ERS4382858 SAMEA6619127 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:15Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619127 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:15Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_GF_1 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 4 organism part spleen sample name E-MTAB-8883:DC1_GF_1 scientific_name Mus musculus sex female strain germ free C56BL/6 ERS4382859 SAMEA6619128 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:15Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619128 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:15Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_GF_2 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 5 organism part spleen sample name E-MTAB-8883:DC1_GF_2 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382860 SAMEA6619129 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:15Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619129 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:15Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_GF_3 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 6 organism part spleen sample name E-MTAB-8883:DC1_GF_3 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382861 SAMEA6619130 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619130 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_GF_4 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 7 organism part spleen sample name E-MTAB-8883:DC1_GF_4 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382862 SAMEA6619131 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619131 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_IFNAR_1 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 8 organism part spleen sample name E-MTAB-8883:DC1_IFNAR_1 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382863 SAMEA6619132 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619132 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_IFNAR_2 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 9 organism part spleen sample name E-MTAB-8883:DC1_IFNAR_2 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382864 SAMEA6619133 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619133 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_IFNAR_3 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 10 organism part spleen sample name E-MTAB-8883:DC1_IFNAR_3 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382865 SAMEA6619134 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619134 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_IFNAR_4 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 11 organism part spleen sample name E-MTAB-8883:DC1_IFNAR_4 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382866 SAMEA6619135 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619135 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_SPF_1 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 1 organism part spleen sample name E-MTAB-8883:DC1_SPF_1 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382867 SAMEA6619136 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619136 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_SPF_2 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 2 organism part spleen sample name E-MTAB-8883:DC1_SPF_2 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382868 SAMEA6619137 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619137 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC1_SPF_3 age 10 broker name ArrayExpress cell type CD103-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 3 organism part spleen sample name E-MTAB-8883:DC1_SPF_3 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382869 SAMEA6619138 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619138 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_GF_1 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 16 organism part spleen sample name E-MTAB-8883:DC2_GF_1 scientific_name Mus musculus sex female strain germ free C56BL/6 ERS4382870 SAMEA6619139 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619139 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_GF_2 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 17 organism part spleen sample name E-MTAB-8883:DC2_GF_2 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382871 SAMEA6619140 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619140 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_GF_3 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 18 organism part spleen sample name E-MTAB-8883:DC2_GF_3 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382872 SAMEA6619141 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619141 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_GF_4 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 19 organism part spleen sample name E-MTAB-8883:DC2_GF_4 scientific_name Mus musculus sex male strain germ free C56BL/6 ERS4382873 SAMEA6619142 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619142 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_IFNAR_1 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 20 organism part spleen sample name E-MTAB-8883:DC2_IFNAR_1 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382874 SAMEA6619143 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619143 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_IFNAR_2 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 21 organism part spleen sample name E-MTAB-8883:DC2_IFNAR_2 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382875 SAMEA6619144 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619144 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_IFNAR_3 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 22 organism part spleen sample name E-MTAB-8883:DC2_IFNAR_3 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382876 SAMEA6619145 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619145 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_IFNAR_4 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype Ifnar1 knock out individual 23 organism part spleen sample name E-MTAB-8883:DC2_IFNAR_4 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382877 SAMEA6619146 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619146 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_SPF_1 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 12 organism part spleen sample name E-MTAB-8883:DC2_SPF_1 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6 ERS4382878 SAMEA6619147 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619147 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_SPF_2 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 13 organism part spleen sample name E-MTAB-8883:DC2_SPF_2 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382879 SAMEA6619148 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619148 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_SPF_3 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 14 organism part spleen sample name E-MTAB-8883:DC2_SPF_3 scientific_name Mus musculus sex male strain specific pathogen free C56BL/6 ERS4382880 SAMEA6619149 10090 Mus musculus Protocols: Cell were sorted and lysed in Trizol. Total RNA was isolated from Trizol in combination with the miRNeasy Micro Kit (Qiagen). For poly-A-dependent cDNA synthesis and a first amplification step 10 ng of total RNA was used as input in the Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and processed according to the manufacturer's instructions. 1 ng of the purified cDNA was used for tagmentation and library completion with the Nextera XT library preparation kit (Illumina). ENA-FIRST-PUBLIC 2020-04-14T16:26:16Z ENA-LAST-UPDATE 2020-03-10T18:50:51Z External Id SAMEA6619149 INSDC center name Institute of Microbiology, Infectious Diseases and Immunology Charite University Medical Centre Berlin Campus Benjamin Franklin INSDC first public 2020-04-14T16:26:16Z INSDC last update 2020-03-10T18:50:51Z INSDC status public Submitter Id E-MTAB-8883:DC2_SPF_4 age 10 broker name ArrayExpress cell type CD11b-positive dendritic cell common name house mouse developmental stage adult genotype wild type genotype individual 15 organism part spleen sample name E-MTAB-8883:DC2_SPF_4 scientific_name Mus musculus sex female strain specific pathogen free C56BL/6