ERS18116822 SAMEA115182457 2053615 Spirochaetia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 2be5ec0b-e531-44d3-8862-87bab0114b8e assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 85.2 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.53 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 45 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 2be5ec0b-e531-44d3-8862-87bab0114b8e scientific_name Spirochaetia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Spirochaetia_2 ERS18116857 SAMEA115182492 2358460 Nitrospirales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 0e4000e4-4fa3-4ae7-86ba-393fbf4ea046 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.67 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.73 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 46 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 0e4000e4-4fa3-4ae7-86ba-393fbf4ea046 scientific_name Nitrospirales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Nitrospirales_1 ERS18116866 SAMEA115182501 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id c0228bcb-c4b8-462a-94e7-08031748cab8 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 92.36 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.14 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 52 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name c0228bcb-c4b8-462a-94e7-08031748cab8 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_3 ERS18116809 SAMEA115182444 2053589 Candidatus Paceibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 7fcec887-0df7-4db1-bf4b-9ebd879713d1 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 74.43 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.72 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 42 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 7fcec887-0df7-4db1-bf4b-9ebd879713d1 scientific_name Candidatus Paceibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Paceibacterota_4 ERS18116872 SAMEA115182507 2053615 Spirochaetia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 978a6f5d-7542-411a-95a3-dd801879e222 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.53 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 55 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 978a6f5d-7542-411a-95a3-dd801879e222 scientific_name Spirochaetia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Spirochaetia_1 ERS18116864 SAMEA115182499 1920242 Aliikangiella sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 4812a46b-8195-46f5-a293-128f7a7e884f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.27 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.45 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 4812a46b-8195-46f5-a293-128f7a7e884f scientific_name Aliikangiella sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Aliikangiella_sp_1 ERS18116805 SAMEA115182440 2358460 Nitrospirales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 33a636fe-5a2f-4d4b-9c9b-2bdaf2a5e283 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 63.48 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.91 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 45 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 33a636fe-5a2f-4d4b-9c9b-2bdaf2a5e283 scientific_name Nitrospirales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ANI tolid odCinKuek1.Nitrospirales_2 ERS18116807 SAMEA115182442 2053589 Candidatus Paceibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 38907e3d-c89f-4070-91b6-9d6c7d1cae9c assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 75.84 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 38 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 38907e3d-c89f-4070-91b6-9d6c7d1cae9c scientific_name Candidatus Paceibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Paceibacterota_2 ERS18116845 SAMEA115182480 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 32956cc0-fab5-43d0-b310-d76fe79994bd assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.99 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 51 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 32956cc0-fab5-43d0-b310-d76fe79994bd scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Dehalococcoidia_4 ERS18116859 SAMEA115182494 2026781 Candidatus Poribacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id d0822ab9-b4e2-4c08-ac22-a2a69ee7900f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.58 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.12 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name d0822ab9-b4e2-4c08-ac22-a2a69ee7900f scientific_name Candidatus Poribacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Poribacteria_2 ERS18116863 SAMEA115182498 1913988 Alphaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 09f1bc1b-1bad-4b92-9679-6e0ab018260f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 98.26 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.99 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 53 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 09f1bc1b-1bad-4b92-9679-6e0ab018260f scientific_name Alphaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Alphaproteobacteria_5 ERS18116795 SAMEA115182430 2306054 Rhodothermales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 2fa9fb8b-a02e-48e4-8b48-3c09b0172eb4 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 83.28 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.13 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 2fa9fb8b-a02e-48e4-8b48-3c09b0172eb4 scientific_name Rhodothermales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Rhodothermales_1 ERS18116833 SAMEA115182468 2910147 Vicinamibacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 33854e28-373d-43bf-a5c3-6fc065bbcf3f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 95.