<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="E-SYBR-1:AF300-2" accession="ERX207014" broker_name="ArrayExpress">
    <IDENTIFIERS>
      <PRIMARY_ID>ERX207014</PRIMARY_ID>
      <SUBMITTER_ID namespace="EMBL EUROPEAN BIOINFORMATICS INSTITUTE">E-SYBR-1:AF300-2</SUBMITTER_ID>
      <SUBMITTER_ID namespace="EMBL European Bioinformatics Institute">E-SYBR-1:AF300-2</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Illumina HiSeq 2000 paired end sequencing; Multiazole resistance in clinical and environmental fungal isolates</TITLE>
    <STUDY_REF accession="ERP002270" refcenter="EMBL European Bioinformatics Institute" refname="E-SYBR-1">
      <IDENTIFIERS>
        <PRIMARY_ID>ERP002270</PRIMARY_ID>
        <SUBMITTER_ID namespace="EMBL European Bioinformatics Institute">E-SYBR-1</SUBMITTER_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Multiazole resistance in clinical and environmental fungal isolates</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="ERS216925" refname="E-SYBR-1:AF300-2" refcenter="EMBL European Bioinformatics Institute">
        <IDENTIFIERS>
          <PRIMARY_ID>ERS216925</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMEA2051880</EXTERNAL_ID>
          <SUBMITTER_ID namespace="EMBL European Bioinformatics Institute">E-SYBR-1:AF300-2</SUBMITTER_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>AF300-2</LIBRARY_NAME>
        <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>GENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED NOMINAL_LENGTH="450"/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Genomic DNA was extracted using the CTAB genomic DNA extraction method. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3' thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). Insert sizes were 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: strain</TAG>
        <VALUE>AF300-2</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: clinical history</TAG>
        <VALUE>clinical sample</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: pathogenicity</TAG>
        <VALUE>pathogenic</VALUE>
      </EXPERIMENT_ATTRIBUTE>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>Experimental Factor: overall azole resistance</TAG>
        <VALUE>azole-sensitive</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
