ERS435079 SAMEA2465314 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:16:58Z External Id SAMEA2465314 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:16:58Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-Total(2) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-Total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435080 SAMEA2465315 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:17:12Z External Id SAMEA2465315 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:17:12Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-Total(1) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-Total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435082 SAMEA2465317 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:16:57Z External Id SAMEA2465317 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:16:57Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-IP(3) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435083 SAMEA2465318 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:17:11Z External Id SAMEA2465318 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:17:11Z INSDC status public Submitter Id E-MTAB-2464:EAP1-Total(3) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-Total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435084 SAMEA2465319 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:36Z External Id SAMEA2465319 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:36Z INSDC status public Submitter Id E-MTAB-2464:EAP1-IP(3) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435085 SAMEA2465320 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:36Z External Id SAMEA2465320 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:36Z INSDC status public Submitter Id E-MTAB-2464:Pab1-IP(2) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435086 SAMEA2465321 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:36Z External Id SAMEA2465321 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:36Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-Total(3) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-Total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435087 SAMEA2465322 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:36Z External Id SAMEA2465322 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:36Z INSDC status public Submitter Id E-MTAB-2464:EAP1-Total(2) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-Total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435088 SAMEA2465323 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465323 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:EAP1-IP(2) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435089 SAMEA2465324 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465324 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:Pab1-IP(3) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435090 SAMEA2465325 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465325 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-Total(2) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-Total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435091 SAMEA2465326 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465326 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-Total(1) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-Total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435092 SAMEA2465327 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465327 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-IP(1) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435093 SAMEA2465328 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465328 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-IP(3) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435094 SAMEA2465329 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465329 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-IP(1) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435095 SAMEA2465330 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:20:45Z External Id SAMEA2465330 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:20:45Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-IP(2) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435096 SAMEA2465331 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:00Z External Id SAMEA2465331 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:00Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-Total(2) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-Total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435097 SAMEA2465332 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:00Z External Id SAMEA2465332 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:00Z INSDC status public Submitter Id E-MTAB-2464:CAF20-IP(3) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435098 SAMEA2465333 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:00Z External Id SAMEA2465333 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:00Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-IP(1) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435099 SAMEA2465334 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:01Z External Id SAMEA2465334 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:01Z INSDC status public Submitter Id E-MTAB-2464:CAF20-Total(3) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-Total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435100 SAMEA2465335 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:01Z External Id SAMEA2465335 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:01Z INSDC status public Submitter Id E-MTAB-2464:Pab1-IP(1) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435101 SAMEA2465336 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:01Z External Id SAMEA2465336 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:01Z INSDC status public Submitter Id E-MTAB-2464:CAF20-IP(2) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435102 SAMEA2465337 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:01Z External Id SAMEA2465337 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:01Z INSDC status public Submitter Id E-MTAB-2464:CAF20-Total(1) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-Total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435103 SAMEA2465338 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:01Z External Id SAMEA2465338 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:01Z INSDC status public Submitter Id E-MTAB-2464:CAF20-IP(1) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435104 SAMEA2465339 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465339 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:Pab1-total(3) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435081 SAMEA2465316 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:16:58Z External Id SAMEA2465316 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:16:58Z INSDC status public Submitter Id E-MTAB-2464:Pab1-total(1) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435106 SAMEA2465341 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465341 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-IP(2) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435107 SAMEA2465342 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465342 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:EAP1-IP(1) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-IP(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435108 SAMEA2465343 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465343 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:EAP1-Total(1) broker name ArrayExpress common name baker's yeast genotype Eap1-tap-tag sample name E-MTAB-2464:EAP1-Total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435109 SAMEA2465344 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465344 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-Total(1) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-Total(1) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435110 SAMEA2465345 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465345 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:CAF20-Total(2) broker name ArrayExpress common name baker's yeast genotype Caf20-tap-tag sample name E-MTAB-2464:CAF20-Total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435111 SAMEA2465346 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465346 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:eIF4G2-IP(3) broker name ArrayExpress common name baker's yeast genotype eIF4G2-tap-tag sample name E-MTAB-2464:eIF4G2-IP(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435112 SAMEA2465347 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:27Z External Id SAMEA2465347 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:27Z INSDC status public Submitter Id E-MTAB-2464:Pab1-total(2) broker name ArrayExpress common name baker's yeast genotype Pab1-tap-tag sample name E-MTAB-2464:Pab1-total(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435113 SAMEA2465348 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:27Z External Id SAMEA2465348 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:27Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-IP(2) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-IP(2) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435114 SAMEA2465349 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:27Z External Id SAMEA2465349 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:27Z INSDC status public Submitter Id E-MTAB-2464:eIF4G1-Total(3) broker name ArrayExpress common name baker's yeast genotype eIF4G1-tap-tag sample name E-MTAB-2464:eIF4G1-Total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS435105 SAMEA2465340 4932 Saccharomyces cerevisiae Protocols: Grown at 30C overnight to an OD600 0.6, pelleted by centrifugation and snap frozen into liquid nitrogen Affinity chromatography is used to pulldown the protein of interest with attached mRNAs. The protein of interest has a protein A tag encoded on the genome of the yeast strain and IgG (the same antibody is used across all samples) magnetic beads are used to pull out this protein from whole cell extracts. The RNA is processed from these samples and compared to total RNA samples from the same strains. samples ground under liquid nitrogen in lysis buffer using a 6870 Freezer Mill (Spex). Lysate thawed on ice, clarified by centrifugation and nucleic acids extract using Trizol Reagent. rRNA was depleted from samples using Ribominus Eukaryote Kit, ethanol precipitated and resuspended in DEPC water. Sequencing libraries were then generated using the whole Transcriptome Library Preparation protocol provided with the SOLiD Total RNA-Seq Kit. . cDNA was then amplified and barcoded with SOLiD, RNA barcoding Kit and samples purified using PureLink, PCR Micro Kit ENA-FIRST-PUBLIC 2014-08-01T17:01:08Z ENA-LAST-UPDATE 2018-03-08T17:21:14Z External Id SAMEA2465340 INSDC center name The University of Manchester INSDC first public 2014-08-01T17:01:08Z INSDC last update 2018-03-08T17:21:14Z INSDC status public Submitter Id E-MTAB-2464:eIF4E-Total(3) broker name ArrayExpress common name baker's yeast genotype eIF4E-tap-tag sample name E-MTAB-2464:eIF4E-Total(3) scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741