ERS14634570 SAMEA112642601 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642601 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 1 age 78 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia LGD individual A1 maximum lesion diameter 20 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification 0-IP pit pattern classification IV sample name E-MTAB-12612:Sample 1 sampling site pre-lesion scientific_name Homo sapiens sequencing date 13/12/20 sex female ERS14634571 SAMEA112642602 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642602 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 10 age 63 broker name ArrayExpress colon segment A common name human disease adenoma individual A5 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 10 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634572 SAMEA112642603 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642603 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 100 age 54 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp individual S10 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 100 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634573 SAMEA112642604 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642604 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 101 age 61 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp individual S11 maximum lesion diameter 30 microscopic appearance SSL number of lesions at study colonoscopy 0 organism part colon paris classification LST-NG 0-IIA pit pattern classification II sample name E-MTAB-12612:Sample 101 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634574 SAMEA112642605 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642605 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 102 age 61 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp individual S11 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 102 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634575 SAMEA112642606 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642606 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 103 age 58 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S12 maximum lesion diameter 35 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification LST-NG 0-IIB pit pattern classification II sample name E-MTAB-12612:Sample 103 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634576 SAMEA112642607 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642607 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 104 age 58 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S12 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 104 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634577 SAMEA112642608 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642608 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 105 age 74 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S13 maximum lesion diameter 15 microscopic appearance SSL number of lesions at study colonoscopy 4 organism part colon paris classification LST-NG 0-IIA pit pattern classification II sample name E-MTAB-12612:Sample 105 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634578 SAMEA112642609 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642609 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 106 age 74 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S13 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 106 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634579 SAMEA112642610 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642610 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 107 age 71 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S14 maximum lesion diameter 15 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification 0-IS pit pattern classification II sample name E-MTAB-12612:Sample 107 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634580 SAMEA112642611 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642611 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 108 age 71 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S14 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 108 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634581 SAMEA112642612 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642612 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 109 age 52 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia Dysplasia individual S15 maximum lesion diameter 30 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification IIA pit pattern classification IV sample name E-MTAB-12612:Sample 109 sampling site pre-lesion scientific_name Homo sapiens sequencing date 25/02/21 sex male ERS14634582 SAMEA112642613 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642613 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 11 age 76 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia LGD individual A6 maximum lesion diameter 30 microscopic appearance TA number of lesions at study colonoscopy 3 organism part colon paris classification II-A (LST-G) pit pattern classification IV sample name E-MTAB-12612:Sample 11 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634583 SAMEA112642614 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642614 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 110 age 52 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp individual S15 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 110 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 25/02/21 sex male ERS14634584 SAMEA112642615 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642615 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 111 age 69 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S16 maximum lesion diameter 30 microscopic appearance SSL number of lesions at study colonoscopy 4 organism part colon paris classification LST-NG pit pattern classification II sample name E-MTAB-12612:Sample 111 sampling site pre-lesion scientific_name Homo sapiens sequencing date 25/02/21 sex female ERS14634585 SAMEA112642616 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642616 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 112 age 69 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S16 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 112 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 25/02/21 sex female ERS14634586 SAMEA112642617 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642617 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 113 age 57 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S17 maximum lesion diameter 25 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification IIA (LST-G) pit pattern classification II sample name E-MTAB-12612:Sample 113 sampling site pre-lesion scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634587 SAMEA112642618 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642618 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 114 age 57 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S17 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 114 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634588 SAMEA112642619 