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.58 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 71 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 33854e28-373d-43bf-a5c3-6fc065bbcf3f scientific_name Vicinamibacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Vicinamibacterales_2 ERS18116835 SAMEA115182470 2910147 Vicinamibacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1d3aa380-3d16-43da-b993-60d5eb5b4b02 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.44 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.85 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 63 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1d3aa380-3d16-43da-b993-60d5eb5b4b02 scientific_name Vicinamibacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Vicinamibacterales_4 ERS18116873 SAMEA115182508 312019 uncultured Candidatus Entotheonella sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id cfaa4de9-a9c6-4b59-82e3-79f4103bd15e assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 91.6 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.56 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name cfaa4de9-a9c6-4b59-82e3-79f4103bd15e scientific_name uncultured Candidatus Entotheonella sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Entotheonella_sp_1 ERS18116865 SAMEA115182500 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 16632d52-ba02-4dd1-a452-c1d69d7653ac assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 86.64 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.1 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 53 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 16632d52-ba02-4dd1-a452-c1d69d7653ac scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_2 ERS18116861 SAMEA115182496 1913988 Alphaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 5964c9ee-5f0d-4f7f-9443-08f8201d4ec3 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.5 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 43 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 5964c9ee-5f0d-4f7f-9443-08f8201d4ec3 scientific_name Alphaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Alphaproteobacteria_2 ERS18116791 SAMEA115182426 2026803 Candidatus Woesearchaeota archaeon 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 14fb1772-5ce7-4c26-9d7e-670ee10c0ddf assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 59.29 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 7.79 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 19 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 14fb1772-5ce7-4c26-9d7e-670ee10c0ddf scientific_name Candidatus Woesearchaeota archaeon sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Woesearchaeota_archaeon_1 ERS18116841 SAMEA115182476 2614250 Caldilineaceae bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3f5651a3-4c64-4ddd-a15f-43440b2599a7 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 92.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 3f5651a3-4c64-4ddd-a15f-43440b2599a7 scientific_name Caldilineaceae bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Caldilineaceae_1 ERS18116826 SAMEA115182461 2024843 Nitrosopumilus sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1ae46461-07d9-4201-ae73-64b79862995f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 99.03 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.97 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 36 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1ae46461-07d9-4201-ae73-64b79862995f scientific_name Nitrosopumilus sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Nitrosopumilus_sp_2 ERS18116838 SAMEA115182473 2687297 Oligoflexales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1cafa77f-b81c-4af4-95b6-bd9291313caa assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 85.85 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.79 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 35 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1cafa77f-b81c-4af4-95b6-bd9291313caa scientific_name Oligoflexales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Oligoflexales_1 ERS18116855 SAMEA115182490 2053570 Candidatus Latescibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id bd5b79a9-654a-4f06-b92f-bece6890e083 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.7 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.3 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 47 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name bd5b79a9-654a-4f06-b92f-bece6890e083 scientific_name Candidatus Latescibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Latescibacteria_1 ERS18116825 SAMEA115182460 2024843 Nitrosopumilus sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 6da61c57-4e1e-4728-84cc-dbd21aacc638 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 99.03 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.97 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 47 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 6da61c57-4e1e-4728-84cc-dbd21aacc638 scientific_name Nitrosopumilus sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Nitrosopumilus_sp_1 ERS18116810 SAMEA115182445 1913988 Alphaproteobacteria bacterium 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 68a36835-a892-45f0-ad75-c755bedf3c53 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 59.46 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.87 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 33 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 68a36835-a892-45f0-ad75-c755bedf3c53 scientific_name Alphaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Alphaproteobacteria_3 ERS18116821 SAMEA115182456 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id e6336b6f-3ad3-41d1-8354-04402ce6af36 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 82.99 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 8.64 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name e6336b6f-3ad3-41d1-8354-04402ce6af36 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_9 ERS18116851 SAMEA115182486 3036252 uncultured Candidatus Binatia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id c3ca01ca-9b7f-48ee-8e12-6a9662520b33 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.28 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.52 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 47 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name c3ca01ca-9b7f-48ee-8e12-6a9662520b33 scientific_name uncultured Candidatus