ERX520175 qiime_experiment_2401:1269760:444 qiime_experiment_2401:1269760:444 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501682 SAMEA2635328 qiime_study_2401:Plate7.90 Plate7.90:444 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102808 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 444 target_gene 16S rRNA study_center Cornell ERX520176 qiime_experiment_2401:1269762:472 qiime_experiment_2401:1269762:472 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501684 SAMEA2635330 qiime_study_2401:Plate8.36 Plate8.36:472 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68704 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 472 target_gene 16S rRNA study_center Cornell ERX520177 qiime_experiment_2401:1269763:334 qiime_experiment_2401:1269763:334 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501685 SAMEA2635331 qiime_study_2401:Plate6.54 Plate6.54:334 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74154 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 334 target_gene 16S rRNA study_center Cornell ERX520178 qiime_experiment_2401:1269764:493 qiime_experiment_2401:1269764:493 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501686 SAMEA2635332 qiime_study_2401:Plate8.67 Plate8.67:493 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 55976 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 493 target_gene 16S rRNA study_center Cornell ERX520269 qiime_experiment_2401:1269861:381 qiime_experiment_2401:1269861:381 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501783 SAMEA2635429 qiime_study_2401:Plate7.17 Plate7.17:381 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76544 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 381 target_gene 16S rRNA study_center Cornell ERX520270 qiime_experiment_2401:1269306:394 qiime_experiment_2401:1269306:394 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501784 SAMEA2635430 qiime_study_2401:Plate7.30 Plate7.30:394 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83537 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 394 target_gene 16S rRNA study_center Cornell ERX520271 qiime_experiment_2401:1269307:217 qiime_experiment_2401:1269307:217 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501785 SAMEA2635431 qiime_study_2401:Plate5.18 Plate5.18:217 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69737 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 217 target_gene 16S rRNA study_center Cornell ERX520272 qiime_experiment_2401:1269308:206 qiime_experiment_2401:1269308:206 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501786 SAMEA2635432 qiime_study_2401:Plate16.74 Plate16.74:206 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 206 target_gene 16S rRNA study_center Cornell ERX520273 qiime_experiment_2401:1269310:22 qiime_experiment_2401:1269310:22 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501788 SAMEA2635434 qiime_study_2401:Plate14.40 Plate14.40:22 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60045 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 22 target_gene 16S rRNA study_center Cornell ERX520274 qiime_experiment_2401:1269311:62 qiime_experiment_2401:1269311:62 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501789 SAMEA2635435 qiime_study_2401:Plate14.90 Plate14.90:62 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69283 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 62 target_gene 16S rRNA study_center Cornell ERX520451 qiime_experiment_2401:1269496:331 qiime_experiment_2401:1269496:331 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501974 SAMEA2635620 qiime_study_2401:Plate6.51 Plate6.51:331 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65788 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 331 target_gene 16S rRNA study_center Cornell ERX520452 qiime_experiment_2401:1269497:498 qiime_experiment_2401:1269497:498 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501975 SAMEA2635621 qiime_study_2401:Plate8.83 Plate8.83:498 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74508 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 498 target_gene 16S rRNA study_center Cornell ERX520453 qiime_experiment_2401:1269498:282 qiime_experiment_2401:1269498:282 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501976 SAMEA2635622 qiime_study_2401:Plate5.87 Plate5.87:282 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84105 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 282 target_gene 16S rRNA study_center Cornell ERX520454 qiime_experiment_2401:1269499:43 qiime_experiment_2401:1269499:43 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501977 SAMEA2635623 qiime_study_2401:Plate14.65 Plate14.65:43 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 97813 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 43 target_gene 16S rRNA study_center Cornell ERX520455 qiime_experiment_2401:1269500:165 qiime_experiment_2401:1269500:165 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501978 SAMEA2635624 qiime_study_2401:Plate16.34 Plate16.34:165 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 165 target_gene 16S rRNA study_center Cornell ERX520456 qiime_experiment_2401:1269501:430 qiime_experiment_2401:1269501:430 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501979 SAMEA2635625 qiime_study_2401:Plate7.75 Plate7.75:430 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88918 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 430 target_gene 16S rRNA study_center Cornell ERX520547 qiime_experiment_2401:1269595:474 qiime_experiment_2401:1269595:474 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502073 SAMEA2635719 qiime_study_2401:Plate8.4 Plate8.4:474 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 108049 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 474 target_gene 16S rRNA study_center Cornell ERX520548 qiime_experiment_2401:1269596:319 qiime_experiment_2401:1269596:319 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502074 SAMEA2635720 qiime_study_2401:Plate6.39 Plate6.39:319 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 61117 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 319 target_gene 16S rRNA study_center Cornell ERX520549 qiime_experiment_2401:1269597:535 qiime_experiment_2401:1269597:535 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502075 SAMEA2635721 qiime_study_2401:Plate9.6 Plate9.6:535 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 44261 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 535 target_gene 16S rRNA study_center Cornell ERX520550 qiime_experiment_2401:1269598:506 qiime_experiment_2401:1269598:506 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502076 SAMEA2635722 qiime_study_2401:Plate9.20 Plate9.20:506 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89545 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 506 target_gene 16S rRNA study_center Cornell ERX520551 qiime_experiment_2401:1269599:372 qiime_experiment_2401:1269599:372 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502077 SAMEA2635723 qiime_study_2401:Plate6.92 Plate6.92:372 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77993 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 372 target_gene 16S rRNA study_center Cornell ERX520552 qiime_experiment_2401:1269600:500 qiime_experiment_2401:1269600:500 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502078 SAMEA2635724 qiime_study_2401:Plate8.88 Plate8.88:500 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 50975 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 500 target_gene 16S rRNA study_center Cornell ERX520643 qiime_experiment_2401:1269701:354 qiime_experiment_2401:1269701:354 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502179 SAMEA2635825 qiime_study_2401:Plate6.74 Plate6.74:354 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88934 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 354 target_gene 16S rRNA study_center Cornell ERX520644 qiime_experiment_2401:1269702:209 qiime_experiment_2401:1269702:209 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502180 SAMEA2635826 qiime_study_2401:Plate5.1 Plate5.1:209 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75958 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 209 target_gene 16S rRNA study_center Cornell ERX520645 qiime_experiment_2401:1269703:235 qiime_experiment_2401:1269703:235 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502181 SAMEA2635827 qiime_study_2401:Plate5.38 Plate5.38:235 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71068 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 235 target_gene 16S rRNA study_center Cornell ERX520646 qiime_experiment_2401:1269704:325 qiime_experiment_2401:1269704:325 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502182 SAMEA2635828 qiime_study_2401:Plate6.44 Plate6.44:325 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70402 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 325 target_gene 16S rRNA study_center Cornell ERX520647 qiime_experiment_2401:1269705:221 qiime_experiment_2401:1269705:221 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502183 SAMEA2635829 qiime_study_2401:Plate5.23 Plate5.23:221 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80843 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 221 target_gene 16S rRNA study_center Cornell ERX520648 qiime_experiment_2401:1269706:246 qiime_experiment_2401:1269706:246 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502184 SAMEA2635830 qiime_study_2401:Plate5.50 Plate5.50:246 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82888 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 246 target_gene 16S rRNA study_center Cornell ERX520179 qiime_experiment_2401:1269765:295 qiime_experiment_2401:1269765:295 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501687 SAMEA2635333 qiime_study_2401:Plate6.14 Plate6.14:295 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65436 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 295 target_gene 16S rRNA study_center Cornell ERX520180 qiime_experiment_2401:1269766:236 qiime_experiment_2401:1269766:236 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501688 SAMEA2635334 qiime_study_2401:Plate5.39 Plate5.39:236 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74885 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 236 target_gene 16S rRNA study_center Cornell ERX520181 qiime_experiment_2401:1269767:172 qiime_experiment_2401:1269767:172 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501689 SAMEA2635335 qiime_study_2401:Plate16.42 Plate16.42:172 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 172 target_gene 16S rRNA study_center Cornell ERX520182 qiime_experiment_2401:1269768:482 qiime_experiment_2401:1269768:482 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501690 SAMEA2635336 qiime_study_2401:Plate8.48 Plate8.48:482 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76400 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 482 target_gene 16S rRNA study_center Cornell ERX520183 qiime_experiment_2401:1269769:373 qiime_experiment_2401:1269769:373 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501691 SAMEA2635337 qiime_study_2401:Plate6.93 Plate6.93:373 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74579 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 373 target_gene 16S rRNA study_center Cornell ERX520184 qiime_experiment_2401:1269770:348 qiime_experiment_2401:1269770:348 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501692 SAMEA2635338 qiime_study_2401:Plate6.69 Plate6.69:348 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77199 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 348 target_gene 16S rRNA study_center Cornell ERX520275 qiime_experiment_2401:1269312:107 qiime_experiment_2401:1269312:107 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501790 SAMEA2635436 qiime_study_2401:Plate15.54 Plate15.54:107 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 53669 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 107 target_gene 16S rRNA study_center Cornell ERX520276 qiime_experiment_2401:1269313:125 qiime_experiment_2401:1269313:125 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501791 SAMEA2635437 qiime_study_2401:Plate15.79 Plate15.79:125 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 40235 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 125 target_gene 16S rRNA study_center Cornell ERX520277 qiime_experiment_2401:1269314:413 qiime_experiment_2401:1269314:413 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501792 SAMEA2635438 qiime_study_2401:Plate7.57 Plate7.57:413 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90397 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 413 target_gene 16S rRNA study_center Cornell ERX520278 qiime_experiment_2401:1269315:408 qiime_experiment_2401:1269315:408 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501793 SAMEA2635439 qiime_study_2401:Plate7.51 Plate7.51:408 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93878 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 408 target_gene 16S rRNA study_center Cornell ERX520279 qiime_experiment_2401:1269316:438 qiime_experiment_2401:1269316:438 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501794 SAMEA2635440 qiime_study_2401:Plate7.83 Plate7.83:438 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74367 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 438 target_gene 16S rRNA study_center Cornell ERX520280 qiime_experiment_2401:1269317:253 qiime_experiment_2401:1269317:253 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501795 SAMEA2635441 qiime_study_2401:Plate5.57 Plate5.57:253 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75113 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 253 target_gene 16S rRNA study_center Cornell ERX520365 qiime_experiment_2401:1269408:425 qiime_experiment_2401:1269408:425 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501886 SAMEA2635532 qiime_study_2401:Plate7.70 Plate7.70:425 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92090 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 425 target_gene 16S rRNA study_center Cornell ERX520366 qiime_experiment_2401:1269409:553 qiime_experiment_2401:1269409:553 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501887 SAMEA2635533 qiime_study_2401:Plate9.9 Plate9.9:553 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 104421 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 553 target_gene 16S rRNA study_center Cornell ERX520457 qiime_experiment_2401:1269502:242 qiime_experiment_2401:1269502:242 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501980 SAMEA2635626 qiime_study_2401:Plate5.45 Plate5.45:242 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85750 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 242 target_gene 16S rRNA study_center Cornell ERX520458 qiime_experiment_2401:1269503:417 qiime_experiment_2401:1269503:417 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501981 SAMEA2635627 qiime_study_2401:Plate7.61 Plate7.61:417 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75829 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 417 target_gene 16S rRNA study_center Cornell ERX520459 qiime_experiment_2401:1269504:313 qiime_experiment_2401:1269504:313 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501982 SAMEA2635628 qiime_study_2401:Plate6.31 Plate6.31:313 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71365 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 313 target_gene 16S rRNA study_center Cornell ERX520460 qiime_experiment_2401:1269505:192 qiime_experiment_2401:1269505:192 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501983 SAMEA2635629 qiime_study_2401:Plate16.61 Plate16.61:192 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 192 target_gene 16S rRNA study_center Cornell ERX520461 qiime_experiment_2401:1269506:302 qiime_experiment_2401:1269506:302 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501984 SAMEA2635630 qiime_study_2401:Plate6.20 Plate6.20:302 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79782 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 302 target_gene 16S rRNA study_center Cornell ERX520462 qiime_experiment_2401:1269507:38 qiime_experiment_2401:1269507:38 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501985 SAMEA2635631 qiime_study_2401:Plate14.60 Plate14.60:38 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78788 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 38 target_gene 16S rRNA study_center Cornell ERX520553 qiime_experiment_2401:1269601:85 qiime_experiment_2401:1269601:85 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502079 SAMEA2635725 qiime_study_2401:Plate15.27 Plate15.27:85 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64733 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 85 target_gene 16S rRNA study_center Cornell ERX520554 qiime_experiment_2401:1269602:7 qiime_experiment_2401:1269602:7 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502080 SAMEA2635726 qiime_study_2401:Plate14.2 Plate14.2:7 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74718 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 7 target_gene 16S rRNA study_center Cornell ERX520555 qiime_experiment_2401:1269604:224 qiime_experiment_2401:1269604:224 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502082 SAMEA2635728 qiime_study_2401:Plate5.27 Plate5.27:224 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67649 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 224 target_gene 16S rRNA study_center Cornell ERX520556 qiime_experiment_2401:1269605:26 qiime_experiment_2401:1269605:26 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502083 SAMEA2635729 qiime_study_2401:Plate14.46 Plate14.46:26 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64678 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 26 target_gene 16S rRNA study_center Cornell ERX520557 qiime_experiment_2401:1269606:497 qiime_experiment_2401:1269606:497 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502084 SAMEA2635730 qiime_study_2401:Plate8.8 Plate8.8:497 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 112316 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 497 target_gene 16S rRNA study_center Cornell ERX520558 qiime_experiment_2401:1269607:383 qiime_experiment_2401:1269607:383 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502085 SAMEA2635731 qiime_study_2401:Plate7.19 Plate7.19:383 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87567 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 383 target_gene 16S rRNA study_center Cornell ERX520649 qiime_experiment_2401:1269707:365 qiime_experiment_2401:1269707:365 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502185 SAMEA2635831 qiime_study_2401:Plate6.84 Plate6.84:365 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87870 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 365 target_gene 16S rRNA study_center Cornell ERX520650 qiime_experiment_2401:1269709:358 qiime_experiment_2401:1269709:358 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502187 SAMEA2635833 qiime_study_2401:Plate6.78 Plate6.78:358 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59402 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 358 target_gene 16S rRNA study_center Cornell ERX520651 qiime_experiment_2401:1269710:57 qiime_experiment_2401:1269710:57 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502188 SAMEA2635834 qiime_study_2401:Plate14.81 Plate14.81:57 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93306 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 57 target_gene 16S rRNA study_center Cornell ERX520652 qiime_experiment_2401:1269711:323 qiime_experiment_2401:1269711:323 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502189 SAMEA2635835 qiime_study_2401:Plate6.42 Plate6.42:323 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75496 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 323 target_gene 16S rRNA study_center Cornell ERX520653 qiime_experiment_2401:1269712:511 qiime_experiment_2401:1269712:511 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502190 SAMEA2635836 qiime_study_2401:Plate9.27 Plate9.27:511 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81643 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 511 target_gene 16S rRNA study_center Cornell ERX520654 qiime_experiment_2401:1269713:271 qiime_experiment_2401:1269713:271 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502191 SAMEA2635837 qiime_study_2401:Plate5.76 Plate5.76:271 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81248 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 271 target_gene 16S rRNA study_center Cornell ERX520185 qiime_experiment_2401:1269771:439 qiime_experiment_2401:1269771:439 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501693 SAMEA2635339 qiime_study_2401:Plate7.84 Plate7.84:439 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 105823 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 439 target_gene 16S rRNA study_center Cornell ERX520186 qiime_experiment_2401:1269772:393 qiime_experiment_2401:1269772:393 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501694 SAMEA2635340 qiime_study_2401:Plate7.3 Plate7.3:393 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 114651 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 393 target_gene 16S rRNA study_center Cornell ERX520187 qiime_experiment_2401:1269773:357 qiime_experiment_2401:1269773:357 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501695 SAMEA2635341 qiime_study_2401:Plate6.77 Plate6.77:357 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71614 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 357 target_gene 16S rRNA study_center Cornell ERX520188 qiime_experiment_2401:1269774:494 qiime_experiment_2401:1269774:494 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501696 SAMEA2635342 qiime_study_2401:Plate8.7 Plate8.7:494 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 124297 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 494 target_gene 16S rRNA study_center Cornell ERX520189 qiime_experiment_2401:1269775:138 qiime_experiment_2401:1269775:138 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501697 SAMEA2635343 qiime_study_2401:Plate15.95 Plate15.95:138 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 38849 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 138 target_gene 16S rRNA study_center Cornell ERX520190 qiime_experiment_2401:1269776:179 qiime_experiment_2401:1269776:179 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501698 SAMEA2635344 qiime_study_2401:Plate16.5 Plate16.5:179 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 