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642619 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 115 age 69 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S18 maximum lesion diameter 30 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification LST-NG pit pattern classification II sample name E-MTAB-12612:Sample 115 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634589 SAMEA112642620 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642620 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 116 age 69 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S18 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 116 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634590 SAMEA112642621 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642621 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 117 age 64 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S19 maximum lesion diameter 45 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification LST-NG 0-IIA pit pattern classification II sample name E-MTAB-12612:Sample 117 sampling site pre-lesion scientific_name Homo sapiens sequencing date 08/10/21 sex male ERS14634591 SAMEA112642622 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642622 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 118 age 64 broker name ArrayExpress colon segment C common name human disease Colon Sessile Serrated Adenoma/Polyp individual S19 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 118 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 08/10/21 sex male ERS14634592 SAMEA112642623 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642623 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 12 age 76 broker name ArrayExpress colon segment T common name human disease adenoma individual A6 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 12 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634593 SAMEA112642624 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642624 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 13 age 70 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A7 maximum lesion diameter 50 microscopic appearance TA number of lesions at study colonoscopy 18 organism part colon paris classification LST-NG pit pattern classification II sample name E-MTAB-12612:Sample 13 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634594 SAMEA112642625 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642625 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 14 age 70 broker name ArrayExpress colon segment C common name human disease adenoma individual A7 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 14 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634595 SAMEA112642626 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642626 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 15 age 66 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A8 maximum lesion diameter 25 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification IIA (LST-G) pit pattern classification IIIL sample name E-MTAB-12612:Sample 15 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634596 SAMEA112642627 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642627 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 16 age 66 broker name ArrayExpress colon segment A common name human disease adenoma individual A8 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 16 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634597 SAMEA112642628 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642628 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 17 age 77 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A9 maximum lesion diameter 35 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification IIA/IIC (LST-G) pit pattern classification IV/V sample name E-MTAB-12612:Sample 17 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634598 SAMEA112642629 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642629 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 18 age 77 broker name ArrayExpress colon segment A common name human disease adenoma individual A9 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 18 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634599 SAMEA112642630 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642630 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 19 age 89 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A10 maximum lesion diameter 40 microscopic appearance TA number of lesions at study colonoscopy 4 organism part colon paris classification LST-G (0-IIA) pit pattern classification IV sample name E-MTAB-12612:Sample 19 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634600 SAMEA112642631 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642631 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 2 age 78 broker name ArrayExpress colon segment S common name human disease adenoma individual A1 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 2 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 13/12/20 sex female ERS14634601 SAMEA112642632 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642632 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 20 age 89 broker name ArrayExpress colon segment A common name human disease adenoma individual A10 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 20 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634602 SAMEA112642633 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642633 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 21 age 62 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia HGD individual A11 maximum lesion diameter 70 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification IIA (LST-G) pit pattern classification IV sample name E-MTAB-12612:Sample 21 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634603 SAMEA112642634 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642634 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 22 age 62 broker name ArrayExpress colon segment C common name human disease adenoma individual A11 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 22 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634604 SAMEA112642635 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642635 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 23 age 61 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A12 maximum lesion diameter 25 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification IIA pit pattern classification IIIL sample name E-MTAB-12612:Sample 23 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634605 SAMEA112642636 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642636 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 24 age 61 broker name ArrayExpress colon segment C common name human disease adenoma individual A12 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 24 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634606 SAMEA112642637 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642637 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 25 age 72 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A13 