Binatia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Binatia_2 ERS18116801 SAMEA115182436 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 42b919f2-92cc-4dc3-b199-0876fbf8b2b0 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 79.33 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.99 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 50 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 42b919f2-92cc-4dc3-b199-0876fbf8b2b0 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Dehalococcoidia_8 ERS18116831 SAMEA115182466 2651171 Thermoanaerobaculia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 8c3e8a6b-c03f-4b87-9f33-7e3e6cbd280f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.87 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.71 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 53 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 8c3e8a6b-c03f-4b87-9f33-7e3e6cbd280f scientific_name Thermoanaerobaculia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Thermoanaerobaculia_1 ERS18116816 SAMEA115182451 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 63e3d196-8771-426d-b942-1e969762af4d assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 73.17 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.41 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 63e3d196-8771-426d-b942-1e969762af4d scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_7 ERS18116839 SAMEA115182474 34094 Simkania sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 4bf22539-4948-455f-9ad4-f3850cc35ef8 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.96 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.23 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 35 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 4bf22539-4948-455f-9ad4-f3850cc35ef8 scientific_name Simkania sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Simkania_sp_1 ERS18116811 SAMEA115182446 1970157 Kordiimonas sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 0c4fb211-ca64-49ee-bd89-e79f41b28779 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 78.23 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 6.46 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 43 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 0c4fb211-ca64-49ee-bd89-e79f41b28779 scientific_name Kordiimonas sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Kordiimonas_sp_1 ERS18116792 SAMEA115182427 2479206 Cenarchaeum sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 0358f3d6-462d-42ae-b08e-2ebfc3ffa426 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.69 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.46 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 0358f3d6-462d-42ae-b08e-2ebfc3ffa426 scientific_name Cenarchaeum sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Cenarchaeum_sp_1 ERS18116812 SAMEA115182447 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 91805024-1808-4c63-a4d5-cb2a9de8bb45 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 84.0 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 42 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 91805024-1808-4c63-a4d5-cb2a9de8bb45 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_1 ERS18116830 SAMEA115182465 2081523 Terriglobia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id c15e5db1-7159-4592-9896-25da90492a0a assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.01 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.07 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name c15e5db1-7159-4592-9896-25da90492a0a scientific_name Terriglobia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Terriglobia_1 ERS18116797 SAMEA115182432 2053517 Bdellovibrionales bacterium 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 37a8a06c-99ff-48a6-a0b0-6e701cbade61 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 84.52 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.89 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 33 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 37a8a06c-99ff-48a6-a0b0-6e701cbade61 scientific_name Bdellovibrionales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Bdellovibrionales_2 ERS18116836 SAMEA115182471 2201156 Acidimicrobiales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3d3f7782-a936-4ff1-83ac-e603352f3468 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.58 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.28 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 53 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 3d3f7782-a936-4ff1-83ac-e603352f3468 scientific_name Acidimicrobiales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Acidimicrobiales_1 ERS18116828 SAMEA115182463 2026791 Bryobacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id ed11b7c5-ea46-40d8-b3f2-1352c6ddd74d assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.15 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.42 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 52 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name ed11b7c5-ea46-40d8-b3f2-1352c6ddd74d scientific_name Bryobacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Bryobacterales_2 ERS18116843 SAMEA115182478 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 80dece80-02d0-4e71-89cf-4e35314ec78f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.7 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.99 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 80dece80-02d0-4e71-89cf-4e35314ec78f scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Dehalococcoidia_2 ERS18116820 SAMEA115182455 1891229 Pseudomonadales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 504c100c-d5fc-4c5f-b718-e6b689804781 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 85.58 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.34 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 45 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 504c100c-d5fc-4c5f-b718-e6b689804781 scientific_name Pseudomonadales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ANI tolid odCinKuek1.Pseudomonadales_3 ERS18116824 SAMEA115182459 2202732 Nitrosopumilaceae archaeon 