ERX11274105 E-MTAB-13292:Ble-3_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288860 SAMEA114299636 Ble-3_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274111 E-MTAB-13292:WT-3_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288866 SAMEA114299642 WT-3_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274103 E-MTAB-13292:Ble-1_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288858 SAMEA114299634 Ble-1_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274109 E-MTAB-13292:WT-1_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288864 SAMEA114299640 WT-1_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274110 E-MTAB-13292:WT-2_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288865 SAMEA114299641 WT-2_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274104 E-MTAB-13292:Ble-2_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288859 SAMEA114299635 Ble-2_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274107 E-MTAB-13292:DPRs-2_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288862 SAMEA114299638 DPRs-2_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274108 E-MTAB-13292:DPRs-3_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288863 SAMEA114299639 DPRs-3_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000 ERX11274106 E-MTAB-13292:DPRs-1_s ERP150493 PRJEB65357 RNA seq of HEK293 treated with Blebbistatin or expressing C9orf72 associated Di Peptide Repeats (DPRs) ERS16288861 SAMEA114299637 DPRs-1_s RNA-Seq TRANSCRIPTOMIC Oligo-dT Samples were lysed on plate using RNeasy kit (Qiagen) Lysis buffer. RNA was extracted using RNeasy kit (Qiagen) using manufacturer protocol. DNA was removed in column treatment and in-solution with DNAse I. Individual libraries were generated from 500ng of each total RNA sample using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina (Lexogen Inc, #015). Libraries were prepared according to the QuantSeq 3' mRNA-seq Library Prep Kit (FWD) protocol. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts. Library generation was initiated by oligo-dT priming. The primer already contains Illumina-compatible linker sequences. After first strand synthesis, the RNA was removed and second strand synthesis initiated by random priming and a DNA polymerase. With the QuantSeq FWD kit the Read 1 linker sequence is contained in the second strand synthesis primer so NGS reads are generated towards the poly (A) tail and directly correspond to the mRNA sequence. Second strand synthesis was followed by a magnetic bead-based purification step before cDNA libraries were amplified for 13 cycles introducing the sequences required for cluster generation and index sequences to allow multiplexing of sequencing libraries. After amplification a final bead-purification removed any excess primers or adapterdimers that may interfere with sequencing. NextSeq 2000