ERX3417301 E-MTAB-8090:Sample 1_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535922 SAMEA5732619 Sample 1_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury none ERX3417302 E-MTAB-8090:Sample 2_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535923 SAMEA5732620 Sample 2_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury none ERX3417303 E-MTAB-8090:Sample 3_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535924 SAMEA5732621 Sample 3_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury none ERX3417304 E-MTAB-8090:Sample 4_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535925 SAMEA5732622 Sample 4_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury thorax muscle puncture ERX3417305 E-MTAB-8090:Sample 5_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535926 SAMEA5732623 Sample 5_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury thorax muscle puncture ERX3417306 E-MTAB-8090:Sample 6_p ERP115940 PRJEB33170 RNA-seq of Drosophila melanogaster haemocytes with and without injury. Samples collected 1h post injury. ERS3535927 SAMEA5732624 Sample 6_p RNA-Seq TRANSCRIPTOMIC RT-PCR Haemocytes from 60 adult w1118 of 3 to 5 days old females for each biological replicate. 3 to 5 days old females Drosophila melanogaster. Unchallenged and clean injury of the thorax with a thin tungsten needle. RNA was was isolated by TRIzol extraction, and purified with Direct-zol RNA kit (Zymo research).The quality of RNA was assessed using Agilent Bioanalyzer 2100 with a RNA 6000 nano kit. Sequencing libraries were prepared using NEBNext Ultr II RNA Library Prep Kit for Illumina (NEB, E7770S) following the manufacturer's instructions. Briefly, 1μg of total RNA was used as input for poly (A) mRNA enrichment using NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S), followed by fragmentation and reverse transcription to generate cDNA. Hairpin adapter was ligated to fragmented double strand cDNA and USER enzyme was used to cleave the hairpin structure. Ampure beads were used to purify adapter-ligated fragments and the purified product was amplified using NEBNext Multiplex Oligos for Illumina (NEB, #E7335S) to genarte sequencing library. The library was quantitated using Qubit DNA High Sensitivity assay and library quality was checked with Bioanalyzer 2100 using Agilent 7500 DNA Kit. Illumina HiSeq 2500 Experimental Factor: injury thorax muscle puncture