ERX5124494 E-MTAB-10131:Sample 1_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806302 SAMEA8119322 Sample 1_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Patras ERX5124495 E-MTAB-10131:Sample 10_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806303 SAMEA8119323 Sample 10_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Primepi ERX5124496 E-MTAB-10131:Sample 11_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806304 SAMEA8119324 Sample 11_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Primepi ERX5124497 E-MTAB-10131:Sample 12_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806305 SAMEA8119325 Sample 12_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Primepi ERX5124498 E-MTAB-10131:Sample 13_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806306 SAMEA8119326 Sample 13_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Astoria ERX5124499 E-MTAB-10131:Sample 14_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806307 SAMEA8119327 Sample 14_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Astoria ERX5124500 E-MTAB-10131:Sample 15_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806308 SAMEA8119328 Sample 15_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Astoria ERX5124501 E-MTAB-10131:Sample 16_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806309 SAMEA8119329 Sample 16_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Astoria ERX5124502 E-MTAB-10131:Sample 17_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806310 SAMEA8119330 Sample 17_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Astoria ERX5124503 E-MTAB-10131:Sample 18_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806311 SAMEA8119331 Sample 18_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Astoria ERX5124504 E-MTAB-10131:Sample 19_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806312 SAMEA8119332 Sample 19_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Grana ERX5124505 E-MTAB-10131:Sample 2_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806313 SAMEA8119333 Sample 2_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Patras ERX5124506 E-MTAB-10131:Sample 20_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806314 SAMEA8119334 Sample 20_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Grana ERX5124507 E-MTAB-10131:Sample 21_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806315 SAMEA8119335 Sample 21_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Grana ERX5124508 E-MTAB-10131:Sample 22_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806316 SAMEA8119336 Sample 22_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Grana ERX5124509 E-MTAB-10131:Sample 23_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806317 SAMEA8119337 Sample 23_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Grana ERX5124510 E-MTAB-10131:Sample 24_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806318 SAMEA8119338 Sample 24_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Grana ERX5124511 E-MTAB-10131:Sample 3_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806319 SAMEA8119339 Sample 3_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Patras ERX5124512 E-MTAB-10131:Sample 4_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806320 SAMEA8119340 Sample 4_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Patras ERX5124513 E-MTAB-10131:Sample 5_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806321 SAMEA8119341 Sample 5_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Patras ERX5124514 E-MTAB-10131:Sample 6_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806322 SAMEA8119342 Sample 6_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage tetrad stage Experimental Factor: cultivar Patras ERX5124515 E-MTAB-10131:Sample 7_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806323 SAMEA8119343 Sample 7_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Primepi ERX5124516 E-MTAB-10131:Sample 8_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806324 SAMEA8119344 Sample 8_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Primepi ERX5124517 E-MTAB-10131:Sample 9_p ERP127176 PRJEB43230 Identification of Rf genes in hexaploid wheat (Triticum aestivum L.) by RNA-seq. ERS5806325 SAMEA8119345 Sample 9_p RNA-Seq TRANSCRIPTOMIC RANDOM Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Illumina HiSeq 4000 Experimental Factor: developmental stage late uninucleate microspore stage Experimental Factor: cultivar Primepi