179 target_gene 16S rRNA study_center Cornell ERX520281 qiime_experiment_2401:1269318:96 qiime_experiment_2401:1269318:96 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501796 SAMEA2635442 qiime_study_2401:Plate15.40 Plate15.40:96 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81918 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 96 target_gene 16S rRNA study_center Cornell ERX520282 qiime_experiment_2401:1269319:258 qiime_experiment_2401:1269319:258 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501797 SAMEA2635443 qiime_study_2401:Plate5.63 Plate5.63:258 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82649 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 258 target_gene 16S rRNA study_center Cornell ERX520283 qiime_experiment_2401:1269320:260 qiime_experiment_2401:1269320:260 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501798 SAMEA2635444 qiime_study_2401:Plate5.65 Plate5.65:260 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79868 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 260 target_gene 16S rRNA study_center Cornell ERX520284 qiime_experiment_2401:1269321:168 qiime_experiment_2401:1269321:168 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501799 SAMEA2635445 qiime_study_2401:Plate16.38 Plate16.38:168 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 168 target_gene 16S rRNA study_center Cornell ERX520285 qiime_experiment_2401:1269322:450 qiime_experiment_2401:1269322:450 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501800 SAMEA2635446 qiime_study_2401:Plate8.11 Plate8.11:450 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90518 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 450 target_gene 16S rRNA study_center Cornell ERX520286 qiime_experiment_2401:1269323:467 qiime_experiment_2401:1269323:467 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501801 SAMEA2635447 qiime_study_2401:Plate8.31 Plate8.31:467 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 110545 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 467 target_gene 16S rRNA study_center Cornell ERX520367 qiime_experiment_2401:1269410:281 qiime_experiment_2401:1269410:281 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501888 SAMEA2635534 qiime_study_2401:Plate5.86 Plate5.86:281 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84409 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 281 target_gene 16S rRNA study_center Cornell ERX520368 qiime_experiment_2401:1269411:11 qiime_experiment_2401:1269411:11 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501889 SAMEA2635535 qiime_study_2401:Plate14.26 Plate14.26:11 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88712 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 11 target_gene 16S rRNA study_center Cornell ERX520369 qiime_experiment_2401:1269412:256 qiime_experiment_2401:1269412:256 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501890 SAMEA2635536 qiime_study_2401:Plate5.61 Plate5.61:256 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 42267 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 256 target_gene 16S rRNA study_center Cornell ERX520370 qiime_experiment_2401:1269413:509 qiime_experiment_2401:1269413:509 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501891 SAMEA2635537 qiime_study_2401:Plate9.25 Plate9.25:509 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 110699 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 509 target_gene 16S rRNA study_center Cornell ERX520371 qiime_experiment_2401:1269414:315 qiime_experiment_2401:1269414:315 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501892 SAMEA2635538 qiime_study_2401:Plate6.33 Plate6.33:315 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80218 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 315 target_gene 16S rRNA study_center Cornell ERX520372 qiime_experiment_2401:1269415:233 qiime_experiment_2401:1269415:233 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501893 SAMEA2635539 qiime_study_2401:Plate5.35 Plate5.35:233 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71589 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 233 target_gene 16S rRNA study_center Cornell ERX520463 qiime_experiment_2401:1269508:12 qiime_experiment_2401:1269508:12 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501986 SAMEA2635632 qiime_study_2401:Plate14.27 Plate14.27:12 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72438 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 12 target_gene 16S rRNA study_center Cornell ERX520464 qiime_experiment_2401:1269509:294 qiime_experiment_2401:1269509:294 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501987 SAMEA2635633 qiime_study_2401:Plate6.13 Plate6.13:294 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66541 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 294 target_gene 16S rRNA study_center Cornell ERX520465 qiime_experiment_2401:1269510:406 qiime_experiment_2401:1269510:406 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501988 SAMEA2635634 qiime_study_2401:Plate7.5 Plate7.5:406 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 104062 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 406 target_gene 16S rRNA study_center Cornell ERX520466 qiime_experiment_2401:1269511:279 qiime_experiment_2401:1269511:279 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501989 SAMEA2635635 qiime_study_2401:Plate5.83 Plate5.83:279 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81676 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 279 target_gene 16S rRNA study_center Cornell ERX520467 qiime_experiment_2401:1269512:135 qiime_experiment_2401:1269512:135 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501990 SAMEA2635636 qiime_study_2401:Plate15.91 Plate15.91:135 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60968 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 135 target_gene 16S rRNA study_center Cornell ERX520468 qiime_experiment_2401:1269513:16 qiime_experiment_2401:1269513:16 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501991 SAMEA2635637 qiime_study_2401:Plate14.33 Plate14.33:16 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74808 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 16 target_gene 16S rRNA study_center Cornell ERX520559 qiime_experiment_2401:1269608:533 qiime_experiment_2401:1269608:533 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502086 SAMEA2635732 qiime_study_2401:Plate9.58 Plate9.58:533 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82566 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 533 target_gene 16S rRNA study_center Cornell ERX520560 qiime_experiment_2401:1269609:351 qiime_experiment_2401:1269609:351 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502087 SAMEA2635733 qiime_study_2401:Plate6.71 Plate6.71:351 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92136 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 351 target_gene 16S rRNA study_center Cornell ERX520659 qiime_experiment_2401:1269718:31 qiime_experiment_2401:1269718:31 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502196 SAMEA2635842 qiime_study_2401:Plate14.53 Plate14.53:31 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69146 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 31 target_gene 16S rRNA study_center Cornell ERX520660 qiime_experiment_2401:1269719:363 qiime_experiment_2401:1269719:363 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502197 SAMEA2635843 qiime_study_2401:Plate6.82 Plate6.82:363 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65023 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 363 target_gene 16S rRNA study_center Cornell ERX520191 qiime_experiment_2401:1269778:13 qiime_experiment_2401:1269778:13 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501700 SAMEA2635346 qiime_study_2401:Plate14.28 Plate14.28:13 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 95709 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 13 target_gene 16S rRNA study_center Cornell ERX520192 qiime_experiment_2401:1269779:245 qiime_experiment_2401:1269779:245 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501701 SAMEA2635347 qiime_study_2401:Plate5.49 Plate5.49:245 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 114337 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 245 target_gene 16S rRNA study_center Cornell ERX520193 qiime_experiment_2401:1269780:501 qiime_experiment_2401:1269780:501 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501702 SAMEA2635348 qiime_study_2401:Plate8.89 Plate8.89:501 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 42777 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 501 target_gene 16S rRNA study_center Cornell ERX520194 qiime_experiment_2401:1269781:84 qiime_experiment_2401:1269781:84 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501703 SAMEA2635349 qiime_study_2401:Plate15.26 Plate15.26:84 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 57327 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 84 target_gene 16S rRNA study_center Cornell ERX520195 qiime_experiment_2401:1269782:129 qiime_experiment_2401:1269782:129 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501704 SAMEA2635350 qiime_study_2401:Plate15.83 Plate15.83:129 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 56709 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 129 target_gene 16S rRNA study_center Cornell ERX520196 qiime_experiment_2401:1269783:117 qiime_experiment_2401:1269783:117 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501705 SAMEA2635351 qiime_study_2401:Plate15.66 Plate15.66:117 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58217 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 117 target_gene 16S rRNA study_center Cornell ERX520287 qiime_experiment_2401:1269324:332 qiime_experiment_2401:1269324:332 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501802 SAMEA2635448 qiime_study_2401:Plate6.52 Plate6.52:332 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77149 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 332 target_gene 16S rRNA study_center Cornell ERX520288 qiime_experiment_2401:1269325:69 qiime_experiment_2401:1269325:69 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501803 SAMEA2635449 qiime_study_2401:Plate15.10 Plate15.10:69 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 51593 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 69 target_gene 16S rRNA study_center Cornell ERX520289 qiime_experiment_2401:1269326:309 qiime_experiment_2401:1269326:309 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501804 SAMEA2635450 qiime_study_2401:Plate6.28 Plate6.28:309 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76061 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 309 target_gene 16S rRNA study_center Cornell ERX520290 qiime_experiment_2401:1269327:453 qiime_experiment_2401:1269327:453 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501805 SAMEA2635451 qiime_study_2401:Plate8.16 Plate8.16:453 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 38581 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 453 target_gene 16S rRNA study_center Cornell ERX520291 qiime_experiment_2401:1269328:324 qiime_experiment_2401:1269328:324 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501806 SAMEA2635452 qiime_study_2401:Plate6.43 Plate6.43:324 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 61624 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 324 target_gene 16S rRNA study_center Cornell ERX520292 qiime_experiment_2401:1269329:525 qiime_experiment_2401:1269329:525 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501807 SAMEA2635453 qiime_study_2401:Plate9.46 Plate9.46:525 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72042 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 525 target_gene 16S rRNA study_center Cornell ERX520373 qiime_experiment_2401:1269416:539 qiime_experiment_2401:1269416:539 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501894 SAMEA2635540 qiime_study_2401:Plate9.64 Plate9.64:539 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76183 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 539 target_gene 16S rRNA study_center Cornell ERX520374 qiime_experiment_2401:1269417:412 qiime_experiment_2401:1269417:412 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501895 SAMEA2635541 qiime_study_2401:Plate7.55 Plate7.55:412 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80146 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 412 target_gene 16S rRNA study_center Cornell ERX520375 qiime_experiment_2401:1269418:518 qiime_experiment_2401:1269418:518 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501896 SAMEA2635542 qiime_study_2401:Plate9.35 Plate9.35:518 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60638 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 518 target_gene 16S rRNA study_center Cornell ERX520376 qiime_experiment_2401:1269419:540 qiime_experiment_2401:1269419:540 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501897 SAMEA2635543 qiime_study_2401:Plate9.65 Plate9.65:540 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82740 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 540 target_gene 16S rRNA study_center Cornell ERX520377 qiime_experiment_2401:1269420:3 qiime_experiment_2401:1269420:3 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501898 SAMEA2635544 qiime_study_2401:Plate14.16 Plate14.16:3 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 101698 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 3 target_gene 16S rRNA study_center Cornell ERX520378 qiime_experiment_2401:1269421:311 qiime_experiment_2401:1269421:311 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501899 SAMEA2635545 qiime_study_2401:Plate6.3 Plate6.3:311 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 115956 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 311 target_gene 16S rRNA study_center Cornell ERX520469 qiime_experiment_2401:1269514:4 qiime_experiment_2401:1269514:4 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501992 SAMEA2635638 qiime_study_2401:Plate14.17 Plate14.17:4 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59069 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 4 target_gene 16S rRNA study_center Cornell ERX520470 qiime_experiment_2401:1269515:465 qiime_experiment_2401:1269515:465 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501993 SAMEA2635639 qiime_study_2401:Plate8.29 Plate8.29:465 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 113902 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 465 target_gene 16S rRNA study_center Cornell ERX520471 qiime_experiment_2401:1269516:72 qiime_experiment_2401:1269516:72 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501994 SAMEA2635640 qiime_study_2401:Plate15.13 Plate15.13:72 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 55277 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 72 target_gene 16S rRNA study_center Cornell ERX520472 qiime_experiment_2401:1269517:389 qiime_experiment_2401:1269517:389 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501995 SAMEA2635641 qiime_study_2401:Plate7.25 Plate7.25:389 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 96664 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 389 target_gene 16S rRNA study_center Cornell ERX520473 qiime_experiment_2401:1269518:459 qiime_experiment_2401:1269518:459 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501996 SAMEA2635642 qiime_study_2401:Plate8.21 Plate8.21:459 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77367 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 459 target_gene 16S rRNA study_center Cornell ERX520474 qiime_experiment_2401:1269519:382 qiime_experiment_2401:1269519:382 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501997 SAMEA2635643 qiime_study_2401:Plate7.18 Plate7.18:382 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70992 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 382 target_gene 16S rRNA study_center Cornell ERX520565 qiime_experiment_2401:1269614:243 qiime_experiment_2401:1269614:243 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502092 SAMEA2635738 qiime_study_2401:Plate5.47 Plate5.47:243 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78937 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 243 target_gene 16S rRNA study_center Cornell ERX520566 qiime_experiment_2401:1269615:416 qiime_experiment_2401:1269615:416 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502093 SAMEA2635739 qiime_study_2401:Plate7.60 Plate7.60:416 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82910 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 416 target_gene 16S rRNA study_center Cornell ERX520567 qiime_experiment_2401:1269616:542 qiime_experiment_2401:1269616:542 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502094 SAMEA2635740 qiime_study_2401:Plate9.68 Plate9.68:542 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78460 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 542 target_gene 16S rRNA study_center Cornell ERX520568 qiime_experiment_2401:1269617:25 qiime_experiment_2401:1269617:25 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502095 SAMEA2635741 qiime_study_2401:Plate14.44 Plate14.44:25 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64344 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 25 target_gene 16S rRNA study_center Cornell ERX520569 qiime_experiment_2401:1269618:78 qiime_experiment_2401:1269618:78 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502096 SAMEA2635742 qiime_study_2401:Plate15.19 Plate15.19:78 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62514 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 78 target_gene 16S rRNA study_center Cornell ERX520570 qiime_experiment_2401:1269619:360 qiime_experiment_2401:1269619:360 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502097 SAMEA2635743 qiime_study_2401:Plate6.8 Plate6.8:360 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87266 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 360 target_gene 16S rRNA study_center Cornell ERX520661 qiime_experiment_2401:1269720:545 qiime_experiment_2401:1269720:545 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502198 SAMEA2635844 qiime_study_2401:Plate9.72 Plate9.72:545 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86497 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 545 target_gene 16S rRNA study_center Cornell ERX520662 qiime_experiment_2401:1269721:296 qiime_experiment_2401:1269721:296 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502199 SAMEA2635845 qiime_study_2401:Plate6.15 Plate6.15:296 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85901 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 296 target_gene 16S rRNA study_center Cornell ERX520663 qiime_experiment_2401:1269723:33 qiime_experiment_2401:1269723:33 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502201 SAMEA2635847 qiime_study_2401:Plate14.55 Plate14.55:33 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94096 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 33 target_gene 16S rRNA study_center Cornell ERX520664 qiime_experiment_2401:1269724:536 qiime_experiment_2401:1269724:536 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502202 SAMEA2635848 qiime_study_2401:Plate9.60 Plate9.60:536 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68461 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 536 target_gene 16S rRNA study_center Cornell ERX520665 qiime_experiment_2401:1269726:288 qiime_experiment_2401:1269726:288 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502204 SAMEA2635850 qiime_study_2401:Plate5.92 Plate5.92:288 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81616 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 288 target_gene 16S rRNA study_center Cornell ERX520666 qiime_experiment_2401:1269727:94 qiime_experiment_2401:1269727:94 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502205 SAMEA2635851 qiime_study_2401:Plate15.38 Plate15.38:94 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64568 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 94 target_gene 16S rRNA study_center Cornell ERX520197 qiime_experiment_2401:1269784:28 qiime_experiment_2401:1269784:28 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501706 SAMEA2635352 qiime_study_2401:Plate14.49 Plate14.49:28 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102391 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 28 target_gene 16S rRNA study_center Cornell ERX520198 qiime_experiment_2401:1269785:395 qiime_experiment_2401:1269785:395 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501707 SAMEA2635353 qiime_study_2401:Plate7.31 Plate7.31:395 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77664 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 395 target_gene 16S rRNA study_center Cornell ERX520199 qiime_experiment_2401:1269786:109 qiime_experiment_2401:1269786:109 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501708 SAMEA2635354 qiime_study_2401:Plate15.56 Plate15.56:109 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86634 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 109 target_gene 16S rRNA study_center Cornell ERX520200 qiime_experiment_2401:1269788:207 qiime_experiment_2401:1269788:207 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501710 SAMEA2635356 qiime_study_2401:Plate16.8 Plate16.8:207 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 192671 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 207 target_gene 16S rRNA study_center Cornell ERX520201 qiime_experiment_2401:1269789:93 qiime_experiment_2401:1269789:93 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501711 SAMEA2635357 qiime_study_2401:Plate15.37 Plate15.37:93 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79880 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 93 target_gene 16S rRNA study_center Cornell ERX520202 qiime_experiment_2401:1269790:456 qiime_experiment_2401:1269790:456 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501712 SAMEA2635358 qiime_study_2401:Plate8.19 Plate8.19:456 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 128229 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 456 target_gene 16S rRNA study_center Cornell ERX520293 qiime_experiment_2401:1269330:276 qiime_experiment_2401:1269330:276 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501808 SAMEA2635454 qiime_study_2401:Plate5.80 Plate5.80:276 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79335 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 276 target_gene 16S rRNA study_center Cornell ERX520294 qiime_experiment_2401:1269331:336 