ERS2615357 SAMEA4795433 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795433 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-1 scientific_name Homo sapiens ERS2615358 SAMEA4795434 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795434 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-3 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-3 scientific_name Homo sapiens ERS2615359 SAMEA4795435 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795435 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-4 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:HdE-CYST-Alginage-d5-HIO-4 scientific_name Homo sapiens ERS2615360 SAMEA4795436 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795436 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:HdE-CYST-Matrigel-d5-HIO-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:HdE-CYST-Matrigel-d5-HIO-1 scientific_name Homo sapiens ERS2615361 SAMEA4795437 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795437 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:HdE-CYST-Matrigel-d5-HIO-3 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:HdE-CYST-Matrigel-d5-HIO-3 scientific_name Homo sapiens ERS2615362 SAMEA4795438 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795438 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-A-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-A-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615363 SAMEA4795439 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795439 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-B-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-B-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615364 SAMEA4795440 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795440 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-C-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-C-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615365 SAMEA4795441 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795441 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-D-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-D-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615366 SAMEA4795442 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795442 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-E-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in alginate gel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Alginate-d5-tHIO-E-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615367 SAMEA4795443 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795443 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-A-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-A-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615368 SAMEA4795444 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795444 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-B-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-B-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615369 SAMEA4795445 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795445 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-D-1 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-D-1 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks ERS2615370 SAMEA4795446 9606 Homo sapiens Protocols: Human derived epithelial organoids (HdEs) or transplanted human derived epithelial organoids (tHdEs)were collected as pools from individual wells of a 24-well plate and seperated manually from the culture matrix prior to RNA extraction. Generation of HdEs and tHdEs from HIOs and tHIOs was performed using previously described methods to isolate the epithelium (Finkbeiner et al., 2015b; Hill et al., 2017; Tsai et al., 2018). In summary, HIOs and tHIOs were incubated in dispase (07923; STEMCELL Technologies) for 30 minutes on ice. Following incubation, dispase was removed and replaced with 100% fetal bovine serum for 15 minutes on ice. To mechanically separate the epithelium from mesenchyme, a volume of advanced Dulbecco's modified Eagle medium/F12 (12634010; Gibco) equal to the initial volume of fetal bovine serum was added to the tissue before vigorously pipetting the mixture several times. Epithelial fragments then settled to the bottom where they were collected manually on a stereoscope by pipet. The epithelium was washed with ice-cold advanced Dulbecco's modified Eagle medium/F12 and allowed to settle to the bottom of a 1.5-mL Eppendorf tube. The media was then withdrawn from the loose tissue pellet and replaced with Matrigel on ice. The Matrigel containing the isolated epithelium was gently mixed to evenly suspend the cells before being pipetted into individual 50-μL droplets in a 24-well plate. The plate containing the droplets was incubated at 37°C for 15 minutes to allow the Matrigel to solidify before adding LWRN growth media containing Thiazovivin (2.5 μmol/L), SB431542 (100 nmol/L), CHIR99021 (4 μmol/L), and Y27632 (10 μmol/L). After 24 hours, the media was replaced with LWRN growth media containing TZV (2.5 μmol/L), SB431542 (100 nmol/L), and CHIR99021 (4 μmol/L). After 3 days, cultures were maintained with LWRN growth media replaced every other day. Prior to transplantation, human intestinal organoids (HIOs) were mechanically dissociated from either alginate or Matrigel. HIOs were implanted under the kidney capsules of immunocompromised NOD-scid IL2Rg-null (NSG) mice (Jackson Laboratory strain no. 0005557) as previously described (Finkbeiner et al., 2015b). In summary, mice were anaesthetized using 2% isoflurane. A left-flank incision was used to expose the kidney after shaving and sterilization with isopropyl alcohol. HIOs cultured in alginate and Matrigel were surgically implanted beneath mouse kidney capsules using forceps. Mice were euthanized for retrieval of transplanted HIOs (tHIOs) after 10 weeks. RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems) RNA isolation kits and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). ENA-FIRST-PUBLIC 2019-06-27T04:02:30Z ENA-LAST-UPDATE 2018-07-17T16:15:25Z External Id SAMEA4795446 INSDC center name University of Michigan Medical School: Department of Internal Medicine, Division of Gastroenterology INSDC first public 2019-06-27T04:02:30Z INSDC last update 2018-07-17T16:15:25Z INSDC status public Submitter Id E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-E-2 broker name ArrayExpress cell line H9 cell type intestinal epithelial cell common name human developmental stage embryo genotype wild type genotype growth condition organoid culture in matrigel progenitor cell type embryonic stem cell sample name E-MTAB-7000:tHdE-CYST-Matrigel-d5-tHIO-E-2 scientific_name Homo sapiens xenograft xenografted under NOD-scid/IL2Rg-null mouse kidney capsule for 10 weeks