAB-7037:HS_pIC_2h_21920_368_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611873 SAMEA4791944 HS_pIC_2h_21920_368_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_368 ERX2714882 E-MTAB-7037:HS_pIC_2h_21920_369_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611874 SAMEA4791945 HS_pIC_2h_21920_369_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_369 ERX2714883 E-MTAB-7037:HS_pIC_2h_21920_370_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611875 SAMEA4791946 HS_pIC_2h_21920_370_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_370 ERX2714884 E-MTAB-7037:HS_pIC_2h_21920_371_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611876 SAMEA4791947 HS_pIC_2h_21920_371_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_371 ERX2714885 E-MTAB-7037:HS_pIC_2h_21920_372_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611877 SAMEA4791948 HS_pIC_2h_21920_372_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_372 ERX2714886 E-MTAB-7037:HS_pIC_2h_21920_373_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611878 SAMEA4791949 HS_pIC_2h_21920_373_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_373 ERX2714887 E-MTAB-7037:HS_pIC_2h_21920_374_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611879 SAMEA4791950 HS_pIC_2h_21920_374_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_374 ERX2714888 E-MTAB-7037:HS_pIC_2h_21920_375_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611880 SAMEA4791951 HS_pIC_2h_21920_375_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_375 ERX2714889 E-MTAB-7037:HS_pIC_2h_21920_376_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611881 SAMEA4791952 HS_pIC_2h_21920_376_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_376 ERX2714890 E-MTAB-7037:HS_pIC_2h_21920_39_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611882 SAMEA4791953 HS_pIC_2h_21920_39_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_39 ERX2714891 E-MTAB-7037:HS_pIC_2h_21920_40_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611883 SAMEA4791954 HS_pIC_2h_21920_40_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_40 ERX2714892 E-MTAB-7037:HS_pIC_2h_21920_41_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611884 SAMEA4791955 HS_pIC_2h_21920_41_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_41 ERX2714893 E-MTAB-7037:HS_pIC_2h_21920_42_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611885 SAMEA4791956 HS_pIC_2h_21920_42_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_42 ERX2714894 E-MTAB-7037:HS_pIC_2h_21920_43_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611886 SAMEA4791957 HS_pIC_2h_21920_43_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_43 ERX2714895 E-MTAB-7037:HS_pIC_2h_21920_45_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611887 SAMEA4791958 HS_pIC_2h_21920_45_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_45 ERX2714896 E-MTAB-7037:HS_pIC_2h_21920_74_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611888 SAMEA4791959 HS_pIC_2h_21920_74_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_74 ERX2714897 E-MTAB-7037:HS_pIC_2h_21920_75_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611889 SAMEA4791960 HS_pIC_2h_21920_75_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_75 ERX2714898 E-MTAB-7037:HS_pIC_2h_21920_77_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611890 SAMEA4791961 HS_pIC_2h_21920_77_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_77 ERX2714899 E-MTAB-7037:HS_pIC_2h_21920_78_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611891 SAMEA4791962 HS_pIC_2h_21920_78_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_78 ERX2714900 E-MTAB-7037:HS_pIC_2h_21920_80_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611892 SAMEA4791963 HS_pIC_2h_21920_80_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_80 ERX2714901 E-MTAB-7037:HS_pIC_2h_21920_81_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611893 SAMEA4791964 HS_pIC_2h_21920_81_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_81 ERX2714902 E-MTAB-7037:HS_pIC_2h_21920_82_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611894 SAMEA4791965 HS_pIC_2h_21920_82_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_82 ERX2714903 E-MTAB-7037:HS_pIC_2h_21920_83_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611895 SAMEA4791966 HS_pIC_2h_21920_83_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_83 ERX2714904 E-MTAB-7037:HS_pIC_2h_21920_84_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611896 SAMEA4791967 HS_pIC_2h_21920_84_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_84 ERX2714905 E-MTAB-7037:HS_pIC_2h_21920_85_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611897 SAMEA4791968 