ERX2938575 E-MTAB-7464:1dpa-mRNA-ML-1_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922611 SAMEA5138244 1dpa-mRNA-ML-1_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 1 Experimental Factor: organism part caudal fin ERX2938576 E-MTAB-7464:1dpa-mRNA-ML-2_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922612 SAMEA5138245 1dpa-mRNA-ML-2_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 1 Experimental Factor: organism part caudal fin ERX2938577 E-MTAB-7464:1dpa-mRNA-ML-3_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922613 SAMEA5138246 1dpa-mRNA-ML-3_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 1 Experimental Factor: organism part caudal fin ERX2938578 E-MTAB-7464:1dpa-mRNA-ML-4_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922614 SAMEA5138247 1dpa-mRNA-ML-4_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 1 Experimental Factor: organism part caudal fin ERX2938579 E-MTAB-7464:7dpa-mRNA-ML-1_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922615 SAMEA5138248 7dpa-mRNA-ML-1_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 7 Experimental Factor: organism part caudal fin ERX2938580 E-MTAB-7464:7dpa-mRNA-ML-2_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922616 SAMEA5138249 7dpa-mRNA-ML-2_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 7 Experimental Factor: organism part caudal fin ERX2938581 E-MTAB-7464:7dpa-mRNA-ML-3_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922617 SAMEA5138250 7dpa-mRNA-ML-3_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 7 Experimental Factor: organism part caudal fin ERX2938582 E-MTAB-7464:7dpa-mRNA-ML-4_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922618 SAMEA5138251 7dpa-mRNA-ML-4_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 7 Experimental Factor: organism part caudal fin ERX2938583 E-MTAB-7464:Cnt-mRNA-ML-1_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922619 SAMEA5138252 Cnt-mRNA-ML-1_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 0 Experimental Factor: organism part caudal fin ERX2938584 E-MTAB-7464:Cnt-mRNA-ML-2_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922620 SAMEA5138253 Cnt-mRNA-ML-2_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 0 Experimental Factor: organism part caudal fin ERX2938585 E-MTAB-7464:Cnt-mRNA-ML-3_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922621 SAMEA5138254 Cnt-mRNA-ML-3_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 0 Experimental Factor: organism part caudal fin ERX2938586 E-MTAB-7464:Cnt-mRNA-ML-4_p ERP112367 PRJEB30004 RNA-seq to investigate the genetic pathways in brain and melanocyte regeneration using a zebrafish model ERS2922622 SAMEA5138255 Cnt-mRNA-ML-4_p ssRNA-seq TRANSCRIPTOMIC other For stab wound approach, firstly, fish were anesthetized by using Tricaine (Sigma). A 30 gauge syringe needle was pushed through a nostril along the rostrocaudal body axis until the end of the one hemisphere of telencephalon. Then they were allowed for recovery of the wound in fresh water. At 3dpl and 14dpl (day post lesion) their stab lesioned hemispheres were dissected as early and late stage, respectively. They were put into RNAlater solution immediately to prevent tissue degradation. For melanocyte regeneration, Zebrafish melanocytes were chemically ablated by one-day NCP treatment. NCP was washed after 24 hours of treatment. NCP (Sigma-Aldrich) was prepared as 100 mM stock solutions in DMSO. Final concentration of NCP was used as 100 µM for each group. The early and late stages of melanocyte regeneration were determined as 1 day after ablation (dpa) and 7 days after ablation, respectively. Firstly, fish were anesthetized by Tricaine. Once fish were immobilized, the caudal fin of each fish were resected, and fins were placed into QIAzol Lysis Reagent and immediately proceeded to tissue disruption and homogenization. After homogenization of all brain and melanocyte tissues, their RNAs were isolated by RNeasy® Micro Kit (QIAGEN). The RNA quality and integrity were checked by the Agilent 2100 Bioanalyzer System. Adult zebrafish were fed under normal conditions 1 micromolar Neocuproine for some samples RNA-seq libraries were built using the TruSeq RNA Library Preparation Kit from Illumina 160 0 F Application Read Forward 1 1 R Application Read Reverse 81 NextSeq 500 Experimental Factor: compound neocuproine Experimental Factor: dose 1 Experimental Factor: injury chemical ablation Experimental Factor: time 0 Experimental Factor: organism part caudal fin