ERS398430 SAMEA2340759 10090 Mus musculus Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-IRES-GFP-puro (NS5_control) or pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), the latter sample collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/mL . DNAse-seq samples from NS5 cells were prepared as described by Song et al (Genome Res, 2011). Libraries were generated as in (Boyle et al., 2008; Song and Crawford, 2010) with a slight modification made to the linkers to increase ligation efficiency (Song et al., 2011, Genome Res). ENA-FIRST-PUBLIC 2014-10-02T17:04:02Z ENA-LAST-UPDATE 2018-03-08T17:09:20Z External Id SAMEA2340759 INSDC center name Instituto Gulbenkian de Ciencia INSDC first public 2014-10-02T17:04:02Z INSDC last update 2018-03-08T17:09:20Z INSDC status public Submitter Id E-MTAB-2270:NS5_control broker name ArrayExpress cell line NS5 cell type neuronal stem cell common name house mouse genotype pBABE-IRES-GFP-puro sample name E-MTAB-2270:NS5_control scientific_name Mus musculus ERS398431 SAMEA2340760 10090 Mus musculus Protocols: NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were infected with pBABE-IRES-GFP-puro (NS5_control) or pBABE-Ascl1-ERT2-IRES-GFP-puro (NS5_Ascl1), followed by selection in complete medium containing 1ug/mL puromycin (Calbiochem), the latter sample collected 24 hours after induction with 4-Hydroxytamoxifen at a final concentration of 50nM, while reducing EGF concentration to 5ng/mL . DNAse-seq samples from NS5 cells were prepared as described by Song et al (Genome Res, 2011). Libraries were generated as in (Boyle et al., 2008; Song and Crawford, 2010) with a slight modification made to the linkers to increase ligation efficiency (Song et al., 2011, Genome Res). ENA-FIRST-PUBLIC 2014-10-02T17:04:02Z ENA-LAST-UPDATE 2018-03-08T17:09:20Z External Id SAMEA2340760 INSDC center name Instituto Gulbenkian de Ciencia INSDC first public 2014-10-02T17:04:02Z INSDC last update 2018-03-08T17:09:20Z INSDC status public Submitter Id E-MTAB-2270:NS5_Ascl1 broker name ArrayExpress cell line NS5 cell type neuronal stem cell common name house mouse genotype pBABE-Ascl1-ERT2-IRES-GFP-puro sample name E-MTAB-2270:NS5_Ascl1 scientific_name Mus musculus