HS_pIC_2h_21920_85_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_85 ERX2714906 E-MTAB-7037:HS_pIC_2h_21920_86_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611898 SAMEA4791969 HS_pIC_2h_21920_86_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_86 ERX2714907 E-MTAB-7037:HS_pIC_2h_21920_88_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611899 SAMEA4791970 HS_pIC_2h_21920_88_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_88 ERX2714908 E-MTAB-7037:HS_pIC_2h_21920_89_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611900 SAMEA4791971 HS_pIC_2h_21920_89_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_89 ERX2714909 E-MTAB-7037:HS_pIC_2h_21920_90_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611901 SAMEA4791972 HS_pIC_2h_21920_90_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_90 ERX2714910 E-MTAB-7037:HS_pIC_2h_21920_91_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611902 SAMEA4791973 HS_pIC_2h_21920_91_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_91 ERX2714911 E-MTAB-7037:HS_pIC_2h_21920_93_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611903 SAMEA4791974 HS_pIC_2h_21920_93_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_93 ERX2714912 E-MTAB-7037:HS_pIC_2h_21920_96_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611904 SAMEA4791975 HS_pIC_2h_21920_96_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 2 Experimental Factor: single cell identifier HS_pIC_2h_21920_96 ERX2714913 E-MTAB-7037:HS_pIC_6h_21919_121_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611905 SAMEA4791976 HS_pIC_6h_21919_121_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_121 ERX2714914 E-MTAB-7037:HS_pIC_6h_21919_122_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611906 SAMEA4791977 HS_pIC_6h_21919_122_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_122 ERX2714915 E-MTAB-7037:HS_pIC_6h_21919_123_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611907 SAMEA4791978 HS_pIC_6h_21919_123_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_123 ERX2714916 E-MTAB-7037:HS_pIC_6h_21919_124_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611908 SAMEA4791979 HS_pIC_6h_21919_124_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_124 ERX2714917 E-MTAB-7037:HS_pIC_6h_21919_126_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611909 SAMEA4791980 HS_pIC_6h_21919_126_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_126 ERX2714918 E-MTAB-7037:HS_pIC_6h_21919_127_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611910 SAMEA4791981 HS_pIC_6h_21919_127_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_127 ERX2714919 E-MTAB-7037:HS_pIC_6h_21919_128_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611911 SAMEA4791982 HS_pIC_6h_21919_128_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_128 ERX2714920 E-MTAB-7037:HS_pIC_6h_21919_130_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611912 SAMEA4791983 HS_pIC_6h_21919_130_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_130 ERX2714921 E-MTAB-7037:HS_pIC_6h_21919_131_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611913 SAMEA4791984 HS_pIC_6h_21919_131_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_131 ERX2714922 E-MTAB-7037:HS_pIC_6h_21919_132_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611914 SAMEA4791985 HS_pIC_6h_21919_132_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_132 ERX2714923 E-MTAB-7037:HS_pIC_6h_21919_133_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611915 SAMEA4791986 HS_pIC_6h_21919_133_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_133 ERX2714924 E-MTAB-7037:HS_pIC_6h_21919_134_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611916 SAMEA4791987 HS_pIC_6h_21919_134_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_134 ERX2714925 E-MTAB-7037:HS_pIC_6h_21919_135_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611917 SAMEA4791988 HS_pIC_6h_21919_135_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_135 ERX2714926 E-MTAB-7037:HS_pIC_6h_21919_136_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611918 SAMEA4791989 HS_pIC_6h_21919_136_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_136 ERX2714927 E-MTAB-7037:HS_pIC_6h_21919_137_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611919 SAMEA4791990 HS_pIC_6h_21919_137_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_137 ERX2714928 E-MTAB-7037:HS_pIC_6h_21919_138_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611920 SAMEA4791991 HS_pIC_6h_21919_138_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_138 ERX2714929 E-MTAB-7037:HS_pIC_6h_21919_139_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611921 SAMEA4791992 