ERS5806302 SAMEA8119322 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119322 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 1 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 1 ERS5806303 SAMEA8119323 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119323 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 10 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 10 ERS5806304 SAMEA8119324 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119324 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 11 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 11 ERS5806305 SAMEA8119325 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119325 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 12 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 12 ERS5806306 SAMEA8119326 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119326 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 13 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 13 ERS5806307 SAMEA8119327 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119327 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 14 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 14 ERS5806308 SAMEA8119328 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119328 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 15 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 15 ERS5806309 SAMEA8119329 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119329 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 16 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 16 ERS5806310 SAMEA8119330 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119330 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 17 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 17 ERS5806311 SAMEA8119331 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119331 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 18 age 3 broker name ArrayExpress common name bread wheat cultivar Astoria developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 18 ERS5806312 SAMEA8119332 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119332 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 19 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 19 ERS5806313 SAMEA8119333 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119333 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 2 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 2 ERS5806314 SAMEA8119334 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119334 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 20 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 20 ERS5806315 SAMEA8119335 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119335 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 21 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 21 ERS5806316 SAMEA8119336 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119336 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 22 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 22 ERS5806317 SAMEA8119337 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119337 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 23 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 23 ERS5806318 SAMEA8119338 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119338 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 24 age 3 broker name ArrayExpress common name bread wheat cultivar Grana developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 24 ERS5806319 SAMEA8119339 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119339 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 3 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 3 ERS5806320 SAMEA8119340 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119340 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 4 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 4 ERS5806321 SAMEA8119341 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119341 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 5 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 5 ERS5806322 SAMEA8119342 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119342 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 6 age 3 broker name ArrayExpress common name bread wheat cultivar Patras developmental stage tetrad stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 6 ERS5806323 SAMEA8119343 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119343 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 7 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 7 ERS5806324 SAMEA8119344 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119344 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 8 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 8 ERS5806325 SAMEA8119345 4565 Triticum aestivum Protocols: Anthers were harvested from spikes at tetrad and late uninucleate microspore stages after completing of ear emergence (59 Zadox growth scale). Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana ) and two restorers (Patres and Primépi ), were grown in growth chamber (ForClean, ZalMed Sp. z.o.o) under the following conditions: temperature - 18˚C/15˚C day/night, 14/10 h photoperiod and constant relative humidity of 50%. Plants were cultivated in pots filled with a mixture of peat and perlite (1:1, v/v), three plants per single pot (19 cm diameter). The plants were hand irrigated daily to maintain soil water content close to field capacity. GeneMATRIX Universal RNA Purification kit (EURx, Gdańsk, Poland) was used for total RNA isolation from the anthers of two fertility restoring and non-restoring wheat cultivars according to the manufacturer's protocol. The quantity and purity of each RNA sample was determined by the absorbance (Abs) at 260 and 280 nm by Nanodrop 2000 spectrophotometer and was measured using a Qubit® 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). RNA Integrity Number (RIN) of the total RNA was assessed using the BioAnalyzer 2100, Plant RNA Pico or RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA from harvested anthers was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Twenty four strand-specific cDNA libraries for four cultivars, two anthers' developmental stages, and three biological replications were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). ENA first public 2021-06-30 ENA last update 2021-06-30 External Id SAMEA8119345 INSDC center alias Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC center name Department of Biotechnology and Bioinformatics The Faculty of Chemistry Rzeszow University of Technology INSDC first public 2021-06-30T00:26:50Z INSDC last update 2021-06-30T00:26:50Z INSDC status public Submitter Id E-MTAB-10131:Sample 9 age 3 broker name ArrayExpress common name bread wheat cultivar Primepi developmental stage late uninucleate microspore stage genotype wild type genotype organism part anther sample name E-MTAB-10131:Sample 9