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 389b3062-a251-4925-bfa2-0b5b4056803b assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.57 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.94 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 55 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 389b3062-a251-4925-bfa2-0b5b4056803b scientific_name Nitrosopumilaceae archaeon sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification fully defined by topology tolid odCinKuek1.Nitrosopumilaceae_archaeon_1 ERS18116803 SAMEA115182438 2774603 Candidatus Krumholzibacteriia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id ddce77f4-5b02-4236-82b8-f190a5ab5e62 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 87.72 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.1 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 47 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name ddce77f4-5b02-4236-82b8-f190a5ab5e62 scientific_name Candidatus Krumholzibacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Krumholzibacteria_1 ERS18116868 SAMEA115182503 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id de6e77df-a423-4627-98da-beae8f250cee assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 83.53 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 42 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name de6e77df-a423-4627-98da-beae8f250cee scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_8 ERS18116842 SAMEA115182477 2614250 Caldilineaceae bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1a963e88-e386-4f35-86cd-9ea7058ee1bf assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.36 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 50 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1a963e88-e386-4f35-86cd-9ea7058ee1bf scientific_name Caldilineaceae bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Caldilineaceae_2 ERS18116827 SAMEA115182462 2026791 Bryobacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id d9fe4927-0271-4d34-8a35-7101854e6c69 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.44 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.56 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 51 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name d9fe4927-0271-4d34-8a35-7101854e6c69 scientific_name Bryobacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Bryobacterales_1 ERS18116796 SAMEA115182431 2053517 Bdellovibrionales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id e0da37f2-157a-41be-a4e0-f88196eb6e23 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 91.07 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 7.59 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 34 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name e0da37f2-157a-41be-a4e0-f88196eb6e23 scientific_name Bdellovibrionales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Bdellovibrionales_1 ERS18116813 SAMEA115182448 2847306 Candidatus Thiodiazotropha sp. 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id e8cad701-fdb6-49d2-9016-45504c741e1a assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 71.07 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.28 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 27 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name e8cad701-fdb6-49d2-9016-45504c741e1a scientific_name Candidatus Thiodiazotropha sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ani_screen tolid odCinKuek1.Thiodiazotropha_sp_1 ERS18116874 SAMEA115182509 1869227 bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 9be6d20c-2acc-4803-ae17-f4ce1a23f777 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 90.84 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.02 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 246 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 9be6d20c-2acc-4803-ae17-f4ce1a23f777 scientific_name bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Bacteria_1 ERS18116848 SAMEA115182483 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 8ae34c4e-956f-4af6-a887-bf289f41e209 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.7 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 54 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 8ae34c4e-956f-4af6-a887-bf289f41e209 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Dehalococcoidia_7 ERS18116823 SAMEA115182458 2212473 Kiritimatiellia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id a58bb831-2b45-48c7-b9e4-f81b1e0a8b30 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 85.56 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.94 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name a58bb831-2b45-48c7-b9e4-f81b1e0a8b30 scientific_name Kiritimatiellia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Kiritimatiellia_1 ERS18116840 SAMEA115182475 2952883 Aggregatilineales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 615d4995-bd2f-4452-af07-51a2ff7b996e assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 91.82 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 615d4995-bd2f-4452-af07-51a2ff7b996e scientific_name Aggregatilineales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ANI tolid odCinKuek1.Aggregatilineales_1 ERS18116818 SAMEA115182453 3039487 uncultured candidatus Porisulfidus sp. 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1ae7e275-4e84-42ab-bdc5-42fc156bcf43 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 61.27 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.06 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 28 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1ae7e275-4e84-42ab-bdc5-42fc156bcf43 scientific_name uncultured candidatus Porisulfidus sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Porisulfidus_sp_2 ERS18116858 SAMEA115182493 2026781 Candidatus Poribacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 82c2f847-b3a6-4516-8f18-366283cf6473 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.99 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.7 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 82c2f847-b3a6-4516-8f18-366283cf6473 scientific_name Candidatus Poribacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Poribacteria_1 ERS18116834 SAMEA115182469 2910147 Vicinamibacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 8ba7f8d7-3fc5-437a-86da-c0f89745c60d assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 95.