ERP114200 PRJEB31625 ena-STUDY-BEIJING FORESTRY UNIVERSITY-10-03-2019-04:35:42:387-428 By regular analyses and high-throughput sequencing (Illumina Miseq), we study the effects of three year N (0, 7.5, 15 g N m-2 year-1) and P (0, 7.5, 15 and 30 g P2O5 m-2 year-1) addition on the soil chemical properties, AMF colonization, OTU richness, diversity index and community composition. ?Objective?To investigate the responses of arbuscular mycorrhizal fungi (AMF) community to nitrogen (N) and phosphorus (P) in the middle-high altitude area of Qinghai-Tibetan alpine meadow, and have a better knowledge of the effects of fertilization on alpine meadow AMF community.?Method?By regular analyses and high-throughput sequencing (Illumina Miseq), we study the effects of three year N (0, 7.5, 15 g N m-2 year-1) and P (0, 7.5, 15 and 30 g P2O5 m-2 year-1) addition on the soil chemical properties, AMF colonization, OTU richness, diversity index and community composition.?Result?36 AMF OTU from seven families were detected in this study. The addition of N, P and their interaction effect has no influence on AMF colonization, OTU richness and diversity index. Compared to light N addition treatments, the high N input treatment decrease the relative abundance of Glomeraceae. SOC, NO-N, AM and TP are the factors which impacted the AMF community.?Conclusion?The responses of AMF community to N and P addition in middle-high altitude alpine meadow are different from those in low altitude alpine meadow in Qinghai-Tibetan plateau. Further studies need to conduct in higher altitude alpine meadow and start the researches on AMF function. Effects of N and P Addition to the Arbuscular Mycorrhizal Fungal Community in the Middle-High Altitude Alpine Meadow in Qinghai-Tibetan Plateau ?Objective?To investigate the responses of arbuscular mycorrhizal fungi (AMF) community to nitrogen (N) and phosphorus (P) in the middle-high altitude area of Qinghai-Tibetan alpine meadow, and have a better knowledge of the effects of fertilization on alpine meadow AMF community.?Method?By regular analyses and high-throughput sequencing (Illumina Miseq), we study the effects of three year N (0, 7.5, 15 g N m-2 year-1) and P (0, 7.5, 15 and 30 g P2O5 m-2 year-1) addition on the soil chemical properties, AMF colonization, OTU richness, diversity index and community composition.?Result?36 AMF OTU from seven families were detected in this study. The addition of N, P and their interaction effect has no influence on AMF colonization, OTU richness and diversity index. Compared to light N addition treatments, the high N input treatment decrease the relative abundance of Glomeraceae. SOC, NO-N, AM and TP are the factors which impacted the AMF community.?Conclusion?The responses of AMF community to N and P addition in middle-high altitude alpine meadow are different from those in low altitude alpine meadow in Qinghai-Tibetan plateau. Further studies need to conduct in higher altitude alpine meadow and start the researches on AMF function. ENA-FIRST-PUBLIC 2019-03-10 ENA-LAST-UPDATE 2019-03-10