ERS3646885 SAMEA5858368 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:29Z External Id SAMEA5858368 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:29Z INSDC status public Submitter Id E-MTAB-8231:BA1-1 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-1 scientific_name Pan paniscus segment order 1 sex male ERS3646886 SAMEA5858369 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:29Z External Id SAMEA5858369 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:29Z INSDC status public Submitter Id E-MTAB-8231:BA1-10 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-10 scientific_name Pan paniscus segment order 10 sex male ERS3646887 SAMEA5858370 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:29Z External Id SAMEA5858370 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:29Z INSDC status public Submitter Id E-MTAB-8231:BA1-2 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-2 scientific_name Pan paniscus segment order 2 sex male ERS3646888 SAMEA5858371 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858371 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-3 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-3 scientific_name Pan paniscus segment order 3 sex male ERS3646889 SAMEA5858372 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858372 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-4 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-4 scientific_name Pan paniscus segment order 4 sex male ERS3646890 SAMEA5858373 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858373 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-5 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-5 scientific_name Pan paniscus segment order 5 sex male ERS3646891 SAMEA5858374 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858374 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-6 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-6 scientific_name Pan paniscus segment order 6 sex male ERS3646892 SAMEA5858375 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858375 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-7 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-7 scientific_name Pan paniscus segment order 7 sex male ERS3646893 SAMEA5858376 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858376 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-8 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-8 scientific_name Pan paniscus segment order 8 sex male ERS3646894 SAMEA5858377 9597 Pan paniscus Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858377 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:BA1-9 age 15 broker name ArrayExpress common name pygmy chimpanzee developmental stage adult disease normal individual BA1 organism part prefrontal cortex sample name E-MTAB-8231:BA1-9 scientific_name Pan paniscus segment order 9 sex male ERS3646895 SAMEA5858378 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858378 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-1 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-1 scientific_name Pan troglodytes segment order 1 sex female ERS3646896 SAMEA5858379 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858379 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-10 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-10 scientific_name Pan troglodytes segment order 10 sex female ERS3646897 SAMEA5858380 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858380 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-2 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-2 scientific_name Pan troglodytes segment order 2 sex female ERS3646898 SAMEA5858381 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858381 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-3 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-3 scientific_name Pan troglodytes segment order 3 sex female ERS3646899 SAMEA5858382 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858382 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-4 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-4 scientific_name Pan troglodytes segment order 4 sex female ERS3646900 SAMEA5858383 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858383 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-5 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-5 scientific_name Pan troglodytes segment order 5 sex female ERS3646901 SAMEA5858384 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858384 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-6 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-6 scientific_name Pan troglodytes segment order 6 sex female ERS3646902 SAMEA5858385 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858385 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-7 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-7 scientific_name Pan troglodytes segment order 7 sex female ERS3646903 SAMEA5858386 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858386 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-8 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-8 scientific_name Pan troglodytes segment order 8 sex female ERS3646904 SAMEA5858387 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858387 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHD2-9 age 45 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHD2 organism part prefrontal cortex sample name E-MTAB-8231:CHD2-9 scientific_name Pan troglodytes segment order 9 sex female ERS3646905 SAMEA5858388 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858388 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-1 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-1 scientific_name Pan troglodytes segment order 1 sex male ERS3646906 SAMEA5858389 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858389 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-10 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-10 scientific_name Pan troglodytes segment order 10 sex male ERS3646907 SAMEA5858390 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858390 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-2 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-2 scientific_name Pan troglodytes segment order 2 sex male ERS3646908 SAMEA5858391 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858391 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-3 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-3 scientific_name Pan troglodytes segment order 3 sex male ERS3646909 SAMEA5858392 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858392 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-4 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-4 scientific_name Pan troglodytes segment order 4 sex male ERS3646910 SAMEA5858393 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858393 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-5 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-5 scientific_name Pan troglodytes segment order 5 sex male ERS3646911 SAMEA5858394 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858394 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-6 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-6 scientific_name Pan troglodytes segment order 6 sex male ERS3646912 SAMEA5858395 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858395 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-7 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-7 scientific_name Pan troglodytes segment order 7 sex male ERS3646913 SAMEA5858396 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858396 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-8 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-8 scientific_name Pan troglodytes segment order 8 sex male ERS3646914 SAMEA5858397 9598 Pan troglodytes Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858397 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:CHF3-9 age 12 broker name ArrayExpress common name chimpanzee developmental stage adult disease normal individual CHF3 organism part prefrontal