ERP007630 PRJEB1503 ena-PROJECT-CSISP-22-02-2013-20:23:09:189-5 Whole Genome Shotgun sequencing of the Streptococcus sp. str CECT 7746 isolated from human caries-free tooth surface. Two Streptococci species were isolated from tooth surface of people who never have had caries. These isolates have specific growth inhibition on the Streptococcus mutans species. With the aim to detect the specific molecular pathway to inhibit the growth of S. mutans, we decided to sequence the entire genome of these isolates. Preliminary 16S rDNA based phylogeny demonstrated close relationship with S. oralis and S. mitis species. Genome wide comparisons using Average Nucleotide Identity (ANI) has shown DNA signature from Streptococcal species but significantly differ from S. oralis and S. mitis. Therefore, this evidences that both Streptococcus sp. str CECT 7746 and CECT 7747 are new Streptococcus species with potential usage as probiotic for human oral health. Genome sequencing of the Streptococcus sp. str CECT 7746. Two Streptococci species were isolated from tooth surface of people who never have had caries. These isolates have specific growth inhibition on the Streptococcus mutans species. With the aim to detect the specific molecular pathway to inhibit the growth of S. mutans, we decided to sequence the entire genome of these isolates. Preliminary 16S rDNA based phylogeny demonstrated close relationship with S. oralis and S. mitis species. Genome wide comparisons using Average Nucleotide Identity (ANI) has shown DNA signature from Streptococcal species but significantly differ from S. oralis and S. mitis. Therefore, this evidences that both Streptococcus sp. str CECT 7746 and CECT 7747 are new Streptococcus species with potential usage as probiotic for human oral health.