qiime_experiment_2401:1269331:336 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501809 SAMEA2635455 qiime_study_2401:Plate6.56 Plate6.56:336 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60702 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 336 target_gene 16S rRNA study_center Cornell ERX520295 qiime_experiment_2401:1269332:547 qiime_experiment_2401:1269332:547 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501810 SAMEA2635456 qiime_study_2401:Plate9.75 Plate9.75:547 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 99325 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 547 target_gene 16S rRNA study_center Cornell ERX520296 qiime_experiment_2401:1269333:141 qiime_experiment_2401:1269333:141 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501811 SAMEA2635457 qiime_study_2401:Plate16.10 Plate16.10:141 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 19 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 141 target_gene 16S rRNA study_center Cornell ERX520297 qiime_experiment_2401:1269334:268 qiime_experiment_2401:1269334:268 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501812 SAMEA2635458 qiime_study_2401:Plate5.72 Plate5.72:268 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79951 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 268 target_gene 16S rRNA study_center Cornell ERX520298 qiime_experiment_2401:1269335:249 qiime_experiment_2401:1269335:249 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501813 SAMEA2635459 qiime_study_2401:Plate5.53 Plate5.53:249 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68203 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 249 target_gene 16S rRNA study_center Cornell ERX520379 qiime_experiment_2401:1269423:398 qiime_experiment_2401:1269423:398 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501901 SAMEA2635547 qiime_study_2401:Plate7.35 Plate7.35:398 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65587 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 398 target_gene 16S rRNA study_center Cornell ERX520380 qiime_experiment_2401:1269424:254 qiime_experiment_2401:1269424:254 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501902 SAMEA2635548 qiime_study_2401:Plate5.58 Plate5.58:254 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69074 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 254 target_gene 16S rRNA study_center Cornell ERX520381 qiime_experiment_2401:1269425:283 qiime_experiment_2401:1269425:283 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501903 SAMEA2635549 qiime_study_2401:Plate5.88 Plate5.88:283 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69541 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 283 target_gene 16S rRNA study_center Cornell ERX520382 qiime_experiment_2401:1269426:339 qiime_experiment_2401:1269426:339 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501904 SAMEA2635550 qiime_study_2401:Plate6.6 Plate6.6:339 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82311 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 339 target_gene 16S rRNA study_center Cornell ERX520383 qiime_experiment_2401:1269427:469 qiime_experiment_2401:1269427:469 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501905 SAMEA2635551 qiime_study_2401:Plate8.33 Plate8.33:469 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81626 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 469 target_gene 16S rRNA study_center Cornell ERX520384 qiime_experiment_2401:1269428:376 qiime_experiment_2401:1269428:376 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501906 SAMEA2635552 qiime_study_2401:Plate7.11 Plate7.11:376 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102153 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 376 target_gene 16S rRNA study_center Cornell ERX520475 qiime_experiment_2401:1269520:52 qiime_experiment_2401:1269520:52 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501998 SAMEA2635644 qiime_study_2401:Plate14.76 Plate14.76:52 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70516 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 52 target_gene 16S rRNA study_center Cornell ERX520476 qiime_experiment_2401:1269521:214 qiime_experiment_2401:1269521:214 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501999 SAMEA2635645 qiime_study_2401:Plate5.15 Plate5.15:214 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75017 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 214 target_gene 16S rRNA study_center Cornell ERX520477 qiime_experiment_2401:1269522:0 qiime_experiment_2401:1269522:0 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502000 SAMEA2635646 qiime_study_2401:Plate14.13 Plate14.13:0 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58257 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 0 target_gene 16S rRNA study_center Cornell ERX520478 qiime_experiment_2401:1269523:448 qiime_experiment_2401:1269523:448 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502001 SAMEA2635647 qiime_study_2401:Plate8.1 Plate8.1:448 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65863 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 448 target_gene 16S rRNA study_center Cornell ERX520479 qiime_experiment_2401:1269524:27 qiime_experiment_2401:1269524:27 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502002 SAMEA2635648 qiime_study_2401:Plate14.48 Plate14.48:27 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69394 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 27 target_gene 16S rRNA study_center Cornell ERX520480 qiime_experiment_2401:1269525:40 qiime_experiment_2401:1269525:40 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502003 SAMEA2635649 qiime_study_2401:Plate14.62 Plate14.62:40 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72839 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 40 target_gene 16S rRNA study_center Cornell ERX520571 qiime_experiment_2401:1269620:478 qiime_experiment_2401:1269620:478 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502098 SAMEA2635744 qiime_study_2401:Plate8.43 Plate8.43:478 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 43119 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 478 target_gene 16S rRNA study_center Cornell ERX520572 qiime_experiment_2401:1269623:359 qiime_experiment_2401:1269623:359 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502101 SAMEA2635747 qiime_study_2401:Plate6.79 Plate6.79:359 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84088 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 359 target_gene 16S rRNA study_center Cornell ERX520573 qiime_experiment_2401:1269624:167 qiime_experiment_2401:1269624:167 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502102 SAMEA2635748 qiime_study_2401:Plate16.37 Plate16.37:167 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 167 target_gene 16S rRNA study_center Cornell ERX520574 qiime_experiment_2401:1269625:20 qiime_experiment_2401:1269625:20 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502103 SAMEA2635749 qiime_study_2401:Plate14.39 Plate14.39:20 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82318 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 20 target_gene 16S rRNA study_center Cornell ERX520575 qiime_experiment_2401:1269626:410 qiime_experiment_2401:1269626:410 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502104 SAMEA2635750 qiime_study_2401:Plate7.53 Plate7.53:410 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68531 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 410 target_gene 16S rRNA study_center Cornell ERX520576 qiime_experiment_2401:1269627:520 qiime_experiment_2401:1269627:520 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502105 SAMEA2635751 qiime_study_2401:Plate9.38 Plate9.38:520 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66321 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 520 target_gene 16S rRNA study_center Cornell ERX520667 qiime_experiment_2401:1269728:153 qiime_experiment_2401:1269728:153 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502206 SAMEA2635852 qiime_study_2401:Plate16.23 Plate16.23:153 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 153 target_gene 16S rRNA study_center Cornell ERX520668 qiime_experiment_2401:1269729:74 qiime_experiment_2401:1269729:74 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502207 SAMEA2635853 qiime_study_2401:Plate15.15 Plate15.15:74 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74331 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 74 target_gene 16S rRNA study_center Cornell ERX520669 qiime_experiment_2401:1269730:436 qiime_experiment_2401:1269730:436 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502208 SAMEA2635854 qiime_study_2401:Plate7.80 Plate7.80:436 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69422 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 436 target_gene 16S rRNA study_center Cornell ERX520670 qiime_experiment_2401:1269731:114 qiime_experiment_2401:1269731:114 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502209 SAMEA2635855 qiime_study_2401:Plate15.61 Plate15.61:114 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64241 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 114 target_gene 16S rRNA study_center Cornell ERX520671 qiime_experiment_2401:1269732:229 qiime_experiment_2401:1269732:229 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502210 SAMEA2635856 qiime_study_2401:Plate5.31 Plate5.31:229 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71959 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 229 target_gene 16S rRNA study_center Cornell ERX520672 qiime_experiment_2401:1269733:63 qiime_experiment_2401:1269733:63 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502211 SAMEA2635857 qiime_study_2401:Plate14.92 Plate14.92:63 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73660 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 63 target_gene 16S rRNA study_center Cornell ERX520203 qiime_experiment_2401:1269791:516 qiime_experiment_2401:1269791:516 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501713 SAMEA2635359 qiime_study_2401:Plate9.33 Plate9.33:516 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 53011 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 516 target_gene 16S rRNA study_center Cornell ERX520204 qiime_experiment_2401:1269792:64 qiime_experiment_2401:1269792:64 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501714 SAMEA2635360 qiime_study_2401:Plate14.93 Plate14.93:64 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 49285 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 64 target_gene 16S rRNA study_center Cornell ERX520205 qiime_experiment_2401:1269793:265 qiime_experiment_2401:1269793:265 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501715 SAMEA2635361 qiime_study_2401:Plate5.7 Plate5.7:265 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83212 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 265 target_gene 16S rRNA study_center Cornell ERX520206 qiime_experiment_2401:1269794:1 qiime_experiment_2401:1269794:1 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501716 SAMEA2635362 qiime_study_2401:Plate14.14 Plate14.14:1 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 97296 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 1 target_gene 16S rRNA study_center Cornell ERX520207 qiime_experiment_2401:1269795:449 qiime_experiment_2401:1269795:449 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501717 SAMEA2635363 qiime_study_2401:Plate8.10 Plate8.10:449 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 145494 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 449 target_gene 16S rRNA study_center Cornell ERX520561 qiime_experiment_2401:1269610:495 qiime_experiment_2401:1269610:495 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502088 SAMEA2635734 qiime_study_2401:Plate8.72 Plate8.72:495 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 133570 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 495 target_gene 16S rRNA study_center Cornell ERX520562 qiime_experiment_2401:1269611:97 qiime_experiment_2401:1269611:97 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502089 SAMEA2635735 qiime_study_2401:Plate15.41 Plate15.41:97 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73115 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 97 target_gene 16S rRNA study_center Cornell ERX520563 qiime_experiment_2401:1269612:239 qiime_experiment_2401:1269612:239 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502090 SAMEA2635736 qiime_study_2401:Plate5.42 Plate5.42:239 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69086 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 239 target_gene 16S rRNA study_center Cornell ERX520564 qiime_experiment_2401:1269613:215 qiime_experiment_2401:1269613:215 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502091 SAMEA2635737 qiime_study_2401:Plate5.16 Plate5.16:215 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81663 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 215 target_gene 16S rRNA study_center Cornell ERX520655 qiime_experiment_2401:1269714:188 qiime_experiment_2401:1269714:188 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502192 SAMEA2635838 qiime_study_2401:Plate16.58 Plate16.58:188 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 132056 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 188 target_gene 16S rRNA study_center Cornell ERX520656 qiime_experiment_2401:1269715:521 qiime_experiment_2401:1269715:521 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502193 SAMEA2635839 qiime_study_2401:Plate9.40 Plate9.40:521 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 48855 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 521 target_gene 16S rRNA study_center Cornell ERX520657 qiime_experiment_2401:1269716:300 qiime_experiment_2401:1269716:300 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502194 SAMEA2635840 qiime_study_2401:Plate6.19 Plate6.19:300 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88670 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 300 target_gene 16S rRNA study_center Cornell ERX520658 qiime_experiment_2401:1269717:312 qiime_experiment_2401:1269717:312 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502195 SAMEA2635841 qiime_study_2401:Plate6.30 Plate6.30:312 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67520 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 312 target_gene 16S rRNA study_center Cornell ERX520208 qiime_experiment_2401:1269796:470 qiime_experiment_2401:1269796:470 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501718 SAMEA2635364 qiime_study_2401:Plate8.34 Plate8.34:470 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 120480 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 470 target_gene 16S rRNA study_center Cornell ERX520299 qiime_experiment_2401:1269337:104 qiime_experiment_2401:1269337:104 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501815 SAMEA2635461 qiime_study_2401:Plate15.51 Plate15.51:104 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 56180 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 104 target_gene 16S rRNA study_center Cornell ERX520300 qiime_experiment_2401:1269338:515 qiime_experiment_2401:1269338:515 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501816 SAMEA2635462 qiime_study_2401:Plate9.32 Plate9.32:515 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 98939 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 515 target_gene 16S rRNA study_center Cornell ERX520301 qiime_experiment_2401:1269339:123 qiime_experiment_2401:1269339:123 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501817 SAMEA2635463 qiime_study_2401:Plate15.75 Plate15.75:123 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 46198 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 123 target_gene 16S rRNA study_center Cornell ERX520302 qiime_experiment_2401:1269340:335 qiime_experiment_2401:1269340:335 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501818 SAMEA2635464 qiime_study_2401:Plate6.55 Plate6.55:335 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80465 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 335 target_gene 16S rRNA study_center Cornell ERX520303 qiime_experiment_2401:1269341:184 qiime_experiment_2401:1269341:184 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501819 SAMEA2635465 qiime_study_2401:Plate16.54 Plate16.54:184 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 184 target_gene 16S rRNA study_center Cornell ERX520304 qiime_experiment_2401:1269342:328 qiime_experiment_2401:1269342:328 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501820 SAMEA2635466 qiime_study_2401:Plate6.49 Plate6.49:328 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 11495 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 328 target_gene 16S rRNA study_center Cornell ERX520385 qiime_experiment_2401:1269429:385 qiime_experiment_2401:1269429:385 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501907 SAMEA2635553 qiime_study_2401:Plate7.20 Plate7.20:385 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75609 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 385 target_gene 16S rRNA study_center Cornell ERX520386 qiime_experiment_2401:1269430:195 qiime_experiment_2401:1269430:195 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501908 SAMEA2635554 qiime_study_2401:Plate16.64 Plate16.64:195 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 200462 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 195 target_gene 16S rRNA study_center Cornell ERX520387 qiime_experiment_2401:1269431:267 qiime_experiment_2401:1269431:267 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501909 SAMEA2635555 qiime_study_2401:Plate5.71 Plate5.71:267 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84255 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 267 target_gene 16S rRNA study_center Cornell ERX520388 qiime_experiment_2401:1269432:34 qiime_experiment_2401:1269432:34 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501910 SAMEA2635556 qiime_study_2401:Plate14.56 Plate14.56:34 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89344 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 34 target_gene 16S rRNA study_center Cornell ERX520389 qiime_experiment_2401:1269433:83 qiime_experiment_2401:1269433:83 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501911 SAMEA2635557 qiime_study_2401:Plate15.25 Plate15.25:83 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63727 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 83 target_gene 16S rRNA study_center Cornell ERX520390 qiime_experiment_2401:1269434:220 qiime_experiment_2401:1269434:220 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501912 SAMEA2635558 qiime_study_2401:Plate5.20 Plate5.20:220 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69870 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 220 target_gene 16S rRNA study_center Cornell ERX520481 qiime_experiment_2401:1269526:139 qiime_experiment_2401:1269526:139 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502004 SAMEA2635650 qiime_study_2401:Plate15.96 Plate15.96:139 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 52284 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 139 target_gene 16S rRNA study_center Cornell ERX520482 qiime_experiment_2401:1269527:345 qiime_experiment_2401:1269527:345 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502005 SAMEA2635651 qiime_study_2401:Plate6.66 Plate6.66:345 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92622 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 345 target_gene 16S rRNA study_center Cornell ERX520483 qiime_experiment_2401:1269528:90 qiime_experiment_2401:1269528:90 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502006 SAMEA2635652 qiime_study_2401:Plate15.34 Plate15.34:90 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64438 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 90 target_gene 16S rRNA study_center Cornell ERX520484 qiime_experiment_2401:1269530:427 qiime_experiment_2401:1269530:427 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502008 SAMEA2635654 qiime_study_2401:Plate7.72 Plate7.72:427 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 103002 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 427 target_gene 16S rRNA study_center Cornell ERX520485 qiime_experiment_2401:1269531:73 qiime_experiment_2401:1269531:73 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502009 SAMEA2635655 qiime_study_2401:Plate15.14 Plate15.14:73 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67054 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 73 target_gene 16S rRNA study_center Cornell ERX520486 qiime_experiment_2401:1269532:375 qiime_experiment_2401:1269532:375 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502010 SAMEA2635656 qiime_study_2401:Plate7.10 Plate7.10:375 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92180 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 375 target_gene 16S rRNA study_center Cornell ERX520577 qiime_experiment_2401:1269628:421 qiime_experiment_2401:1269628:421 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502106 SAMEA2635752 qiime_study_2401:Plate7.66 Plate7.66:421 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 108996 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 421 target_gene 16S rRNA study_center Cornell ERX520578 qiime_experiment_2401:1269629:105 qiime_experiment_2401:1269629:105 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502107 SAMEA2635753 qiime_study_2401:Plate15.52 Plate15.52:105 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62549 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 105 target_gene 16S rRNA study_center Cornell ERX520579 qiime_experiment_2401:1269630:154 qiime_experiment_2401:1269630:154 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502108 SAMEA2635754 qiime_study_2401:Plate16.24 Plate16.24:154 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 154 target_gene 16S rRNA study_center Cornell ERX520580 qiime_experiment_2401:1269631:71 qiime_experiment_2401:1269631:71 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502109 SAMEA2635755 qiime_study_2401:Plate15.12 Plate15.12:71 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71126 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 71 target_gene 16S rRNA study_center Cornell ERX520581 qiime_experiment_2401:1269632:378 qiime_experiment_2401:1269632:378 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502110 SAMEA2635756 qiime_study_2401:Plate7.13 Plate7.13:378 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84061 