ERS5039486 SAMEA7278883 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278883 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 1 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 1 scientific_name Mus musculus stimulus static ERS5039487 SAMEA7278884 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278884 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 10 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 10 scientific_name Homo sapiens stimulus tension ERS5039488 SAMEA7278885 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278885 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 11 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 11 scientific_name Homo sapiens stimulus tension ERS5039489 SAMEA7278886 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278886 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 12 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 12 scientific_name Homo sapiens stimulus tension ERS5039490 SAMEA7278887 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278887 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 2 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 2 scientific_name Mus musculus stimulus static ERS5039491 SAMEA7278888 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278888 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 3 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 3 scientific_name Mus musculus stimulus static ERS5039492 SAMEA7278889 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278889 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 4 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 4 scientific_name Mus musculus stimulus tension ERS5039493 SAMEA7278890 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278890 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 5 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 5 scientific_name Mus musculus stimulus tension ERS5039494 SAMEA7278891 10090 Mus musculus Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278891 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 6 broker name ArrayExpress cell line MLO-Y4 cell type osteocyte common name house mouse organism part bone sample name E-MTAB-9487:Sample 6 scientific_name Mus musculus stimulus tension ERS5039495 SAMEA7278892 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278892 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 7 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 7 scientific_name Homo sapiens stimulus static ERS5039496 SAMEA7278893 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278893 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 8 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 8 scientific_name Homo sapiens stimulus static ERS5039497 SAMEA7278894 9606 Homo sapiens Protocols: Immediately after the mechanical loading, cells were washed twice with ice-cold PBS and lysed with RLT buffer (Qiagen) for 1 minute. RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer instructions. RNA quantity and quality were estimated using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™) Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. ENA-FIRST-PUBLIC 2021-01-04T04:05:26Z ENA-LAST-UPDATE 2020-08-27T11:14:00Z External Id SAMEA7278894 INSDC center name NTU INSDC first public 2021-01-04T04:05:26Z INSDC last update 2020-08-27T11:14:00Z INSDC status public Submitter Id E-MTAB-9487:Sample 9 broker name ArrayExpress cell type osteocyte common name human organism part bone sample name E-MTAB-9487:Sample 9 scientific_name Homo sapiens stimulus static