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.05 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 61 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 8ba7f8d7-3fc5-437a-86da-c0f89745c60d scientific_name Vicinamibacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Vicinamibacterales_3 ERS18116844 SAMEA115182479 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 7f999ff8-d283-48c4-a118-127608cdb325 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.73 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.99 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 50 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 7f999ff8-d283-48c4-a118-127608cdb325 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Dehalococcoidia_3 ERS18116869 SAMEA115182504 1891229 Pseudomonadales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 00d0c908-70e7-49bf-9df5-6506a71f4a6f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 85.26 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.42 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 00d0c908-70e7-49bf-9df5-6506a71f4a6f scientific_name Pseudomonadales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Pseudomonadales_1 ERS18116856 SAMEA115182491 2053570 Candidatus Latescibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id a5674b02-4162-4bfe-8498-4cf7be1abab7 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.99 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.6 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 46 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name a5674b02-4162-4bfe-8498-4cf7be1abab7 scientific_name Candidatus Latescibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Latescibacteria_2 ERS18116847 SAMEA115182482 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 6b45779a-ebf8-4e4c-9ead-68fbda15a8e5 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.7 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 51 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 6b45779a-ebf8-4e4c-9ead-68fbda15a8e5 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Dehalococcoidia_6 ERS18116806 SAMEA115182441 2053589 Candidatus Paceibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 7e0b2b80-9296-4bcc-b9c9-d74b82f53a9f assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 77.72 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.72 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 38 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 7e0b2b80-9296-4bcc-b9c9-d74b82f53a9f scientific_name Candidatus Paceibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Paceibacterota_1 ERS18116815 SAMEA115182450 1913989 Gammaproteobacteria bacterium 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 89c3df47-2259-45eb-8c08-3ea56f678644 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 47.85 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.14 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 31 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 89c3df47-2259-45eb-8c08-3ea56f678644 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_5 ERS18116837 SAMEA115182472 2201156 Acidimicrobiales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3c0e4d9b-b625-46d2-b0a6-8909d0d11ce2 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.16 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.28 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 51 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 3c0e4d9b-b625-46d2-b0a6-8909d0d11ce2 scientific_name Acidimicrobiales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Acidimicrobiales_2 ERS18116829 SAMEA115182464 2026791 Bryobacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 74d63353-912c-4bc9-bbc2-a65f7fa8b3d4 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 91.49 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.56 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 56 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 74d63353-912c-4bc9-bbc2-a65f7fa8b3d4 scientific_name Bryobacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Bryobacterales_3 ERS18116849 SAMEA115182484 2026724 Chloroflexota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id c2a6a117-7fdc-414d-9db2-dee508dba2e6 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 92.28 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.21 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 52 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name c2a6a117-7fdc-414d-9db2-dee508dba2e6 scientific_name Chloroflexota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Chloroflexota_1 ERS18116804 SAMEA115182439 2053570 Candidatus Latescibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 605a1296-4225-481d-be83-785710d5d95b assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 49.39 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.4 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 18 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 605a1296-4225-481d-be83-785710d5d95b scientific_name Candidatus Latescibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Latescibacteria_3 ERS18116808 SAMEA115182443 2053589 Candidatus Paceibacterota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 93a98f80-d0a8-401f-8808-a66c26cb9ca1 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 72.39 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 43 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 93a98f80-d0a8-401f-8808-a66c26cb9ca1 scientific_name Candidatus Paceibacterota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Paceibacterota_3 ERS18116854 SAMEA115182489 2910168 Longimicrobiales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id f1d7cd51-80ea-44bf-852b-8dd3c8245deb assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.8 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 4.4 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name f1d7cd51-80ea-44bf-852b-8dd3c8245deb