cortex sample name E-MTAB-8231:CHF3-9 scientific_name Pan troglodytes segment order 9 sex male ERS3646915 SAMEA5858398 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858398 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-1 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-1 scientific_name Homo sapiens segment order 1 sex female ERS3646916 SAMEA5858399 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858399 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-10 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-10 scientific_name Homo sapiens segment order 10 sex female ERS3646917 SAMEA5858400 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858400 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-2 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-2 scientific_name Homo sapiens segment order 2 sex female ERS3646918 SAMEA5858401 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858401 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-3 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-3 scientific_name Homo sapiens segment order 3 sex female ERS3646919 SAMEA5858402 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858402 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-4 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-4 scientific_name Homo sapiens segment order 4 sex female ERS3646920 SAMEA5858403 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858403 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-5 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-5 scientific_name Homo sapiens segment order 5 sex female ERS3646921 SAMEA5858404 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858404 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-6 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-6 scientific_name Homo sapiens segment order 6 sex female ERS3646922 SAMEA5858405 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858405 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-7 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-7 scientific_name Homo sapiens segment order 7 sex female ERS3646923 SAMEA5858406 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858406 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-8 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-8 scientific_name Homo sapiens segment order 8 sex female ERS3646924 SAMEA5858407 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858407 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HC1-9 age 34 broker name ArrayExpress common name human developmental stage adult disease normal individual HC1 organism part prefrontal cortex sample name E-MTAB-8231:HC1-9 scientific_name Homo sapiens segment order 9 sex female ERS3646925 SAMEA5858408 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858408 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-1 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-1 scientific_name Homo sapiens segment order 1 sex male ERS3646926 SAMEA5858409 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858409 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-10 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-10 scientific_name Homo sapiens segment order 10 sex male ERS3646927 SAMEA5858410 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858410 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-2 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-2 scientific_name Homo sapiens segment order 2 sex male ERS3646928 SAMEA5858411 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858411 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-3 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-3 scientific_name Homo sapiens segment order 3 sex male ERS3646929 SAMEA5858412 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858412 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-4 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-4 scientific_name Homo sapiens segment order 4 sex male ERS3646930 SAMEA5858413 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858413 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-5 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-5 scientific_name Homo sapiens segment order 5 sex male ERS3646931 SAMEA5858414 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858414 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-6 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-6 scientific_name Homo sapiens segment order 6 sex male ERS3646932 SAMEA5858415 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858415 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-7 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-7 scientific_name Homo sapiens segment order 7 sex male ERS3646933 SAMEA5858416 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858416 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-8 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-8 scientific_name Homo sapiens segment order 8 sex male ERS3646934 SAMEA5858417 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858417 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HE1-9 age 17 broker name ArrayExpress common name human developmental stage adult disease normal individual HE1 organism part prefrontal cortex sample name E-MTAB-8231:HE1-9 scientific_name Homo sapiens segment order 9 sex male ERS3646935 SAMEA5858418 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858418 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-1 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-1 scientific_name Homo sapiens segment order 1 sex male ERS3646936 SAMEA5858419 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858419 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-10 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-10 scientific_name Homo sapiens segment order 10 sex male ERS3646937 SAMEA5858420 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858420 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-2 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-2 scientific_name Homo sapiens segment order 2 sex male ERS3646938 SAMEA5858421 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858421 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-3 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-3 scientific_name Homo sapiens segment order 3 sex male ERS3646939 SAMEA5858422 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858422 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-4 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-4 scientific_name Homo sapiens segment order 4 sex male ERS3646940 SAMEA5858423 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858423 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-5 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-5 scientific_name Homo sapiens segment order 5 sex male ERS3646941 SAMEA5858424 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858424 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-6 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-6 scientific_name Homo sapiens segment order 6 sex male ERS3646942 SAMEA5858425 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858425 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-7 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-7 scientific_name Homo sapiens segment order 7 sex male ERS3646943 SAMEA5858426 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:30Z External Id SAMEA5858426 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:30Z INSDC status public Submitter Id E-MTAB-8231:HG1-8 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-8 scientific_name Homo sapiens segment order 8 sex male ERS3646944 SAMEA5858427 9606 Homo sapiens Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858427 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:HG1-9 age 54 broker name ArrayExpress common name human developmental stage adult disease normal individual HG1 organism part prefrontal cortex sample name E-MTAB-8231:HG1-9 scientific_name Homo sapiens segment order 9 sex male ERS3646945 