ERS3579578 SAMEA5776041 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776041 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388991 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type fibroblast organism part skin of breast sample name E-MTAB-8142:4820STDY7388991 sampling site dermis sex female ERS3579579 SAMEA5776042 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776042 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388992 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type dermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7388992 sampling site dermis sex female ERS3579580 SAMEA5776043 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776043 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388993 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type endothelium organism part skin of breast sample name E-MTAB-8142:4820STDY7388993 sampling site dermis sex female ERS3579581 SAMEA5776044 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776044 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388994 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type dermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7388994 sampling site dermis sex female ERS3579582 SAMEA5776045 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776045 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388995 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type epidermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7388995 sampling site epidermis sex female ERS3579583 SAMEA5776046 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776046 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388996 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type keratinocyte CD49f- organism part skin of breast sample name E-MTAB-8142:4820STDY7388996 sampling site epidermis sex female ERS3579584 SAMEA5776047 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776047 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:48Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388997 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type keratinocyte CD49f+ organism part skin of breast sample name E-MTAB-8142:4820STDY7388997 sampling site epidermis sex female ERS3579585 SAMEA5776048 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776048 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388998 broker name ArrayExpress common name human developmental stage adult disease normal individual s1 inferred cell type epidermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7388998 sampling site epidermis sex female ERS3579586 SAMEA5776049 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776049 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7388999 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type fibroblast organism part skin of breast sample name E-MTAB-8142:4820STDY7388999 sampling site dermis sex female ERS3579587 SAMEA5776050 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776050 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389000 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type dermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7389000 sampling site dermis sex female ERS3579588 SAMEA5776051 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776051 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389001 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type endothelium organism part skin of breast sample name E-MTAB-8142:4820STDY7389001 sampling site dermis sex female ERS3579589 SAMEA5776052 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776052 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389002 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type dermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7389002 sampling site dermis sex female ERS3579590 SAMEA5776053 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776053 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389003 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type epidermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7389003 sampling site epidermis sex female ERS3579591 SAMEA5776054 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776054 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389004 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type keratinocyte CD49f- organism part skin of breast sample name E-MTAB-8142:4820STDY7389004 sampling site epidermis sex female ERS3579592 SAMEA5776055 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776055 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389005 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type keratinocyte CD49f+ organism part skin of breast sample name E-MTAB-8142:4820STDY7389005 sampling site epidermis sex female ERS3579593 SAMEA5776056 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776056 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389006 broker name ArrayExpress common name human developmental stage adult disease normal individual s2 inferred cell type epidermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7389006 sampling site epidermis sex female ERS3579594 SAMEA5776057 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776057 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389007 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type fibroblast organism part skin of breast sample name E-MTAB-8142:4820STDY7389007 sampling site dermis sex female ERS3579595 SAMEA5776058 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776058 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389008 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type dermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7389008 sampling site dermis sex female ERS3579596 SAMEA5776059 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776059 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389009 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type endothelium organism part skin of breast sample name E-MTAB-8142:4820STDY7389009 sampling site dermis sex female ERS3579597 SAMEA5776060 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776060 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389010 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type dermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7389010 sampling site dermis sex female ERS3579598 SAMEA5776061 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776061 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389011 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type epidermal myeloid organism part skin of breast sample name E-MTAB-8142:4820STDY7389011 sampling site epidermis sex female ERS3579599 SAMEA5776062 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776062 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389012 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type keratinocyte CD49f- organism part skin of breast sample name E-MTAB-8142:4820STDY7389012 sampling site epidermis sex female ERS3579600 SAMEA5776063 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776063 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389013 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type keratinocyte CD49f+ organism part skin of breast sample name E-MTAB-8142:4820STDY7389013 sampling site epidermis sex female ERS3579601 SAMEA5776064 9606 Homo sapiens Protocols: Surplus skin from breast reconstruction surgery was collected in accordance with the Newcastle and North Tyneside 1 Research Ethics Committee at the Royal Victoria Infirmary, Newcastle upon Tyne NHS Foundation Trust (Newcastle Dermatology Biobank - REC reference: 08/H0906/95+5). Skin was cut to thin strips in PBS and the top 200 μm layer was taken using a dermatome with a Pilling Wecprep blade and a .008 gauge Goulian guard. A grid of slits was cut into the skin sheets to aid enzymatic access, then the skin was treated with 2U/ml dispase II (Roche, 04942078001) in RPMI at 37oC for 1 hour. The epidermis was peeled from the dermis, and both fragments were separately digested in a petri-dish at 37oC 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (roughly 12 hours). All work was done within class II biological safety cabinets using autoclave-sterilised equipment. The media was then collected with a serological pipette and filtered through a sterile 100 μm cell strainer (BD Falcon, 352360). The petri dish and strainer were washed through with RF-10 to collect any remaining cells. Cells were pelleted by centrifuging at 500 x g for 5 minutes. Supernatant was discarded and the pellet resuspended in 1 ml RF-10 by gently pipetting up and down. Cells were then counted by taking off 10 μl, mixing 1:1 with 0.4% trypan blue (Sigma, T8154) to stain dead cells and counting on a haemocytometer. 7000 live, single cells were loaded on to each of the Chromium Controller (10x Genomics, Pleasanton, CA, USA), then cDNA synthesis and amplification were performed as per the 10x Genomics protocol. Sequencing libraries were generated using the Single Cell 3' Reagent Kit as per the 10x Genomics protocol. ENA first public 2020-08-01 ENA last update 2019-07-15 External Id SAMEA5776064 INSDC Status suppressed INSDC center alias Sanger Institute INSDC center name Sanger Institute INSDC first public 2020-08-01T04:03:58Z INSDC last update 2019-07-15T23:03:49Z INSDC status public Submitter Id E-MTAB-8142:4820STDY7389014 broker name ArrayExpress common name human developmental stage adult disease normal individual s3 inferred cell type epidermal lymphoid organism part skin of breast sample name E-MTAB-8142:4820STDY7389014 sampling site epidermis sex female