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 378 target_gene 16S rRNA study_center Cornell ERX520582 qiime_experiment_2401:1269633:320 qiime_experiment_2401:1269633:320 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502111 SAMEA2635757 qiime_study_2401:Plate6.4 Plate6.4:320 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68818 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 320 target_gene 16S rRNA study_center Cornell ERX520673 qiime_experiment_2401:1269735:39 qiime_experiment_2401:1269735:39 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502213 SAMEA2635859 qiime_study_2401:Plate14.61 Plate14.61:39 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 44442 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 39 target_gene 16S rRNA study_center Cornell ERX520674 qiime_experiment_2401:1269736:21 qiime_experiment_2401:1269736:21 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502214 SAMEA2635860 qiime_study_2401:Plate14.4 Plate14.4:21 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64773 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 21 target_gene 16S rRNA study_center Cornell ERX520675 qiime_experiment_2401:1269737:136 qiime_experiment_2401:1269737:136 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502215 SAMEA2635861 qiime_study_2401:Plate15.93 Plate15.93:136 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 30479 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 136 target_gene 16S rRNA study_center Cornell ERX520676 qiime_experiment_2401:1269738:400 qiime_experiment_2401:1269738:400 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502216 SAMEA2635862 qiime_study_2401:Plate7.38 Plate7.38:400 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63141 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 400 target_gene 16S rRNA study_center Cornell ERX520677 qiime_experiment_2401:1269739:387 qiime_experiment_2401:1269739:387 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502217 SAMEA2635863 qiime_study_2401:Plate7.23 Plate7.23:387 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70084 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 387 target_gene 16S rRNA study_center Cornell ERX520678 qiime_experiment_2401:1269740:415 qiime_experiment_2401:1269740:415 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502218 SAMEA2635864 qiime_study_2401:Plate7.6 Plate7.6:415 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77820 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 415 target_gene 16S rRNA study_center Cornell ERX520209 qiime_experiment_2401:1269797:362 qiime_experiment_2401:1269797:362 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501719 SAMEA2635365 qiime_study_2401:Plate6.81 Plate6.81:362 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73326 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 362 target_gene 16S rRNA study_center Cornell ERX520210 qiime_experiment_2401:1269798:452 qiime_experiment_2401:1269798:452 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501720 SAMEA2635366 qiime_study_2401:Plate8.15 Plate8.15:452 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86078 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 452 target_gene 16S rRNA study_center Cornell ERX520211 qiime_experiment_2401:1269800:230 qiime_experiment_2401:1269800:230 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501722 SAMEA2635368 qiime_study_2401:Plate5.32 Plate5.32:230 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76613 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 230 target_gene 16S rRNA study_center Cornell ERX520212 qiime_experiment_2401:1269801:429 qiime_experiment_2401:1269801:429 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501723 SAMEA2635369 qiime_study_2401:Plate7.74 Plate7.74:429 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 111822 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 429 target_gene 16S rRNA study_center Cornell ERX520213 qiime_experiment_2401:1269802:101 qiime_experiment_2401:1269802:101 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501724 SAMEA2635370 qiime_study_2401:Plate15.48 Plate15.48:101 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78232 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 101 target_gene 16S rRNA study_center Cornell ERX520214 qiime_experiment_2401:1269803:476 qiime_experiment_2401:1269803:476 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501725 SAMEA2635371 qiime_study_2401:Plate8.41 Plate8.41:476 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94483 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 476 target_gene 16S rRNA study_center Cornell ERX520305 qiime_experiment_2401:1269343:396 qiime_experiment_2401:1269343:396 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501821 SAMEA2635467 qiime_study_2401:Plate7.33 Plate7.33:396 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89364 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 396 target_gene 16S rRNA study_center Cornell ERX520306 qiime_experiment_2401:1269344:485 qiime_experiment_2401:1269344:485 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501822 SAMEA2635468 qiime_study_2401:Plate8.50 Plate8.50:485 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 269979 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 485 target_gene 16S rRNA study_center Cornell ERX520307 qiime_experiment_2401:1269345:132 qiime_experiment_2401:1269345:132 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501823 SAMEA2635469 qiime_study_2401:Plate15.88 Plate15.88:132 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 28535 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 132 target_gene 16S rRNA study_center Cornell ERX520308 qiime_experiment_2401:1269346:113 qiime_experiment_2401:1269346:113 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501824 SAMEA2635470 qiime_study_2401:Plate15.60 Plate15.60:113 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 57507 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 113 target_gene 16S rRNA study_center Cornell ERX520309 qiime_experiment_2401:1269348:77 qiime_experiment_2401:1269348:77 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501826 SAMEA2635472 qiime_study_2401:Plate15.18 Plate15.18:77 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70788 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 77 target_gene 16S rRNA study_center Cornell ERX520310 qiime_experiment_2401:1269349:402 qiime_experiment_2401:1269349:402 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501827 SAMEA2635473 qiime_study_2401:Plate7.4 Plate7.4:402 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 97023 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 402 target_gene 16S rRNA study_center Cornell ERX520391 qiime_experiment_2401:1269435:213 qiime_experiment_2401:1269435:213 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501913 SAMEA2635559 qiime_study_2401:Plate5.14 Plate5.14:213 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89099 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 213 target_gene 16S rRNA study_center Cornell ERX520392 qiime_experiment_2401:1269436:405 qiime_experiment_2401:1269436:405 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501914 SAMEA2635560 qiime_study_2401:Plate7.45 Plate7.45:405 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82652 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 405 target_gene 16S rRNA study_center Cornell ERX520393 qiime_experiment_2401:1269437:308 qiime_experiment_2401:1269437:308 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501915 SAMEA2635561 qiime_study_2401:Plate6.27 Plate6.27:308 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 55224 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 308 target_gene 16S rRNA study_center Cornell ERX520394 qiime_experiment_2401:1269438:122 qiime_experiment_2401:1269438:122 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501916 SAMEA2635562 qiime_study_2401:Plate15.74 Plate15.74:122 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67177 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 122 target_gene 16S rRNA study_center Cornell ERX520395 qiime_experiment_2401:1269439:65 qiime_experiment_2401:1269439:65 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501917 SAMEA2635563 qiime_study_2401:Plate14.94 Plate14.94:65 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68633 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 65 target_gene 16S rRNA study_center Cornell ERX520396 qiime_experiment_2401:1269440:19 qiime_experiment_2401:1269440:19 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501918 SAMEA2635564 qiime_study_2401:Plate14.37 Plate14.37:19 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67224 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 19 target_gene 16S rRNA study_center Cornell ERX520487 qiime_experiment_2401:1269533:523 qiime_experiment_2401:1269533:523 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502011 SAMEA2635657 qiime_study_2401:Plate9.44 Plate9.44:523 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58815 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 523 target_gene 16S rRNA study_center Cornell ERX520488 qiime_experiment_2401:1269534:422 qiime_experiment_2401:1269534:422 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502012 SAMEA2635658 qiime_study_2401:Plate7.67 Plate7.67:422 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92382 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 422 target_gene 16S rRNA study_center Cornell ERX520489 qiime_experiment_2401:1269535:299 qiime_experiment_2401:1269535:299 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502013 SAMEA2635659 qiime_study_2401:Plate6.18 Plate6.18:299 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76486 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 299 target_gene 16S rRNA study_center Cornell ERX520490 qiime_experiment_2401:1269536:278 qiime_experiment_2401:1269536:278 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502014 SAMEA2635660 qiime_study_2401:Plate5.82 Plate5.82:278 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64013 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 278 target_gene 16S rRNA study_center Cornell ERX520491 qiime_experiment_2401:1269537:261 qiime_experiment_2401:1269537:261 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502015 SAMEA2635661 qiime_study_2401:Plate5.66 Plate5.66:261 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76137 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 261 target_gene 16S rRNA study_center Cornell ERX520492 qiime_experiment_2401:1269538:447 qiime_experiment_2401:1269538:447 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502016 SAMEA2635662 qiime_study_2401:Plate7.93 Plate7.93:447 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87320 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 447 target_gene 16S rRNA study_center Cornell ERX520583 qiime_experiment_2401:1269634:41 qiime_experiment_2401:1269634:41 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502112 SAMEA2635758 qiime_study_2401:Plate14.63 Plate14.63:41 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 98083 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 41 target_gene 16S rRNA study_center Cornell ERX520584 qiime_experiment_2401:1269635:51 qiime_experiment_2401:1269635:51 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502113 SAMEA2635759 qiime_study_2401:Plate14.75 Plate14.75:51 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66078 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 51 target_gene 16S rRNA study_center Cornell ERX520585 qiime_experiment_2401:1269636:407 qiime_experiment_2401:1269636:407 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502114 SAMEA2635760 qiime_study_2401:Plate7.50 Plate7.50:407 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93836 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 407 target_gene 16S rRNA study_center Cornell ERX520586 qiime_experiment_2401:1269637:272 qiime_experiment_2401:1269637:272 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502115 SAMEA2635761 qiime_study_2401:Plate5.77 Plate5.77:272 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94805 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 272 target_gene 16S rRNA study_center Cornell ERX520587 qiime_experiment_2401:1269638:391 qiime_experiment_2401:1269638:391 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502116 SAMEA2635762 qiime_study_2401:Plate7.28 Plate7.28:391 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88725 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 391 target_gene 16S rRNA study_center Cornell ERX520588 qiime_experiment_2401:1269639:211 qiime_experiment_2401:1269639:211 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502117 SAMEA2635763 qiime_study_2401:Plate5.11 Plate5.11:211 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80633 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 211 target_gene 16S rRNA study_center Cornell ERX520679 qiime_experiment_2401:1269741:106 qiime_experiment_2401:1269741:106 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502219 SAMEA2635865 qiime_study_2401:Plate15.53 Plate15.53:106 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58852 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 106 target_gene 16S rRNA study_center Cornell ERX520680 qiime_experiment_2401:1269743:251 qiime_experiment_2401:1269743:251 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502221 SAMEA2635867 qiime_study_2401:Plate5.55 Plate5.55:251 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93127 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 251 target_gene 16S rRNA study_center Cornell ERX520681 qiime_experiment_2401:1269744:404 qiime_experiment_2401:1269744:404 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502222 SAMEA2635868 qiime_study_2401:Plate7.42 Plate7.42:404 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83320 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 404 target_gene 16S rRNA study_center Cornell ERX520682 qiime_experiment_2401:1269745:257 qiime_experiment_2401:1269745:257 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502223 SAMEA2635869 qiime_study_2401:Plate5.62 Plate5.62:257 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73353 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 257 target_gene 16S rRNA study_center Cornell ERX520683 qiime_experiment_2401:1269746:440 qiime_experiment_2401:1269746:440 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502224 SAMEA2635870 qiime_study_2401:Plate7.86 Plate7.86:440 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 99279 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 440 target_gene 16S rRNA study_center Cornell ERX520684 qiime_experiment_2401:1269747:8 qiime_experiment_2401:1269747:8 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502225 SAMEA2635871 qiime_study_2401:Plate14.20 Plate14.20:8 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73287 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 8 target_gene 16S rRNA study_center Cornell ERX520215 qiime_experiment_2401:1269804:546 qiime_experiment_2401:1269804:546 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501726 SAMEA2635372 qiime_study_2401:Plate9.73 Plate9.73:546 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 55545 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 546 target_gene 16S rRNA study_center Cornell ERX520216 qiime_experiment_2401:1269805:219 qiime_experiment_2401:1269805:219 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501727 SAMEA2635373 qiime_study_2401:Plate5.2 Plate5.2:219 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 95386 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 219 target_gene 16S rRNA study_center Cornell ERX520217 qiime_experiment_2401:1269806:152 qiime_experiment_2401:1269806:152 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501728 SAMEA2635374 qiime_study_2401:Plate16.22 Plate16.22:152 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 4 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 152 target_gene 16S rRNA study_center Cornell ERX520218 qiime_experiment_2401:1269807:37 qiime_experiment_2401:1269807:37 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501729 SAMEA2635375 qiime_study_2401:Plate14.6 Plate14.6:37 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 45439 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 37 target_gene 16S rRNA study_center Cornell ERX520219 qiime_experiment_2401:1269808:355 qiime_experiment_2401:1269808:355 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501730 SAMEA2635376 qiime_study_2401:Plate6.75 Plate6.75:355 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 103040 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 355 target_gene 16S rRNA study_center Cornell ERX520220 qiime_experiment_2401:1269809:99 qiime_experiment_2401:1269809:99 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501731 SAMEA2635377 qiime_study_2401:Plate15.44 Plate15.44:99 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77088 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 99 target_gene 16S rRNA study_center Cornell ERX520311 qiime_experiment_2401:1269350:61 qiime_experiment_2401:1269350:61 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501828 SAMEA2635474 qiime_study_2401:Plate14.89 Plate14.89:61 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66470 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 61 target_gene 16S rRNA study_center Cornell ERX520312 qiime_experiment_2401:1269351:555 qiime_experiment_2401:1269351:555 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501829 SAMEA2635475 qiime_study_2401:Plate9.96 Plate9.96:555 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88700 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 555 target_gene 16S rRNA study_center Cornell ERX520313 qiime_experiment_2401:1269352:369 qiime_experiment_2401:1269352:369 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501830 SAMEA2635476 qiime_study_2401:Plate6.89 Plate6.89:369 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90441 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 369 target_gene 16S rRNA study_center Cornell ERX520314 qiime_experiment_2401:1269353:10 qiime_experiment_2401:1269353:10 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501831 SAMEA2635477 qiime_study_2401:Plate14.24 Plate14.24:10 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 101929 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 10 target_gene 16S rRNA study_center Cornell ERX520315 qiime_experiment_2401:1269354:473 qiime_experiment_2401:1269354:473 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501832 SAMEA2635478 qiime_study_2401:Plate8.39 Plate8.39:473 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82162 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 473 target_gene 16S rRNA study_center Cornell ERX520316 qiime_experiment_2401:1269355:432 qiime_experiment_2401:1269355:432 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501833 SAMEA2635479 qiime_study_2401:Plate7.77 Plate7.77:432 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102971 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 432 target_gene 16S rRNA study_center Cornell ERX520397 qiime_experiment_2401:1269441:468 qiime_experiment_2401:1269441:468 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501919 SAMEA2635565 qiime_study_2401:Plate8.32 Plate8.32:468 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 91151 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 468 target_gene 16S rRNA study_center Cornell ERX520398 qiime_experiment_2401:1269442:42 qiime_experiment_2401:1269442:42 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501920 SAMEA2635566 qiime_study_2401:Plate14.64 Plate14.64:42 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78328 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 42 target_gene 16S rRNA study_center Cornell ERX520399 qiime_experiment_2401:1269443:428 qiime_experiment_2401:1269443:428 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501921 SAMEA2635567 qiime_study_2401:Plate7.73 Plate7.73:428 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 114254 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 428 target_gene 16S rRNA study_center Cornell ERX520400 qiime_experiment_2401:1269444:171 qiime_experiment_2401:1269444:171 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501922 SAMEA2635568 qiime_study_2401:Plate16.41 Plate16.41:171 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 3 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 171 target_gene 16S rRNA study_center Cornell ERX520401 qiime_experiment_2401:1269445:326 qiime_experiment_2401:1269445:326 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501923 SAMEA2635569 qiime_study_2401:Plate6.47 Plate6.47:326 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79340 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 326 target_gene 16S rRNA study_center Cornell ERX520402 qiime_experiment_2401:1269446:124 qiime_experiment_2401:1269446:124 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501924 SAMEA2635570 qiime_study_2401:Plate15.77 Plate15.77:124 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67258 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 124 target_gene 16S rRNA study_center Cornell ERX520493 qiime_experiment_2401:1269539:178 qiime_experiment_2401:1269539:178 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502017 SAMEA2635663 qiime_study_2401:Plate16.49 Plate16.49:178 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 5 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 178 target_gene 16S rRNA study_center Cornell ERX520494 qiime_experiment_2401:1269540:55 qiime_experiment_2401:1269540:55 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502018 SAMEA2635664 qiime_study_2401:Plate14.79 Plate14.79:55 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 52298 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 55 target_gene 16S rRNA study_center Cornell ERX520495 qiime_experiment_2401:1269541:148 qiime_experiment_2401:1269541:148 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502019 SAMEA2635665 qiime_study_2401:Plate16.18 Plate16.18:148 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 148 target_gene 16S rRNA study_center Cornell ERX520496 qiime_experiment_2401:1269542:424 qiime_experiment_2401:1269542:424 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502020 SAMEA2635666 qiime_study_2401:Plate7.7 Plate7.7:424 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 98311 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 424 target_gene 16S rRNA study_center Cornell