ERS362599 SAMEA2225914 9606 Homo sapiens Protocols: MCF7 cells were cultured in RPMI1640 medium (GIBCO/BRL) supplemented with 10% (v/v) fetal bovine serum (GIBCO/BRL), 2.0 g/L sodium bicarbonate, and were incubated in a humidified 37C incubator with 5% CO2. MCF7 cells were grown to 85% confluence in 6 cm tissue culture dish. Each 6 cm dish was rinsed twice with 1X PBS. Total RNA was extracted using TRIreagent (Invitrogen) protocol. The integrity of the RNA extract was checked by 1.2% (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry. The normal breast tissue mRNA were purchased from Origene (Catalog No.: CR559104 and CR561898). The sequencing library for mRNA-seq was prepared using TruSeq RNA Sample Preparation Kit (Illumina Inc., USA) as per manufacturers instruction. Briefly, total RNA with RIN (RNA integrity number) greater than 7.5 was subjected for poly-A mRNA isolation using poly-T oligo-attached magnetic beads. The poly-A mRNA was fragmented and first-strand cDNA was synthesized using random hexamers following by second-strand cDNA synthesis, end repair, addition of a single A base and adapter ligation. The adapter-ligated cDNA library was sized-selected by agarose gel and amplified by PCR. ENA-FIRST-PUBLIC 2015-02-25T17:03:15Z ENA-LAST-UPDATE 2018-03-08T16:50:35Z External Id SAMEA2225914 INSDC center name VGH-YM Genome Research Center INSDC first public 2015-02-25T17:03:15Z INSDC last update 2018-03-08T16:50:35Z INSDC status public Submitter Id E-MTAB-1961:MCF7 cells broker name ArrayExpress cell line MCF-7 common name human disease breast adenocarcinoma sample name E-MTAB-1961:MCF7 cells scientific_name Homo sapiens sex female ERS362600 SAMEA2225915 9606 Homo sapiens Protocols: MCF7 cells were cultured in RPMI1640 medium (GIBCO/BRL) supplemented with 10% (v/v) fetal bovine serum (GIBCO/BRL), 2.0 g/L sodium bicarbonate, and were incubated in a humidified 37C incubator with 5% CO2. MCF7 cells were grown to 85% confluence in 6 cm tissue culture dish. Each 6 cm dish was rinsed twice with 1X PBS. Total RNA was extracted using TRIreagent (Invitrogen) protocol. The integrity of the RNA extract was checked by 1.2% (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry. The normal breast tissue mRNA were purchased from Origene (Catalog No.: CR559104 and CR561898). The sequencing library for mRNA-seq was prepared using TruSeq RNA Sample Preparation Kit (Illumina Inc., USA) as per manufacturers instruction. Briefly, total RNA with RIN (RNA integrity number) greater than 7.5 was subjected for poly-A mRNA isolation using poly-T oligo-attached magnetic beads. The poly-A mRNA was fragmented and first-strand cDNA was synthesized using random hexamers following by second-strand cDNA synthesis, end repair, addition of a single A base and adapter ligation. The adapter-ligated cDNA library was sized-selected by agarose gel and amplified by PCR. ENA-FIRST-PUBLIC 2015-02-25T17:03:15Z ENA-LAST-UPDATE 2018-03-08T16:50:35Z External Id SAMEA2225915 INSDC center name VGH-YM Genome Research Center INSDC first public 2015-02-25T17:03:15Z INSDC last update 2018-03-08T16:50:35Z INSDC status public Submitter Id E-MTAB-1961:CR559104-FR0001BA18 broker name ArrayExpress common name human disease normal sample name E-MTAB-1961:CR559104-FR0001BA18 scientific_name Homo sapiens sex female ERS362601 SAMEA2225916 9606 Homo sapiens Protocols: MCF7 cells were cultured in RPMI1640 medium (GIBCO/BRL) supplemented with 10% (v/v) fetal bovine serum (GIBCO/BRL), 2.0 g/L sodium bicarbonate, and were incubated in a humidified 37C incubator with 5% CO2. MCF7 cells were grown to 85% confluence in 6 cm tissue culture dish. Each 6 cm dish was rinsed twice with 1X PBS. Total RNA was extracted using TRIreagent (Invitrogen) protocol. The integrity of the RNA extract was checked by 1.2% (w/v) agarose gel electrophoresis and the concentration of RNA was estimated by ultraviolet spectrophotometry. The normal breast tissue mRNA were purchased from Origene (Catalog No.: CR559104 and CR561898). The sequencing library for mRNA-seq was prepared using TruSeq RNA Sample Preparation Kit (Illumina Inc., USA) as per manufacturers instruction. Briefly, total RNA with RIN (RNA integrity number) greater than 7.5 was subjected for poly-A mRNA isolation using poly-T oligo-attached magnetic beads. The poly-A mRNA was fragmented and first-strand cDNA was synthesized using random hexamers following by second-strand cDNA synthesis, end repair, addition of a single A base and adapter ligation. The adapter-ligated cDNA library was sized-selected by agarose gel and amplified by PCR. ENA-FIRST-PUBLIC 2015-02-25T17:03:15Z ENA-LAST-UPDATE 2018-03-08T16:50:35Z External Id SAMEA2225916 INSDC center name VGH-YM Genome Research Center INSDC first public 2015-02-25T17:03:15Z INSDC last update 2018-03-08T16:50:35Z INSDC status public Submitter Id E-MTAB-1961:CR561898-FR0042A6F broker name ArrayExpress common name human disease normal sample name E-MTAB-1961:CR561898-FR0042A6F scientific_name Homo sapiens sex female