ERX402086 E-MTAB-2321:assay_2 E-MTAB-2321:assay_2 ERP004884 E-MTAB-2321 RNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicing ERS403449 SAMEA2358423 E-MTAB-2321:fandango_rep2 fandango_rep2 RNA-Seq TRANSCRIPTOMIC RANDOM Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNase I (Promega) treatment during 30 min at 37C. After, the DNase was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs) 100 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2000 Experimental Factor: genotype fand1 ERX402087 E-MTAB-2321:assay_4 E-MTAB-2321:assay_4 ERP004884 E-MTAB-2321 RNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicing ERS403450 SAMEA2358424 E-MTAB-2321:wildtype_rep2 wildtype_rep2 RNA-Seq TRANSCRIPTOMIC RANDOM Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNase I (Promega) treatment during 30 min at 37C. After, the DNase was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs) 100 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype ERX402088 E-MTAB-2321:assay_3 E-MTAB-2321:assay_3 ERP004884 E-MTAB-2321 RNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicing ERS403451 SAMEA2358425 E-MTAB-2321:wildtype_rep1 wildtype_rep1 RNA-Seq TRANSCRIPTOMIC RANDOM Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNase I (Promega) treatment during 30 min at 37C. After, the DNase was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs) 100 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2000 Experimental Factor: genotype wild type genotype ERX402089 E-MTAB-2321:assay_1 E-MTAB-2321:assay_1 ERP004884 E-MTAB-2321 RNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicing ERS403452 SAMEA2358426 E-MTAB-2321:fandango_rep1 fandango_rep1 RNA-Seq TRANSCRIPTOMIC RANDOM Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008). Flies were raised using standard techniques. Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNase I (Promega) treatment during 30 min at 37C. After, the DNase was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol. cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs) 100 0 F Application Read Forward 1 1 R Application Read Reverse 101 Illumina HiSeq 2000 Experimental Factor: genotype fand1