scientific_name Longimicrobiales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Longimicrobiales_1 ERS18116846 SAMEA115182481 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 7f9773e7-94d2-4cdc-9af9-5f80bc638774 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.22 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 7f9773e7-94d2-4cdc-9af9-5f80bc638774 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Dehalococcoidia_5 ERS18116793 SAMEA115182428 2651171 Thermoanaerobaculia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id be1ad86c-a805-415c-a519-1364f37dff8a assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.02 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.71 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 54 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name be1ad86c-a805-415c-a519-1364f37dff8a scientific_name Thermoanaerobaculia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Thermoanaerobaculia_2 ERS18116850 SAMEA115182485 3036252 uncultured Candidatus Binatia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 1ea5a42d-c5b0-4d9d-a207-f079449ea76f assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.77 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.36 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 1ea5a42d-c5b0-4d9d-a207-f079449ea76f scientific_name uncultured Candidatus Binatia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Binatia_1 ERS18116862 SAMEA115182497 1913988 Alphaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 2e7a10f6-f36c-43d1-a3df-2601a6acf5df assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.01 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.26 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 50 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 2e7a10f6-f36c-43d1-a3df-2601a6acf5df scientific_name Alphaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Alphaproteobacteria_4 ERS18116814 SAMEA115182449 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 34d517c9-cb9b-4d47-a341-13b43577154e assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 62.53 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 2.76 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 34d517c9-cb9b-4d47-a341-13b43577154e scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_4 ERS18116870 SAMEA115182505 114707 uncultured Pseudomonas sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 85a798c0-0cd6-4dda-9c23-19d369d3a037 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 99.9 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.54 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 62 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 85a798c0-0cd6-4dda-9c23-19d369d3a037 scientific_name uncultured Pseudomonas sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ani_screen tolid odCinKuek1.Pseudomonas_sp_1 ERS18116867 SAMEA115182502 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id e3892392-5858-422b-b2e0-55b344cbf6f5 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 92.34 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.86 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 41 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name e3892392-5858-422b-b2e0-55b344cbf6f5 scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Gammaproteobacteria_6 ERS18116800 SAMEA115182435 2026734 Dehalococcoidia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 195ffa29-62a4-4469-8e29-02ad0f975ef2 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 92.41 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 9.9 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 56 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 195ffa29-62a4-4469-8e29-02ad0f975ef2 scientific_name Dehalococcoidia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Dehalococcoidia_1 ERS18116871 SAMEA115182506 1913989 Gammaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id ff55764a-aadf-4709-b722-887e21b4041b assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 89.54 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.05 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 39 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name ff55764a-aadf-4709-b722-887e21b4041b scientific_name Gammaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Gammaproteobacteria_10 ERS18116832 SAMEA115182467 2910147 Vicinamibacterales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 30d25617-1a2a-4ddc-b9f5-a158cac5d056 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 98.29 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.63 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 57 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 30d25617-1a2a-4ddc-b9f5-a158cac5d056 scientific_name Vicinamibacterales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification fully defined by topology tolid odCinKuek1.Vicinamibacterales_1 ERS18116875 SAMEA115182510 1191521 uncultured Pedosphaera sp. 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3c3bcd29-09cb-4248-805a-399234c8352e assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 97.97 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.68 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 49 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 3c3bcd29-09cb-4248-805a-399234c8352e scientific_name uncultured Pedosphaera sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Pedosphaera_sp_1 ERS18116819 SAMEA115182454 1891229 Pseudomonadales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 3f92b29c-ec59-40e2-bde4-f40a5ff3c98d assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 86.14 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.42 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 3f92b29c-ec59-40e2-bde4-f40a5ff3c98d scientific_name Pseudomonadales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Pseudomonadales_2 ERS18116853 SAMEA115182488 2202153 Thermodesulfobacteriota bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 562d5969-4c6f-403f-ab6b-3350694af712 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 96.64 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 3.36 