ERS16286482 SAMEA114297375 1891229 Pseudomonadales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 68f9a3b9-fb3d-4940-833e-472fe0396233 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 94.07 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 6.71 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name 68f9a3b9-fb3d-4940-833e-472fe0396233 scientific_name Pseudomonadales bacterium sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker ANI tolid kaTriMini1.Pseudomonadales_1 ERS16286483 SAMEA114297376 259942 uncultured Prochloron sp. 16S recovered No 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 413ac2c4-bbad-4dca-8f4a-cb6243bb5951 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 76.67 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.55 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 33 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name 413ac2c4-bbad-4dca-8f4a-cb6243bb5951 scientific_name uncultured Prochloron sp. sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker taxonomic classification defined by topology and ANI tolid kaTriMini1.Prochloron_sp_1 ERS16286484 SAMEA114297377 1913961 Rhizobiaceae bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id a795812a-e626-4e9b-a210-70d0cb90972c assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 72.93 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 5.11 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 35 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name a795812a-e626-4e9b-a210-70d0cb90972c scientific_name Rhizobiaceae bacterium sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker taxonomic classification defined by topology and ANI tolid kaTriMini1.Rhizobiaceae_1 ERS16286485 SAMEA114297378 293344 uncultured Roseovarius sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id fde22e01-18c0-43d8-9862-eb9c591a2007 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 97.51 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.43 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 43 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name fde22e01-18c0-43d8-9862-eb9c591a2007 scientific_name uncultured Roseovarius sp. sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker ANI tolid kaTriMini1.Roseovarius_sp_1 ERS16286486 SAMEA114297379 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id f2056530-3023-4fc6-a5f8-b9ce68973d47 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 92.43 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.53 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 41 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name f2056530-3023-4fc6-a5f8-b9ce68973d47 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker taxonomic novelty determined using RED tolid kaTriMini1.Gammaproteobacteria_1 ERS16286487 SAMEA114297380 191468 uncultured Sulfitobacter sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id c7aa7673-099f-424b-b76d-a1e9a7826d04 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 95.45 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.55 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name c7aa7673-099f-424b-b76d-a1e9a7826d04 scientific_name uncultured Sulfitobacter sp. sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker taxonomic classification defined by topology and ANI tolid kaTriMini1.Sulfitobacter_sp_1 ERS16286488 SAMEA114297381 2202144 Spirochaetota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) GAL Nova Southeastern University GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3225296a-4e2d-4851-a7a3-e3bf13ad47d1 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected by EUICHI HIROSE collecting institution UNIVERSITY OF THE RYUKYUS collection date 2021-03-15 completeness score 94.93 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 0.5 environmental medium adult tunicate tissue geographic location (country and/or sea) Japan geographic location (latitude) 26.70833333 geographic location (longitude) 127.8777778 geographic location (region and locality) Okinawa|Motobu|Back Reef at Bise habitat Rubbles of dead coral branches host life stage adult host scientific name Trididemnum miniatum host sex hermaphrodite host taxid 1128088 identified by EUICHI HIROSE identifier_affiliation UNIVERSITY OF THE RYUKYUS investigation type metagenome-assembled genome isolation_source Tunicate: Trididemnum miniatum local environmental context marine benthic biome metagenomic source sea squirt metagenome number of standard tRNAs extracted 35 organism part WHOLE ORGANISM project name ASG relationship obligate symbiosis at least for host sample derived from SAMEA114297374 sample name 3225296a-4e2d-4851-a7a3-e3bf13ad47d1 scientific_name Spirochaetota bacterium sequencing method Pacbio Sequel II specimen id NSU0014201 specimen voucher 3 tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.1.1); GTDB (release 207_v2) taxonomic identity marker taxonomic classification defined by topology and ANI tolid kaTriMini1.Spirochaetota_1