ERP117479 PRJEB34555 Pilot_Sequencing_of_Multiplexed_gRNA_Library-sc-5961 Pilot_Sequencing_of_Multiplexed_gRNA_Library-sc-5961 Pilot_Sequencing_of_Multiplexed_gRNA_Library We have developed a minimised guide RNA library that allows a 3-fold reduction in size and cost of genome-wide CRISPR-Cas9 screen, within the framework of a larger project aimed to understand to which extent gene essentiality varies in healthy humans. This new minimised libraries required the development of a new protocol for the sequencing of the amplicon from genomic DNA. The aim of this pilot is to verify that the new protocol performs well and is scalable. We have developed a CRISPR/Cas9 platform for multiplexed analysis of gene essentiality in human cells. This is a pilot experiment aimed to verify that our current protocol can be scaled up effectively, for future upscaling. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ ENA-FIRST-PUBLIC 2020-10-07 ENA-LAST-UPDATE 2019-09-24