HS_pIC_6h_21919_139_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_139 ERX2714930 E-MTAB-7037:HS_pIC_6h_21919_140_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611922 SAMEA4791993 HS_pIC_6h_21919_140_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_140 ERX2714931 E-MTAB-7037:HS_pIC_6h_21919_141_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611923 SAMEA4791994 HS_pIC_6h_21919_141_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_141 ERX2714932 E-MTAB-7037:HS_pIC_6h_21919_142_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611924 SAMEA4791995 HS_pIC_6h_21919_142_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_142 ERX2714933 E-MTAB-7037:HS_pIC_6h_21919_143_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611925 SAMEA4791996 HS_pIC_6h_21919_143_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_143 ERX2714934 E-MTAB-7037:HS_pIC_6h_21919_169_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611926 SAMEA4791997 HS_pIC_6h_21919_169_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_169 ERX2714935 E-MTAB-7037:HS_pIC_6h_21919_170_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611927 SAMEA4791998 HS_pIC_6h_21919_170_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_170 ERX2714936 E-MTAB-7037:HS_pIC_6h_21919_171_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611928 SAMEA4791999 HS_pIC_6h_21919_171_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_171 ERX2714937 E-MTAB-7037:HS_pIC_6h_21919_172_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611929 SAMEA4792000 HS_pIC_6h_21919_172_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_172 ERX2714938 E-MTAB-7037:HS_pIC_6h_21919_173_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611930 SAMEA4792001 HS_pIC_6h_21919_173_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_173 ERX2714939 E-MTAB-7037:HS_pIC_6h_21919_174_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611931 SAMEA4792002 HS_pIC_6h_21919_174_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_174 ERX2714940 E-MTAB-7037:HS_pIC_6h_21919_175_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611932 SAMEA4792003 HS_pIC_6h_21919_175_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_175 ERX2714941 E-MTAB-7037:HS_pIC_6h_21919_176_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611933 SAMEA4792004 HS_pIC_6h_21919_176_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_176 ERX2714942 E-MTAB-7037:HS_pIC_6h_21919_177_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611934 SAMEA4792005 HS_pIC_6h_21919_177_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_177 ERX2714943 E-MTAB-7037:HS_pIC_6h_21919_179_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611935 SAMEA4792006 HS_pIC_6h_21919_179_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_179 ERX2714944 E-MTAB-7037:HS_pIC_6h_21919_182_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611936 SAMEA4792007 HS_pIC_6h_21919_182_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_182 ERX2714945 E-MTAB-7037:HS_pIC_6h_21919_183_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611937 SAMEA4792008 HS_pIC_6h_21919_183_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_183 ERX2714946 E-MTAB-7037:HS_pIC_6h_21919_184_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611938 SAMEA4792009 HS_pIC_6h_21919_184_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_184 ERX2714947 E-MTAB-7037:HS_pIC_6h_21919_185_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611939 SAMEA4792010 HS_pIC_6h_21919_185_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_185 ERX2714948 E-MTAB-7037:HS_pIC_6h_21919_186_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611940 SAMEA4792011 HS_pIC_6h_21919_186_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_186 ERX2714949 E-MTAB-7037:HS_pIC_6h_21919_187_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611941 SAMEA4792012 HS_pIC_6h_21919_187_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_187 ERX2714950 E-MTAB-7037:HS_pIC_6h_21919_188_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611942 SAMEA4792013 HS_pIC_6h_21919_188_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_188 ERX2714951 E-MTAB-7037:HS_pIC_6h_21919_189_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611943 SAMEA4792014 HS_pIC_6h_21919_189_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_189 ERX2714952 E-MTAB-7037:HS_pIC_6h_21919_190_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611944 SAMEA4792015 HS_pIC_6h_21919_190_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_190 ERX2714953 E-MTAB-7037:HS_pIC_6h_21919_191_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611945 SAMEA4792016 HS_pIC_6h_21919_191_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_191 ERX2714954 