ERS4993307 SAMEA7233331 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:07Z External Id SAMEA7233331 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:07Z INSDC status public Submitter Id E-MTAB-9482:mock_VH10_I2L age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH10_I2L scientific_name Mus musculus sex male strain CD-1 ERS4993308 SAMEA7233332 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:07Z External Id SAMEA7233332 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:07Z INSDC status public Submitter Id E-MTAB-9482:mock_VH1_IL age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH1_IL scientific_name Mus musculus sex male strain CD-1 ERS4993309 SAMEA7233333 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233333 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH3_I2L2R age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH3_I2L2R scientific_name Mus musculus sex male strain CD-1 ERS4993310 SAMEA7233334 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233334 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH4_II2R age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH4_II2R scientific_name Mus musculus sex male strain CD-1 ERS4993311 SAMEA7233335 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233335 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH5_IIL age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH5_IIL scientific_name Mus musculus sex male strain CD-1 ERS4993312 SAMEA7233336 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233336 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH6_IIR age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH6_IIR scientific_name Mus musculus sex male strain CD-1 ERS4993313 SAMEA7233337 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233337 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH7_IIA2R age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH7_IIA2R scientific_name Mus musculus sex male strain CD-1 ERS4993314 SAMEA7233338 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233338 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH8_IIALR age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH8_IIALR scientific_name Mus musculus sex male strain CD-1 ERS4993315 SAMEA7233339 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233339 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:mock_VH9_IIAL age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:mock_VH9_IIAL scientific_name Mus musculus sex male strain CD-1 ERS4993316 SAMEA7233340 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233340 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:plasmid_VH21 broker name ArrayExpress common name house mouse sample name E-MTAB-9482:plasmid_VH21 scientific_name Mus musculus ERS4993317 SAMEA7233341 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233341 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:pretrt_VH2VH12VH22 broker name ArrayExpress common name house mouse sample name E-MTAB-9482:pretrt_VH2VH12VH22 scientific_name Mus musculus ERS4993318 SAMEA7233342 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233342 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:tax_VH15_IBR age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:tax_VH15_IBR scientific_name Mus musculus sex male strain CD-1 ERS4993319 SAMEA7233343 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233343 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:tax_VH16_IBL age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:tax_VH16_IBL scientific_name Mus musculus sex male strain CD-1 ERS4993321 SAMEA7233345 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233345 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:tax_VH23_IBLR age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:tax_VH23_IBLR scientific_name Mus musculus sex male strain CD-1 ERS4993322 SAMEA7233346 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233346 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:tax_VH24_IB2R age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:tax_VH24_IB2R scientific_name Mus musculus sex male strain CD-1 ERS4993320 SAMEA7233344 10090 Mus musculus Protocols: DNA was extracted from each whole tumour using the Blood and Cell Culture DNA Maxi kit (Qiagen) according to the manufacturer's instructions. DNA was extracted from 100mg of ground tumour (or entire tumour if weight under 100mg) using QIAamp DNA Mini kit (Qiagen) as per the manufacturer's instructions. DNA was prepared for deep sequencing by conducting a two-step PCR. The initial PCR amplified a region of the gRNA cassette to maintain library representation, while the second PCR added the primers required for sequencing. PCR was repeated 35 times for 100 fold library representation. All PCR products per sample were combined and used in the second PCR round to add the primers required for sequencing. PCR products from each sample were again combined and concentrated using a QIAquick PCR Purification Kit (Qiagen) as per the manufacturer's instructions. Each sample was run on a 1.5% agarose gel. Bands were excised and DNA purified using a QIAquick Gel Extraction Kit before sending for sequencing. ENA-FIRST-PUBLIC 2020-10-07T08:09:51Z ENA-LAST-UPDATE 2020-08-26T11:59:08Z External Id SAMEA7233344 INSDC center name Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden, Glasgow, G61 1QH, UK. CRUK Beatson Institute, Bearsden, Glasgow, G61 1BD, UK. INSDC first public 2020-10-07T08:09:51Z INSDC last update 2020-08-26T11:59:08Z INSDC status public Submitter Id E-MTAB-9482:tax_VH17_IB2L age 6 broker name ArrayExpress common name house mouse developmental stage adult disease prostate cancer genotype PbCre Ptenfl/fl Spry2fl/+ organism part prostate gland sample name E-MTAB-9482:tax_VH17_IB2L scientific_name Mus musculus sex male strain CD-1