SRS8946895 SAMEA6675619 3476 Parasponia andersonii Reverse genetics using CRISPR-Cas9 in the tropical tree Parasponia andersonii revealed a promotive role for PanNODULE ROOT1 in stem secondary growth ENA first public 2021-05-03 ENA last update 2020-04-14 External Id SAMEA6675619 INSDC center alias WAGENINGEN UNIVERSITY (Bioinformatics) INSDC center name WAGENINGEN UNIVERSITY (Bioinformatics) INSDC first public 2021-05-03T00:24:50Z INSDC last update 2020-04-14T15:32:08Z INSDC status public SRA accession ERS4425155 Sample Name parasponia_andersonii12 Submitter Id parasponia_andersonii12 air temperature regimen 28degrees collection date 2019 culture rooting medium SH salts 3.2 g.L-1, and SH vitamins 1 g.L-1 estimated size 550mb genetic modification CRISPR-Cas9 knockout of PanNOOT1 genotype PanNOOT1_A10 geographic location (country and/or sea) Netherlands geographic location (latitude) 51.9669161323 geographic location (longitude) 5.65515904602 growth facility growth chamber light regimen 16/8h day/night mineral nutrient regimen nutritive solution (NH4, 1 mmol.L-1; K, 5.9 mmol.L-1; Ca; 2.7 mmol.L-1; Mg, 0.8 mmol.L-1; NO3, 9 mmol.L-1; SO4, 1.9 mmol.L-1; P, 1.1 mmol.L-1; Fe, 15 µmol.L-1; Mn, 5 µmol.L-1; Zn, 5 µmol.L-1; B, 3 µmol.L-1; Cu, 0.5 µmol.L-1; Mo, 0.5 µmol.L-1; pH: 5.8) plant developmental stage stem internode 4 plant growth medium Schenk & Hildebrandt (SH) plant structure stem ploidy 2n rooting medium carbon sucrose 10 g.L-1 rooting medium pH 5.8 rooting medium regulators IBA 1 µg.mL-1; NAA 0.1 µg.mL-1; MES 3 mM rooting medium solidifier Daishin agar sample health state healthy title Reverse genetics using CRISPR-Cas9 in the tropical tree Parasponia andersonii revealed a promotive role for PanNODULE ROOT1 in stem secondary growth watering regimen 2times/week water or nutritive solution