E-MTAB-7037:HS_pIC_6h_21919_192_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611946 SAMEA4792017 HS_pIC_6h_21919_192_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_192 ERX2714955 E-MTAB-7037:HS_pIC_6h_21919_218_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611947 SAMEA4792018 HS_pIC_6h_21919_218_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_218 ERX2714956 E-MTAB-7037:HS_pIC_6h_21919_219_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611948 SAMEA4792019 HS_pIC_6h_21919_219_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_219 ERX2714957 E-MTAB-7037:HS_pIC_6h_21919_220_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611949 SAMEA4792020 HS_pIC_6h_21919_220_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_220 ERX2714958 E-MTAB-7037:HS_pIC_6h_21919_221_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611950 SAMEA4792021 HS_pIC_6h_21919_221_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_221 ERX2714959 E-MTAB-7037:HS_pIC_6h_21919_222_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611951 SAMEA4792022 HS_pIC_6h_21919_222_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_222 ERX2714960 E-MTAB-7037:HS_pIC_6h_21919_224_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611952 SAMEA4792023 HS_pIC_6h_21919_224_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_224 ERX2714961 E-MTAB-7037:HS_pIC_6h_21919_225_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611953 SAMEA4792024 HS_pIC_6h_21919_225_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_225 ERX2714962 E-MTAB-7037:HS_pIC_6h_21919_226_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611954 SAMEA4792025 HS_pIC_6h_21919_226_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_226 ERX2714963 E-MTAB-7037:HS_pIC_6h_21919_227_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611955 SAMEA4792026 HS_pIC_6h_21919_227_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_227 ERX2714964 E-MTAB-7037:HS_pIC_6h_21919_228_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611956 SAMEA4792027 HS_pIC_6h_21919_228_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_228 ERX2714965 E-MTAB-7037:HS_pIC_6h_21919_230_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611957 SAMEA4792028 HS_pIC_6h_21919_230_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_230 ERX2714966 E-MTAB-7037:HS_pIC_6h_21919_231_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611958 SAMEA4792029 HS_pIC_6h_21919_231_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_231 ERX2714967 E-MTAB-7037:HS_pIC_6h_21919_232_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611959 SAMEA4792030 HS_pIC_6h_21919_232_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_232 ERX2714968 E-MTAB-7037:HS_pIC_6h_21919_233_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611960 SAMEA4792031 HS_pIC_6h_21919_233_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_233 ERX2714969 E-MTAB-7037:HS_pIC_6h_21919_234_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611961 SAMEA4792032 HS_pIC_6h_21919_234_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_234 ERX2714970 E-MTAB-7037:HS_pIC_6h_21919_236_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611962 SAMEA4792033 HS_pIC_6h_21919_236_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_236 ERX2714971 E-MTAB-7037:HS_pIC_6h_21919_237_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611963 SAMEA4792034 HS_pIC_6h_21919_237_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_237 ERX2714972 E-MTAB-7037:HS_pIC_6h_21919_238_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611964 SAMEA4792035 HS_pIC_6h_21919_238_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_238 ERX2714973 E-MTAB-7037:HS_pIC_6h_21919_239_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611965 SAMEA4792036 HS_pIC_6h_21919_239_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_239 ERX2714974 E-MTAB-7037:HS_pIC_6h_21919_240_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611966 SAMEA4792037 HS_pIC_6h_21919_240_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_240 ERX2714975 E-MTAB-7037:HS_pIC_6h_21919_265_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611967 SAMEA4792038 HS_pIC_6h_21919_265_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_265 ERX2714976 E-MTAB-7037:HS_pIC_6h_21919_266_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611968 SAMEA4792039 HS_pIC_6h_21919_266_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_266 ERX2714977 E-MTAB-7037:HS_pIC_6h_21919_267_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611969 SAMEA4792040 HS_pIC_6h_21919_267_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_267 ERX2714978 E-MTAB-7037:HS_pIC_6h_21919_268_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611970 