ERS14634688 SAMEA112642719 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642719 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:BL6_d21_B_T_Mono_Neutro_3 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease multiple myeloma genotype wild type genotype individual B_mm_3 organism part bone marrow sample name E-MTAB-12616:BL6_d21_B_T_Mono_Neutro_3 scientific_name Mus musculus sex female strain C57Bl/6JOlaHsd ERS14634689 SAMEA112642720 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642720 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:BL6_d22_B_T_mono_neutro_2 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease multiple myeloma genotype wild type genotype individual B_mm_2 organism part bone marrow sample name E-MTAB-12616:BL6_d22_B_T_mono_neutro_2 scientific_name Mus musculus sex female strain C57Bl/6JOlaHsd ERS14634690 SAMEA112642721 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642721 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:BL6_SS_1_B_T_mono_neutro age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease normal genotype wild type genotype individual B_ss_1 organism part bone marrow sample name E-MTAB-12616:BL6_SS_1_B_T_mono_neutro scientific_name Mus musculus sex female strain C57Bl/6JOlaHsd ERS14634691 SAMEA112642722 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642722 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:BL6_SS_2_B_T_mono_neutro age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease normal genotype wild type genotype individual B_ss_2 organism part bone marrow sample name E-MTAB-12616:BL6_SS_2_B_T_mono_neutro scientific_name Mus musculus sex female strain C57Bl/6JOlaHsd ERS14634692 SAMEA112642723 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642723 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:Kalwrij_d21_nr3 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease multiple myeloma genotype wild type genotype individual K_mm_3 organism part bone marrow sample name E-MTAB-12616:Kalwrij_d21_nr3 scientific_name Mus musculus sex female strain C57Bl/KaLwRijHsd ERS14634693 SAMEA112642724 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642724 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:Kalwrij_d21_nr4 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease multiple myeloma genotype wild type genotype individual K_mm_4 organism part bone marrow sample name E-MTAB-12616:Kalwrij_d21_nr4 scientific_name Mus musculus sex female strain C57Bl/KaLwRijHsd ERS14634694 SAMEA112642725 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642725 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:Kalwrij_SS_nr1 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease normal genotype wild type genotype individual K_ss_1 organism part bone marrow sample name E-MTAB-12616:Kalwrij_SS_nr1 scientific_name Mus musculus sex female strain C57Bl/KaLwRijHsd ERS14634695 SAMEA112642726 10090 Mus musculus Protocols: 1 femur and 2 tibias from each mouse were used. After isolating and cleaning the bones, one end was cut off and bones were placed into a 0.5 ml microcentrifuge tube with a hole punched in the bottom. This was nested into a 1.5 ml microcentrifuge tube and spun down at 10,000 RPM for 15 seconds to collect the bone marrow. Following resuspension in PBS containing 1% FCS and 1 mM EDTA, erythrocytes were lysed. Remaining cells were washed with PBS containing 2% FCS, filtered through a 100 um cell strainer and counted. Following erythrocyte lysis, the samples were filtered through a 100 um cell strainer and counted. Fc-receptors were blocked with 10% normal mouse serum and anti-CD16/32 (BD, clone 2.4G2) prior to incubation with the following antibodies: CD3e-PerCpCy5.5 (eBioscience, clone 145-2C11), CD8-FITC (BD, clone 53-6.7), CD11b-APCeFluor780 (eBioscience, clone M1/70), CD19-AF700 (eBioscience, clone eBio1D3), CD27-BV510 (Biolegend, clone LG.3A10), CD44-BV510 (Biolegend, clone IM7), NKp46-PE-Cy7 (eBioscience, clone 29A1.4), Gr1-APC (BD, clone RB6-8C5), Ly6c-BV510 (Biolegend, clone HK1.4). Samples were incubated for 25 minutes on ice in the dark. Following staining, cells were washed and resuspended in PBS + 2% FCS containing DAPI, and sorted with a BD FACS Aria III. Sorted T, NK, B, monocyte/macrophages and neutrophil granulocytes were combined per mouse and processed as one sample. Single cells were encapsulated for cDNA synthesis and barcoded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics) Libraries were constructed according to the manufacturer's recommendations (10X Genomics) ENA-FIRST-PUBLIC 2023-02-23 ENA-LAST-UPDATE 2023-02-23 External Id SAMEA112642726 INSDC center alias Department of Hematology Erasmus MC INSDC center name Department of Hematology Erasmus MC INSDC first public 2023-02-23T00:28:36Z INSDC last update 2023-02-23T00:28:36Z INSDC status public Submitter Id E-MTAB-12616:Kalwrij_SS_nr2 age 12 broker name ArrayExpress cell_type bone marrow cell common name house mouse developmental stage adult disease normal genotype wild type genotype individual K_ss_2 organism part bone marrow sample name E-MTAB-12616:Kalwrij_SS_nr2 scientific_name Mus musculus sex female strain C57Bl/KaLwRijHsd