ERS12375712 SAMEA110278098 9606 Homo sapiens Protocols: Half a million cells were plated on six well plates. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xPBS before being stored as dry pellets at -80 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific). ENA-FIRST-PUBLIC 2023-06-28T00:17:09Z ENA-LAST-UPDATE 2023-06-28T00:17:09Z External Id SAMEA110278098 INSDC center alias European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC center name European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC first public 2023-06-28T00:17:09Z INSDC last update 2023-06-28T00:17:09Z INSDC status public Submitter Id E-MTAB-11914:DAOY KO empty vector broker name ArrayExpress cell_line Daoy common name human developmental stage juvenile stage disease desmoplastic/nodular medulloblastoma genotype VAPB -/- isolate not applicable organism part posterior cranial fossa sample name E-MTAB-11914:DAOY KO empty vector scientific_name Homo sapiens ERS12375713 SAMEA110278099 9606 Homo sapiens Protocols: Half a million cells were plated on six well plates. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xPBS before being stored as dry pellets at -80 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific). ENA-FIRST-PUBLIC 2023-06-28T00:17:09Z ENA-LAST-UPDATE 2023-06-28T00:17:09Z External Id SAMEA110278099 INSDC center alias European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC center name European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC first public 2023-06-28T00:17:09Z INSDC last update 2023-06-28T00:17:09Z INSDC status public Submitter Id E-MTAB-11914:USP13 KO empty vector broker name ArrayExpress cell_line USP-13-Med common name human developmental stage juvenile stage disease medulloblastoma genotype VAPB -/- isolate not applicable organism part mixed brain and cerebellum sample name E-MTAB-11914:USP13 KO empty vector scientific_name Homo sapiens ERS12375714 SAMEA110278100 9606 Homo sapiens Protocols: Half a million cells were plated on six well plates. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xPBS before being stored as dry pellets at -80 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific). ENA-FIRST-PUBLIC 2023-06-28T00:17:09Z ENA-LAST-UPDATE 2023-06-28T00:17:09Z External Id SAMEA110278100 INSDC center alias European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC center name European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC first public 2023-06-28T00:17:09Z INSDC last update 2023-06-28T00:17:09Z INSDC status public Submitter Id E-MTAB-11914:DAOY wt empty vector broker name ArrayExpress cell_line Daoy common name human developmental stage juvenile stage disease desmoplastic/nodular medulloblastoma genotype wild type genotype isolate not applicable organism part posterior cranial fossa sample name E-MTAB-11914:DAOY wt empty vector scientific_name Homo sapiens ERS12375715 SAMEA110278101 9606 Homo sapiens Protocols: Half a million cells were plated on six well plates. After 48 hours, cells were harvested using Tryple. Pelleted cells were washed with 1xPBS before being stored as dry pellets at -80 degree Celsius. For whole transcriptome analysis, RNA was isolated using the RNeasy mini kit (Qiagen) in accordance to manufacturer's protocol and RNA quality was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies). 250-500 ng of total RNA was used as input for mRNA enrichment using NEXTflex Poly(A) Beads (Bio Scientific) followed by library preparation using a NEXTflex Rapid Directional qRNA-Seq Kit (Bio Scientific). ENA-FIRST-PUBLIC 2023-06-28T00:17:09Z ENA-LAST-UPDATE 2023-06-28T00:17:09Z External Id SAMEA110278101 INSDC center alias European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC center name European Research Institute for the Biology of Ageing University Medical Center Groningen INSDC first public 2023-06-28T00:17:09Z INSDC last update 2023-06-28T00:17:09Z INSDC status public Submitter Id E-MTAB-11914:USP13 wt empty vector broker name ArrayExpress cell_line USP-13-Med common name human developmental stage juvenile stage disease medulloblastoma genotype wild type genotype isolate not applicable organism part mixed brain and cerebellum sample name E-MTAB-11914:USP13 wt empty vector scientific_name Homo sapiens