SAMEA4792041 HS_pIC_6h_21919_268_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_268 ERX2714979 E-MTAB-7037:HS_pIC_6h_21919_269_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611971 SAMEA4792042 HS_pIC_6h_21919_269_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_269 ERX2714980 E-MTAB-7037:HS_pIC_6h_21919_27_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611972 SAMEA4792043 HS_pIC_6h_21919_27_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_27 ERX2714981 E-MTAB-7037:HS_pIC_6h_21919_270_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611973 SAMEA4792044 HS_pIC_6h_21919_270_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_270 ERX2714982 E-MTAB-7037:HS_pIC_6h_21919_271_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611974 SAMEA4792045 HS_pIC_6h_21919_271_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_271 ERX2714983 E-MTAB-7037:HS_pIC_6h_21919_272_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611975 SAMEA4792046 HS_pIC_6h_21919_272_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_272 ERX2714984 E-MTAB-7037:HS_pIC_6h_21919_273_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611976 SAMEA4792047 HS_pIC_6h_21919_273_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_273 ERX2714985 E-MTAB-7037:HS_pIC_6h_21919_274_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611977 SAMEA4792048 HS_pIC_6h_21919_274_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_274 ERX2714986 E-MTAB-7037:HS_pIC_6h_21919_275_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611978 SAMEA4792049 HS_pIC_6h_21919_275_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_275 ERX2714987 E-MTAB-7037:HS_pIC_6h_21919_276_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611979 SAMEA4792050 HS_pIC_6h_21919_276_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_276 ERX2714988 E-MTAB-7037:HS_pIC_6h_21919_277_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611980 SAMEA4792051 HS_pIC_6h_21919_277_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_277 ERX2714989 E-MTAB-7037:HS_pIC_6h_21919_278_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611981 SAMEA4792052 HS_pIC_6h_21919_278_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_278 ERX2714990 E-MTAB-7037:HS_pIC_6h_21919_279_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611982 SAMEA4792053 HS_pIC_6h_21919_279_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_279 ERX2714991 E-MTAB-7037:HS_pIC_6h_21919_280_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611983 SAMEA4792054 HS_pIC_6h_21919_280_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_280 ERX2714992 E-MTAB-7037:HS_pIC_6h_21919_281_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611984 SAMEA4792055 HS_pIC_6h_21919_281_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_281 ERX2714993 E-MTAB-7037:HS_pIC_6h_21919_282_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611985 SAMEA4792056 HS_pIC_6h_21919_282_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_282 ERX2714994 E-MTAB-7037:HS_pIC_6h_21919_283_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611986 SAMEA4792057 HS_pIC_6h_21919_283_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_283 ERX2714995 E-MTAB-7037:HS_pIC_6h_21919_285_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611987 SAMEA4792058 HS_pIC_6h_21919_285_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_285 ERX2714996 E-MTAB-7037:HS_pIC_6h_21919_287_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611988 SAMEA4792059 HS_pIC_6h_21919_287_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_287 ERX2714997 E-MTAB-7037:HS_pIC_6h_21919_29_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611989 SAMEA4792060 HS_pIC_6h_21919_29_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_29 ERX2714998 E-MTAB-7037:HS_pIC_6h_21919_31_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611990 SAMEA4792061 HS_pIC_6h_21919_31_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_31 ERX2714999 E-MTAB-7037:HS_pIC_6h_21919_315_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611991 SAMEA4792062 HS_pIC_6h_21919_315_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_315 ERX2715000 E-MTAB-7037:HS_pIC_6h_21919_316_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611992 SAMEA4792063 HS_pIC_6h_21919_316_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_316 ERX2715001 E-MTAB-7037:HS_pIC_6h_21919_317_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611993 SAMEA4792064 HS_pIC_6h_21919_317_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_317 ERX2715002 E-MTAB-7037:HS_pIC_6h_21919_318_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611994 SAMEA4792065 HS_pIC_6h_21919_318_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_318 