ERS3496963 SAMEA5693001 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693001 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 10 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 10 organism part lung sample name E-MTAB-8031:NEC 10 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex female ERS3496964 SAMEA5693002 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693002 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 11 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 11 organism part lung sample name E-MTAB-8031:NEC 11 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex female ERS3496965 SAMEA5693003 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693003 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 14 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 14 organism part lung sample name E-MTAB-8031:NEC 14 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496966 SAMEA5693004 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693004 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 15 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 15 organism part lung sample name E-MTAB-8031:NEC 15 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496967 SAMEA5693005 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693005 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 20 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 20 organism part lung sample name E-MTAB-8031:NEC 20 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496968 SAMEA5693006 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693006 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 22 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 22 organism part lung sample name E-MTAB-8031:NEC 22 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496969 SAMEA5693007 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693007 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 25 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 25 organism part lung sample name E-MTAB-8031:NEC 25 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496970 SAMEA5693008 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693008 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 26 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 26 organism part lung sample name E-MTAB-8031:NEC 26 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496971 SAMEA5693009 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693009 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 5 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 5 organism part lung sample name E-MTAB-8031:NEC 5 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496972 SAMEA5693010 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693010 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 6 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 6 organism part lung sample name E-MTAB-8031:NEC 6 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496973 SAMEA5693011 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693011 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 8 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 8 organism part lung sample name E-MTAB-8031:NEC 8 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496974 SAMEA5693012 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693012 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:NEC 9 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 9 organism part lung sample name E-MTAB-8031:NEC 9 sampling site normal tissue adjacent to tumor scientific_name Homo sapiens sex male ERS3496975 SAMEA5693013 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693013 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 10 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 10 organism part lung sample name E-MTAB-8031:TEC 10 sampling site neoplasm scientific_name Homo sapiens sex female ERS3496976 SAMEA5693014 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693014 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 11 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 11 organism part lung sample name E-MTAB-8031:TEC 11 sampling site neoplasm scientific_name Homo sapiens sex female ERS3496977 SAMEA5693015 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693015 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 14 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 14 organism part lung sample name E-MTAB-8031:TEC 14 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496978 SAMEA5693016 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693016 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 15 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 15 organism part lung sample name E-MTAB-8031:TEC 15 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496979 SAMEA5693017 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693017 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 16 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 16 organism part lung sample name E-MTAB-8031:TEC 16 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496980 SAMEA5693018 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693018 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 20 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 20 organism part lung sample name E-MTAB-8031:TEC 20 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496981 SAMEA5693019 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693019 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 22 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 22 organism part lung sample name E-MTAB-8031:TEC 22 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496982 SAMEA5693020 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693020 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 25 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 25 organism part lung sample name E-MTAB-8031:TEC 25 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496983 SAMEA5693021 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693021 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 26 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 26 organism part lung sample name E-MTAB-8031:TEC 26 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496984 SAMEA5693022 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:13Z External Id SAMEA5693022 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:13Z INSDC status public Submitter Id E-MTAB-8031:TEC 5 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 5 organism part lung sample name E-MTAB-8031:TEC 5 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496985 SAMEA5693023 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:14Z External Id SAMEA5693023 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:14Z INSDC status public Submitter Id E-MTAB-8031:TEC 6 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 6 organism part lung sample name E-MTAB-8031:TEC 6 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496986 SAMEA5693024 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:14Z External Id SAMEA5693024 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:14Z INSDC status public Submitter Id E-MTAB-8031:TEC 8 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 8 organism part lung sample name E-MTAB-8031:TEC 8 sampling site neoplasm scientific_name Homo sapiens sex male ERS3496987 SAMEA5693025 9606 Homo sapiens Protocols: Freshly-dissected tissue was placed into a C-tube (Miltenyi Biotec) containing 5 ml digestion buffer (0.1% collagenase II (Thermo Fisher Scientific), 0.25% collagenase type 4 (Worthington Biochemical), DNase I (150 U/min) in DMEM supplemented with 1x sodium pyruvate, 1x MEM NEAAs, ECGF/Heparin, antibiotic/antimycotic (2x) and 1% penicillin/streptomycin (Thermo Fisher Scientific)) and processed with the m_lung_01 program of gentleMACS (Miltenyi Biotec). The samples were then incubated in a water bath at 37°C for 30 min with manual shaking every 5 min. At the end of digestion, the samples were processed with the m_lung_02 program of gentleMACS, and the reaction was stopped by adding 10 ml of an isolation buffer containing 1x PBS, 0.1% BSA, 5 mM EDTA. Subsequently, the cell suspension was filtered through a 100 m cell strainer (Corning) on ice. Finally, ECs were enriched by magnetic bead sorting using MACS system (Miltenyi Biotec) at RT. Immune cells were depleted using selection with CD45 MicroBeads (Miltenyi Biotec), followed by positive selection of ECs with CD31 MicroBeads (Miltenyi Biotec), according to the manufacturer's procedures. Human tumor and normal ECs were resuspended in 6 ml of a 1:1 mix of M199 (containing 10% FBS and sodium pyruvate, MEM NEAAs, MEM vitamin solution, glutamine and heparin as above) and EGM2 medium, further supplemented with antibiotic/antimycotic (2x) (Thermo Fisher Scientific), and the single cell suspension was plated out in 3 wells of a 6-well plate pre-coated with 0.1% gelatin. The next day, the medium was changed to EGM2 supplemented with antibiotic/antimycotic and thereafter changed every other day. When reaching confluency and upon detection of EC cell colonies (patches with cobblestone appearance), ECs were purified using anti-CD31 coated magnetic beads (MACS Technology, Milteny Biotec). The resulting MACS-purified ECs were further cultured in EGM2 medium and used at passage 2-5 for bulk RNA-sequencing. RNA was extracted from cultured human TECs and NECs using TRIzol (Thermo Fisher Scientific). Starting from 1μg total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries. Libraries were constructed using the KAPA stranded mRNA-seq kit (Sopachem). ENA-FIRST-PUBLIC 2020-03-07T04:08:04Z ENA-LAST-UPDATE 2019-06-05T10:47:14Z External Id SAMEA5693025 INSDC center name Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB) INSDC first public 2020-03-07T04:08:04Z INSDC last update 2019-06-05T10:47:14Z INSDC status public Submitter Id E-MTAB-8031:TEC 9 broker name ArrayExpress cell type endothelial cell common name human disease lung cancer individual 9 organism part lung sample name E-MTAB-8031:TEC 9 sampling site neoplasm scientific_name Homo sapiens sex male