ERP113090 PRJEB30615 ena-STUDY-Furtwangen University-08-01-2019-09:56:29:391-647 Identification of Intestinal Bacterial Taxa with a Potential Role in Parkinson's Disease using Illumina MiSeq and Ion Torrent Next Generation Sequencing Parkinson's disease (PD) is one of the most common neurodegenerative disorders. It has been repeatedly reported that most patients with PD suffer from gastrointestinal dysfunctions and alterations of the autonomous nervous system in the gut wall called the enteric nervous system (ENS). Such alterations and functional gastrointestinal deficits might occur years before the classical clinical symptoms of PD appear. Until now, only little is known about PD-associated changes in gut microbiota composition and their implication in PD development. Due to variance in previously published reports about the differences between community composition in PD and non-PD patients, our intention is to increase knowledge in this field with the use of two next generation sequencing (NGS) methods (Illumina MiSeq and Ion Torrent PGM) on the same set of samples. The V4 and V5 hypervariable region of bacterial 16S rRNA genes was PCR-amplified from stool samples of 39 PD patients and 25 healthy, age-matched control persons, respectively. Illumina- and Ion Torrent- based sequencing yielded divergent as well as congruent results regarding the bacterial microbiota composition of PD and control samples.Bacterial taxa with differences in relative abundance between PD and control samples that were traceable with both sequencing techniques might play a role for supporting PD diagnosis and therapy. Intestinal Bacterial Taxa in Parkinson's Disease Parkinson's disease (PD) is one of the most common neurodegenerative disorders. It has been repeatedly reported that most patients with PD suffer from gastrointestinal dysfunctions and alterations of the autonomous nervous system in the gut wall called the enteric nervous system (ENS). Such alterations and functional gastrointestinal deficits might occur years before the classical clinical symptoms of PD appear. Until now, only little is known about PD-associated changes in gut microbiota composition and their implication in PD development. Due to variance in previously published reports about the differences between community composition in PD and non-PD patients, our intention is to increase knowledge in this field with the use of two next generation sequencing (NGS) methods (Illumina MiSeq and Ion Torrent PGM) on the same set of samples. The V4 and V5 hypervariable region of bacterial 16S rRNA genes was PCR-amplified from stool samples of 39 PD patients and 25 healthy, age-matched control persons, respectively. Illumina- and Ion Torrent- based sequencing yielded divergent as well as congruent results regarding the bacterial microbiota composition of PD and control samples.Bacterial taxa with differences in relative abundance between PD and control samples that were traceable with both sequencing techniques might play a role for supporting PD diagnosis and therapy. ENA-FIRST-PUBLIC 2019-12-11 ENA-LAST-UPDATE 2019-01-08