ERX2715003 E-MTAB-7037:HS_pIC_6h_21919_32_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611995 SAMEA4792066 HS_pIC_6h_21919_32_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_32 ERX2715004 E-MTAB-7037:HS_pIC_6h_21919_320_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611996 SAMEA4792067 HS_pIC_6h_21919_320_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_320 ERX2715005 E-MTAB-7037:HS_pIC_6h_21919_321_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611997 SAMEA4792068 HS_pIC_6h_21919_321_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_321 ERX2715006 E-MTAB-7037:HS_pIC_6h_21919_322_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611998 SAMEA4792069 HS_pIC_6h_21919_322_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_322 ERX2715007 E-MTAB-7037:HS_pIC_6h_21919_323_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2611999 SAMEA4792070 HS_pIC_6h_21919_323_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_323 ERX2715008 E-MTAB-7037:HS_pIC_6h_21919_324_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612000 SAMEA4792071 HS_pIC_6h_21919_324_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_324 ERX2715009 E-MTAB-7037:HS_pIC_6h_21919_325_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612001 SAMEA4792072 HS_pIC_6h_21919_325_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_325 ERX2715010 E-MTAB-7037:HS_pIC_6h_21919_326_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612002 SAMEA4792073 HS_pIC_6h_21919_326_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_326 ERX2715011 E-MTAB-7037:HS_pIC_6h_21919_327_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612003 SAMEA4792074 HS_pIC_6h_21919_327_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_327 ERX2715012 E-MTAB-7037:HS_pIC_6h_21919_328_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612004 SAMEA4792075 HS_pIC_6h_21919_328_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_328 ERX2715013 E-MTAB-7037:HS_pIC_6h_21919_329_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612005 SAMEA4792076 HS_pIC_6h_21919_329_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_329 ERX2715014 E-MTAB-7037:HS_pIC_6h_21919_33_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612006 SAMEA4792077 HS_pIC_6h_21919_33_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_33 ERX2715015 E-MTAB-7037:HS_pIC_6h_21919_330_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612007 SAMEA4792078 HS_pIC_6h_21919_330_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_330 ERX2715016 E-MTAB-7037:HS_pIC_6h_21919_331_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612008 SAMEA4792079 HS_pIC_6h_21919_331_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_331 ERX2715017 E-MTAB-7037:HS_pIC_6h_21919_332_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612009 SAMEA4792080 HS_pIC_6h_21919_332_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_332 ERX2715018 E-MTAB-7037:HS_pIC_6h_21919_333_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612010 SAMEA4792081 HS_pIC_6h_21919_333_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_333 ERX2715019 E-MTAB-7037:HS_pIC_6h_21919_334_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612011 SAMEA4792082 HS_pIC_6h_21919_334_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_334 ERX2715020 E-MTAB-7037:HS_pIC_6h_21919_335_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612012 SAMEA4792083 HS_pIC_6h_21919_335_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_335 ERX2715021 E-MTAB-7037:HS_pIC_6h_21919_34_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612013 SAMEA4792084 HS_pIC_6h_21919_34_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_34 ERX2715022 E-MTAB-7037:HS_pIC_6h_21919_35_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612014 SAMEA4792085 HS_pIC_6h_21919_35_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_35 ERX2715023 E-MTAB-7037:HS_pIC_6h_21919_36_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612015 SAMEA4792086 HS_pIC_6h_21919_36_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_36 ERX2715024 E-MTAB-7037:HS_pIC_6h_21919_363_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612016 SAMEA4792087 HS_pIC_6h_21919_363_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_363 ERX2715025 E-MTAB-7037:HS_pIC_6h_21919_364_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612017 SAMEA4792088 HS_pIC_6h_21919_364_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_364 ERX2715026 E-MTAB-7037:HS_pIC_6h_21919_365_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612018 SAMEA4792089 HS_pIC_6h_21919_365_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_365 ERX2715027 