ERX3252098 E-MTAB-7786:SW837 H2Bub1 1_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230396 SAMEA5425107 SW837 H2Bub1 1_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H2Bub1 ERX3252099 E-MTAB-7786:SW837 H2Bub1 2_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230397 SAMEA5425108 SW837 H2Bub1 2_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H2Bub1 ERX3252100 E-MTAB-7786:SW837 H2Bub1 3_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230398 SAMEA5425109 SW837 H2Bub1 3_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H2Bub1 ERX3252101 E-MTAB-7786:SW837 H3K27ac 1_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230399 SAMEA5425110 SW837 H3K27ac 1_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K27ac ERX3252102 E-MTAB-7786:SW837 H3K27ac 2_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230400 SAMEA5425111 SW837 H3K27ac 2_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K27ac ERX3252103 E-MTAB-7786:SW837 H3K27ac 3_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230401 SAMEA5425112 SW837 H3K27ac 3_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K27ac ERX3252104 E-MTAB-7786:SW837 H3K79me3 1_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230402 SAMEA5425113 SW837 H3K79me3 1_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K79me3 ERX3252105 E-MTAB-7786:SW837 H3K79me3 2_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230403 SAMEA5425114 SW837 H3K79me3 2_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K79me3 ERX3252106 E-MTAB-7786:SW837 H3K79me3 3_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230404 SAMEA5425115 SW837 H3K79me3 3_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate H3K79me3 ERX3252107 E-MTAB-7786:SW837 Input 1_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230405 SAMEA5425116 SW837 Input 1_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate input DNA ERX3252108 E-MTAB-7786:SW837 Input 2_s ERP114327 PRJEB31734 ChIP-seq for H2Bub1, H3K27ac and H3K79me3 in SW837 cells ERS3230406 SAMEA5425117 SW837 Input 2_s ChIP-Seq GENOMIC ChIP SW837 cells were grown in DMEM/F-12 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. Upon washing with PBS, cells were crosslinked with 1% formaldehyde for 20 minutes and quenched using 125 mM glycine. Upon scraping, nuclear pellets were prepared in and washed with the nuclear preparation buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 7.5), 0.5% v/v NP-40, 1% v/v Triton-X-100, 20 mM NaF). Samples were sonicated in sonication buffer (150mM NaCl, 20 mM EDTA, 50 mM Tris-HCl (pH 8), 1% v/v NP-40, 0.5% v/v Sodium deoxycholate, 20 mM NaF, 0.1% SDS) for 20 cycles (30s on/off). Samples were precleared using 50% Sepharose 4B slurry (GE Healthcare) and incubated with the respective antibodies overnight: H3K27ac (196-050, Diagenode), H3K79me3 (C15310068, Diagenode) and H2Bub1 (55465, Cell Signaling). Sepharose beads with Protein A (for rabbit antibodies) or Protein G (for mouse antibodies) were added to samples and incubated for 2 hours followed by washes, de-crosslinking, and DNA extraction. DNA extraction was performed using Roti(R)-phenol/chloroform/Isoamylalcohol (Roth) followed by precipitation in 100% ethanol and washes with 70% ethanol. Libraries for DNA from ChIP were made using the NEBNext Ultra DNA library preparation kit according to the manufacturer's instructions. Illumina HiSeq 2000 Experimental Factor: immunoprecipitate input DNA