ERS2645464 SAMEA4825580 10090 Mus musculus Protocols: Sertoli cells were isolated from testes of 6 dpp mice and maintained in DMEM/F12 media supplemented with 2.5%FBS, 5%horse serum, 2mML-Gln, 100U/mLpenicillin, 1uMsodium pyruvate Sertoli cells lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100-300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-Androgen Receptor antibody (06-680, Millipore). ENA-FIRST-PUBLIC 2018-08-20T17:02:03Z ENA-LAST-UPDATE 2018-08-10T10:27:52Z External Id SAMEA4825580 INSDC center name School of biomedical engineering,Shanghai Jiao Tong University INSDC first public 2018-08-20T17:02:03Z INSDC last update 2018-08-10T10:27:52Z INSDC status public Submitter Id E-MTAB-7129:ChIP-seq-androgen-SSC age 6 broker name ArrayExpress cell type Sertoli cell common name house mouse genotype wild type genotype organism part testis sample name E-MTAB-7129:ChIP-seq-androgen-SSC scientific_name Mus musculus sex male strain SSC ERS2645465 SAMEA4825581 10090 Mus musculus Protocols: Sertoli cells were isolated from testes of 6 dpp mice and maintained in DMEM/F12 media supplemented with 2.5%FBS, 5%horse serum, 2mML-Gln, 100U/mLpenicillin, 1uMsodium pyruvate Sertoli cells lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100-300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-Androgen Receptor antibody (06-680, Millipore). ENA-FIRST-PUBLIC 2018-08-20T17:02:03Z ENA-LAST-UPDATE 2018-08-10T10:27:52Z External Id SAMEA4825581 INSDC center name School of biomedical engineering,Shanghai Jiao Tong University INSDC first public 2018-08-20T17:02:03Z INSDC last update 2018-08-10T10:27:52Z INSDC status public Submitter Id E-MTAB-7129:ChIP-seq-IgG-SSC-input age 6 broker name ArrayExpress cell type Sertoli cell common name house mouse genotype wild type genotype organism part testis sample name E-MTAB-7129:ChIP-seq-IgG-SSC-input scientific_name Mus musculus sex male strain SSC ERS2645466 SAMEA4825582 10090 Mus musculus Protocols: Sertoli cells were isolated from testes of 6 dpp mice and maintained in DMEM/F12 media supplemented with 2.5%FBS, 5%horse serum, 2mML-Gln, 100U/mLpenicillin, 1uMsodium pyruvate Sertoli cells lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100-300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-Androgen Receptor antibody (06-680, Millipore). ENA-FIRST-PUBLIC 2018-08-20T17:02:03Z ENA-LAST-UPDATE 2018-08-10T10:27:52Z External Id SAMEA4825582 INSDC center name School of biomedical engineering,Shanghai Jiao Tong University INSDC first public 2018-08-20T17:02:03Z INSDC last update 2018-08-10T10:27:52Z INSDC status public Submitter Id E-MTAB-7129:ChIP-seq-wt1-SSC age 6 broker name ArrayExpress cell type Sertoli cell common name house mouse genotype wild type genotype organism part testis sample name E-MTAB-7129:ChIP-seq-wt1-SSC scientific_name Mus musculus sex male strain SSC