ERX11157328 E-MTAB-13187:Sample 10_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172588 SAMEA114191892 Sample 10_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157329 E-MTAB-13187:Sample 11_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172589 SAMEA114191893 Sample 11_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157330 E-MTAB-13187:Sample 12_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172590 SAMEA114191894 Sample 12_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157331 E-MTAB-13187:Sample 16_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172591 SAMEA114191895 Sample 16_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157332 E-MTAB-13187:Sample 17_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172592 SAMEA114191896 Sample 17_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157333 E-MTAB-13187:Sample 18_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172593 SAMEA114191897 Sample 18_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157334 E-MTAB-13187:Sample 22_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172594 SAMEA114191898 Sample 22_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157335 E-MTAB-13187:Sample 23_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172595 SAMEA114191899 Sample 23_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157336 E-MTAB-13187:Sample 24_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172596 SAMEA114191900 Sample 24_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157337 E-MTAB-13187:Sample 4_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172597 SAMEA114191901 Sample 4_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157338 E-MTAB-13187:Sample 5_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172598 SAMEA114191902 Sample 5_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157339 E-MTAB-13187:Sample 6_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172599 SAMEA114191903 Sample 6_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157340 E-MTAB-13187:Sample 1_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172600 SAMEA114191904 Sample 1_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157341 E-MTAB-13187:Sample 13_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172601 SAMEA114191905 Sample 13_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157342 E-MTAB-13187:Sample 14_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172602 SAMEA114191906 Sample 14_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157343 E-MTAB-13187:Sample 15_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172603 SAMEA114191907 Sample 15_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157344 E-MTAB-13187:Sample 19_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172604 SAMEA114191908 Sample 19_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157345 E-MTAB-13187:Sample 2_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172605 SAMEA114191909 Sample 2_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157346 E-MTAB-13187:Sample 20_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172606 SAMEA114191910 Sample 20_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157347 E-MTAB-13187:Sample 21_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172607 SAMEA114191911 Sample 21_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157348 E-MTAB-13187:Sample 3_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172608 SAMEA114191912 Sample 3_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157349 E-MTAB-13187:Sample 7_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172609 SAMEA114191913 Sample 7_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157350 E-MTAB-13187:Sample 8_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172610 SAMEA114191914 Sample 8_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION ERX11157351 E-MTAB-13187:Sample 9_s ERP149731 PRJEB64552 Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+) ERS16172611 SAMEA114191915 Sample 9_s RNA-Seq TRANSCRIPTOMIC other Total RNA was extracted from cells after treatment with or without NAD+ (3 biological replicates per cell line per condition). Cells were treated for 24 hours with 2 mM NAD+. Cells were untreated as the control condition. Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions, including QIAshredder spin columns for homogenization. Total RNA was used as input with the library preparation SQK-PCB109 kit (Oxford Nanopore Technologies, ONT) according to manufacturer's instructions. Two libraries were prepared: one with HeLa and U2OS samples, and one with BJ and BJ-5ta samples. In the barcoding-PCR step, 14 cycles with 4 min extension time were performed. Barcoded cDNA samples were pooled into a library with a final mass of 100 ng. MinION