maximum lesion diameter 20 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification 0-IS (RECIDIVA) pit pattern classification IV sample name E-MTAB-12612:Sample 25 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634607 SAMEA112642638 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642638 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 26 age 72 broker name ArrayExpress colon segment A common name human disease adenoma individual A13 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 26 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634608 SAMEA112642639 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642639 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 27 age 62 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A14 maximum lesion diameter 40 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G MIXED 0-IS/O-IIA pit pattern classification IIIL sample name E-MTAB-12612:Sample 27 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634609 SAMEA112642640 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642640 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 28 age 62 broker name ArrayExpress colon segment A common name human disease adenoma individual A14 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 28 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634610 SAMEA112642641 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642641 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 29 age 77 broker name ArrayExpress colon segment R common name human disease adenoma dysplasia LGD individual A15 maximum lesion diameter 20 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification 0-IIA+IS pit pattern classification IV sample name E-MTAB-12612:Sample 29 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634611 SAMEA112642642 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642642 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 3 age 68 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A2 maximum lesion diameter 35 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification ISP pit pattern classification IV sample name E-MTAB-12612:Sample 3 sampling site pre-lesion scientific_name Homo sapiens sequencing date 13/12/20 sex male ERS14634612 SAMEA112642643 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642643 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 30 age 77 broker name ArrayExpress colon segment R common name human disease adenoma individual A15 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 30 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634613 SAMEA112642644 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642644 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 31 age 78 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A16 maximum lesion diameter 35 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G IIA+IIC pit pattern classification IIIL sample name E-MTAB-12612:Sample 31 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634614 SAMEA112642645 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642645 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 32 age 78 broker name ArrayExpress colon segment C common name human disease adenoma individual A16 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 32 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex female ERS14634615 SAMEA112642646 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642646 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 33 age 64 broker name ArrayExpress colon segment D common name human disease adenoma dysplasia LGD individual A17 maximum lesion diameter 45 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification IIA (LST-NG) pit pattern classification IV sample name E-MTAB-12612:Sample 33 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex female ERS14634616 SAMEA112642647 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642647 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 34 age 64 broker name ArrayExpress colon segment D common name human disease adenoma individual A17 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 34 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex female ERS14634617 SAMEA112642648 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642648 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 35 age 57 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A18 maximum lesion diameter 30 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G pit pattern classification IIIL sample name E-MTAB-12612:Sample 35 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex female ERS14634618 SAMEA112642649 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642649 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 36 age 57 broker name ArrayExpress colon segment C common name human disease adenoma individual A18 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 36 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex female ERS14634619 SAMEA112642650 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642650 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 37 age 61 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia LGD individual A19 maximum lesion diameter 30 microscopic appearance TA number of lesions at study colonoscopy 2 organism part colon paris classification LST-NG (IIA/IIC) pit pattern classification IIIL/IIIS sample name E-MTAB-12612:Sample 37 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634620 SAMEA112642651 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642651 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 38 age 61 broker name ArrayExpress colon segment T common name human disease adenoma individual A19 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 38 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634621 SAMEA112642652 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642652 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 39 age 79 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A20 maximum lesion diameter 35 microscopic appearance TVA number of lesions at study colonoscopy 2 organism part colon paris classification 0-ISP pit pattern classification IV sample name E-MTAB-12612:Sample 39 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634622 SAMEA112642653 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642653 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 4 age 68 broker name ArrayExpress colon segment A common name human disease adenoma individual A2 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 4 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 13/12/20 sex male ERS14634623 SAMEA112642654 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642654 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 40 age 79 broker name ArrayExpress colon segment C common name human disease adenoma individual A20 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 40 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634624 SAMEA112642655 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642655 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 41 age 46 