ERS3325564 SAMEA5523481 10090 Mus musculus Protocols: ChIP samples were pre-cleared and then incubated with GFP-Trap MA at 4 degrees overnight. Precipitates were then washed to remove non-specifically bound proteins and DNA 5 x 10^7 CD4 T cells from GFP-Egr2 mice were stimulated with anti-CD3 and anti-CD28 for 24 before harvesting Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and the reaction was then quenched with 125mM Glycine Chromatin was sheared by sonication with a Bioruptor Pico (Diagenode) Crosslinks were reversed and protein degraded by heating to 65C in the presence of Proteinase K. DNA was then purified by phenol chloroform extraction and ethanol precipitation The libraries were generated from the DNA using the NEBNext Ultra II DNA library Prep kit (New England Biolabs) according to the manufacturer's instructions ENA-FIRST-PUBLIC 2019-07-31T04:02:46Z ENA-LAST-UPDATE 2019-03-21T08:56:19Z External Id SAMEA5523481 INSDC center name The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London INSDC first public 2019-07-31T04:02:46Z INSDC last update 2019-03-21T08:56:19Z INSDC status public Submitter Id E-MTAB-7797:GFP_Trap_1 age 7 broker name ArrayExpress common name house mouse developmental stage adult genotype Egr2_Kin individual Mixed pool from 10 mice organism part Lymph node and spleen sample name E-MTAB-7797:GFP_Trap_1 scientific_name Mus musculus sex mixed strain C57BL/6 ERS3325565 SAMEA5523482 10090 Mus musculus Protocols: ChIP samples were pre-cleared and then incubated with GFP-Trap MA at 4 degrees overnight. Precipitates were then washed to remove non-specifically bound proteins and DNA 5 x 10^7 CD4 T cells from GFP-Egr2 mice were stimulated with anti-CD3 and anti-CD28 for 24 before harvesting Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and the reaction was then quenched with 125mM Glycine Chromatin was sheared by sonication with a Bioruptor Pico (Diagenode) Crosslinks were reversed and protein degraded by heating to 65C in the presence of Proteinase K. DNA was then purified by phenol chloroform extraction and ethanol precipitation The libraries were generated from the DNA using the NEBNext Ultra II DNA library Prep kit (New England Biolabs) according to the manufacturer's instructions ENA-FIRST-PUBLIC 2019-07-31T04:02:46Z ENA-LAST-UPDATE 2019-03-21T08:56:19Z External Id SAMEA5523482 INSDC center name The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London INSDC first public 2019-07-31T04:02:46Z INSDC last update 2019-03-21T08:56:19Z INSDC status public Submitter Id E-MTAB-7797:GFP_Trap_2 age 7 broker name ArrayExpress common name house mouse developmental stage adult genotype Egr2_Kin individual Mixed pool from 10 mice organism part Lymph node and spleen sample name E-MTAB-7797:GFP_Trap_2 scientific_name Mus musculus sex mixed strain C57BL/6 ERS3325566 SAMEA5523483 10090 Mus musculus Protocols: ChIP samples were pre-cleared and then incubated with GFP-Trap MA at 4 degrees overnight. Precipitates were then washed to remove non-specifically bound proteins and DNA 5 x 10^7 CD4 T cells from GFP-Egr2 mice were stimulated with anti-CD3 and anti-CD28 for 24 before harvesting Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and the reaction was then quenched with 125mM Glycine Chromatin was sheared by sonication with a Bioruptor Pico (Diagenode) Crosslinks were reversed and protein degraded by heating to 65C in the presence of Proteinase K. DNA was then purified by phenol chloroform extraction and ethanol precipitation The libraries were generated from the DNA using the NEBNext Ultra II DNA library Prep kit (New England Biolabs) according to the manufacturer's instructions ENA-FIRST-PUBLIC 2019-07-31T04:02:46Z ENA-LAST-UPDATE 2019-03-21T08:56:19Z External Id SAMEA5523483 INSDC center name The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London INSDC first public 2019-07-31T04:02:46Z INSDC last update 2019-03-21T08:56:19Z INSDC status public Submitter Id E-MTAB-7797:GFP_Trap_3 age 7 broker name ArrayExpress common name house mouse developmental stage adult genotype Egr2_Kin individual Mixed pool from 10 mice organism part Lymph node and spleen sample name E-MTAB-7797:GFP_Trap_3 scientific_name Mus musculus sex mixed strain C57BL/6 ERS3325567 SAMEA5523484 10090 Mus musculus Protocols: ChIP samples were pre-cleared and then incubated with GFP-Trap MA at 4 degrees overnight. Precipitates were then washed to remove non-specifically bound proteins and DNA 5 x 10^7 CD4 T cells from GFP-Egr2 mice were stimulated with anti-CD3 and anti-CD28 for 24 before harvesting Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and the reaction was then quenched with 125mM Glycine Chromatin was sheared by sonication with a Bioruptor Pico (Diagenode) Crosslinks were reversed and protein degraded by heating to 65C in the presence of Proteinase K. DNA was then purified by phenol chloroform extraction and ethanol precipitation The libraries were generated from the DNA using the NEBNext Ultra II DNA library Prep kit (New England Biolabs) according to the manufacturer's instructions ENA-FIRST-PUBLIC 2019-07-31T04:02:46Z ENA-LAST-UPDATE 2019-03-21T08:56:19Z External Id SAMEA5523484 INSDC center name The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London INSDC first public 2019-07-31T04:02:46Z INSDC last update 2019-03-21T08:56:19Z INSDC status public Submitter Id E-MTAB-7797:GFP_Trap_4 age 7 broker name ArrayExpress common name house mouse developmental stage adult genotype Egr2_Kin individual Mixed pool from 10 mice organism part Lymph node and spleen sample name E-MTAB-7797:GFP_Trap_4 scientific_name Mus musculus sex mixed strain C57BL/6 ERS3325568 SAMEA5523485 10090 Mus musculus Protocols: A proportion of chromatin was saved as an Input sample 5 x 10^7 CD4 T cells from GFP-Egr2 mice were stimulated with anti-CD3 and anti-CD28 for 24 before harvesting Cells were crosslinked with 1% Formaldehyde for 10 min at room temperature and the reaction was then quenched with 125mM Glycine Chromatin was sheared by sonication with a Bioruptor Pico (Diagenode) Crosslinks were reversed and protein degraded by heating to 65C in the presence of Proteinase K. DNA was then purified by phenol chloroform extraction and ethanol precipitation The libraries were generated from the DNA using the NEBNext Ultra II DNA library Prep kit (New England Biolabs) according to the manufacturer's instructions ENA-FIRST-PUBLIC 2019-07-31T04:02:46Z ENA-LAST-UPDATE 2019-03-21T08:56:19Z External Id SAMEA5523485 INSDC center name The Blizard Institute Barts and The London School of Medicine and Dentistry, Queen Mary, University of London INSDC first public 2019-07-31T04:02:46Z INSDC last update 2019-03-21T08:56:19Z INSDC status public Submitter Id E-MTAB-7797:Input age 7 broker name ArrayExpress common name house mouse developmental stage adult genotype Egr2_Kin individual Mixed pool from 10 mice organism part Lymph node and spleen sample name E-MTAB-7797:Input scientific_name Mus musculus sex mixed strain C57BL/6