ERX3536226 E-MTAB-8314:E10_K20TMT_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739554 SAMEA5951070 E10_K20TMT_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 10.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536227 E-MTAB-8314:E10_K20TMT_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739555 SAMEA5951071 E10_K20TMT_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 10.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536228 E-MTAB-8314:E10_K20TMT_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739556 SAMEA5951072 E10_K20TMT_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 10.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536229 E-MTAB-8314:E14_K20Ezh2homo_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739557 SAMEA5951073 E14_K20Ezh2homo_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;Ezh2fl/fl;Rosa::RFP ERX3536230 E-MTAB-8314:E14_K20Ezh2homo_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739558 SAMEA5951074 E14_K20Ezh2homo_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;Ezh2fl/fl;Rosa::RFP ERX3536231 E-MTAB-8314:E14_K20Ezh2homo_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739559 SAMEA5951075 E14_K20Ezh2homo_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;Ezh2fl/fl;Rosa::RFP ERX3536232 E-MTAB-8314:E14_K20Ezh2wt_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739560 SAMEA5951076 E14_K20Ezh2wt_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536233 E-MTAB-8314:E14_K20Ezh2wt_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739561 SAMEA5951077 E14_K20Ezh2wt_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536234 E-MTAB-8314:E14_K20Ezh2wt_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739562 SAMEA5951078 E14_K20Ezh2wt_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Krox20::Cre;ROSA::tdTomato ERX3536235 E-MTAB-8314:E14_vPrV_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739563 SAMEA5951079 E14_vPrV_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536236 E-MTAB-8314:E14_vPrV_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739564 SAMEA5951080 E14_vPrV_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536237 E-MTAB-8314:E14_vPrV_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739565 SAMEA5951081 E14_vPrV_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 14.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536244 E-MTAB-8314:E18_vPrV_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739572 SAMEA5951088 E18_vPrV_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536245 E-MTAB-8314:E18_vPrV_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739573 SAMEA5951089 E18_vPrV_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536246 E-MTAB-8314:E18_vPrV_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739574 SAMEA5951090 E18_vPrV_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536247 E-MTAB-8314:E18_vPrVKir_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739575 SAMEA5951091 E18_vPrVKir_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa:Kir2.1;r2::EGFP ERX3536248 E-MTAB-8314:E18_vPrVKir_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739576 SAMEA5951092 E18_vPrVKir_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa:Kir2.1;r2::EGFP ERX3536249 E-MTAB-8314:E18_vPrVKir_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739577 SAMEA5951093 E18_vPrVKir_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage embryonic day 18.5 Experimental Factor: genotype Drg11::Cre;Rosa:Kir2.1;r2::EGFP ERX3536250 E-MTAB-8314:P4_vPrV_RNA_1_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739578 SAMEA5951094 P4_vPrV_RNA_1_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage neonate Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536251 E-MTAB-8314:P4_vPrV_RNA_2_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739579 SAMEA5951095 P4_vPrV_RNA_2_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage neonate Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry ERX3536252 E-MTAB-8314:P4_vPrV_RNA_3_s ERP117263 PRJEB34371 RNA-seq of Barrelette neurons and progenitors during mouse brain development ERS3739580 SAMEA5951096 P4_vPrV_RNA_3_s RNA-Seq TRANSCRIPTOMIC PolyA Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C). Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol. Sequencing of poly A+ mRNA was done by Smart-seq2 protocol. 1ng of total RNA was used as an input. Reverse transcription was conducted using 100U SuperScript Ⅱ reverse transcriptase (Thermo Fisher, 18064014), 10U RNase inhibitor (Clontech, 2313A), 1X Superscript Ⅱ first-strand buffer, 5mM DTT (contained in SuperScript Ⅱ reverse transcriptase), 1M Betaine (Sigma, B0300-1VL), 6mM MgCl2, 1μM template-switching oligos (TSOs) (exiqon), 1μM oligo-dT primer (Mycrosynth) and 1mM dNTP mix (Thermo Fisher, R0191) in total volume 10μl. Then PCR pre-amplification was done using 1X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602), 0.1μM IS PCR primers (Mycrosynth) in total volume 25μl using 13 cycles of PCR. DNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 0.8:1) and eluted in 20μl 10mM Tris HCl pH8. Tagmentation reaction was done by Illumina Nextera XT DNA Library Prep Kit (Illumina, FC-131-1024) in total volume 5μl using 0.2ng DNA as an input, then amplification of adapter-ligated fragment was done using Illumina XT kits in a total volume 12.5μl with 12 cycles of PCR. Library was purified by SPRI AMPure XP beads (sample to beads ratio 0.6:1) and eluted in 12μl 10mM Tris HCl pH8. Illumina HiSeq 2500 Experimental Factor: developmental stage neonate Experimental Factor: genotype Drg11::Cre;Rosa::ZsGreen;r2::mCherry