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia LGD individual A21 maximum lesion diameter 30 microscopic appearance TVA number of lesions at study colonoscopy 3 organism part colon paris classification 0-IP pit pattern classification IV sample name E-MTAB-12612:Sample 41 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634625 SAMEA112642656 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642656 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 42 age 46 broker name ArrayExpress colon segment S common name human disease adenoma individual A21 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 42 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634626 SAMEA112642657 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642657 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 43 age 67 broker name ArrayExpress colon segment D common name human disease adenoma dysplasia LGD individual A22 maximum lesion diameter 35 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification 0-IS+IIA (LST-G) pit pattern classification IV sample name E-MTAB-12612:Sample 43 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634627 SAMEA112642658 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642658 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 44 age 67 broker name ArrayExpress colon segment D common name human disease adenoma individual A22 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 44 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634628 SAMEA112642659 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642659 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 45 age 83 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A23 maximum lesion diameter 20 microscopic appearance TA number of lesions at study colonoscopy 3 organism part colon paris classification LST 0-IIB pit pattern classification IIIL sample name E-MTAB-12612:Sample 45 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634629 SAMEA112642660 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642660 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 46 age 83 broker name ArrayExpress colon segment C common name human disease adenoma individual A23 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 46 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634630 SAMEA112642661 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642661 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 47 age 69 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia LGD individual A24 maximum lesion diameter 30 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification LST-NG 0-IIA+IS pit pattern classification IIIL sample name E-MTAB-12612:Sample 47 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634631 SAMEA112642662 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642662 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 48 age 69 broker name ArrayExpress colon segment S common name human disease adenoma individual A24 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 48 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634632 SAMEA112642663 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642663 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 49 age 75 broker name ArrayExpress colon segment R common name human disease adenoma dysplasia HGD individual A25 maximum lesion diameter 40 microscopic appearance TVA number of lesions at study colonoscopy 3 organism part colon paris classification LST-G 0-IIA+IS pit pattern classification IV sample name E-MTAB-12612:Sample 49 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634633 SAMEA112642664 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642664 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 5 age 58 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia HGD individual A3 maximum lesion diameter 50 microscopic appearance TVA number of lesions at study colonoscopy 4 organism part colon paris classification LST-G pit pattern classification IV sample name E-MTAB-12612:Sample 5 sampling site pre-lesion scientific_name Homo sapiens sequencing date 25/02/21 sex female ERS14634634 SAMEA112642665 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642665 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 50 age 75 broker name ArrayExpress colon segment R common name human disease adenoma individual A25 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 50 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634635 SAMEA112642666 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642666 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 51 age 64 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia LGD individual A26 maximum lesion diameter 25 microscopic appearance TA number of lesions at study colonoscopy 3 organism part colon paris classification 0-IS pit pattern classification IIIL sample name E-MTAB-12612:Sample 51 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634636 SAMEA112642667 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642667 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 52 age 64 broker name ArrayExpress colon segment T common name human disease adenoma individual A26 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 52 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634637 SAMEA112642668 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642668 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 53 age 50 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A27 maximum lesion diameter 15 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification 0-ISP pit pattern classification IV sample name E-MTAB-12612:Sample 53 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634638 SAMEA112642669 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642669 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 54 age 50 broker name ArrayExpress colon segment A common name human disease adenoma individual A27 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 54 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634639 SAMEA112642670 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642670 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 55 age 79 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia HGD individual A28 maximum lesion diameter 70 microscopic appearance TVA number of lesions at study colonoscopy 3 organism part colon paris classification 0-IS pit pattern classification IV sample name E-MTAB-12612:Sample 55 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634640 SAMEA112642671 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642671 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 56 age 79 broker name ArrayExpress colon segment T common name human disease adenoma individual A28 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 56 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634641 SAMEA112642672 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642672 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 57 age 63 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A29 maximum lesion diameter 30 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G (0-IIA) pit pattern classification IIIL sample name E-MTAB-12612:Sample 57 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634642 SAMEA112642673 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642673 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 58 age 63 broker name ArrayExpress colon segment C common name human disease adenoma individual A29 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 58 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634643 SAMEA112642674 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642674 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 59 age 63 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A30 maximum lesion diameter 40 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G NODULAR 0-IIA pit pattern classification IV sample name E-MTAB-12612:Sample 59 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634644 SAMEA112642675 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642675 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 6 age 58 broker name ArrayExpress colon segment T common name human disease adenoma individual A3 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 6 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 25/02/21 sex female ERS14634645 SAMEA112642676 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642676 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 60 age 63 broker name ArrayExpress colon segment C common name human disease adenoma individual A30 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 60 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex female ERS14634646 SAMEA112642677 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642677 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 61 age 68 broker name ArrayExpress colon segment R common name human disease adenoma dysplasia HGD individual A31 maximum lesion diameter 60 microscopic appearance TA number of lesions at study colonoscopy 3 organism part colon paris classification LST-G O-IIA pit pattern classification IIIL+IV sample name E-MTAB-12612:Sample 61 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634647 SAMEA112642678 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642678 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 62 age 68 broker name ArrayExpress colon segment R common name human disease adenoma individual A31 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 62 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 20/08/21 sex male ERS14634648 SAMEA112642679 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642679 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 63 age 65 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A32 maximum lesion diameter 40 microscopic appearance TA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G 0-IIA+IIC pit pattern classification III-L+IV sample name E-MTAB-12612:Sample 63 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634649 SAMEA112642680 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642680 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 64 age 65 broker name ArrayExpress colon segment A common name human disease adenoma individual A32 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 64 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634650 SAMEA112642681 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642681 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 65 age 72 broker name ArrayExpress colon segment R common name human disease adenoma dysplasia LGD individual A33 maximum lesion diameter 45 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification 0-IS pit pattern classification IV sample name E-MTAB-12612:Sample 65 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634651 SAMEA112642682 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642682 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 66 age 72 broker name ArrayExpress colon segment R common name human disease adenoma individual A33 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 66 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex male ERS14634652 SAMEA112642683 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642683 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 67 age 79 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia HGD individual A34 maximum lesion diameter 70 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G pit pattern classification III/IV sample name E-MTAB-12612:Sample 67 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634653 SAMEA112642684 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642684 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 68 age 79 broker name ArrayExpress colon segment S common name human disease adenoma individual A34 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 68 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634654 SAMEA112642685 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642685 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 69 age 62 broker name ArrayExpress colon segment T common name human disease adenoma dysplasia ADC individual A35 maximum lesion diameter 80 microscopic appearance TVA number of lesions at study colonoscopy 3 organism part colon paris classification LST-G NODULAR 0-IIA+IS pit pattern classification VI sample name E-MTAB-12612:Sample 69 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634655 SAMEA112642686 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642686 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 7 age 69 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia HGD individual A4 maximum lesion diameter 50 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification ISP pit pattern classification IV sample name E-MTAB-12612:Sample 7 sampling site pre-lesion scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634656 SAMEA112642687 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642687 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 70 age 62 broker name ArrayExpress colon segment T common name human disease adenoma individual A35 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 70 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634657 SAMEA112642688 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642688 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 71 age 67 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia HGD individual A36 maximum lesion diameter 30 microscopic appearance TVA number of lesions at study colonoscopy 2 organism part colon paris classification LST-NG 0-IIA pit pattern classification II sample name E-MTAB-12612:Sample 71 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634658 SAMEA112642689 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642689 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 72 age 67 broker name ArrayExpress colon segment A common name human disease adenoma individual A36 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 72 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634659 SAMEA112642690 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642690 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 