ERS14551927 SAMEA112441629 10090 Mus musculus Protocols: The 10-week old female heterozygous mice (B6.129S4-Pdgfratm11(EGFP)Sor/J) used in this study carried one pdgfrα-GFP allele (which does not encode for active PDGFR-α) and one functional pdgfrα allele. AT3-gcsf tumor-bearing CD140a-EGFP mice were euthanized, and lung was harvested and digested. Lung single cell suspension was prepared and stained with primary-fluorophore conjugated antibodies. Lung CD140a+ mesenchymal cells (CD45-CD31-CD326-GFP+) were isolated by fluorescence-activated cell sorting. Cells capture, lysis and RNA extraction were performed using the 10x V3 Chromium technology. Around 12,000 cells were loaded in each lane of the 10x Chromium chip. Barcoding and library preparation were performed with the 10x Chromium system using version 3 chemistry, following the manufacturer's protocol. cDNA was synthesized and amplified as per manufacturer's protocol and amplified to construct an Illumina sequencing library. cDNA and libraries were checked for quality on an Agilent 4200 TapeStation, quantified by KAPA qPCR. ENA-FIRST-PUBLIC 2023-02-09 ENA-LAST-UPDATE 2023-02-09 External Id SAMEA112441629 INSDC center alias THE JACKSON LABORATORY INSDC center name THE JACKSON LABORATORY INSDC first public 2023-02-09T00:15:51Z INSDC last update 2023-02-09T00:15:51Z INSDC status public Submitter Id E-MTAB-12371:Sample 1 age 10 broker name ArrayExpress cell_type mesenchymal cell common name house mouse developmental stage adult disease breast cancer genotype Pdgfratm11(EGFP)Sor immunophenotype CD45-negative; CD31-negative; CD326-negative; CD140a-positive organism part lung sample name E-MTAB-12371:Sample 1 scientific_name Mus musculus sex female strain B6.129S4-Pdgfratm11(EGFP)Sor/J xenograft AT3-gcsf neoplasm