ERX520497 qiime_experiment_2401:1269543:262 qiime_experiment_2401:1269543:262 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502021 SAMEA2635667 qiime_study_2401:Plate5.67 Plate5.67:262 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68825 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 262 target_gene 16S rRNA study_center Cornell ERX520498 qiime_experiment_2401:1269544:223 qiime_experiment_2401:1269544:223 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502022 SAMEA2635668 qiime_study_2401:Plate5.25 Plate5.25:223 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93167 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 223 target_gene 16S rRNA study_center Cornell ERX520589 qiime_experiment_2401:1269640:524 qiime_experiment_2401:1269640:524 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502118 SAMEA2635764 qiime_study_2401:Plate9.45 Plate9.45:524 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73612 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 524 target_gene 16S rRNA study_center Cornell ERX520590 qiime_experiment_2401:1269641:24 qiime_experiment_2401:1269641:24 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502119 SAMEA2635765 qiime_study_2401:Plate14.43 Plate14.43:24 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83966 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 24 target_gene 16S rRNA study_center Cornell ERX520591 qiime_experiment_2401:1269642:46 qiime_experiment_2401:1269642:46 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502120 SAMEA2635766 qiime_study_2401:Plate14.7 Plate14.7:46 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72280 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 46 target_gene 16S rRNA study_center Cornell ERX520592 qiime_experiment_2401:1269643:35 qiime_experiment_2401:1269643:35 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502121 SAMEA2635767 qiime_study_2401:Plate14.58 Plate14.58:35 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70728 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 35 target_gene 16S rRNA study_center Cornell ERX520593 qiime_experiment_2401:1269645:484 qiime_experiment_2401:1269645:484 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502123 SAMEA2635769 qiime_study_2401:Plate8.5 Plate8.5:484 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83817 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 484 target_gene 16S rRNA study_center Cornell ERX520594 qiime_experiment_2401:1269646:460 qiime_experiment_2401:1269646:460 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502124 SAMEA2635770 qiime_study_2401:Plate8.24 Plate8.24:460 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71107 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 460 target_gene 16S rRNA study_center Cornell ERX520685 qiime_experiment_2401:1269748:423 qiime_experiment_2401:1269748:423 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502226 SAMEA2635872 qiime_study_2401:Plate7.69 Plate7.69:423 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 96698 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 423 target_gene 16S rRNA study_center Cornell ERX520686 qiime_experiment_2401:1269749:198 qiime_experiment_2401:1269749:198 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502227 SAMEA2635873 qiime_study_2401:Plate16.67 Plate16.67:198 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 163716 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 198 target_gene 16S rRNA study_center Cornell ERX520687 qiime_experiment_2401:1269750:519 qiime_experiment_2401:1269750:519 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502228 SAMEA2635874 qiime_study_2401:Plate9.37 Plate9.37:519 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59491 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 519 target_gene 16S rRNA study_center Cornell ERX520688 qiime_experiment_2401:1269751:451 qiime_experiment_2401:1269751:451 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502229 SAMEA2635875 qiime_study_2401:Plate8.14 Plate8.14:451 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74544 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 451 target_gene 16S rRNA study_center Cornell ERX520689 qiime_experiment_2401:1269752:216 qiime_experiment_2401:1269752:216 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502230 SAMEA2635876 qiime_study_2401:Plate5.17 Plate5.17:216 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79367 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 216 target_gene 16S rRNA study_center Cornell ERX520690 qiime_experiment_2401:1269753:343 qiime_experiment_2401:1269753:343 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502231 SAMEA2635877 qiime_study_2401:Plate6.64 Plate6.64:343 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69955 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 343 target_gene 16S rRNA study_center Cornell ERX520221 qiime_experiment_2401:1269810:526 qiime_experiment_2401:1269810:526 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501732 SAMEA2635378 qiime_study_2401:Plate9.49 Plate9.49:526 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 48472 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 526 target_gene 16S rRNA study_center Cornell ERX520222 qiime_experiment_2401:1269811:247 qiime_experiment_2401:1269811:247 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501733 SAMEA2635379 qiime_study_2401:Plate5.51 Plate5.51:247 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77431 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 247 target_gene 16S rRNA study_center Cornell ERX520223 qiime_experiment_2401:1269813:337 qiime_experiment_2401:1269813:337 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501735 SAMEA2635381 qiime_study_2401:Plate6.57 Plate6.57:337 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78760 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 337 target_gene 16S rRNA study_center Cornell ERX520224 qiime_experiment_2401:1269814:446 qiime_experiment_2401:1269814:446 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501736 SAMEA2635382 qiime_study_2401:Plate7.92 Plate7.92:446 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 105803 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 446 target_gene 16S rRNA study_center Cornell ERX520225 qiime_experiment_2401:1269815:186 qiime_experiment_2401:1269815:186 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501737 SAMEA2635383 qiime_study_2401:Plate16.56 Plate16.56:186 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 3 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 186 target_gene 16S rRNA study_center Cornell ERX520226 qiime_experiment_2401:1269816:232 qiime_experiment_2401:1269816:232 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501738 SAMEA2635384 qiime_study_2401:Plate5.34 Plate5.34:232 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74602 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 232 target_gene 16S rRNA study_center Cornell ERX520317 qiime_experiment_2401:1269356:505 qiime_experiment_2401:1269356:505 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501834 SAMEA2635480 qiime_study_2401:Plate9.2 Plate9.2:505 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 104860 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 505 target_gene 16S rRNA study_center Cornell ERX520318 qiime_experiment_2401:1269357:80 qiime_experiment_2401:1269357:80 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501835 SAMEA2635481 qiime_study_2401:Plate15.20 Plate15.20:80 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59687 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 80 target_gene 16S rRNA study_center Cornell ERX520319 qiime_experiment_2401:1269358:503 qiime_experiment_2401:1269358:503 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501836 SAMEA2635482 qiime_study_2401:Plate9.1 Plate9.1:503 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102213 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 503 target_gene 16S rRNA study_center Cornell ERX520320 qiime_experiment_2401:1269359:86 qiime_experiment_2401:1269359:86 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501837 SAMEA2635483 qiime_study_2401:Plate15.29 Plate15.29:86 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82102 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 86 target_gene 16S rRNA study_center Cornell ERX520321 qiime_experiment_2401:1269360:128 qiime_experiment_2401:1269360:128 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501838 SAMEA2635484 qiime_study_2401:Plate15.81 Plate15.81:128 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58700 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 128 target_gene 16S rRNA study_center Cornell ERX520322 qiime_experiment_2401:1269361:371 qiime_experiment_2401:1269361:371 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501839 SAMEA2635485 qiime_study_2401:Plate6.90 Plate6.90:371 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87371 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 371 target_gene 16S rRNA study_center Cornell ERX520403 qiime_experiment_2401:1269447:274 qiime_experiment_2401:1269447:274 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501925 SAMEA2635571 qiime_study_2401:Plate5.79 Plate5.79:274 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74888 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 274 target_gene 16S rRNA study_center Cornell ERX520404 qiime_experiment_2401:1269448:342 qiime_experiment_2401:1269448:342 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501926 SAMEA2635572 qiime_study_2401:Plate6.62 Plate6.62:342 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65998 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 342 target_gene 16S rRNA study_center Cornell ERX520405 qiime_experiment_2401:1269449:514 qiime_experiment_2401:1269449:514 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501927 SAMEA2635573 qiime_study_2401:Plate9.30 Plate9.30:514 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90540 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 514 target_gene 16S rRNA study_center Cornell ERX520406 qiime_experiment_2401:1269450:163 qiime_experiment_2401:1269450:163 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501928 SAMEA2635574 qiime_study_2401:Plate16.32 Plate16.32:163 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 163 target_gene 16S rRNA study_center Cornell ERX520407 qiime_experiment_2401:1269451:455 qiime_experiment_2401:1269451:455 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501929 SAMEA2635575 qiime_study_2401:Plate8.18 Plate8.18:455 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 109326 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 455 target_gene 16S rRNA study_center Cornell ERX520408 qiime_experiment_2401:1269452:532 qiime_experiment_2401:1269452:532 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501930 SAMEA2635576 qiime_study_2401:Plate9.57 Plate9.57:532 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 52225 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 532 target_gene 16S rRNA study_center Cornell ERX520499 qiime_experiment_2401:1269545:527 qiime_experiment_2401:1269545:527 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502023 SAMEA2635669 qiime_study_2401:Plate9.5 Plate9.5:527 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102363 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 527 target_gene 16S rRNA study_center Cornell ERX520500 qiime_experiment_2401:1269546:307 qiime_experiment_2401:1269546:307 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502024 SAMEA2635670 qiime_study_2401:Plate6.25 Plate6.25:307 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81843 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 307 target_gene 16S rRNA study_center Cornell ERX520501 qiime_experiment_2401:1269547:504 qiime_experiment_2401:1269547:504 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502025 SAMEA2635671 qiime_study_2401:Plate9.15 Plate9.15:504 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93587 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 504 target_gene 16S rRNA study_center Cornell ERX520502 qiime_experiment_2401:1269548:81 qiime_experiment_2401:1269548:81 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502026 SAMEA2635672 qiime_study_2401:Plate15.21 Plate15.21:81 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 31883 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 81 target_gene 16S rRNA study_center Cornell ERX520503 qiime_experiment_2401:1269549:102 qiime_experiment_2401:1269549:102 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502027 SAMEA2635673 qiime_study_2401:Plate15.49 Plate15.49:102 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 61478 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 102 target_gene 16S rRNA study_center Cornell ERX520504 qiime_experiment_2401:1269550:554 qiime_experiment_2401:1269550:554 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502028 SAMEA2635674 qiime_study_2401:Plate9.91 Plate9.91:554 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69821 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 554 target_gene 16S rRNA study_center Cornell ERX520595 qiime_experiment_2401:1269647:30 qiime_experiment_2401:1269647:30 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502125 SAMEA2635771 qiime_study_2401:Plate14.51 Plate14.51:30 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86346 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 30 target_gene 16S rRNA study_center Cornell ERX520596 qiime_experiment_2401:1269648:380 qiime_experiment_2401:1269648:380 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502126 SAMEA2635772 qiime_study_2401:Plate7.15 Plate7.15:380 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83489 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 380 target_gene 16S rRNA study_center Cornell ERX520597 qiime_experiment_2401:1269649:218 qiime_experiment_2401:1269649:218 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502127 SAMEA2635773 qiime_study_2401:Plate5.19 Plate5.19:218 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77545 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 218 target_gene 16S rRNA study_center Cornell ERX520598 qiime_experiment_2401:1269650:50 qiime_experiment_2401:1269650:50 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502128 SAMEA2635774 qiime_study_2401:Plate14.74 Plate14.74:50 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70888 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 50 target_gene 16S rRNA study_center Cornell ERX520599 qiime_experiment_2401:1269651:490 qiime_experiment_2401:1269651:490 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502129 SAMEA2635775 qiime_study_2401:Plate8.59 Plate8.59:490 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76614 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 490 target_gene 16S rRNA study_center Cornell ERX520600 qiime_experiment_2401:1269652:137 qiime_experiment_2401:1269652:137 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502130 SAMEA2635776 qiime_study_2401:Plate15.94 Plate15.94:137 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 44699 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 137 target_gene 16S rRNA study_center Cornell ERX520691 qiime_experiment_2401:1269755:347 qiime_experiment_2401:1269755:347 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502233 SAMEA2635879 qiime_study_2401:Plate6.68 Plate6.68:347 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59534 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 347 target_gene 16S rRNA study_center Cornell ERX520692 qiime_experiment_2401:1269756:133 qiime_experiment_2401:1269756:133 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502234 SAMEA2635880 qiime_study_2401:Plate15.89 Plate15.89:133 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64056 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 133 target_gene 16S rRNA study_center Cornell ERX520693 qiime_experiment_2401:1269757:303 qiime_experiment_2401:1269757:303 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502235 SAMEA2635881 qiime_study_2401:Plate6.21 Plate6.21:303 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71711 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 303 target_gene 16S rRNA study_center Cornell ERX520694 qiime_experiment_2401:1269758:364 qiime_experiment_2401:1269758:364 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502236 SAMEA2635882 qiime_study_2401:Plate6.83 Plate6.83:364 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86114 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 364 target_gene 16S rRNA study_center Cornell ERX520695 qiime_experiment_2401:1269759:15 qiime_experiment_2401:1269759:15 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502237 SAMEA2635883 qiime_study_2401:Plate14.31 Plate14.31:15 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73414 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 15 target_gene 16S rRNA study_center Cornell ERX520227 qiime_experiment_2401:1269817:269 qiime_experiment_2401:1269817:269 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501739 SAMEA2635385 qiime_study_2401:Plate5.74 Plate5.74:269 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88282 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 269 target_gene 16S rRNA study_center Cornell ERX520228 qiime_experiment_2401:1269818:480 qiime_experiment_2401:1269818:480 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501740 SAMEA2635386 qiime_study_2401:Plate8.45 Plate8.45:480 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 113616 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 480 target_gene 16S rRNA study_center Cornell ERX520229 qiime_experiment_2401:1269819:187 qiime_experiment_2401:1269819:187 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501741 SAMEA2635387 qiime_study_2401:Plate16.57 Plate16.57:187 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 187 target_gene 16S rRNA study_center Cornell ERX520230 qiime_experiment_2401:1269820:353 qiime_experiment_2401:1269820:353 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501742 SAMEA2635388 qiime_study_2401:Plate6.73 Plate6.73:353 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 97882 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 353 target_gene 16S rRNA study_center Cornell ERX520231 qiime_experiment_2401:1269821:403 qiime_experiment_2401:1269821:403 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501743 SAMEA2635389 qiime_study_2401:Plate7.41 Plate7.41:403 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78640 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 403 target_gene 16S rRNA study_center Cornell ERX520232 qiime_experiment_2401:1269822:36 qiime_experiment_2401:1269822:36 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501744 SAMEA2635390 qiime_study_2401:Plate14.59 Plate14.59:36 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65942 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 36 target_gene 16S rRNA study_center Cornell ERX520323 qiime_experiment_2401:1269362:143 qiime_experiment_2401:1269362:143 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501840 SAMEA2635486 qiime_study_2401:Plate16.12 Plate16.12:143 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 143 target_gene 16S rRNA study_center Cornell ERX520324 qiime_experiment_2401:1269363:248 qiime_experiment_2401:1269363:248 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501841 SAMEA2635487 qiime_study_2401:Plate5.52 Plate5.52:248 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90362 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 248 target_gene 16S rRNA study_center Cornell ERX520325 qiime_experiment_2401:1269364:227 qiime_experiment_2401:1269364:227 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501842 SAMEA2635488 qiime_study_2401:Plate5.3 Plate5.3:227 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94482 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 227 target_gene 16S rRNA study_center Cornell ERX520326 qiime_experiment_2401:1269365:341 qiime_experiment_2401:1269365:341 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501843 SAMEA2635489 qiime_study_2401:Plate6.61 Plate6.61:341 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64268 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 341 target_gene 16S rRNA study_center Cornell ERX520327 qiime_experiment_2401:1269366:386 qiime_experiment_2401:1269366:386 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501844 SAMEA2635490 qiime_study_2401:Plate7.21 Plate7.21:386 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 79090 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 386 target_gene 16S rRNA study_center Cornell ERX520328 qiime_experiment_2401:1269367:356 qiime_experiment_2401:1269367:356 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501845 SAMEA2635491 qiime_study_2401:Plate6.76 Plate6.76:356 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74458 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 356 target_gene 16S rRNA study_center Cornell ERX520409 qiime_experiment_2401:1269453:68 qiime_experiment_2401:1269453:68 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501931 SAMEA2635577 qiime_study_2401:Plate15.1 Plate15.1:68 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65544 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 68 target_gene 16S rRNA study_center Cornell ERX520410 qiime_experiment_2401:1269454:392 qiime_experiment_2401:1269454:392 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501932 SAMEA2635578 qiime_study_2401:Plate7.29 Plate7.29:392 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83053 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 392 target_gene 16S rRNA study_center Cornell ERX520411 qiime_experiment_2401:1269455:346 qiime_experiment_2401:1269455:346 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501933 SAMEA2635579 qiime_study_2401:Plate6.67 Plate6.67:346 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63565 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 346 target_gene 16S rRNA study_center Cornell ERX520412 qiime_experiment_2401:1269457:496 qiime_experiment_2401:1269457:496 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501935 SAMEA2635581 qiime_study_2401:Plate8.75 Plate8.75:496 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85675 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 496 target_gene 16S rRNA study_center Cornell ERX520413 qiime_experiment_2401:1269458:361 qiime_experiment_2401:1269458:361 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501936 SAMEA2635582 qiime_study_2401:Plate6.80 Plate6.80:361 