73 age 78 broker name ArrayExpress colon segment R common name human disease adenoma dysplasia LGD individual A37 maximum lesion diameter 50 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G 0-IIA (RECIDIVA) pit pattern classification IV sample name E-MTAB-12612:Sample 73 sampling site pre-lesion scientific_name Homo sapiens sequencing date 08/10/21 sex female ERS14634660 SAMEA112642691 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642691 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 74 age 78 broker name ArrayExpress colon segment R common name human disease adenoma individual A37 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 74 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 08/10/21 sex female ERS14634661 SAMEA112642692 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642692 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 75 age 66 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A38 maximum lesion diameter 35 microscopic appearance TVA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G pit pattern classification IIIL/IV sample name E-MTAB-12612:Sample 75 sampling site pre-lesion scientific_name Homo sapiens sequencing date 08/10/21 sex male ERS14634662 SAMEA112642693 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642693 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 76 age 66 broker name ArrayExpress colon segment A common name human disease adenoma individual A38 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 76 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 08/10/21 sex male ERS14634663 SAMEA112642694 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642694 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 77 age 70 broker name ArrayExpress colon segment C common name human disease adenoma dysplasia LGD individual A39 maximum lesion diameter 12 microscopic appearance TA number of lesions at study colonoscopy 8 organism part colon paris classification IIa pit pattern classification IIIs sample name E-MTAB-12612:Sample 77 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex female ERS14634664 SAMEA112642695 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642695 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 78 age 70 broker name ArrayExpress colon segment C common name human disease adenoma individual A39 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 78 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex female ERS14634665 SAMEA112642696 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642696 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 79 age 69 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia LGD individual A40 maximum lesion diameter 22 microscopic appearance TA organism part colon paris classification Is pit pattern classification IV sample name E-MTAB-12612:Sample 79 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634666 SAMEA112642697 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642697 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 8 age 69 broker name ArrayExpress colon segment A common name human disease adenoma individual A4 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 8 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634667 SAMEA112642698 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642698 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 80 age 69 broker name ArrayExpress colon segment S common name human disease adenoma individual A40 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 80 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634668 SAMEA112642699 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642699 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 81 age 71 broker name ArrayExpress colon segment S common name human disease adenoma dysplasia LGD individual A41 maximum lesion diameter 24 microscopic appearance TVA number of lesions at study colonoscopy 4 organism part colon paris classification Ip pit pattern classification IV sample name E-MTAB-12612:Sample 81 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634669 SAMEA112642700 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642700 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 82 age 71 broker name ArrayExpress colon segment S common name human disease adenoma individual A41 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 82 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/07/21 sex male ERS14634670 SAMEA112642701 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642701 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 83 age 72 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S1 maximum lesion diameter 45 microscopic appearance SSL number of lesions at study colonoscopy 2 organism part colon paris classification LST-G 0-IIA+IS pit pattern classification IIIL+IV sample name E-MTAB-12612:Sample 83 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634671 SAMEA112642702 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642702 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 84 age 72 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S1 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 84 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634672 SAMEA112642703 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642703 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 85 age 75 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S2 maximum lesion diameter 40 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification LST-NG 0-IIA pit pattern classification II+IIIL sample name E-MTAB-12612:Sample 85 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634673 SAMEA112642704 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642704 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 86 age 75 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S2 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 86 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex male ERS14634674 SAMEA112642705 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642705 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 87 age 60 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S3 maximum lesion diameter 15 microscopic appearance SSL number of lesions at study colonoscopy 3 organism part colon paris classification LST-NG pit pattern classification II sample name E-MTAB-12612:Sample 87 sampling site pre-lesion scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634675 SAMEA112642706 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642706 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 88 age 60 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S3 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 88 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 29/04/21 sex female ERS14634676 SAMEA112642707 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642707 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 89 age 80 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S4 maximum lesion diameter 25 microscopic appearance SSL number of lesions at study colonoscopy 2 organism part colon paris classification LST-NG 