ERS17978866 SAMEA115164139 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient6-P1 broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient6 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient6-P1 scientific_name Homo sapiens sex female ERS17978861 SAMEA115164134 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient4-2D broker name ArrayExpress cell_type P2 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient3 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient4-2D scientific_name Homo sapiens sex female ERS17978852 SAMEA115164125 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-P2 broker name ArrayExpress cell_type P2 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-P2 scientific_name Homo sapiens sex female ERS17978865 SAMEA115164138 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient6-F broker name ArrayExpress cell_type F collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient6 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient6-F scientific_name Homo sapiens sex female ERS17978863 SAMEA115164136 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient5-2D broker name ArrayExpress cell_type P1 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient6 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient5-2D scientific_name Homo sapiens sex female ERS17978847 SAMEA115164121 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-2D broker name ArrayExpress cell_type F collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-2D scientific_name Homo sapiens sex female ERS17978864 SAMEA115164137 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient6-2D broker name ArrayExpress cell_type P3 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient6 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient6-2D scientific_name Homo sapiens sex female ERS17978848 SAMEA115164122 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-F-1 broker name ArrayExpress cell_type F collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-F-1 scientific_name Homo sapiens sex female ERS17978857 SAMEA115164130 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient3-2D broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient3 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient3-2D scientific_name Homo sapiens sex female ERS17978853 SAMEA115164126 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-P3 broker name ArrayExpress cell_type P3 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-P3 scientific_name Homo sapiens sex female ERS17978854 SAMEA115164127 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient2-2D broker name ArrayExpress cell_type F collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient2 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient2-2D scientific_name Homo sapiens sex female ERS17978858 SAMEA115164131 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient3-P1 broker name ArrayExpress cell_type P1 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient3 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient3-P1 scientific_name Homo sapiens sex female ERS17978851 SAMEA115164124 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-P1 broker name ArrayExpress cell_type P1 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-P1 scientific_name Homo sapiens sex female ERS17978850 SAMEA115164123 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient1-F-2 broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient1 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient1-F-2 scientific_name Homo sapiens sex female ERS17978862 SAMEA115164135 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient4-F broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient5 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient4-F scientific_name Homo sapiens sex female ERS17978867 SAMEA115164140 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient6-P3 broker name ArrayExpress cell_type P2 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient3 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient6-P3 scientific_name Homo sapiens sex female ERS17978855 SAMEA115164128 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient2-F broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient2 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient2-F scientific_name Homo sapiens sex female ERS17978860 SAMEA115164133 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient3-P2-2 broker name ArrayExpress cell_type 2D collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient4 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient3-P2-2 scientific_name Homo sapiens sex female ERS17978856 SAMEA115164129 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient2-P1 broker name ArrayExpress cell_type P1 collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient2 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient2-P1 scientific_name Homo sapiens sex female ERS17978859 SAMEA115164132 9606 Homo sapiens Protocols: Primary tumor cells at passage 1 were dissociated through trypsin/EDTA (Lonza cat. no. BE17-161F), centrifuged at 300 x g for 5 min, the supernatant was removed and the cell pellet was washed twice with pre-warmed Dulbecco-s Phosphate Buffered Saline (D-PBS 1X) and incubated with 0.25% trypsin/EDTA solution at 37 °C until cells are detached from the bottom of the flask. Cells were collected in D-PBS, pelleted at 300 x g for 5 minutes at RT, suspended in 500l of pre-warmed stem media (MEBM) and counted both with TC-20 automated cell counter (Bio-Rad) and counting chamber (Biosigma-Fast Read 102). The cells were centrifuged at 300 x g for 5 minutes, and the pellet was resuspended in pre warmed serum-free MEBM supplemented with 100U/ml penicillin, 100ug/ml streptomycin, 2mML-glutamine, 5ug/ml insulin, 0.5ug/ml hydrocortisone, 1U/ml heparin,2% B27, 20ug/ml epidermal growth factor, 20ug/ml fibroblast growth factor and diluted by serial limiting dilution at the density of 1 cell for well in the media supplemented with 12.5% of ascitic fluid for plating into low cell adhesion 96 well plates (Sumitomo Bakelite cat. no. MS-9096V 96 well) with a final volume of 200 l for well. sMOCS at day 8-11 was moved from 96-well V-bottom ultra-low attachment plates to 48-well ultra-low attachment plates (Corning), and incubated with 400l of pre-warmed 0.25% trypsin/EDTA solution at 37 °C for 20-30 minutes with gently pipetting for 20 times every 8 minutes and visualized by microscope for the stadium of disaggregation. To avoid loose of cells the tip was always rinsed with medium before pipetting. Cells were harvested, washed once in MEBM plus ascitic fluid and centrifuged at 300 x g for 5 min. Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v2 ENA first public 2024-01-30 INSDC center name Department of Experimental Oncology, European Institute of Oncology IRCCS INSDC status public Submitter Id E-MTAB-11207_2:Patient3-P2-1 broker name ArrayExpress cell_type F collection date not collected common name human disease HGSOC geographic location (country and/or sea) not collected individual Patient4 isolate not applicable organism part ascitic fluid sample name E-MTAB-11207_2:Patient3-P2-1 scientific_name Homo sapiens sex female