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77739 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 361 target_gene 16S rRNA study_center Cornell ERX520414 qiime_experiment_2401:1269459:49 qiime_experiment_2401:1269459:49 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501937 SAMEA2635583 qiime_study_2401:Plate14.73 Plate14.73:49 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85733 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 49 target_gene 16S rRNA study_center Cornell ERX520505 qiime_experiment_2401:1269551:333 qiime_experiment_2401:1269551:333 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502029 SAMEA2635675 qiime_study_2401:Plate6.53 Plate6.53:333 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62619 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 333 target_gene 16S rRNA study_center Cornell ERX520506 qiime_experiment_2401:1269552:54 qiime_experiment_2401:1269552:54 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502030 SAMEA2635676 qiime_study_2401:Plate14.78 Plate14.78:54 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 40361 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 54 target_gene 16S rRNA study_center Cornell ERX520507 qiime_experiment_2401:1269553:110 qiime_experiment_2401:1269553:110 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502031 SAMEA2635677 qiime_study_2401:Plate15.57 Plate15.57:110 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58256 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 110 target_gene 16S rRNA study_center Cornell ERX520508 qiime_experiment_2401:1269554:513 qiime_experiment_2401:1269554:513 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502032 SAMEA2635678 qiime_study_2401:Plate9.3 Plate9.3:513 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74001 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 513 target_gene 16S rRNA study_center Cornell ERX520509 qiime_experiment_2401:1269555:541 qiime_experiment_2401:1269555:541 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502033 SAMEA2635679 qiime_study_2401:Plate9.66 Plate9.66:541 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 102191 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 541 target_gene 16S rRNA study_center Cornell ERX520510 qiime_experiment_2401:1269556:134 qiime_experiment_2401:1269556:134 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502034 SAMEA2635680 qiime_study_2401:Plate15.9 Plate15.9:134 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63360 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 134 target_gene 16S rRNA study_center Cornell ERX520601 qiime_experiment_2401:1269654:366 qiime_experiment_2401:1269654:366 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502132 SAMEA2635778 qiime_study_2401:Plate6.86 Plate6.86:366 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67063 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 366 target_gene 16S rRNA study_center Cornell ERX520602 qiime_experiment_2401:1269655:316 qiime_experiment_2401:1269655:316 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502133 SAMEA2635779 qiime_study_2401:Plate6.35 Plate6.35:316 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74599 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 316 target_gene 16S rRNA study_center Cornell ERX520603 qiime_experiment_2401:1269656:477 qiime_experiment_2401:1269656:477 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502134 SAMEA2635780 qiime_study_2401:Plate8.42 Plate8.42:477 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92392 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 477 target_gene 16S rRNA study_center Cornell ERX520604 qiime_experiment_2401:1269657:91 qiime_experiment_2401:1269657:91 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502135 SAMEA2635781 qiime_study_2401:Plate15.35 Plate15.35:91 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64476 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 91 target_gene 16S rRNA study_center Cornell ERX520605 qiime_experiment_2401:1269658:463 qiime_experiment_2401:1269658:463 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502136 SAMEA2635782 qiime_study_2401:Plate8.27 Plate8.27:463 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93708 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 463 target_gene 16S rRNA study_center Cornell ERX520606 qiime_experiment_2401:1269659:234 qiime_experiment_2401:1269659:234 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502137 SAMEA2635783 qiime_study_2401:Plate5.36 Plate5.36:234 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90672 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 234 target_gene 16S rRNA study_center Cornell ERX520233 qiime_experiment_2401:1269823:241 qiime_experiment_2401:1269823:241 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501745 SAMEA2635391 qiime_study_2401:Plate5.44 Plate5.44:241 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68654 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 241 target_gene 16S rRNA study_center Cornell ERX520234 qiime_experiment_2401:1269824:510 qiime_experiment_2401:1269824:510 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501746 SAMEA2635392 qiime_study_2401:Plate9.26 Plate9.26:510 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 101814 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 510 target_gene 16S rRNA study_center Cornell ERX520235 qiime_experiment_2401:1269825:445 qiime_experiment_2401:1269825:445 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501747 SAMEA2635393 qiime_study_2401:Plate7.91 Plate7.91:445 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 98084 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 445 target_gene 16S rRNA study_center Cornell ERX520236 qiime_experiment_2401:1269826:252 qiime_experiment_2401:1269826:252 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501748 SAMEA2635394 qiime_study_2401:Plate5.56 Plate5.56:252 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76730 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 252 target_gene 16S rRNA study_center Cornell ERX520237 qiime_experiment_2401:1269827:550 qiime_experiment_2401:1269827:550 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501749 SAMEA2635395 qiime_study_2401:Plate9.81 Plate9.81:550 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66823 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 550 target_gene 16S rRNA study_center Cornell ERX520238 qiime_experiment_2401:1269828:322 qiime_experiment_2401:1269828:322 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501750 SAMEA2635396 qiime_study_2401:Plate6.41 Plate6.41:322 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72123 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 322 target_gene 16S rRNA study_center Cornell ERX520330 qiime_experiment_2401:1269369:277 qiime_experiment_2401:1269369:277 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501847 SAMEA2635493 qiime_study_2401:Plate5.81 Plate5.81:277 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69248 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 277 target_gene 16S rRNA study_center Cornell ERX520331 qiime_experiment_2401:1269370:461 qiime_experiment_2401:1269370:461 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501848 SAMEA2635494 qiime_study_2401:Plate8.25 Plate8.25:461 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 138797 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 461 target_gene 16S rRNA study_center Cornell ERX520329 qiime_experiment_2401:1269368:17 qiime_experiment_2401:1269368:17 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501846 SAMEA2635492 qiime_study_2401:Plate14.34 Plate14.34:17 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 46133 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 17 target_gene 16S rRNA study_center Cornell ERX520332 qiime_experiment_2401:1269372:244 qiime_experiment_2401:1269372:244 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501850 SAMEA2635496 qiime_study_2401:Plate5.48 Plate5.48:244 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82485 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 244 target_gene 16S rRNA study_center Cornell ERX520333 qiime_experiment_2401:1269373:321 qiime_experiment_2401:1269373:321 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501851 SAMEA2635497 qiime_study_2401:Plate6.40 Plate6.40:321 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68562 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 321 target_gene 16S rRNA study_center Cornell ERX520334 qiime_experiment_2401:1269374:350 qiime_experiment_2401:1269374:350 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501852 SAMEA2635498 qiime_study_2401:Plate6.70 Plate6.70:350 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87811 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 350 target_gene 16S rRNA study_center Cornell ERX520416 qiime_experiment_2401:1269461:53 qiime_experiment_2401:1269461:53 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501939 SAMEA2635585 qiime_study_2401:Plate14.77 Plate14.77:53 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69074 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 53 target_gene 16S rRNA study_center Cornell ERX520417 qiime_experiment_2401:1269462:56 qiime_experiment_2401:1269462:56 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501940 SAMEA2635586 qiime_study_2401:Plate14.8 Plate14.8:56 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62598 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 56 target_gene 16S rRNA study_center Cornell ERX520415 qiime_experiment_2401:1269460:5 qiime_experiment_2401:1269460:5 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501938 SAMEA2635584 qiime_study_2401:Plate14.18 Plate14.18:5 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 48779 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 5 target_gene 16S rRNA study_center Cornell ERX520418 qiime_experiment_2401:1269463:76 qiime_experiment_2401:1269463:76 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501941 SAMEA2635587 qiime_study_2401:Plate15.17 Plate15.17:76 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71081 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 76 target_gene 16S rRNA study_center Cornell ERX520419 qiime_experiment_2401:1269464:111 qiime_experiment_2401:1269464:111 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501942 SAMEA2635588 qiime_study_2401:Plate15.59 Plate15.59:111 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64175 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 111 target_gene 16S rRNA study_center Cornell ERX520420 qiime_experiment_2401:1269465:370 qiime_experiment_2401:1269465:370 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501943 SAMEA2635589 qiime_study_2401:Plate6.9 Plate6.9:370 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93194 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 370 target_gene 16S rRNA study_center Cornell ERX520511 qiime_experiment_2401:1269557:304 qiime_experiment_2401:1269557:304 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502035 SAMEA2635681 qiime_study_2401:Plate6.22 Plate6.22:304 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 45051 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 304 target_gene 16S rRNA study_center Cornell ERX520512 qiime_experiment_2401:1269558:121 qiime_experiment_2401:1269558:121 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502036 SAMEA2635682 qiime_study_2401:Plate15.73 Plate15.73:121 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64198 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 121 target_gene 16S rRNA study_center Cornell ERX520514 qiime_experiment_2401:1269561:528 qiime_experiment_2401:1269561:528 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502039 SAMEA2635685 qiime_study_2401:Plate9.50 Plate9.50:528 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 72289 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 528 target_gene 16S rRNA study_center Cornell ERX520515 qiime_experiment_2401:1269562:292 qiime_experiment_2401:1269562:292 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502040 SAMEA2635686 qiime_study_2401:Plate6.10 Plate6.10:292 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78780 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 292 target_gene 16S rRNA study_center Cornell ERX520513 qiime_experiment_2401:1269560:237 qiime_experiment_2401:1269560:237 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502038 SAMEA2635684 qiime_study_2401:Plate5.4 Plate5.4:237 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92517 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 237 target_gene 16S rRNA study_center Cornell ERX520516 qiime_experiment_2401:1269563:327 qiime_experiment_2401:1269563:327 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502041 SAMEA2635687 qiime_study_2401:Plate6.48 Plate6.48:327 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63884 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 327 target_gene 16S rRNA study_center Cornell ERX520607 qiime_experiment_2401:1269660:284 qiime_experiment_2401:1269660:284 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502138 SAMEA2635784 qiime_study_2401:Plate5.89 Plate5.89:284 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65815 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 284 target_gene 16S rRNA study_center Cornell ERX520608 qiime_experiment_2401:1269661:108 qiime_experiment_2401:1269661:108 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502139 SAMEA2635785 qiime_study_2401:Plate15.55 Plate15.55:108 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58383 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 108 target_gene 16S rRNA study_center Cornell ERX520609 qiime_experiment_2401:1269662:344 qiime_experiment_2401:1269662:344 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502140 SAMEA2635786 qiime_study_2401:Plate6.65 Plate6.65:344 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75637 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 344 target_gene 16S rRNA study_center Cornell ERX520610 qiime_experiment_2401:1269663:442 qiime_experiment_2401:1269663:442 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502141 SAMEA2635787 qiime_study_2401:Plate7.88 Plate7.88:442 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77077 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 442 target_gene 16S rRNA study_center Cornell ERX520611 qiime_experiment_2401:1269665:552 qiime_experiment_2401:1269665:552 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502143 SAMEA2635789 qiime_study_2401:Plate9.87 Plate9.87:552 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59179 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 552 target_gene 16S rRNA study_center Cornell ERX520612 qiime_experiment_2401:1269666:544 qiime_experiment_2401:1269666:544 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502144 SAMEA2635790 qiime_study_2401:Plate9.71 Plate9.71:544 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75440 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 544 target_gene 16S rRNA study_center Cornell ERX520239 qiime_experiment_2401:1269829:131 qiime_experiment_2401:1269829:131 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501751 SAMEA2635397 qiime_study_2401:Plate15.87 Plate15.87:131 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 42843 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 131 target_gene 16S rRNA study_center Cornell ERX520240 qiime_experiment_2401:1269830:489 qiime_experiment_2401:1269830:489 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501752 SAMEA2635398 qiime_study_2401:Plate8.54 Plate8.54:489 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 175332 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 489 target_gene 16S rRNA study_center Cornell ERX520241 qiime_experiment_2401:1269831:499 qiime_experiment_2401:1269831:499 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501753 SAMEA2635399 qiime_study_2401:Plate8.84 Plate8.84:499 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 153742 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 499 target_gene 16S rRNA study_center Cornell ERX520242 qiime_experiment_2401:1269832:409 qiime_experiment_2401:1269832:409 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501754 SAMEA2635400 qiime_study_2401:Plate7.52 Plate7.52:409 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83445 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 409 target_gene 16S rRNA study_center Cornell ERX520243 qiime_experiment_2401:1269833:270 qiime_experiment_2401:1269833:270 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501755 SAMEA2635401 qiime_study_2401:Plate5.75 Plate5.75:270 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86085 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 270 target_gene 16S rRNA study_center Cornell ERX520244 qiime_experiment_2401:1269834:225 qiime_experiment_2401:1269834:225 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501756 SAMEA2635402 qiime_study_2401:Plate5.28 Plate5.28:225 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83671 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 225 target_gene 16S rRNA study_center Cornell ERX520335 qiime_experiment_2401:1269375:273 qiime_experiment_2401:1269375:273 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501853 SAMEA2635499 qiime_study_2401:Plate5.78 Plate5.78:273 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70465 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 273 target_gene 16S rRNA study_center Cornell ERX520336 qiime_experiment_2401:1269376:458 qiime_experiment_2401:1269376:458 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501854 SAMEA2635500 qiime_study_2401:Plate8.20 Plate8.20:458 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80517 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 458 target_gene 16S rRNA study_center Cornell ERX520337 qiime_experiment_2401:1269377:549 qiime_experiment_2401:1269377:549 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501855 SAMEA2635501 qiime_study_2401:Plate9.79 Plate9.79:549 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 88506 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 549 target_gene 16S rRNA study_center Cornell ERX520338 qiime_experiment_2401:1269378:508 qiime_experiment_2401:1269378:508 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501856 SAMEA2635502 qiime_study_2401:Plate9.24 Plate9.24:508 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89780 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 508 target_gene 16S rRNA study_center Cornell ERX520339 qiime_experiment_2401:1269379:250 qiime_experiment_2401:1269379:250 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501857 SAMEA2635503 qiime_study_2401:Plate5.54 Plate5.54:250 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81661 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 250 target_gene 16S rRNA study_center Cornell ERX520340 qiime_experiment_2401:1269381:182 qiime_experiment_2401:1269381:182 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501859 SAMEA2635505 qiime_study_2401:Plate16.52 Plate16.52:182 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 182 target_gene 16S rRNA study_center Cornell ERX520421 qiime_experiment_2401:1269466:228 qiime_experiment_2401:1269466:228 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501944 SAMEA2635590 qiime_study_2401:Plate5.30 Plate5.30:228 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69756 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 228 target_gene 16S rRNA study_center Cornell ERX520422 qiime_experiment_2401:1269467:454 qiime_experiment_2401:1269467:454 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501945 SAMEA2635591 qiime_study_2401:Plate8.17 Plate8.17:454 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 129649 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 454 target_gene 16S rRNA study_center Cornell ERX520424 qiime_experiment_2401:1269469:433 qiime_experiment_2401:1269469:433 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501947 SAMEA2635593 qiime_study_2401:Plate7.78 Plate7.78:433 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 97269 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 433 target_gene 16S rRNA study_center Cornell ERX520425 qiime_experiment_2401:1269470:330 qiime_experiment_2401:1269470:330 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501948 SAMEA2635594 qiime_study_2401:Plate6.50 Plate6.50:330 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84794 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 330 target_gene 16S rRNA study_center Cornell ERX520423 qiime_experiment_2401:1269468:388 qiime_experiment_2401:1269468:388 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501946 SAMEA2635592 qiime_study_2401:Plate7.24 Plate7.24:388 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81732 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 388 target_gene 16S rRNA study_center Cornell ERX520426 qiime_experiment_2401:1269471:411 qiime_experiment_2401:1269471:411 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501949 SAMEA2635595 qiime_study_2401:Plate7.54 Plate7.54:411 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78376 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 411 target_gene 16S rRNA study_center Cornell ERX520517 qiime_experiment_2401:1269564:231 qiime_experiment_2401:1269564:231 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502042 SAMEA2635688 qiime_study_2401:Plate5.33 Plate5.33:231 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92291 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 231 target_gene 16S rRNA study_center Cornell ERX520518 qiime_experiment_2401:1269565:48 qiime_experiment_2401:1269565:48 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502043 SAMEA2635689 qiime_study_2401:Plate14.72 Plate14.72:48 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82990 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 48 target_gene 16S rRNA study_center Cornell ERX520519 qiime_experiment_2401:1269566:130 qiime_experiment_2401:1269566:130 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502044 SAMEA2635690 qiime_study_2401:Plate15.84 Plate15.84:130 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58390 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 130 target_gene 16S rRNA study_center Cornell ERX520520 qiime_experiment_2401:1269567:193 qiime_experiment_2401:1269567:193 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502045 SAMEA2635691 qiime_study_2401:Plate16.62 Plate16.62:193 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 