E-MTAB-7037:HS_pIC_6h_21919_366_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612019 SAMEA4792090 HS_pIC_6h_21919_366_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_366 ERX2715028 E-MTAB-7037:HS_pIC_6h_21919_367_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612020 SAMEA4792091 HS_pIC_6h_21919_367_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_367 ERX2715029 E-MTAB-7037:HS_pIC_6h_21919_368_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612021 SAMEA4792092 HS_pIC_6h_21919_368_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_368 ERX2715030 E-MTAB-7037:HS_pIC_6h_21919_369_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612022 SAMEA4792093 HS_pIC_6h_21919_369_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_369 ERX2715031 E-MTAB-7037:HS_pIC_6h_21919_37_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612023 SAMEA4792094 HS_pIC_6h_21919_37_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_37 ERX2715032 E-MTAB-7037:HS_pIC_6h_21919_372_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612024 SAMEA4792095 HS_pIC_6h_21919_372_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_372 ERX2715033 E-MTAB-7037:HS_pIC_6h_21919_373_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612025 SAMEA4792096 HS_pIC_6h_21919_373_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_373 ERX2715034 E-MTAB-7037:HS_pIC_6h_21919_374_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612026 SAMEA4792097 HS_pIC_6h_21919_374_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_374 ERX2715035 E-MTAB-7037:HS_pIC_6h_21919_375_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612027 SAMEA4792098 HS_pIC_6h_21919_375_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_375 ERX2715036 E-MTAB-7037:HS_pIC_6h_21919_376_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612028 SAMEA4792099 HS_pIC_6h_21919_376_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_376 ERX2715037 E-MTAB-7037:HS_pIC_6h_21919_38_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612029 SAMEA4792100 HS_pIC_6h_21919_38_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_38 ERX2715038 E-MTAB-7037:HS_pIC_6h_21919_383_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612030 SAMEA4792101 HS_pIC_6h_21919_383_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_383 ERX2715039 E-MTAB-7037:HS_pIC_6h_21919_40_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612031 SAMEA4792102 HS_pIC_6h_21919_40_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_40 ERX2715040 E-MTAB-7037:HS_pIC_6h_21919_41_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612032 SAMEA4792103 HS_pIC_6h_21919_41_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_41 ERX2715041 E-MTAB-7037:HS_pIC_6h_21919_42_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612033 SAMEA4792104 HS_pIC_6h_21919_42_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_42 ERX2715042 E-MTAB-7037:HS_pIC_6h_21919_43_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612034 SAMEA4792105 HS_pIC_6h_21919_43_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_43 ERX2715043 E-MTAB-7037:HS_pIC_6h_21919_44_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612035 SAMEA4792106 HS_pIC_6h_21919_44_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_44 ERX2715044 E-MTAB-7037:HS_pIC_6h_21919_45_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612036 SAMEA4792107 HS_pIC_6h_21919_45_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_45 ERX2715045 E-MTAB-7037:HS_pIC_6h_21919_46_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612037 SAMEA4792108 HS_pIC_6h_21919_46_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_46 ERX2715046 E-MTAB-7037:HS_pIC_6h_21919_73_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612038 SAMEA4792109 HS_pIC_6h_21919_73_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_73 ERX2715047 E-MTAB-7037:HS_pIC_6h_21919_74_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612039 SAMEA4792110 HS_pIC_6h_21919_74_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_74 ERX2715048 E-MTAB-7037:HS_pIC_6h_21919_75_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612040 SAMEA4792111 HS_pIC_6h_21919_75_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_75 ERX2715049 E-MTAB-7037:HS_pIC_6h_21919_76_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612041 SAMEA4792112 HS_pIC_6h_21919_76_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_76 ERX2715050 E-MTAB-7037:HS_pIC_6h_21919_77_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612042 SAMEA4792113 HS_pIC_6h_21919_77_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_77 ERX2715051 E-MTAB-7037:HS_pIC_6h_21919_78_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612043 SAMEA4792114 HS_pIC_6h_21919_78_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_78 