ERS16172649 SAMEA114191953 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191953 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:MK288_A broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype wild type phenotype sample name E-MTAB-13195:MK288_A scientific_name Saccharomyces cerevisiae strain MK288 ERS16172650 SAMEA114191954 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191954 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:MK288_B broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype wild type phenotype sample name E-MTAB-13195:MK288_B scientific_name Saccharomyces cerevisiae strain MK288 ERS16172651 SAMEA114191955 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191955 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:MK288_C broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype wild type phenotype sample name E-MTAB-13195:MK288_C scientific_name Saccharomyces cerevisiae strain MK288 ERS16172652 SAMEA114191956 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191956 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ140_A broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ140_A scientific_name Saccharomyces cerevisiae strain TJ140 ERS16172653 SAMEA114191957 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191957 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ140_B broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ140_B scientific_name Saccharomyces cerevisiae strain TJ140 ERS16172654 SAMEA114191958 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191958 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ140_C broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ140_C scientific_name Saccharomyces cerevisiae strain TJ140 ERS16172655 SAMEA114191959 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191959 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ235_A broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ235_A scientific_name Saccharomyces cerevisiae strain TJ235 ERS16172656 SAMEA114191960 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191960 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ235_B broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ235_B scientific_name Saccharomyces cerevisiae strain TJ235 ERS16172657 SAMEA114191961 4932 Saccharomyces cerevisiae Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) buffer at appropriate temperature. Then First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end repair. The cDNA fragments obtained from previous step were amplified by PCR, and products were purified by Ampure XP Beads, then dissolved in EB solution. The product was validated on the Agilent Technologies 2100 bioanalyzer for quality control. The double stranded PCR products from previous step were heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA (ssCir DNA) was formatted as the final library. ENA-FIRST-PUBLIC 2023-07-24T16:16:09Z ENA-LAST-UPDATE 2023-07-24T16:16:09Z External Id SAMEA114191961 INSDC center alias Shanghai Tenth People's Hospital INSDC center name Shanghai Tenth People's Hospital INSDC first public 2023-07-24T16:16:09Z INSDC last update 2023-07-24T16:16:09Z INSDC status public Submitter Id E-MTAB-13195:TJ235_C broker name ArrayExpress collection date not collected common name baker's yeast developmental stage log phase genotype wild type genotype geographic location (country and/or sea) not collected growth condition YPD broth isolate not applicable organism part whole organism phenotype petite sample name E-MTAB-13195:TJ235_C scientific_name Saccharomyces cerevisiae strain TJ235