3 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 193 target_gene 16S rRNA study_center Cornell ERX520521 qiime_experiment_2401:1269568:466 qiime_experiment_2401:1269568:466 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502046 SAMEA2635692 qiime_study_2401:Plate8.30 Plate8.30:466 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 98717 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 466 target_gene 16S rRNA study_center Cornell ERX520522 qiime_experiment_2401:1269569:98 qiime_experiment_2401:1269569:98 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502047 SAMEA2635693 qiime_study_2401:Plate15.42 Plate15.42:98 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 56938 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 98 target_gene 16S rRNA study_center Cornell ERX520613 qiime_experiment_2401:1269667:238 qiime_experiment_2401:1269667:238 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502145 SAMEA2635791 qiime_study_2401:Plate5.40 Plate5.40:238 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83514 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 238 target_gene 16S rRNA study_center Cornell ERX520614 qiime_experiment_2401:1269668:483 qiime_experiment_2401:1269668:483 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502146 SAMEA2635792 qiime_study_2401:Plate8.49 Plate8.49:483 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90432 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 483 target_gene 16S rRNA study_center Cornell ERX520615 qiime_experiment_2401:1269669:374 qiime_experiment_2401:1269669:374 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502147 SAMEA2635793 qiime_study_2401:Plate7.1 Plate7.1:374 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89894 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 374 target_gene 16S rRNA study_center Cornell ERX520616 qiime_experiment_2401:1269670:352 qiime_experiment_2401:1269670:352 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502148 SAMEA2635794 qiime_study_2401:Plate6.72 Plate6.72:352 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78702 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 352 target_gene 16S rRNA study_center Cornell ERX520617 qiime_experiment_2401:1269671:190 qiime_experiment_2401:1269671:190 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502149 SAMEA2635795 qiime_study_2401:Plate16.6 Plate16.6:190 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 190 target_gene 16S rRNA study_center Cornell ERX520618 qiime_experiment_2401:1269672:103 qiime_experiment_2401:1269672:103 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502150 SAMEA2635796 qiime_study_2401:Plate15.50 Plate15.50:103 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 51472 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 103 target_gene 16S rRNA study_center Cornell ERX520245 qiime_experiment_2401:1269835:305 qiime_experiment_2401:1269835:305 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501757 SAMEA2635403 qiime_study_2401:Plate6.23 Plate6.23:305 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87516 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 305 target_gene 16S rRNA study_center Cornell ERX520246 qiime_experiment_2401:1269836:529 qiime_experiment_2401:1269836:529 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501758 SAMEA2635404 qiime_study_2401:Plate9.52 Plate9.52:529 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68451 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 529 target_gene 16S rRNA study_center Cornell ERX520247 qiime_experiment_2401:1269837:293 qiime_experiment_2401:1269837:293 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501759 SAMEA2635405 qiime_study_2401:Plate6.11 Plate6.11:293 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 91431 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 293 target_gene 16S rRNA study_center Cornell ERX520248 qiime_experiment_2401:1269838:89 qiime_experiment_2401:1269838:89 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501760 SAMEA2635406 qiime_study_2401:Plate15.33 Plate15.33:89 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89186 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 89 target_gene 16S rRNA study_center Cornell ERX520249 qiime_experiment_2401:1269839:340 qiime_experiment_2401:1269839:340 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501761 SAMEA2635407 qiime_study_2401:Plate6.60 Plate6.60:340 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 74774 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 340 target_gene 16S rRNA study_center Cornell ERX520250 qiime_experiment_2401:1269840:317 qiime_experiment_2401:1269840:317 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501762 SAMEA2635408 qiime_study_2401:Plate6.36 Plate6.36:317 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78787 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 317 target_gene 16S rRNA study_center Cornell ERX520341 qiime_experiment_2401:1269382:280 qiime_experiment_2401:1269382:280 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501860 SAMEA2635506 qiime_study_2401:Plate5.84 Plate5.84:280 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77880 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 280 target_gene 16S rRNA study_center Cornell ERX520342 qiime_experiment_2401:1269383:75 qiime_experiment_2401:1269383:75 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501861 SAMEA2635507 qiime_study_2401:Plate15.16 Plate15.16:75 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69840 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 75 target_gene 16S rRNA study_center Cornell ERX520343 qiime_experiment_2401:1269384:486 qiime_experiment_2401:1269384:486 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501862 SAMEA2635508 qiime_study_2401:Plate8.51 Plate8.51:486 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71946 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 486 target_gene 16S rRNA study_center Cornell ERX520344 qiime_experiment_2401:1269385:18 qiime_experiment_2401:1269385:18 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501863 SAMEA2635509 qiime_study_2401:Plate14.35 Plate14.35:18 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70371 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 18 target_gene 16S rRNA study_center Cornell ERX520345 qiime_experiment_2401:1269386:92 qiime_experiment_2401:1269386:92 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501864 SAMEA2635510 qiime_study_2401:Plate15.36 Plate15.36:92 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64072 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 92 target_gene 16S rRNA study_center Cornell ERX520346 qiime_experiment_2401:1269387:112 qiime_experiment_2401:1269387:112 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501865 SAMEA2635511 qiime_study_2401:Plate15.6 Plate15.6:112 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80302 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 112 target_gene 16S rRNA study_center Cornell ERX520427 qiime_experiment_2401:1269472:475 qiime_experiment_2401:1269472:475 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501950 SAMEA2635596 qiime_study_2401:Plate8.40 Plate8.40:475 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 121822 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 475 target_gene 16S rRNA study_center Cornell ERX520428 qiime_experiment_2401:1269473:462 qiime_experiment_2401:1269473:462 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501951 SAMEA2635597 qiime_study_2401:Plate8.26 Plate8.26:462 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82100 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 462 target_gene 16S rRNA study_center Cornell ERX520429 qiime_experiment_2401:1269474:226 qiime_experiment_2401:1269474:226 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501952 SAMEA2635598 qiime_study_2401:Plate5.29 Plate5.29:226 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77821 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 226 target_gene 16S rRNA study_center Cornell ERX520430 qiime_experiment_2401:1269475:82 qiime_experiment_2401:1269475:82 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501953 SAMEA2635599 qiime_study_2401:Plate15.23 Plate15.23:82 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63258 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 82 target_gene 16S rRNA study_center Cornell ERX520431 qiime_experiment_2401:1269476:507 qiime_experiment_2401:1269476:507 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501954 SAMEA2635600 qiime_study_2401:Plate9.21 Plate9.21:507 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76403 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 507 target_gene 16S rRNA study_center Cornell ERX520432 qiime_experiment_2401:1269477:517 qiime_experiment_2401:1269477:517 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501955 SAMEA2635601 qiime_study_2401:Plate9.34 Plate9.34:517 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 65700 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 517 target_gene 16S rRNA study_center Cornell ERX520523 qiime_experiment_2401:1269570:537 qiime_experiment_2401:1269570:537 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502048 SAMEA2635694 qiime_study_2401:Plate9.61 Plate9.61:537 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73002 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 537 target_gene 16S rRNA study_center Cornell ERX520524 qiime_experiment_2401:1269571:289 qiime_experiment_2401:1269571:289 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502049 SAMEA2635695 qiime_study_2401:Plate5.93 Plate5.93:289 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80464 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 289 target_gene 16S rRNA study_center Cornell ERX520525 qiime_experiment_2401:1269572:266 qiime_experiment_2401:1269572:266 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502050 SAMEA2635696 qiime_study_2401:Plate5.70 Plate5.70:266 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71949 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 266 target_gene 16S rRNA study_center Cornell ERX520526 qiime_experiment_2401:1269574:431 qiime_experiment_2401:1269574:431 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502052 SAMEA2635698 qiime_study_2401:Plate7.76 Plate7.76:431 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 95138 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 431 target_gene 16S rRNA study_center Cornell ERX520527 qiime_experiment_2401:1269575:434 qiime_experiment_2401:1269575:434 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502053 SAMEA2635699 qiime_study_2401:Plate7.79 Plate7.79:434 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90715 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 434 target_gene 16S rRNA study_center Cornell ERX520528 qiime_experiment_2401:1269576:390 qiime_experiment_2401:1269576:390 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502054 SAMEA2635700 qiime_study_2401:Plate7.27 Plate7.27:390 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78375 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 390 target_gene 16S rRNA study_center Cornell ERX520619 qiime_experiment_2401:1269673:420 qiime_experiment_2401:1269673:420 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502151 SAMEA2635797 qiime_study_2401:Plate7.65 Plate7.65:420 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87490 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 420 target_gene 16S rRNA study_center Cornell ERX520620 qiime_experiment_2401:1269674:285 qiime_experiment_2401:1269674:285 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502152 SAMEA2635798 qiime_study_2401:Plate5.9 Plate5.9:285 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73761 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 285 target_gene 16S rRNA study_center Cornell ERX520621 qiime_experiment_2401:1269675:419 qiime_experiment_2401:1269675:419 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502153 SAMEA2635799 qiime_study_2401:Plate7.64 Plate7.64:419 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 89956 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 419 target_gene 16S rRNA study_center Cornell ERX520622 qiime_experiment_2401:1269676:222 qiime_experiment_2401:1269676:222 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502154 SAMEA2635800 qiime_study_2401:Plate5.24 Plate5.24:222 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71889 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 222 target_gene 16S rRNA study_center Cornell ERX520623 qiime_experiment_2401:1269677:240 qiime_experiment_2401:1269677:240 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502155 SAMEA2635801 qiime_study_2401:Plate5.43 Plate5.43:240 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70810 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 240 target_gene 16S rRNA study_center Cornell ERX520624 qiime_experiment_2401:1269678:264 qiime_experiment_2401:1269678:264 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502156 SAMEA2635802 qiime_study_2401:Plate5.69 Plate5.69:264 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71791 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 264 target_gene 16S rRNA study_center Cornell ERX520251 qiime_experiment_2401:1269841:67 qiime_experiment_2401:1269841:67 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501763 SAMEA2635409 qiime_study_2401:Plate14.96 Plate14.96:67 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 96392 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 67 target_gene 16S rRNA study_center Cornell ERX520252 qiime_experiment_2401:1269842:147 qiime_experiment_2401:1269842:147 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501764 SAMEA2635410 qiime_study_2401:Plate16.16 Plate16.16:147 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 147 target_gene 16S rRNA study_center Cornell ERX520253 qiime_experiment_2401:1269844:488 qiime_experiment_2401:1269844:488 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501766 SAMEA2635412 qiime_study_2401:Plate8.53 Plate8.53:488 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 48567 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 488 target_gene 16S rRNA study_center Cornell ERX520254 qiime_experiment_2401:1269845:338 qiime_experiment_2401:1269845:338 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501767 SAMEA2635413 qiime_study_2401:Plate6.58 Plate6.58:338 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64075 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 338 target_gene 16S rRNA study_center Cornell ERX520255 qiime_experiment_2401:1269846:437 qiime_experiment_2401:1269846:437 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501768 SAMEA2635414 qiime_study_2401:Plate7.82 Plate7.82:437 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80655 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 437 target_gene 16S rRNA study_center Cornell ERX520256 qiime_experiment_2401:1269847:45 qiime_experiment_2401:1269847:45 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501769 SAMEA2635415 qiime_study_2401:Plate14.68 Plate14.68:45 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77890 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 45 target_gene 16S rRNA study_center Cornell ERX520347 qiime_experiment_2401:1269388:166 qiime_experiment_2401:1269388:166 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501866 SAMEA2635512 qiime_study_2401:Plate16.36 Plate16.36:166 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 248945 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 166 target_gene 16S rRNA study_center Cornell ERX520348 qiime_experiment_2401:1269389:66 qiime_experiment_2401:1269389:66 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501867 SAMEA2635513 qiime_study_2401:Plate14.95 Plate14.95:66 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60003 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 66 target_gene 16S rRNA study_center Cornell ERX520349 qiime_experiment_2401:1269390:379 qiime_experiment_2401:1269390:379 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501868 SAMEA2635514 qiime_study_2401:Plate7.14 Plate7.14:379 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 160097 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 379 target_gene 16S rRNA study_center Cornell ERX520350 qiime_experiment_2401:1269391:116 qiime_experiment_2401:1269391:116 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501869 SAMEA2635515 qiime_study_2401:Plate15.63 Plate15.63:116 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58660 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 116 target_gene 16S rRNA study_center Cornell ERX520351 qiime_experiment_2401:1269392:263 qiime_experiment_2401:1269392:263 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501870 SAMEA2635516 qiime_study_2401:Plate5.68 Plate5.68:263 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70171 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 263 target_gene 16S rRNA study_center Cornell ERX520352 qiime_experiment_2401:1269393:491 qiime_experiment_2401:1269393:491 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501871 SAMEA2635517 qiime_study_2401:Plate8.6 Plate8.6:491 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58301 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 491 target_gene 16S rRNA study_center Cornell ERX520433 qiime_experiment_2401:1269478:534 qiime_experiment_2401:1269478:534 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501956 SAMEA2635602 qiime_study_2401:Plate9.59 Plate9.59:534 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75544 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 534 target_gene 16S rRNA study_center Cornell ERX520434 qiime_experiment_2401:1269479:59 qiime_experiment_2401:1269479:59 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501957 SAMEA2635603 qiime_study_2401:Plate14.85 Plate14.85:59 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70056 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 59 target_gene 16S rRNA study_center Cornell ERX520435 qiime_experiment_2401:1269480:441 qiime_experiment_2401:1269480:441 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501958 SAMEA2635604 qiime_study_2401:Plate7.87 Plate7.87:441 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 103304 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 441 target_gene 16S rRNA study_center Cornell ERX520436 qiime_experiment_2401:1269481:9 qiime_experiment_2401:1269481:9 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501959 SAMEA2635605 qiime_study_2401:Plate14.21 Plate14.21:9 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 64931 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 9 target_gene 16S rRNA study_center Cornell ERX520437 qiime_experiment_2401:1269482:314 qiime_experiment_2401:1269482:314 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501960 SAMEA2635606 qiime_study_2401:Plate6.32 Plate6.32:314 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67074 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 314 target_gene 16S rRNA study_center Cornell ERX520438 qiime_experiment_2401:1269483:464 qiime_experiment_2401:1269483:464 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501961 SAMEA2635607 qiime_study_2401:Plate8.28 Plate8.28:464 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 59059 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 464 target_gene 16S rRNA study_center Cornell ERX520529 qiime_experiment_2401:1269577:202 qiime_experiment_2401:1269577:202 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502055 SAMEA2635701 qiime_study_2401:Plate16.70 Plate16.70:202 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 202 target_gene 16S rRNA study_center Cornell ERX520530 qiime_experiment_2401:1269578:275 qiime_experiment_2401:1269578:275 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502056 SAMEA2635702 qiime_study_2401:Plate5.8 Plate5.8:275 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 75852 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 275 target_gene 16S rRNA study_center Cornell ERX520531 qiime_experiment_2401:1269579:329 qiime_experiment_2401:1269579:329 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502057 SAMEA2635703 qiime_study_2401:Plate6.5 Plate6.5:329 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 93727 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 329 target_gene 16S rRNA study_center Cornell ERX520532 qiime_experiment_2401:1269580:368 qiime_experiment_2401:1269580:368 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502058 SAMEA2635704 qiime_study_2401:Plate6.88 Plate6.88:368 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85969 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 368 target_gene 16S rRNA study_center Cornell ERX520533 qiime_experiment_2401:1269581:126 qiime_experiment_2401:1269581:126 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502059 SAMEA2635705 qiime_study_2401:Plate15.8 Plate15.8:126 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 78452 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 126 target_gene 16S rRNA study_center Cornell ERX520534 qiime_experiment_2401:1269582:88 qiime_experiment_2401:1269582:88 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502060 SAMEA2635706 qiime_study_2401:Plate15.32 Plate15.32:88 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 81151 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 88 target_gene 16S rRNA study_center Cornell ERX520625 qiime_experiment_2401:1269679:291 qiime_experiment_2401:1269679:291 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502157 SAMEA2635803 qiime_study_2401:Plate6.1 Plate6.1:291 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87515 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 291 target_gene 16S rRNA study_center Cornell ERX520626 qiime_experiment_2401:1269680:471 qiime_experiment_2401:1269680:471 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502158 SAMEA2635804 qiime_study_2401:Plate8.35 Plate8.35:471 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82847 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 471 target_gene 16S rRNA study_center Cornell ERX520627 qiime_experiment_2401:1269681:397 qiime_experiment_2401:1269681:397 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502159 SAMEA2635805 qiime_study_2401:Plate7.34 Plate7.34:397 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86453 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 397 target_gene 16S rRNA study_center Cornell ERX520628 qiime_experiment_2401:1269683:548 qiime_experiment_2401:1269683:548 