<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT accession="ERX2932570" alias="E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep1_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932570</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep1_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep1_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912814">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912814</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128429</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_treated_rep1_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: dose</TAG>
        <VALUE>10</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>sodium crotonate</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT accession="ERX2932571" alias="E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932571</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep2_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912815">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912815</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128430</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_treated_rep2_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: dose</TAG>
        <VALUE>10</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>sodium crotonate</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT accession="ERX2932572" alias="E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep3_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932572</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep3_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_treated_rep3_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912816">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912816</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128431</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_treated_rep3_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: dose</TAG>
        <VALUE>10</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>sodium crotonate</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT accession="ERX2932573" alias="E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep1_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932573</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep1_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep1_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912817">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912817</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128432</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_untreated_rep1_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>none</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT accession="ERX2932574" alias="E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep2_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932574</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep2_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep2_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912818">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912818</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128433</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_untreated_rep2_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>none</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT accession="ERX2932575" alias="E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep3_s" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX2932575</PRIMARY_ID>
      <SUBMITTER_ID namespace="LUMC">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep3_s</SUBMITTER_ID>
      <SUBMITTER_ID namespace="Leiden University Medical Center">E-MTAB-7471:HH7LVBGXY_wildtype_untreated_rep3_s</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>NextSeq 500 sequencing; RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</TITLE>
    <STUDY_REF accession="ERP112286">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP112286</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJEB29927</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>RNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptome</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS2912819">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS2912819</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA5128434</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>HH7LVBGXY_wildtype_untreated_rep3_s</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Oligo-dT</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.  Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: compound</TAG>
        <VALUE>none</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
