ERS16172562 SAMEA114191866 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 1 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L0 organism part brain sample name E-MTAB-13161:Sample 1 scientific_name Homo sapiens ERS16172563 SAMEA114191867 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 2 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L1 organism part brain sample name E-MTAB-13161:Sample 2 scientific_name Homo sapiens ERS16172564 SAMEA114191868 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 3 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L2 organism part brain sample name E-MTAB-13161:Sample 3 scientific_name Homo sapiens ERS16172565 SAMEA114191869 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 4 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L0 organism part brain sample name E-MTAB-13161:Sample 4 scientific_name Homo sapiens ERS16172566 SAMEA114191870 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 5 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L0 organism part brain sample name E-MTAB-13161:Sample 5 scientific_name Homo sapiens ERS16172567 SAMEA114191871 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 6 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L1 organism part brain sample name E-MTAB-13161:Sample 6 scientific_name Homo sapiens ERS16172568 SAMEA114191872 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 7 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L1 organism part brain sample name E-MTAB-13161:Sample 7 scientific_name Homo sapiens ERS16172569 SAMEA114191873 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 8 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L2 organism part brain sample name E-MTAB-13161:Sample 8 scientific_name Homo sapiens ERS16172570 SAMEA114191874 9606 Homo sapiens Protocols: Cultured human glioblastoma cell pellets were snap frozen until RNA extraction. Human glioblastoma cells were grown in N2 medium containing 20 ng/ml recombinant human EGF and 2% bovine serum albumin. Prior to RNA extraction, human glioblastoma cells were treated with 20 ng/ml recombinant human FGF2 for 48 hours. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers instructions. Library preparation for RNA sequencing was performed using the Ion Total RNA-Seq V2 kit and Ion Xpress RNA-Seq Barcode kit (Thermo Fisher). INSDC center name Cardiff University School of Biosciences European Cancer Stem Cell Research Institute Submitter Id E-MTAB-13161:Sample 9 broker name ArrayExpress cell_line hGBM cell_type astrocyte collection date not collected common name human developmental stage adult disease glioblastoma multiforme genotype wild type genotype geographic location (country and/or sea) not collected isolate not applicable line L2 organism part brain sample name E-MTAB-13161:Sample 9 scientific_name Homo sapiens