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502161 SAMEA2635807 qiime_study_2401:Plate9.78 Plate9.78:548 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 84651 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 548 target_gene 16S rRNA study_center Cornell ERX520629 qiime_experiment_2401:1269684:297 qiime_experiment_2401:1269684:297 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502162 SAMEA2635808 qiime_study_2401:Plate6.16 Plate6.16:297 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 91703 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 297 target_gene 16S rRNA study_center Cornell ERX520630 qiime_experiment_2401:1269685:401 qiime_experiment_2401:1269685:401 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502163 SAMEA2635809 qiime_study_2401:Plate7.39 Plate7.39:401 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86143 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 401 target_gene 16S rRNA study_center Cornell ERX520257 qiime_experiment_2401:1269848:551 qiime_experiment_2401:1269848:551 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501770 SAMEA2635416 qiime_study_2401:Plate9.84 Plate9.84:551 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 41723 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 551 target_gene 16S rRNA study_center Cornell ERX520258 qiime_experiment_2401:1269849:95 qiime_experiment_2401:1269849:95 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501771 SAMEA2635417 qiime_study_2401:Plate15.4 Plate15.4:95 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73538 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 95 target_gene 16S rRNA study_center Cornell ERX520259 qiime_experiment_2401:1269851:306 qiime_experiment_2401:1269851:306 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501773 SAMEA2635419 qiime_study_2401:Plate6.24 Plate6.24:306 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85203 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 306 target_gene 16S rRNA study_center Cornell ERX520260 qiime_experiment_2401:1269852:177 qiime_experiment_2401:1269852:177 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501774 SAMEA2635420 qiime_study_2401:Plate16.47 Plate16.47:177 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 177 target_gene 16S rRNA study_center Cornell ERX520261 qiime_experiment_2401:1269853:530 qiime_experiment_2401:1269853:530 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501775 SAMEA2635421 qiime_study_2401:Plate9.53 Plate9.53:530 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 91984 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 530 target_gene 16S rRNA study_center Cornell ERX520262 qiime_experiment_2401:1269854:414 qiime_experiment_2401:1269854:414 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501776 SAMEA2635422 qiime_study_2401:Plate7.58 Plate7.58:414 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 69797 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 414 target_gene 16S rRNA study_center Cornell ERX520353 qiime_experiment_2401:1269394:100 qiime_experiment_2401:1269394:100 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501872 SAMEA2635518 qiime_study_2401:Plate15.47 Plate15.47:100 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70323 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 100 target_gene 16S rRNA study_center Cornell ERX520354 qiime_experiment_2401:1269395:119 qiime_experiment_2401:1269395:119 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501873 SAMEA2635519 qiime_study_2401:Plate15.7 Plate15.7:119 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62029 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 119 target_gene 16S rRNA study_center Cornell ERX520355 qiime_experiment_2401:1269396:259 qiime_experiment_2401:1269396:259 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501874 SAMEA2635520 qiime_study_2401:Plate5.64 Plate5.64:259 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66902 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 259 target_gene 16S rRNA study_center Cornell ERX520356 qiime_experiment_2401:1269397:181 qiime_experiment_2401:1269397:181 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501875 SAMEA2635521 qiime_study_2401:Plate16.51 Plate16.51:181 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 5 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 181 target_gene 16S rRNA study_center Cornell ERX520357 qiime_experiment_2401:1269398:367 qiime_experiment_2401:1269398:367 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501876 SAMEA2635522 qiime_study_2401:Plate6.87 Plate6.87:367 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83310 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 367 target_gene 16S rRNA study_center Cornell ERX520358 qiime_experiment_2401:1269399:120 qiime_experiment_2401:1269399:120 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501877 SAMEA2635523 qiime_study_2401:Plate15.72 Plate15.72:120 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 67183 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 120 target_gene 16S rRNA study_center Cornell ERX520439 qiime_experiment_2401:1269484:47 qiime_experiment_2401:1269484:47 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501962 SAMEA2635608 qiime_study_2401:Plate14.71 Plate14.71:47 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94096 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 47 target_gene 16S rRNA study_center Cornell ERX520440 qiime_experiment_2401:1269485:384 qiime_experiment_2401:1269485:384 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501963 SAMEA2635609 qiime_study_2401:Plate7.2 Plate7.2:384 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90343 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 384 target_gene 16S rRNA study_center Cornell ERX520441 qiime_experiment_2401:1269486:349 qiime_experiment_2401:1269486:349 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501964 SAMEA2635610 qiime_study_2401:Plate6.7 Plate6.7:349 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82445 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 349 target_gene 16S rRNA study_center Cornell ERX520442 qiime_experiment_2401:1269487:538 qiime_experiment_2401:1269487:538 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501965 SAMEA2635611 qiime_study_2401:Plate9.62 Plate9.62:538 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 41561 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 538 target_gene 16S rRNA study_center Cornell ERX520443 qiime_experiment_2401:1269488:286 qiime_experiment_2401:1269488:286 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501966 SAMEA2635612 qiime_study_2401:Plate5.90 Plate5.90:286 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83082 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 286 target_gene 16S rRNA study_center Cornell ERX520444 qiime_experiment_2401:1269489:543 qiime_experiment_2401:1269489:543 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501967 SAMEA2635613 qiime_study_2401:Plate9.70 Plate9.70:543 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66428 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 543 target_gene 16S rRNA study_center Cornell ERX520535 qiime_experiment_2401:1269583:487 qiime_experiment_2401:1269583:487 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502061 SAMEA2635707 qiime_study_2401:Plate8.52 Plate8.52:487 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 94972 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 487 target_gene 16S rRNA study_center Cornell ERX520536 qiime_experiment_2401:1269584:399 qiime_experiment_2401:1269584:399 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502062 SAMEA2635708 qiime_study_2401:Plate7.36 Plate7.36:399 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85443 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 399 target_gene 16S rRNA study_center Cornell ERX520537 qiime_experiment_2401:1269585:32 qiime_experiment_2401:1269585:32 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502063 SAMEA2635709 qiime_study_2401:Plate14.54 Plate14.54:32 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 62874 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 32 target_gene 16S rRNA study_center Cornell ERX520538 qiime_experiment_2401:1269586:481 qiime_experiment_2401:1269586:481 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502064 SAMEA2635710 qiime_study_2401:Plate8.47 Plate8.47:481 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 83889 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 481 target_gene 16S rRNA study_center Cornell ERX520539 qiime_experiment_2401:1269587:145 qiime_experiment_2401:1269587:145 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502065 SAMEA2635711 qiime_study_2401:Plate16.14 Plate16.14:145 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 2 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 145 target_gene 16S rRNA study_center Cornell ERX520540 qiime_experiment_2401:1269588:502 qiime_experiment_2401:1269588:502 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502066 SAMEA2635712 qiime_study_2401:Plate8.9 Plate8.9:502 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 57667 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 502 target_gene 16S rRNA study_center Cornell ERX520631 qiime_experiment_2401:1269688:118 qiime_experiment_2401:1269688:118 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502166 SAMEA2635812 qiime_study_2401:Plate15.68 Plate15.68:118 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 48276 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 118 target_gene 16S rRNA study_center Cornell ERX520632 qiime_experiment_2401:1269689:301 qiime_experiment_2401:1269689:301 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502167 SAMEA2635813 qiime_study_2401:Plate6.2 Plate6.2:301 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 86659 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 301 target_gene 16S rRNA study_center Cornell ERX520633 qiime_experiment_2401:1269690:6 qiime_experiment_2401:1269690:6 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502168 SAMEA2635814 qiime_study_2401:Plate14.19 Plate14.19:6 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 70848 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 6 target_gene 16S rRNA study_center Cornell ERX520634 qiime_experiment_2401:1269691:479 qiime_experiment_2401:1269691:479 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502169 SAMEA2635815 qiime_study_2401:Plate8.44 Plate8.44:479 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 210994 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 479 target_gene 16S rRNA study_center Cornell ERX520635 qiime_experiment_2401:1269692:29 qiime_experiment_2401:1269692:29 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502170 SAMEA2635816 qiime_study_2401:Plate14.5 Plate14.5:29 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 95573 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 29 target_gene 16S rRNA study_center Cornell ERX520636 qiime_experiment_2401:1269693:443 qiime_experiment_2401:1269693:443 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502171 SAMEA2635817 qiime_study_2401:Plate7.89 Plate7.89:443 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 71239 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 443 target_gene 16S rRNA study_center Cornell ERX520263 qiime_experiment_2401:1269855:290 qiime_experiment_2401:1269855:290 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501777 SAMEA2635423 qiime_study_2401:Plate5.94 Plate5.94:290 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 96262 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 290 target_gene 16S rRNA study_center Cornell ERX520264 qiime_experiment_2401:1269856:115 qiime_experiment_2401:1269856:115 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501778 SAMEA2635424 qiime_study_2401:Plate15.62 Plate15.62:115 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 56833 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 115 target_gene 16S rRNA study_center Cornell ERX520265 qiime_experiment_2401:1269857:23 qiime_experiment_2401:1269857:23 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501779 SAMEA2635425 qiime_study_2401:Plate14.42 Plate14.42:23 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63927 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 23 target_gene 16S rRNA study_center Cornell ERX520266 qiime_experiment_2401:1269858:457 qiime_experiment_2401:1269858:457 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501780 SAMEA2635426 qiime_study_2401:Plate8.2 Plate8.2:457 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 47451 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 457 target_gene 16S rRNA study_center Cornell ERX520267 qiime_experiment_2401:1269859:2 qiime_experiment_2401:1269859:2 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501781 SAMEA2635427 qiime_study_2401:Plate14.15 Plate14.15:2 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 44253 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 2 target_gene 16S rRNA study_center Cornell ERX520268 qiime_experiment_2401:1269860:79 qiime_experiment_2401:1269860:79 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501782 SAMEA2635428 qiime_study_2401:Plate15.2 Plate15.2:79 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66861 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 79 target_gene 16S rRNA study_center Cornell ERX520359 qiime_experiment_2401:1269400:426 qiime_experiment_2401:1269400:426 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501878 SAMEA2635524 qiime_study_2401:Plate7.71 Plate7.71:426 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 99090 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 426 target_gene 16S rRNA study_center Cornell ERX520360 qiime_experiment_2401:1269401:512 qiime_experiment_2401:1269401:512 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501879 SAMEA2635525 qiime_study_2401:Plate9.28 Plate9.28:512 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 95420 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 512 target_gene 16S rRNA study_center Cornell ERX520361 qiime_experiment_2401:1269402:210 qiime_experiment_2401:1269402:210 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501880 SAMEA2635526 qiime_study_2401:Plate5.10 Plate5.10:210 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90595 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 210 target_gene 16S rRNA study_center Cornell ERX520362 qiime_experiment_2401:1269404:531 qiime_experiment_2401:1269404:531 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501882 SAMEA2635528 qiime_study_2401:Plate9.54 Plate9.54:531 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68601 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 531 target_gene 16S rRNA study_center Cornell ERX520363 qiime_experiment_2401:1269405:492 qiime_experiment_2401:1269405:492 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501883 SAMEA2635529 qiime_study_2401:Plate8.60 Plate8.60:492 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/4/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 40251 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 492 target_gene 16S rRNA study_center Cornell ERX520364 qiime_experiment_2401:1269406:318 qiime_experiment_2401:1269406:318 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501884 SAMEA2635530 qiime_study_2401:Plate6.38 Plate6.38:318 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 68190 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 318 target_gene 16S rRNA study_center Cornell ERX520445 qiime_experiment_2401:1269490:522 qiime_experiment_2401:1269490:522 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501968 SAMEA2635614 qiime_study_2401:Plate9.42 Plate9.42:522 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/19/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 76026 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 522 target_gene 16S rRNA study_center Cornell ERX520446 qiime_experiment_2401:1269491:298 qiime_experiment_2401:1269491:298 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501969 SAMEA2635615 qiime_study_2401:Plate6.17 Plate6.17:298 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 77975 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 298 target_gene 16S rRNA study_center Cornell ERX520447 qiime_experiment_2401:1269492:287 qiime_experiment_2401:1269492:287 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501970 SAMEA2635616 qiime_study_2401:Plate5.91 Plate5.91:287 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 73717 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 287 target_gene 16S rRNA study_center Cornell ERX520448 qiime_experiment_2401:1269493:212 qiime_experiment_2401:1269493:212 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501971 SAMEA2635617 qiime_study_2401:Plate5.13 Plate5.13:212 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 85330 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 212 target_gene 16S rRNA study_center Cornell ERX520449 qiime_experiment_2401:1269494:150 qiime_experiment_2401:1269494:150 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501972 SAMEA2635618 qiime_study_2401:Plate16.20 Plate16.20:150 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 5 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 150 target_gene 16S rRNA study_center Cornell ERX520450 qiime_experiment_2401:1269495:310 qiime_experiment_2401:1269495:310 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS501973 SAMEA2635619 qiime_study_2401:Plate6.29 Plate6.29:310 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/31/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 63930 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 310 target_gene 16S rRNA study_center Cornell ERX520541 qiime_experiment_2401:1269589:87 qiime_experiment_2401:1269589:87 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502067 SAMEA2635713 qiime_study_2401:Plate15.30 Plate15.30:87 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 66194 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 87 target_gene 16S rRNA study_center Cornell ERX520542 qiime_experiment_2401:1269590:435 qiime_experiment_2401:1269590:435 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502068 SAMEA2635714 qiime_study_2401:Plate7.8 Plate7.8:435 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 90034 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 435 target_gene 16S rRNA study_center Cornell ERX520543 qiime_experiment_2401:1269591:44 qiime_experiment_2401:1269591:44 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502069 SAMEA2635715 qiime_study_2401:Plate14.67 Plate14.67:44 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 91211 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 44 target_gene 16S rRNA study_center Cornell ERX520544 qiime_experiment_2401:1269592:157 qiime_experiment_2401:1269592:157 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502070 SAMEA2635716 qiime_study_2401:Plate16.27 Plate16.27:157 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 8/14/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 1 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 157 target_gene 16S rRNA study_center Cornell ERX520545 qiime_experiment_2401:1269593:255 qiime_experiment_2401:1269593:255 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502071 SAMEA2635717 qiime_study_2401:Plate5.59 Plate5.59:255 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 1/25/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 82147 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 255 target_gene 16S rRNA study_center Cornell ERX520546 qiime_experiment_2401:1269594:60 qiime_experiment_2401:1269594:60 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502072 SAMEA2635718 qiime_study_2401:Plate14.86 Plate14.86:60 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 58099 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 60 target_gene 16S rRNA study_center Cornell ERX520637 qiime_experiment_2401:1269694:58 qiime_experiment_2401:1269694:58 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502172 SAMEA2635818 qiime_study_2401:Plate14.83 Plate14.83:58 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 60892 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 58 target_gene 16S rRNA study_center Cornell ERX520638 qiime_experiment_2401:1269695:418 qiime_experiment_2401:1269695:418 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502173 SAMEA2635819 qiime_study_2401:Plate7.63 Plate7.63:418 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 92444 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 418 target_gene 16S rRNA study_center Cornell ERX520639 qiime_experiment_2401:1269696:70 qiime_experiment_2401:1269696:70 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502174 SAMEA2635820 qiime_study_2401:Plate15.11 Plate15.11:70 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 56567 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 70 target_gene 16S rRNA study_center Cornell ERX520640 qiime_experiment_2401:1269697:14 qiime_experiment_2401:1269697:14 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502175 SAMEA2635821 qiime_study_2401:Plate14.30 Plate14.30:14 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/1/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 80059 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 14 target_gene 16S rRNA study_center Cornell ERX520641 qiime_experiment_2401:1269699:377 qiime_experiment_2401:1269699:377 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502177 SAMEA2635823 qiime_study_2401:Plate7.12 Plate7.12:377 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 2/2/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 87119 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 377 target_gene 16S rRNA study_center Cornell ERX520642 qiime_experiment_2401:1269700:127 qiime_experiment_2401:1269700:127 ERP006342 qiime_study_2401 fecal samples from TwinsUK MiSeq runs ERS502178 SAMEA2635824 qiime_study_2401:Plate15.80 Plate15.80:127 AMPLICON METAGENOMIC PCR This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. unspecified experiment_title TwinsUK Miseq target_subfragment V4 sample_center Kings College London sequencing_meth sequencing by synthesis run_date 4/3/13 experiment_center Cornell pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT region V4 run_center Cornell num_sequences 105162 platform Illumina library_construction_protocol This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions. experiment_design_description fecal samples from TwinsUK MiSeq runs row_number 127 target_gene 16S rRNA study_center Cornell