ERS3963211 SAMEA6163205 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163205 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S01 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 1 organism part colon sample name E-MTAB-8500:S01 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963212 SAMEA6163206 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163206 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S02 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 1 organism part colon sample name E-MTAB-8500:S02 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963213 SAMEA6163207 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163207 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S03 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 2 organism part colon sample name E-MTAB-8500:S03 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963214 SAMEA6163208 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163208 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S04 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 2 organism part colon sample name E-MTAB-8500:S04 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963215 SAMEA6163209 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163209 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S05 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 3 organism part colon sample name E-MTAB-8500:S05 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963216 SAMEA6163210 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163210 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S06 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 3 organism part colon sample name E-MTAB-8500:S06 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963217 SAMEA6163211 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163211 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S07 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 4 organism part colon sample name E-MTAB-8500:S07 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963218 SAMEA6163212 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163212 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S08 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype wild type genotype individual 4 organism part colon sample name E-MTAB-8500:S08 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963219 SAMEA6163213 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163213 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S09 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 1 organism part colon sample name E-MTAB-8500:S09 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963220 SAMEA6163214 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163214 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S10 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 1 organism part colon sample name E-MTAB-8500:S10 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963221 SAMEA6163215 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163215 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S11 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 2 organism part colon sample name E-MTAB-8500:S11 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963222 SAMEA6163216 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163216 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S12 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 2 organism part colon sample name E-MTAB-8500:S12 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963223 SAMEA6163217 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163217 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S13 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 3 organism part colon sample name E-MTAB-8500:S13 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963224 SAMEA6163218 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163218 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S14 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 3 organism part colon sample name E-MTAB-8500:S14 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963225 SAMEA6163219 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163219 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S15 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 4 organism part colon sample name E-MTAB-8500:S15 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963226 SAMEA6163220 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163220 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S16 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype C3aRko individual 4 organism part colon sample name E-MTAB-8500:S16 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963227 SAMEA6163221 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163221 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S17 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 1 organism part colon sample name E-MTAB-8500:S17 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963228 SAMEA6163222 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163222 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S18 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 1 organism part colon sample name E-MTAB-8500:S18 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963229 SAMEA6163223 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163223 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S19 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 2 organism part colon sample name E-MTAB-8500:S19 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963230 SAMEA6163224 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163224 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S20 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 2 organism part colon sample name E-MTAB-8500:S20 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963231 SAMEA6163225 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163225 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S21 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual mixed 1 and 2 organism part colon sample name E-MTAB-8500:S21 sampling site neoplastic polyp scientific_name Mus musculus sex female strain C57BL/6J ERS3963232 SAMEA6163226 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163226 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S22 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 3 organism part colon sample name E-MTAB-8500:S22 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963233 SAMEA6163227 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163227 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S23 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 3 organism part colon sample name E-MTAB-8500:S23 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963234 SAMEA6163228 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163228 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S24 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 4 organism part colon sample name E-MTAB-8500:S24 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963235 SAMEA6163229 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163229 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S25 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 4 organism part colon sample name E-MTAB-8500:S25 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963236 SAMEA6163230 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163230 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S26 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 1 organism part colon sample name E-MTAB-8500:S26 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963237 SAMEA6163231 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163231 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S27 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 1 organism part colon sample name E-MTAB-8500:S27 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963238 SAMEA6163232 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163232 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S28 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 1 organism part colon sample name E-MTAB-8500:S28 sampling site neoplastic polyp scientific_name Mus musculus sex male strain C57BL/6J ERS3963239 SAMEA6163233 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163233 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S29 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 2 organism part colon sample name E-MTAB-8500:S29 sampling site proximal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963240 SAMEA6163234 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163234 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S30 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 2 organism part colon sample name E-MTAB-8500:S30 sampling site distal colon scientific_name Mus musculus sex male strain C57BL/6J ERS3963241 SAMEA6163235 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163235 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S31 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 2 organism part colon sample name E-MTAB-8500:S31 sampling site neoplastic polyp scientific_name Mus musculus sex male strain C57BL/6J ERS3963242 SAMEA6163236 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163236 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S32 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 3 organism part colon sample name E-MTAB-8500:S32 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963243 SAMEA6163237 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163237 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S33 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 3 organism part colon sample name E-MTAB-8500:S33 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963244 SAMEA6163238 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163238 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S34 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 3 organism part colon sample name E-MTAB-8500:S34 sampling site neoplastic polyp scientific_name Mus musculus sex female strain C57BL/6J ERS3963245 SAMEA6163239 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163239 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S35 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 4 organism part colon sample name E-MTAB-8500:S35 sampling site proximal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963246 SAMEA6163240 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163240 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S36 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 4 organism part colon sample name E-MTAB-8500:S36 sampling site distal colon scientific_name Mus musculus sex female strain C57BL/6J ERS3963247 SAMEA6163241 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163241 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S37 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin/C3aRko individual 4 organism part colon sample name E-MTAB-8500:S37 sampling site neoplastic polyp scientific_name Mus musculus sex female strain C57BL/6J ERS3963248 SAMEA6163242 10090 Mus musculus Protocols: C57BL/6J-ApcMin/J (referred to as APCMin/+) and C57BL/6J (referred to as WT) mice were bred and maintained in our SPF (Specific Pathogen Free) animal facility. APCMin/+/C3aR-/- mice were generated by crossing C57BL/6J-ApcMin/J with C3aR-/- mice in our animal facility. 12 week old APCMin/+, WT, C3aR-/- and APCMin/+/C3aR-/- mice (4mice/group) were sacrificed. The proximal (first 3 cm closer to the caecum) and distal colon (the final 3,5 cm closer to the rectum) and the single tumors were immediately preserved in 2ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at -20°C. For RNA extraction, tumors and colons were homogenized in Trizol (500 ul/50 mg weight), centrifuged at 13000 rpm and the supernatant was loaded on Qiagen Mini Kit columns and treated as indicated in the manufacturer instructions. RNA concentration was quantified by Nanodrop and quality and integrity were evaluated by using a bioanalyzer (Agilent Technologies). Only samples with a RIN>8 were used for downstream applications. 1ug of RNA was used to prepare cDNA with TruSeq RNA kit by following the recommendations for low sample preparation protocol (Illumina) ENA-FIRST-PUBLIC 2020-02-01T04:03:03Z ENA-LAST-UPDATE 2019-11-07T17:25:26Z External Id SAMEA6163242 INSDC center name UZH INSDC first public 2020-02-01T04:03:03Z INSDC last update 2019-11-07T17:25:26Z INSDC status public Submitter Id E-MTAB-8500:S38 age 12 broker name ArrayExpress common name house mouse developmental stage adult genotype APCMin individual 4 organism part colon sample name E-MTAB-8500:S38 sampling site neoplastic polyp scientific_name Mus musculus sex male strain C57BL/6J