ERP123664 PRJEB40067 E-MTAB-9487 Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems Osteocytes are secretory bone cells that can play an important role in regenerative rehabilitation through their secretome. Making use of RNA-seq and bioinformatics we examined the transcriptome of mouse and human osteocytic bone cells cultured under cyclic tension delivered by a computer-controlled bioreactor. We discovered that a single bout of tensile stretch is enough to activate signalling pathways or processes implicated in bone tissue regeneration, and DNA-repair systems which are relevant to cancer. This results are valuable to the scientific community by allowing to inform future exercise-based regenerative rehabilitation protocols targeting musculoskeletal disorders or cancer. Osteocytes are secretory bone cells that can play an important role in regenerative rehabilitation through their secretome. Making use of RNA-seq and bioinformatics we examined the transcriptome of mouse and human osteocytic bone cells cultured under cyclic tension delivered by a computer-controlled bioreactor. We discovered that a single bout of tensile stretch is enough to activate signalling pathways or processes implicated in bone tissue regeneration, and DNA-repair systems which are relevant to cancer. This results are valuable to the scientific community by allowing to inform future exercise-based regenerative rehabilitation protocols targeting musculoskeletal disorders or cancer. E-MTAB-9487 in ArrayExpress http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-9487 ENA-FIRST-PUBLIC 2021-06-02 ENA-LAST-UPDATE 2021-06-02