ERX10332738 E-MTAB-12616:BL6_d21_B_T_Mono_Neutro_3_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634688 SAMEA112642719 BL6_d21_B_T_Mono_Neutro_3_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease multiple myeloma Experimental Factor: strain C57Bl/6JOlaHsd ERX10332739 E-MTAB-12616:BL6_d22_B_T_mono_neutro_2_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634689 SAMEA112642720 BL6_d22_B_T_mono_neutro_2_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease multiple myeloma Experimental Factor: strain C57Bl/6JOlaHsd ERX10332740 E-MTAB-12616:BL6_SS_1_B_T_mono_neutro_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634690 SAMEA112642721 BL6_SS_1_B_T_mono_neutro_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease normal Experimental Factor: strain C57Bl/6JOlaHsd ERX10332741 E-MTAB-12616:BL6_SS_2_B_T_mono_neutro_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634691 SAMEA112642722 BL6_SS_2_B_T_mono_neutro_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease normal Experimental Factor: strain C57Bl/6JOlaHsd ERX10332742 E-MTAB-12616:Kalwrij_d21_nr3_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634692 SAMEA112642723 Kalwrij_d21_nr3_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease multiple myeloma Experimental Factor: strain C57Bl/KaLwRijHsd ERX10332743 E-MTAB-12616:Kalwrij_d21_nr4_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634693 SAMEA112642724 Kalwrij_d21_nr4_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease multiple myeloma Experimental Factor: strain C57Bl/KaLwRijHsd ERX10332744 E-MTAB-12616:Kalwrij_SS_nr1_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634694 SAMEA112642725 Kalwrij_SS_nr1_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease normal Experimental Factor: strain C57Bl/KaLwRijHsd ERX10332745 E-MTAB-12616:Kalwrij_SS_nr2_p ERP144948 PRJEB59902 scRNAseq of the bone marrow immune microenvironment in control and 5TGM1 myeloma-bearing C57Bl/6 and KaLwRij mice ERS14634695 SAMEA112642726 Kalwrij_SS_nr2_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer’s recommendations (10X Genomics) Illumina NovaSeq 6000 Experimental Factor: disease normal Experimental Factor: strain C57Bl/KaLwRijHsd