ERS3122264 SAMEA5315023 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315023 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 2 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition control organism part umbilical vein sample name E-MTAB-7647:Sample 2 scientific_name Homo sapiens ERS3122265 SAMEA5315024 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315024 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 3 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition control organism part umbilical vein sample name E-MTAB-7647:Sample 3 scientific_name Homo sapiens ERS3122266 SAMEA5315025 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315025 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 4 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition control organism part umbilical vein sample name E-MTAB-7647:Sample 4 scientific_name Homo sapiens ERS3122267 SAMEA5315026 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315026 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 5 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition high LDL organism part umbilical vein sample name E-MTAB-7647:Sample 5 scientific_name Homo sapiens ERS3122268 SAMEA5315027 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315027 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 6 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition high LDL organism part umbilical vein sample name E-MTAB-7647:Sample 6 scientific_name Homo sapiens ERS3122269 SAMEA5315028 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315028 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 7 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition high LDL organism part umbilical vein sample name E-MTAB-7647:Sample 7 scientific_name Homo sapiens ERS3122270 SAMEA5315029 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315029 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 8 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition high LDL organism part umbilical vein sample name E-MTAB-7647:Sample 8 scientific_name Homo sapiens ERS3122263 SAMEA5315022 9606 Homo sapiens Protocols: Human umbilical vein endothelial cells (HUVEC), purchased from Lonza (Cologne, Germany), were cultured in endothelial basal growth media (EBM) from Lonza, supplemented with hydrocortisone (1 µg/ml), bovine brain extract (12 µg/ml), gentamicin (50 µg/ml) human epidermal growth factor (10 ng/ml) and 10 % fetal calf serum. To simulate an unhealthy diet cells were seeded into cell culture dishes and incubated with the cultivation media for two days. Subsequently cells were treated with media, which was supplemented with either 0.4 mg/ml fructose (con) or 0.4 mg/ml fructose and 1 mg/ml LDL (high LDL). This media was refreshed every second to third day up for 5 to 7 days depending on the experiment. Total RNA-Isolation was performed by using the RNeasy kit from QIAGEN (Hilden, Germany). Cells were lysed using trizol and total RNA was precipitated by adding isopropanol. After centrifugation of this mixture, the liquid phase (RNA) was transferred to a QIAspin column. Subsequently RNA was washed and eluted following the manufacture guidelines. The RNA concentration was measured using a NanoDrop 2000c from Thermo Fisher (Schwerte, Germany) and RNA integrity as well as purity was analyzed by agarose gel electrophoresis. 1. Normalization: Dilution to 200 ng in 50 μL 2. Library Preparation: 50 μL of total RNA sample input. The Library preparation was performed according to the TruSeq® Stranded mRNA Sample Preparation Guide (Part # 15031047 Rev. E) on Sciclone NGS roboter (PerkinElmer). ENA-FIRST-PUBLIC 2019-02-26T17:02:06Z ENA-LAST-UPDATE 2019-02-01T13:36:43Z External Id SAMEA5315022 INSDC center name Heinrich-Heine University Duesseldorf INSDC first public 2019-02-26T17:02:06Z INSDC last update 2019-02-01T13:36:43Z INSDC status public Submitter Id E-MTAB-7647:Sample 1 broker name ArrayExpress cell line HUVEC cell type endothelial cell common name human growth condition control organism part umbilical vein sample name E-MTAB-7647:Sample 1 scientific_name Homo sapiens