ERS445010 SAMEA2475632 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475632 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:WT_ovaries_2 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification wild type genotype hs-Flp / +; FRTG13 / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:WT_ovaries_2 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445011 SAMEA2475633 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475633 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[2]_ovaries_1 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[2] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[2]_ovaries_1 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445012 SAMEA2475634 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475634 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:WT_ovaries_1 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification wild type genotype hs-Flp / +; FRTG13 / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:WT_ovaries_1 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445013 SAMEA2475635 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475635 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[1]_ovaries_1 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[1] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[1]_ovaries_1 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445014 SAMEA2475636 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475636 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[2]_ovaries_3 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[2] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[2]_ovaries_3 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445015 SAMEA2475637 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475637 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:WT_ovaries_3 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification wild type genotype hs-Flp / +; FRTG13 / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:WT_ovaries_3 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445016 SAMEA2475638 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475638 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[1]_ovaries_3 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[1] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[1]_ovaries_3 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445017 SAMEA2475639 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475639 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[1]_ovaries_2 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[1] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[1]_ovaries_2 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+ ERS445018 SAMEA2475640 7227 Drosophila melanogaster Protocols: Drosophila melanogaster was bred at Stowers Institute. The enok1 and enok2 mutant alleles originated from Bloomington Stock Center. Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching. Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters. ENA-FIRST-PUBLIC 2014-10-22T17:01:00Z ENA-LAST-UPDATE 2018-03-08T17:28:46Z External Id SAMEA2475640 INSDC center name Stowers Institute for Medical Research INSDC first public 2014-10-22T17:01:00Z INSDC last update 2018-03-08T17:28:46Z INSDC status public Submitter Id E-MTAB-2521:enok[2]_ovaries_2 age 4-6 day broker name ArrayExpress common name fruit fly developmental stage adult genetic modification EMS induced mutation genotype hs-Flp / +; FRTG13, enok[2] / FRTG13, ovo[D1-18] organism part stages 5-14 egg chambers sample name E-MTAB-2521:enok[2]_ovaries_2 scientific_name Drosophila melanogaster sex female specimen with known storage state fresh specimen strain yw/+