ERX4488714 E-MTAB-9487:Sample 1_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039486 SAMEA7278883 Sample 1_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus static ERX4488715 E-MTAB-9487:Sample 10_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039487 SAMEA7278884 Sample 10_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus tension ERX4488716 E-MTAB-9487:Sample 11_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039488 SAMEA7278885 Sample 11_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus tension ERX4488717 E-MTAB-9487:Sample 12_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039489 SAMEA7278886 Sample 12_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus tension ERX4488718 E-MTAB-9487:Sample 2_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039490 SAMEA7278887 Sample 2_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus static ERX4488719 E-MTAB-9487:Sample 3_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039491 SAMEA7278888 Sample 3_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus static ERX4488720 E-MTAB-9487:Sample 4_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039492 SAMEA7278889 Sample 4_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus tension ERX4488721 E-MTAB-9487:Sample 5_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039493 SAMEA7278890 Sample 5_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus tension ERX4488722 E-MTAB-9487:Sample 6_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039494 SAMEA7278891 Sample 6_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Mus musculus Experimental Factor: stimulus tension ERX4488725 E-MTAB-9487:Sample 9_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039497 SAMEA7278894 Sample 9_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus static ERX4488723 E-MTAB-9487:Sample 7_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039495 SAMEA7278892 Sample 7_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus static ERX4488724 E-MTAB-9487:Sample 8_s ERP123664 PRJEB40067 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems ERS5039496 SAMEA7278893 Sample 8_s RNA-Seq TRANSCRIPTOMIC RT-PCR Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Illumina NovaSeq 6000 Experimental Factor: organism Homo sapiens Experimental Factor: stimulus static