ERS172621 SAMEA1557279 9606 Homo sapiens Protocols: HeLa cells were obtained from ECACC and grown in SILAC labelled DMEM with 10% v/v SILAC dialysed Foetal Calf Serum (Dundee Cell Products) for at least 5 population doublings. Cells were infected with wild type adenovirus serotype 5 at a multiplicity of infection of 30 Cells were re-suspended in 0.5ml 0.1% Triton X-100 to lyse the cytoplasm, nuclei were spun down and the cytoplasmic fraction was extracted with Trizol to obtain a total cytoplasmic RNA. Trizol extracted RNA was extracted again using RNAeasy (Qiagen) ENA-FIRST-PUBLIC 2012-10-27T17:01:55Z ENA-LAST-UPDATE 2018-03-08T15:58:00Z External Id SAMEA1557279 INSDC center name School of Biological Sciences, University of Bristol INSDC first public 2012-10-27T17:01:55Z INSDC last update 2018-03-08T15:58:00Z INSDC status public Submitter Id E-MTAB-1277:24HoursRead biosourceprovider ECACC broker name ArrayExpress cell line HeLa common name human disease state adenovirus infected individual 24HoursRead sample name E-MTAB-1277:24HoursRead scientific_name Homo sapiens ERS172622 SAMEA1557280 9606 Homo sapiens Protocols: HeLa cells were obtained from ECACC and grown in SILAC labelled DMEM with 10% v/v SILAC dialysed Foetal Calf Serum (Dundee Cell Products) for at least 5 population doublings. Cells were infected with wild type adenovirus serotype 5 at a multiplicity of infection of 30 Cells were re-suspended in 0.5ml 0.1% Triton X-100 to lyse the cytoplasm, nuclei were spun down and the cytoplasmic fraction was extracted with Trizol to obtain a total cytoplasmic RNA. Trizol extracted RNA was extracted again using RNAeasy (Qiagen) ENA-FIRST-PUBLIC 2012-10-27T17:01:55Z ENA-LAST-UPDATE 2018-03-08T15:58:00Z External Id SAMEA1557280 INSDC center name School of Biological Sciences, University of Bristol INSDC first public 2012-10-27T17:01:55Z INSDC last update 2018-03-08T15:58:00Z INSDC status public Submitter Id E-MTAB-1277:ZeroHoursRead biosourceprovider ECACC broker name ArrayExpress cell line HeLa common name human disease state adenovirus infected individual ZeroHoursRead sample name E-MTAB-1277:ZeroHoursRead scientific_name Homo sapiens ERS172623 SAMEA1557278 9606 Homo sapiens Protocols: HeLa cells were obtained from ECACC and grown in SILAC labelled DMEM with 10% v/v SILAC dialysed Foetal Calf Serum (Dundee Cell Products) for at least 5 population doublings. Cells were infected with wild type adenovirus serotype 5 at a multiplicity of infection of 30 Cells were re-suspended in 0.5ml 0.1% Triton X-100 to lyse the cytoplasm, nuclei were spun down and the cytoplasmic fraction was extracted with Trizol to obtain a total cytoplasmic RNA. Trizol extracted RNA was extracted again using RNAeasy (Qiagen) ENA-FIRST-PUBLIC 2012-10-27T17:01:55Z ENA-LAST-UPDATE 2018-03-08T15:58:00Z External Id SAMEA1557278 INSDC center name School of Biological Sciences, University of Bristol INSDC first public 2012-10-27T17:01:55Z INSDC last update 2018-03-08T15:58:00Z INSDC status public Submitter Id E-MTAB-1277:NineHoursRead biosourceprovider ECACC broker name ArrayExpress cell line HeLa common name human disease state adenovirus infected individual NineHoursRead sample name E-MTAB-1277:NineHoursRead scientific_name Homo sapiens