0-IIA pit pattern classification II sample name E-MTAB-12612:Sample 89 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634677 SAMEA112642708 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642708 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 9 age 63 broker name ArrayExpress colon segment A common name human disease adenoma dysplasia LGD individual A5 maximum lesion diameter 60 microscopic appearance VA number of lesions at study colonoscopy 1 organism part colon paris classification LST-G O-IIA+IS pit pattern classification IV sample name E-MTAB-12612:Sample 9 sampling site pre-lesion scientific_name Homo sapiens sequencing date 15/04/21 sex female ERS14634678 SAMEA112642709 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642709 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 90 age 80 broker name ArrayExpress colon segment A common name human disease Colon Sessile Serrated Adenoma/Polyp individual S4 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 90 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/06/21 sex male ERS14634679 SAMEA112642710 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642710 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 91 age 55 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S6 maximum lesion diameter 25 microscopic appearance SSL number of lesions at study colonoscopy 2 organism part colon paris classification LST-G (IIA) pit pattern classification II sample name E-MTAB-12612:Sample 91 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634680 SAMEA112642711 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642711 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 92 age 55 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S6 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 92 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634681 SAMEA112642712 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642712 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 93 age 50 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S7 maximum lesion diameter 20 microscopic appearance SSL number of lesions at study colonoscopy 6 organism part colon paris classification 0-IS pit pattern classification II sample name E-MTAB-12612:Sample 93 sampling site pre-lesion scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634682 SAMEA112642713 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642713 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 94 age 50 broker name ArrayExpress colon segment T common name human disease Colon Sessile Serrated Adenoma/Polyp individual S7 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 94 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 16/07/21 sex male ERS14634683 SAMEA112642714 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642714 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 95 age 62 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S8 maximum lesion diameter 12 microscopic appearance SSL number of lesions at study colonoscopy 2 organism part colon paris classification II-A pit pattern classification II sample name E-MTAB-12612:Sample 95 sampling site pre-lesion scientific_name Homo sapiens sequencing date 08/10/21 sex female ERS14634684 SAMEA112642715 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642715 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 96 age 62 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp individual S8 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 96 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 08/10/21 sex female ERS14634685 SAMEA112642716 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642716 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 97 age 71 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp individual S9 maximum lesion diameter 15 microscopic appearance SSL number of lesions at study colonoscopy 1 organism part colon paris classification 0-IIB pit pattern classification II sample name E-MTAB-12612:Sample 97 sampling site pre-lesion scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634686 SAMEA112642717 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642717 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 98 age 71 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp individual S9 microscopic appearance normal organism part colon paris classification normal pit pattern classification normal sample name E-MTAB-12612:Sample 98 sampling site normal tissue adjacent to pre-lesion (>2cm) scientific_name Homo sapiens sequencing date 01/10/21 sex female ERS14634687 SAMEA112642718 9606 Homo sapiens Protocols: This validation study was performed on 41 cADNs, 18 SSLs, and their adjacent normal mucosa samples (total number of samples analyzed = 118). Colorectal tissues were prospectively collected during colonoscopies. Endoscopic biopsies from the tumor and the corresponding normal mucosa (located >2 cm from the tumor) were immersed in AllProtect Tissue Reagent (Qiagen, Hilden, Germany), held at 4°C overnight, and stored at -80°C. onors provided written consent to tissue collection, testing and data publications. Samples were numerically coded to protect donors' rights to confidentiality and privacy. The study was approved by the Zurich canton ethics committee. DNA extraction was performed with Qiagen AllPrep DNA/RNA mini kit (Hilden, Germany). DNA concentrations were quantified using Qubit fluorometer (Invitrogen, Carlsbad, USA) and quality was assessed with NanoDrop (ThermoFisher Scientific, Wilmington, USA). Genomic DNA (100ng) was bisulfite converted with EZ-DNA Methylation-Gold kit (Zymo, Irvine, USA) prior to library preparation which was performed with Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences, Ann Arbor, USA). Eight indexed libraries were pooled in equimolar amounts for hybridization with baits. Hybridization of customized RNA baits with the pool of 8 libraries was performed twice at 63°C for 16 to 24 hours. AMPure Xp beads (Beckman Coulter Inc, Brea, USA) were used to isolate biotinylated DNA, and amplification of bead-bound enriched libraries was performed using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems, Wilmington, MA, USA) and adaptor specific primers. The concentration of the enriched libraries was determined with a Qubit fluorometer, and library size with a Tapestation (Agilent, Santa Clara, CA, USA) for subsequent library titration and next generation sequencing. ENA-FIRST-PUBLIC 2023-03-30 ENA-LAST-UPDATE 2023-03-30 External Id SAMEA112642718 INSDC center alias UZH INSDC center name University of Zurich INSDC first public 2023-03-30T12:17:29Z INSDC last update 2023-03-30T12:17:29Z INSDC status public Submitter Id E-MTAB-12612:Sample 99 age 54 broker name ArrayExpress colon segment D common name human disease Colon Sessile Serrated Adenoma/Polyp dysplasia LGD individual S10 maximum lesion diameter 25 microscopic appearance SSL number of lesions at study colonoscopy 4 organism part colon paris classification LST-NG (0-IIA) pit pattern classification II sample name E-MTAB-12612:Sample 99 sampling site pre-lesion scientific_name Homo sapiens sequencing date 20/08/21 sex male