SAMEA5858428 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858428 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-1 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-1 scientific_name Macaca mulatta segment order 1 sex male ERS3646946 SAMEA5858429 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858429 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-10 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-10 scientific_name Macaca mulatta segment order 10 sex male ERS3646947 SAMEA5858430 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858430 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-2 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-2 scientific_name Macaca mulatta segment order 2 sex male ERS3646948 SAMEA5858431 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858431 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-3 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-3 scientific_name Macaca mulatta segment order 3 sex male ERS3646949 SAMEA5858432 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858432 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-4 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-4 scientific_name Macaca mulatta segment order 4 sex male ERS3646950 SAMEA5858433 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858433 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-5 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-5 scientific_name Macaca mulatta segment order 5 sex male ERS3646951 SAMEA5858434 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858434 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-6 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-6 scientific_name Macaca mulatta segment order 6 sex male ERS3646952 SAMEA5858435 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858435 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-7 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-7 scientific_name Macaca mulatta segment order 7 sex male ERS3646953 SAMEA5858436 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858436 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-8 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-8 scientific_name Macaca mulatta segment order 8 sex male ERS3646954 SAMEA5858437 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858437 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MG2-9 age 7 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MG2 organism part prefrontal cortex sample name E-MTAB-8231:MG2-9 scientific_name Macaca mulatta segment order 9 sex male ERS3646955 SAMEA5858438 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858438 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-1 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-1 scientific_name Macaca mulatta segment order 1 sex male ERS3646956 SAMEA5858439 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858439 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-10 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-10 scientific_name Macaca mulatta segment order 10 sex male ERS3646957 SAMEA5858440 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858440 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-2 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-2 scientific_name Macaca mulatta segment order 2 sex male ERS3646958 SAMEA5858441 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858441 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-3 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-3 scientific_name Macaca mulatta segment order 3 sex male ERS3646959 SAMEA5858442 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858442 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-4 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-4 scientific_name Macaca mulatta segment order 4 sex male ERS3646960 SAMEA5858443 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858443 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-5 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-5 scientific_name Macaca mulatta segment order 5 sex male ERS3646961 SAMEA5858444 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858444 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-6 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-6 scientific_name Macaca mulatta segment order 6 sex male ERS3646962 SAMEA5858445 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858445 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-7 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-7 scientific_name Macaca mulatta segment order 7 sex male ERS3646963 SAMEA5858446 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858446 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-8 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-8 scientific_name Macaca mulatta segment order 8 sex male ERS3646964 SAMEA5858447 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858447 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MH1-9 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MH1 organism part prefrontal cortex sample name E-MTAB-8231:MH1-9 scientific_name Macaca mulatta segment order 9 sex male ERS3646965 SAMEA5858448 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858448 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-1 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-1 scientific_name Macaca mulatta segment order 1 sex male ERS3646966 SAMEA5858449 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858449 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-10 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-10 scientific_name Macaca mulatta segment order 10 sex male ERS3646967 SAMEA5858450 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858450 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-2 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-2 scientific_name Macaca mulatta segment order 2 sex male ERS3646968 SAMEA5858451 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858451 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-3 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-3 scientific_name Macaca mulatta segment order 3 sex male ERS3646969 SAMEA5858452 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858452 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-4 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-4 scientific_name Macaca mulatta segment order 4 sex male ERS3646970 SAMEA5858453 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858453 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-5 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-5 scientific_name Macaca mulatta segment order 5 sex male ERS3646971 SAMEA5858454 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858454 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-6 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-6 scientific_name Macaca mulatta segment order 6 sex male ERS3646972 SAMEA5858455 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858455 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-7 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-7 scientific_name Macaca mulatta segment order 7 sex male ERS3646973 SAMEA5858456 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858456 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-8 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-8 scientific_name Macaca mulatta segment order 8 sex male ERS3646974 SAMEA5858457 9544 Macaca mulatta Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit. ENA-FIRST-PUBLIC 2019-10-04T04:04:47Z ENA-LAST-UPDATE 2019-08-08T16:44:31Z External Id SAMEA5858457 INSDC center name Department of Biosystems Science and Engineering, ETH Zurich INSDC first public 2019-10-04T04:04:47Z INSDC last update 2019-08-08T16:44:31Z INSDC status public Submitter Id E-MTAB-8231:MI1-9 age 9 broker name ArrayExpress common name Rhesus monkey developmental stage adult disease normal individual MI1 organism part prefrontal cortex sample name E-MTAB-8231:MI1-9 scientific_name Macaca mulatta segment order 9 sex male