ERX2715052 E-MTAB-7037:HS_pIC_6h_21919_79_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612044 SAMEA4792115 HS_pIC_6h_21919_79_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_79 ERX2715053 E-MTAB-7037:HS_pIC_6h_21919_80_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612045 SAMEA4792116 HS_pIC_6h_21919_80_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_80 ERX2715054 E-MTAB-7037:HS_pIC_6h_21919_81_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612046 SAMEA4792117 HS_pIC_6h_21919_81_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_81 ERX2715055 E-MTAB-7037:HS_pIC_6h_21919_82_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612047 SAMEA4792118 HS_pIC_6h_21919_82_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_82 ERX2715056 E-MTAB-7037:HS_pIC_6h_21919_83_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612048 SAMEA4792119 HS_pIC_6h_21919_83_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_83 ERX2715057 E-MTAB-7037:HS_pIC_6h_21919_84_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612049 SAMEA4792120 HS_pIC_6h_21919_84_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_84 ERX2715058 E-MTAB-7037:HS_pIC_6h_21919_85_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612050 SAMEA4792121 HS_pIC_6h_21919_85_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_85 ERX2715059 E-MTAB-7037:HS_pIC_6h_21919_86_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612051 SAMEA4792122 HS_pIC_6h_21919_86_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_86 ERX2715060 E-MTAB-7037:HS_pIC_6h_21919_87_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612052 SAMEA4792123 HS_pIC_6h_21919_87_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_87 ERX2715061 E-MTAB-7037:HS_pIC_6h_21919_88_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612053 SAMEA4792124 HS_pIC_6h_21919_88_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_88 ERX2715062 E-MTAB-7037:HS_pIC_6h_21919_89_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612054 SAMEA4792125 HS_pIC_6h_21919_89_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_89 ERX2715063 E-MTAB-7037:HS_pIC_6h_21919_90_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612055 SAMEA4792126 HS_pIC_6h_21919_90_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_90 ERX2715064 E-MTAB-7037:HS_pIC_6h_21919_91_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612056 SAMEA4792127 HS_pIC_6h_21919_91_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_91 ERX2715065 E-MTAB-7037:HS_pIC_6h_21919_92_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612057 SAMEA4792128 HS_pIC_6h_21919_92_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_92 ERX2715066 E-MTAB-7037:HS_pIC_6h_21919_93_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612058 SAMEA4792129 HS_pIC_6h_21919_93_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_93 ERX2715067 E-MTAB-7037:HS_pIC_6h_21919_94_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612059 SAMEA4792130 HS_pIC_6h_21919_94_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_94 ERX2715068 E-MTAB-7037:HS_pIC_6h_21919_95_p ERP109919 PRJEB27792 Single-cell RNA-seq of of dermal fibroblasts (supplementary - human fibroblasts stimulation with dsRNA) ERS2612060 SAMEA4792131 HS_pIC_6h_21919_95_p RNA-Seq TRANSCRIPTOMIC other Cells were washed with PBS and then trypsinized. Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). Cells were stimulated with 0.5ug/mL of High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 1uL/mL Lipofectamin 2,000, for 2 or 6 hours before collection. Sorted plates were processed according to the Smart-seq2 protocol(Picelli et al., 2014): Oligo-dT primer (IDT), dNTPs (ThermoFisher, Cat Number 10319879) and ERCC RNA Spike-In Mix (1:25,000,000 final dilution, Ambion, Cat Number 4456740) were added to each well, and Reverse Transcription (using 50U SmartScribe, Clontech Cat Number 639538) and PCR were performed following the original protocol with 25 PCR cycles. cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina, Cat Number FC-131-1096), according to the protocol supplied by Fluidigm (PN 100-5950 B1). Quality Checks on cDNA were done using a Bioanalyser 2100 (Agilent Technologies). Libraries from 96 single cells were quantified using the LightCycler 480 (Roche), pooled and purified using AMPure XP beads (Beckman Coulter) with Hamilton 384 head robot (Hamilton Robotics). Pooled samples were sequenced on an Illumina HiSeq 2500 instrument, using paired-end 125-bp reads. 200 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2500 Experimental Factor: stimulus poly(I:C) Experimental Factor: time 6 Experimental Factor: single cell identifier HS_pIC_6h_21919_95