ERS3658737 SAMEA5870249 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870249 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM+T_17 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease acute graft vs. host disease genotype wild type genotype individual 17 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM+T_17 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow + T cells transplant strain C57BL/6 ERS3658738 SAMEA5870250 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870250 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM+T_21 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease acute graft vs. host disease genotype wild type genotype individual 21 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM+T_21 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow + T cells transplant strain C57BL/6 ERS3658739 SAMEA5870251 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870251 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM+T_29 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease acute graft vs. host disease genotype wild type genotype individual 29 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM+T_29 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow + T cells transplant strain C57BL/6 ERS3658740 SAMEA5870252 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870252 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM_68 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 68 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM_68 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow transplant strain C57BL/6 ERS3658741 SAMEA5870253 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870253 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM_69 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 69 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM_69 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow transplant strain C57BL/6 ERS3658742 SAMEA5870254 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870254 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_TCDBM_70 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 70 organism part lymph node sample name E-MTAB-8255:FRC_TCDBM_70 scientific_name Mus musculus sex male stimulus T-cell depleted bone marrow transplant strain C57BL/6 ERS3658743 SAMEA5870255 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870255 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_untransplanted_53 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 53 organism part lymph node sample name E-MTAB-8255:FRC_untransplanted_53 scientific_name Mus musculus sex male strain C57BL/6 ERS3658744 SAMEA5870256 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870256 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_untransplanted_54 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 54 organism part lymph node sample name E-MTAB-8255:FRC_untransplanted_54 scientific_name Mus musculus sex male strain C57BL/6 ERS3658745 SAMEA5870257 10090 Mus musculus Protocols: LN were isolated from recipient mice and digested with Liberase/Dnase at 37°C. Single cell suspension was stained for CD45, gp38 and CD31 to discriminate different lymph node stroma subsets. CD45-CD31-gp38+ FRC were flow sorted and collected in RLT lysis buffer. RNA was extracted using the RNeasy Micro Kit (QIAGEN, USA) following the manufacturers protocol. RNA yield, quality and integrity were evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA samples were amplified using the SMART Seq v4 Ultra Low Input RNA Kit. Paired-end sequencing libraries were prepared from the amplified cDNA according to the Nextera XT DNA library prep protocol. ENA-FIRST-PUBLIC 2020-01-14T04:04:50Z ENA-LAST-UPDATE 2019-08-16T13:45:53Z External Id SAMEA5870257 INSDC center name UCL Cancer Institute and Institute of Immunity and Transplantation INSDC first public 2020-01-14T04:04:50Z INSDC last update 2019-08-16T13:45:53Z INSDC status public Submitter Id E-MTAB-8255:FRC_untransplanted_55 age 10 broker name ArrayExpress common name house mouse developmental stage adult disease normal genotype wild type genotype individual 55 organism part lymph node sample name E-MTAB-8255:FRC_untransplanted_55 scientific_name Mus musculus sex male strain C57BL/6