ERX528573 E-MTAB-2787:Control_B_ACTTGA_L001_R1_001 E-MTAB-2787:Control_B_ACTTGA_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513151 SAMEA2665140 E-MTAB-2787:Control_B_ACTTGA_L001_R1_001 Control_B_ACTTGA_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 0 hour Experimental Factor: phenotype no overexpression of MYCN ERX528574 E-MTAB-2787:24hr_Dox_A_ATCACG_L001_R1_001 E-MTAB-2787:24hr_Dox_A_ATCACG_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513152 SAMEA2665141 E-MTAB-2787:24hr_Dox_A_ATCACG_L001_R1_001 24hr_Dox_A_ATCACG_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 24 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN ERX528575 E-MTAB-2787:1hr_Dox_A_TTAGGC_L001_R1_001 E-MTAB-2787:1hr_Dox_A_TTAGGC_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513153 SAMEA2665142 E-MTAB-2787:1hr_Dox_A_TTAGGC_L001_R1_001 1hr_Dox_A_TTAGGC_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 1 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN ERX528576 E-MTAB-2787:1hr_Dox_B_CAGATC_L001_R1_001 E-MTAB-2787:1hr_Dox_B_CAGATC_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513154 SAMEA2665143 E-MTAB-2787:1hr_Dox_B_CAGATC_L001_R1_001 1hr_Dox_B_CAGATC_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 1 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN ERX528577 E-MTAB-2787:4hr_Dox_B_GCCAAT_L001_R1_001 E-MTAB-2787:4hr_Dox_B_GCCAAT_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513155 SAMEA2665144 E-MTAB-2787:4hr_Dox_B_GCCAAT_L001_R1_001 4hr_Dox_B_GCCAAT_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 4 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN ERX528578 E-MTAB-2787:24hr_Dox_B_ACAGTG_L001_R1_001 E-MTAB-2787:24hr_Dox_B_ACAGTG_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513156 SAMEA2665145 E-MTAB-2787:24hr_Dox_B_ACAGTG_L001_R1_001 24hr_Dox_B_ACAGTG_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 24 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN ERX528579 E-MTAB-2787:Control_A_TGACCA_L001_R1_001 E-MTAB-2787:Control_A_TGACCA_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513157 SAMEA2665146 E-MTAB-2787:Control_A_TGACCA_L001_R1_001 Control_A_TGACCA_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 0 hour Experimental Factor: phenotype no overexpression of MYCN ERX528580 E-MTAB-2787:4hr_Dox_A_CGATGT_L001_R1_001 E-MTAB-2787:4hr_Dox_A_CGATGT_L001_R1_001 ERP006427 E-MTAB-2787 MYCN regulated miRNAs ERS513158 SAMEA2665147 E-MTAB-2787:4hr_Dox_A_CGATGT_L001_R1_001 4hr_Dox_A_CGATGT_L001_R1_001 miRNA-Seq TRANSCRIPTOMIC size fractionation Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina). Illumina Genome Analyzer IIx Experimental Factor: time 4 hour Experimental Factor: phenotype doxycycline-mediated overexpression of MYCN