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 562d5969-4c6f-403f-ab6b-3350694af712 scientific_name Thermodesulfobacteriota bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Thermodesulfobacteriota_1 ERS18116852 SAMEA115182487 3036252 uncultured Candidatus Binatia bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 31174faa-af5d-46df-8d7f-4a8f69c76524 assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 94.17 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.94 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 50 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 31174faa-af5d-46df-8d7f-4a8f69c76524 scientific_name uncultured Candidatus Binatia bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Binatia_3 ERS18116817 SAMEA115182452 3039487 uncultured candidatus Porisulfidus sp. 16S recovered No 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 8f1e5305-75fe-4f23-b45d-e97029704257 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 71.48 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.82 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 40 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 8f1e5305-75fe-4f23-b45d-e97029704257 scientific_name uncultured candidatus Porisulfidus sp. sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Porisulfidus_sp_1 ERS18116860 SAMEA115182495 1913988 Alphaproteobacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 704cdb66-9897-4ec8-b8cd-f14a69034c9a assembly quality Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 93.78 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.01 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 52 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 704cdb66-9897-4ec8-b8cd-f14a69034c9a scientific_name Alphaproteobacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Alphaproteobacteria_1 ERS18116790 SAMEA115182425 2910166 Candidatus Nanoarchaeia archaeon 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id b1a1bc76-af8c-4573-bb13-809aee09e5f1 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 55.53 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 30 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name b1a1bc76-af8c-4573-bb13-809aee09e5f1 scientific_name Candidatus Nanoarchaeia archaeon sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification fully defined by topology tolid odCinKuek1.Nanoarchaeia_archaeon_1 ERS18116799 SAMEA115182434 2952883 Aggregatilineales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 820cf046-b612-4aa0-8071-c81a8552c568 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 90.0 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.0 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 44 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 820cf046-b612-4aa0-8071-c81a8552c568 scientific_name Aggregatilineales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ANI tolid odCinKuek1.Aggregatilineales_2 ERS18116794 SAMEA115182429 2793154 Vicinamibacteria bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id f52ce835-e115-4c9f-a9bf-b5c4ffc57e7f assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 65.5 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 8.72 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 48 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name f52ce835-e115-4c9f-a9bf-b5c4ffc57e7f scientific_name Vicinamibacteria bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker ANI tolid odCinKuek1.Vicinamibacteria_1 ERS18116802 SAMEA115182437 1665022 uncultured Xenococcaceae cyanobacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 015e979e-655d-484a-8046-9dc17aa5478d assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 95.26 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 0.3 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 70 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 015e979e-655d-484a-8046-9dc17aa5478d scientific_name uncultured Xenococcaceae cyanobacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic novelty determined using RED tolid odCinKuek1.Xenococcaceae_cyanobacterium_1 ERS18116798 SAMEA115182433 2053517 Bdellovibrionales bacterium 16S recovered Yes 16S recovery software PROKKA (version 1.14.5) ENA first public 2024-02-07 GAL Geomar Helmholtz Centre GAL_sample_id NOT PROVIDED INSDC center name Tree of Life Programme INSDC status public MAG coverage software Minimap2 (version 2.24-r1122) Submitter Id 0a0c76aa-3ed4-42af-b660-007c45599fe2 assembly quality Many fragments with little to no review of assembly other than reporting of standard assembly statistics assembly software metaMDBG binning parameters coverage; graph; hic-mapping binning software MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR broad-scale environmental context aquatic biome collected_by JOE LOPEZ collecting institution NOVA SOUTHEASTERN UNIVERSITY collection date 2020-12-16 completeness score 86.16 completeness software checkM (version 1.2.1); checkM_DB (release 2015-01-16) contamination score 1.12 depth 7.6 environmental medium adult sponge tissue geographic location (country and/or sea) USA geographic location (latitude) 26.15 geographic location (longitude) -80.10 geographic location (region and locality) Florida|Broward County|Offshore Fort Lauderdale habitat Limestone ledge host life stage adult host scientific name Cinachyrella kuekenthali host sex not collected host taxid 458489 identified_by JOE LOPEZ identifier_affiliation NOVA SOUTHEASTERN UNIVERSITY investigation type metagenome-assembled genome isolation_source Sponge: Cinachyrella kuekenthali local environmental context marine benthic biome metagenomic source sponge metagenome number of standard tRNAs extracted 35 organism part WHOLE ORGANISM project name ASG sample derived from SAMEA115182424 sample name 0a0c76aa-3ed4-42af-b660-007c45599fe2 scientific_name Bdellovibrionales bacterium sequencing method Pacbio Sequel II specimen_id GHC0000167 specimen_voucher Y, in ethanol tRNA extraction software PROKKA (version 1.14.5) taxonomic classification GTDB-TK (version 2.3.2); GTDB (release 214) taxonomic identity marker taxonomic classification defined by topology and ANI tolid odCinKuek1.Bdellovibrionales_3